Protein Alignment Sema2b and Sema5b

DIOPT Version :10

• Sequence 1: NP_001261024.1
• Gene: Sema2b / 246538
• FlyBaseID: FBgn0264273
• Length: 824
• Species: Drosophila melanogaster
• Sequence 2: NP_001402064.1
• Gene: Sema5b / 303901
• RGDID: 1308914
• Length: 1093
• Species: Rattus norvegicus
• Alignment Length: 762
• Identity: 230/762 - (30%)
• Similarity: 347/762 - (45%)
• Gaps: 153/762 - (20%)

  Fly    64 ALLLVLVAQTVHVEYARADYENTWNLYYEPPCCTGSSAAHHLRHH--------KEHVQDFSC-GP 119
            ||.|:|.:.|:.:.:.....:.|.....|...||       .|.|        |..|.:|:. |.
  Rat     8 ALSLLLPSFTLLLSHLSTSQDITSEPSSEQQLCT-------RREHPIVAFEDLKPWVSNFTYPGV 65

  Fly   120 LHYKTFYMDERNNALYVGAMDRIFRLNLRNISQSICERDVLILEPT----GSDI-LNCVSKGKRE 179
            ..:....:|...|.|.|||.:.:|||:|.|:|         :|:.|    ..|. .:|.||||.|
  Rat    66 RDFSQLALDPSRNQLIVGARNYLFRLSLANVS---------LLQATEWASSEDTRRSCQSKGKTE 121

  Fly   180 KVECRNHIRVIQPMNFNGQKLYVCGTNAHNPKDYVINANLTHLPRSQYVPGIGLGIGKCPYDPAD 244
            : ||:|::||   :..:|:|:::|||||.:|  ...:..:.:|.|:  :..|. |:.:|||||..
  Rat   122 E-ECQNYVRV---LIVSGRKVFMCGTNAFSP--VCSSRQVGNLSRA--IEKIN-GVARCPYDPRH 177

  Fly   245 NSTAVYVENGNPFGLPALYAGTNAEFTKADSVIFRSDLYNLTNGRKEANFKRTVKYDSKLLDKPN 309
            |||||....|.      |||.|..:|:..|..|:||    |.:|..    .||.:|:||.|::||
  Rat   178 NSTAVISSQGE------LYAATVIDFSGRDPAIYRS----LGSGPP----LRTAQYNSKWLNEPN 228

  Fly   310 FVGSFEIGEFVYFFFREHAVEYINCGKAVYSRVARVCKNDRGGKYMISQNWATYLKARMNCSISS 374
            ||.:|:||.|.|||.||:|||: :||:.||||||||||||.||::::...|.|::|||:|||...
  Rat   229 FVAAFDIGLFAYFFLRENAVEH-DCGRTVYSRVARVCKNDVGGRFLLEDTWTTFMKARLNCSRPG 292

  Fly   375 EFPFYFNEIQSVYKMPTDDTKFYATFTTNTNGLIGSAVCSYDIRDINAAFDGKFKEQATSNSAWL 439
            |.|||:||:||.:.:|..|. .|..||||.|.:..||||::::..|:.||:|.|:.|....:|||
  Rat   293 EVPFYYNELQSAFHLPEQDL-IYGVFTTNVNSIAASAVCAFNLSAISKAFNGPFRYQENPRAAWL 356

  Fly   440 PVLNSKVPEPR------PGTCHNDTAT---LPDSVLNFIRKHPLMDKAVDHEFGNPVFFKRDVIL 495
            |:.|   |.|.      |.|..|:..|   |.|:...|     ||.:||......|...:..|..
  Rat   357 PIAN---PIPNFQCGTLPETGPNENLTERSLQDAQRLF-----LMSEAVQPVTPEPCVTQDSVRF 413

  Fly   496 TKLVVDKIRI-DKLNQEFLVYFVATTSGHIYKIVQFMHYGQRHSNLVDIFEASP-HSEPIREMTL 558
            :.||||.::. |.|   :.|.::.|.||.|.|.:.......|...|.::....| ..||:|.:.:
  Rat   414 SHLVVDLVQAKDTL---YHVLYIGTESGTILKALSTASRSLRGCYLEELHVLPPGRLEPLRSLRI 475

  Fly   559 SHKTGSLYVATDHQVKQIDIAMCARRYDSCFRCVS--DPYCGWDKDVNACRPYQLGLLQDVANET 621
            .|...:|||....:|.::.:..|: .|.|...|:.  |||||||.....|     ..|:|.:|
  Rat   476 LHSARALYVGLSDRVLRVPLERCS-AYLSQGACLGARDPYCGWDGKRQLC-----STLEDSSN-- 532

  Fly   622 SGICDTSVLRKKVTSSYGQTLHLSCFVKMPEVLRKKQTRWYHHSTEKGRYE--VRYTPTKYIDTN 684
                 .|:..:.:|         :|.|:              :.|..|.:.  ..:.|.:::|.:
  Rat   533 -----MSLWIQNIT---------ACPVR--------------NVTRDGGFGPWSPWKPCEHLDGD 569

  Fly   685 EGGLVLLAVNEGDGGR--------------------------YDSYLDGTLLCSYGVTVDAHRCS 723
            ..|..|......|..|                          :.|:...:..|..|..|....||
  Rat   570 NSGSCLCRARSCDSPRPRCGGLECLGPAIHIANCSRNGAWTAWSSWAQCSTSCGIGFQVRQRSCS 634

  Fly   724 PPSQKQD-----YQKIYSHWCNE-----FEKYKSAMKQWQAKQEQCG 760
            .|:.:..     .:.....:|||     ...:.::...|......||
  Rat   635 NPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSSNCG 681

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Sema2b	NP_001261024.1	Sema_2A	120..579	CDD:200499	175/474 (37%)
		PSI	583..>606	CDD:396154	10/24 (42%)
		Ig	632..724	CDD:472250	16/119 (13%)
		Ig strand B	642..646	CDD:409353	0/3 (0%)
		Ig strand C	658..662	CDD:409353	0/3 (0%)
		Ig strand E	686..690	CDD:409353	1/3 (33%)
		Ig strand F	700..705	CDD:409353	2/30 (7%)
		Ig strand G	717..720	CDD:409353	1/2 (50%)
Sema5b	NP_001402064.1	Sema	60..496	CDD:472829	177/480 (37%)
		PSI	497..544	CDD:396154	17/68 (25%)
		TSP1	609..662	CDD:214559	10/52 (19%)
		TSP1	667..713	CDD:214559	3/15 (20%)
		TSP1	798..850	CDD:214559
		TSP1	855..907	CDD:214559
		TSP_1	911..952	CDD:459668