Protein Alignment Sema2b and Sema4b

DIOPT Version :10

• Sequence 1: NP_001261024.1
• Gene: Sema2b / 246538
• FlyBaseID: FBgn0264273
• Length: 824
• Species: Drosophila melanogaster
• Sequence 2: NP_001163933.1
• Gene: Sema4b / 293042
• RGDID: 1307872
• Length: 821
• Species: Rattus norvegicus
• Alignment Length: 899
• Identity: 238/899 - (26%)
• Similarity: 359/899 - (39%)
• Gaps: 221/899 - (24%)

  Fly    53 GKGKHRLCSLSAL-LLVLVAQTVHVEYARADYENTWNLYYEPPCCTGSSAAHHLRHHKEHVQDFS 116
            |:.......|||| ||:|:.:|.   .|||               .|...:..|...:..::.|.
  Rat     2 GRASRSADLLSALQLLLLLLRTT---TARA---------------LGPRISVPLDSEERLIRKFE 48

  Fly   117 CGPL-HYKTFYMDERNNALYVGAMDRIFRLNLRNISQSICERDVLILEPTG--SDIL-------- 170
            ...: :|....:.:....|||||.:.:|.|| .|:|          ..|.|  .::|
  Rat    49 AENISNYTALLLSQDGKTLYVGAREALFALN-SNLS----------FLPGGEYQELLWSADPDRK 102

  Fly   171 -NCVSKGKREKVECRNHIRVIQPMNFNGQKLYVCGTNAHNPKDYVINANLTHLPRSQYVPGIGL- 233
             .|..|||..|.:|:|:|:::.|:  |...|..|||.|.:|....||.....|.|.:  .|..|
  Rat   103 QQCSFKGKDPKRDCQNYIKILLPL--NSSHLLTCGTAAFSPLCAYINTASFTLARDE--AGSVLL 163

  Fly   234 --GIGKCPYDPADNSTAVYVENGNPFGLPALYAGTNAEFTKADSVIFRSDLYNLTNGRKEANFKR 296
              |.|:||:||...|||:.|:.       .||.||.:.|...|..|.||            ...|
  Rat   164 EDGKGRCPFDPNFKSTALVVDG-------ELYTGTVSSFQGNDPAISRS------------QSPR 209

  Fly   297 TVKYDSKL--LDKPNFVGSFEIGEF----------VYFFFREHAVEYINCGKAVYSRVARVCKND 349
            ..|.:|.|  |..|.||.|..:.|.          :||||.|...|:......:.||||||||.|
  Rat   210 PTKTESSLNWLQDPAFVASAYVPESLGSPIGDDDKIYFFFSETGQEFEFFENTIVSRVARVCKGD 274

  Fly   350 RGGKYMISQNWATYLKARMNCSISSE-FPFYFNEIQSVYKM---PTD--DTKFYATFTT--NTNG 406
            .||:.::.|.|.::|||::.||...: ||  ||.:|.|:.:   |.|  .|.||..||:  :...
  Rat   275 EGGERVLQQRWTSFLKAQLLCSRPDDGFP--FNVLQDVFTLNPNPQDWRKTLFYGVFTSQWHRGT 337

  Fly   407 LIGSAVCSYDIRDINAAFDGKFKEQATSNSAWLPVLNSKVPEPRPGTC---------HNDTATLP 462
            ..|||:|.:.:.|:..||||.:|:.......|....: .||.||||.|         .|.:..||
  Rat   338 TEGSAICVFTMNDVQKAFDGLYKKVNRETQQWYTETH-PVPTPRPGACITNSARERKINSSLQLP 401

  Fly   463 DSVLNFIRKHPLMDKAVDHEFGNPVFFKRDVILTKLVVDKIRIDKLNQEFLVYFVATTSGHIYKI 527
            |.||||::.|.|||..|....   :..:......::.|.  |:..|:..:.|.|:.|..|.::|.
  Rat   402 DRVLNFLKDHFLMDGQVRSRL---LLLQPQARYQRVAVH--RVPGLHSTYDVLFLGTGDGRLHKA 461

