Protein Alignment Sema2b and sema4ba

DIOPT Version :10

• Sequence 1: NP_001261024.1
• Gene: Sema2b / 246538
• FlyBaseID: FBgn0264273
• Length: 824
• Species: Drosophila melanogaster
• Sequence 2: NP_001121824.1
• Gene: sema4ba / 572261
• ZFINID: ZDB-GENE-070705-31
• Length: 830
• Species: Danio rerio
• Alignment Length: 769
• Identity: 222/769 - (28%)
• Similarity: 353/769 - (45%)
• Gaps: 161/769 - (20%)

  Fly   118 GPLHYKTFYMDERNNALYVGAMDRIFRLNLRNISQSICERDVLILEPTGS-DILNCVSKGKREKV 181
            |..:|.:..:.:.:|.|||||.:.:|.||:.:||::..:|::....|... |  .|..|||..|.
Zfish    52 GVFNYTSLLLSKEDNMLYVGARETLFALNISDISRTQMQRNLTWSTPQRKRD--ECSFKGKDLKT 114

  Fly   182 ECRNHIRVIQPMNFNGQKLYVCGTNAHNP-KDYVINANLTH--LPRSQYVPGIGL-----GIGKC 238
            :|.|:|:::  :..|...||||||.|.:| ..|:::.|.::  |.|::    :|.     |..:|
Zfish   115 DCFNYIKIL--LRVNSTHLYVCGTYAFSPICTYIVSTNTSNFSLVRNE----VGEIMTEDGRSRC 173

  Fly   239 PYDPADNSTAVYVENGNPFGLPALYAGTNAEFTKADSVIFRSDLYNLTNGRKEANFKRTVKYDSK 303
            ||:|...|||:..:.       .|||||.:.|...|.:|::|    |..|       ..:|.::.
Zfish   174 PYNPEYKSTAIMADG-------ELYAGTVSNFQGNDPIIYKS----LGQG-------TALKTENS 220

  Fly   304 L--LDKPNFVGSFEIGEF------------VYFFFREHAVEYINCGKAVYSRVARVCKNDRGGKY 354
            |  |..|.||||..|.|.            :||||.|...|:......:.||:|||||.|:||:.
Zfish   221 LNWLQDPAFVGSAYIQESLPKGNAVGDDDKIYFFFSEAGKEFDFFDNTIVSRIARVCKGDKGGER 285

  Fly   355 MISQNWATYLKARMNCSISSE-FPFYFNEIQSVYKM-PTDD----TKFYATFTTN-TNGLIG-SA 411
            ::.:.|.|:|||::.||:..: ||  ||.||.::.: |:.:    |.||..||:. ..|..| ||
Zfish   286 VLQKKWTTFLKAQLLCSLPDDGFP--FNIIQDMFVLTPSKEDWKKTMFYGVFTSQWYKGASGSSA 348

  Fly   412 VCSYDIRDINAAFDGKFKEQATSNSAWLPVLNSKVPEPRPGTCHNDTAT---------LPDSVLN 467
            ||::.:..:..||:|:::|.......|. ..|..|||||||.|..:||.         :||.|||
Zfish   349 VCAFTMDQVEEAFNGRYREVNRETQQWY-TYNHPVPEPRPGACITNTARAQGISSSLHMPDRVLN 412

  Fly   468 FIRKHPLMDKAVDHEFGNPVFFKRDVILTKLVVDKIRIDKLNQEFLVYFVATTSGHIYKIVQFMH 532
            |::.|.|||..:..:   |:..||:|..|:|.|.  |:..|::.:.|.|:.|..|.|:|.:...:
Zfish   413 FVKDHFLMDNVIRSQ---PLLLKRNVRYTQLAVH--RVQALHKTYDVLFIGTDDGRIHKAINVKN 472

  Fly   533 YGQRHSNLVDIFEASPHSEPIREMTLSHKTGSLYVATDHQVKQIDIAMCARRYDSCFRCV--SDP 595
            .......|| ||   |..:.::.:.|..:.|.|:|::...:.:|.:|.|: .|.||..||  .||
Zfish   473 KMYIIEELV-IF---PEPQSVQHIELDDEKGLLFVSSYSGLVEIPLANCS-NYQSCGECVLSRDP 532

  Fly   596 YCGWD-------KDVNACRPYQLGLLQDVANETSGICDTSVLRK---KVTSSYGQTLHLSCFVKM 650
            ||.|.       |..::...:|    |||.:..:.||.....|.   :|.:.:...:..:.|..:
Zfish   533 YCAWTGKQCSNVKHASSKSQWQ----QDVEDANTAICKMPSPRSGRPRVPACHVVEIPANTFTVL 593

  Fly   651 PEVLRKK--QTRW-YHHSTEKGRYEVRYTPTKYIDTNEGGLVLLAVNEGDGGRYDSYL----DGT 708
            |..||..  ..|| |..:.|:..|          .:.:||||::|..:    |.::|.    :|.
Zfish   594 PCKLRSNLAARRWEYSDTAERFVY----------PSPDGGLVVVAQVD----RQENYACWSEEGN 644

  Fly   709 ---LLCSYGVTVDAHRCSP-----------------PSQKQDYQKIYSHWCNE------------ 741
               ||.||.|..: |..:.                 |.:....||..::| ||
Zfish   645 YRQLLASYCVKAE-HESTTLTGRSRTQQVTQGETILPGETWSPQKTKTYW-NELIVVCALLVFSL 707

  Fly   742 -----FEKYKS--AMKQWQAKQEQC-GLKDKTGPISSNGKHVNDVFSNDALVXP 787
                 |..|::  .||. ..|:.:| .::.|...|.  |||...:..|...: |
Zfish   708 VVLSLFVAYRNRDRMKS-MLKEGECPNMQQKKPRIP--GKHTESLPLNGNNIPP 758

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Sema2b	NP_001261024.1	Sema_2A	120..579	CDD:200499	158/498 (32%)
		PSI	583..>606	CDD:396154	11/31 (35%)
		Ig	632..724	CDD:472250	24/104 (23%)
		Ig strand B	642..646	CDD:409353	0/3 (0%)
		Ig strand C	658..662	CDD:409353	2/4 (50%)
		Ig strand E	686..690	CDD:409353	3/3 (100%)
		Ig strand F	700..705	CDD:409353	1/4 (25%)
		Ig strand G	717..720	CDD:409353	0/2 (0%)
sema4ba	NP_001121824.1	Sema_4B	47..515	CDD:200518	159/500 (32%)
		PSI	516..565	CDD:214655	16/53 (30%)
		Ig	578..654	CDD:472250	21/89 (24%)
		Ig strand B	591..595	CDD:409353	0/3 (0%)
		Ig strand C	603..607	CDD:409353	1/3 (33%)
		Ig strand E	621..625	CDD:409353	2/3 (67%)
		Ig strand F	635..640	CDD:409353	1/4 (25%)