  Fly   528 VQFMHYGQRHSNLVDIFEASPHSEPIREMTLSHKTGSLYVATDHQVKQIDIAMCARRYDSCFRCV 592
            |..    ....::::..:..|..:|::.:.|....|.||.::...|.|:.:|.|: .|.:|..|:
  Rat   462 VTL----DSRVHIIEELQVFPQGQPVQNLLLDSHGGLLYASSHSGVVQVPVANCS-LYPTCGDCL 521

  Fly   593 --SDPYCGWD-----------KDVNACRPYQLGLLQDV--ANETSGICDTSVLR----------K 632
              .||||.|:           .|: |.||:    :||:  || |..:|:.|..:          |
  Rat   522 LARDPYCAWNGSTCKLLSLYQPDL-ASRPW----IQDIEGAN-TKELCNISSAKARSFKPGKPCK 580

  Fly   633 KVTSSYGQTLHLSCFVKMPEVLRKKQTR-WYHHSTE-KGRYEVRYTPTKYIDTNEGGLVLLAVNE 695
            :|.........|:|     .:|....|| |.|:... ......|..|:       |.|:|:...:
  Rat   581 QVQIQANTVNTLAC-----PLLSNLATRLWLHNGAPVNASASCRVLPS-------GDLLLVGSQQ 633

  Fly   696 G----------DGGR--YDSYL---------------DGTLLCSYGVTVDAHRCSPPS-QKQDYQ 732
            |          :|.|  ..||.               |||     .|.::..|.|.|: .:..:.
  Rat   634 GLGVFQCWSIEEGFRQLVASYCPEVKEDGVMDQNDQSDGT-----PVIINTSRVSAPAGGRASWG 693

  Fly   733 KIYSHWCNEF-------------------EKYKSAMKQWQAKQEQCGLKDKTGP--ISSNGKHVN 776
            ...|:| |||                   .:.:..||.:..:.|...:..||.|  :....:.:|
  Rat   694 ADKSYW-NEFLVMCTLFGLAVVLLVLFFLYRNRDGMKLFLKQGECASVHPKTRPAVLPPETRPLN 757

  Fly   777 DVFSNDALVXPRKYLYSFEFLSSATEG----VASAHRKLYI---FVFVLTIFAR 823
            .|......:..|.|    :.||.::.|    ..|..|.|.|   ||.|..:..|
  Rat   758 GVGPPSTPLDHRGY----QALSDSSPGPRVFTESEKRPLSIQDSFVEVSPVCPR 807

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Sema2b	NP_001261024.1	Sema_2A	120..579	CDD:200499	150/502 (30%)
		PSI	583..>606	CDD:396154	9/35 (26%)
		Ig	632..724	CDD:472250	24/120 (20%)
		Ig strand B	642..646	CDD:409353	1/3 (33%)
		Ig strand C	658..662	CDD:409353	2/4 (50%)
		Ig strand E	686..690	CDD:409353	2/3 (67%)
		Ig strand F	700..705	CDD:409353	2/6 (33%)
		Ig strand G	717..720	CDD:409353	0/2 (0%)
Sema4b	NP_001163933.1	Sema	46..509	CDD:472829	151/508 (30%)
		PSI	510..556	CDD:396154	15/51 (29%)
		Ig_Sema4B_like	577..661	CDD:409456	19/95 (20%)
		Ig strand A	577..583	CDD:409456	1/5 (20%)
		Ig strand B	587..595	CDD:409456	2/12 (17%)
		Ig strand C	601..607	CDD:409456	3/5 (60%)
		Ig strand C'	609..612	CDD:409456	0/2 (0%)
		Ig strand D	618..622	CDD:409456	1/3 (33%)
		Ig strand E	624..630	CDD:409456	3/5 (60%)
		Ig strand F	636..645	CDD:409456	0/8 (0%)
		Ig strand G	648..661	CDD:409456	3/12 (25%)