Protein Alignment Hspa8 and Hsp70Bb

DIOPT Version :9

• Sequence 1: NP_077327.1
• Gene: Hspa8 / 24468
• RGDID: 621725
• Length: 646
• Species: Rattus norvegicus
• Sequence 2: NP_524927.2
• Gene: Hsp70Bb / 48582
• FlyBaseID: FBgn0013278
• Length: 641
• Species: Drosophila melanogaster
• Alignment Length: 643
• Identity: 483/643 - (75%)
• Similarity: 554/643 - (86%)
• Gaps: 4/643 - (0%)

  Rat     5 PAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTNTVFD 69
            ||:|||||||||||||:|||||||||||||||||||||||||:||||||.|||||||||.|||||
  Fly     2 PAIGIDLGTTYSCVGVYQHGKVEIIANDQGNRTTPSYVAFTDSERLIGDPAKNQVAMNPRNTVFD 66

  Rat    70 AKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPKVQVEYKGETKSFYPEEVSSMVLTKMKEIAEAY 134
            |||||||::||..:..|||||||.||:|.|:||:.||||||:|.|.|||:||||||||||.||||
  Fly    67 AKRLIGRKYDDPKIAEDMKHWPFKVVSDGGKPKIGVEYKGESKRFAPEEISSMVLTKMKETAEAY 131

  Rat   135 LGKTVTNAVVTVPAYFNDSQRQATKDAGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFD 199
            ||:::|:||:||||||||||||||||||.|||||||||||||||||:||||||.:..||||||||
  Fly   132 LGESITDAVITVPAYFNDSQRQATKDAGHIAGLNVLRIINEPTAAALAYGLDKNLKGERNVLIFD 196

  Rat   200 LGGGTFDVSILTIEDG-IFEVKSTAGDTHLGGEDFDNRMVNHFIAEFKRKHKKDISENKRAVRRL 263
            |||||||||||||::| :|||:||||||||||||||||:|.|...|||||:|||:..|.||:|||
  Fly   197 LGGGTFDVSILTIDEGSLFEVRSTAGDTHLGGEDFDNRLVTHLAEEFKRKYKKDLRSNPRALRRL 261

  Rat   264 RTACERAKRTLSSSTQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDK 328
            |||.|||||||||||:|:||||:|:||.||||.::|||||||.|||||.||.||||||.|||:||
  Fly   262 RTAAERAKRTLSSSTEATIEIDALFEGQDFYTKVSRARFEELCADLFRNTLQPVEKALNDAKMDK 326

  Rat   329 SQIHDIVLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLLL 393
            .||||||||||||||||:|.|||:||:||.||.||||||||||||||||||||||:|..:||:||
  Fly   327 GQIHDIVLVGGSTRIPKVQSLLQEFFHGKNLNLSINPDEAVAYGAAVQAAILSGDQSGKIQDVLL 391

  Rat   394 LDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGK 458
            :||.||||||||||||||.||:||..||.|||:||:|||||||||.|||||||||||||||.||.
  Fly   392 VDVAPLSLGIETAGGVMTKLIERNCRIPCKQTKTFSTYSDNQPGVSIQVYEGERAMTKDNNALGT 456

  Rat   459 FELTGIPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKGRLSKEDIERMVQEAE 523
            |:|:|||||||||||||||||:||||||||||.:.||||...|||.|||||||:.:|:|||.|||
  Fly   457 FDLSGIPPAPRGVPQIEVTFDLDANGILNVSAKEMSTGKAKNITIKNDKGRLSQAEIDRMVNEAE 521

  Rat   524 KYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQGKINDEDKQKILDKCNEIISWLDKNQTAE 588
            ||..||||.|.:::|:|:||||.||:|.:|| :...||:::.||..:||||||.|.|||.|.|||
  Fly   522 KYADEDEKHRQRITSRNALESYVFNVKQSVE-QAPAGKLDEADKNSVLDKCNETIRWLDSNTTAE 585

  Rat   589 KEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGGFPGGGAPPSGGASSGPTIEEVD 646
            ||||:|:.:||.:.|:||:||::|...|..|| ||...|..|...|| .||||:||||
  Fly   586 KEEFDHKMEELTRHCSPIMTKMHQQGAGAAGG-PGANCGQQAGGFGG-YSGPTVEEVD 641

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Hspa8	NP_077327.1	PTZ00009	1..613	CDD:240227	465/608 (76%)
		Nucleotide-binding domain (NBD). /evidence=ECO:0000250|UniProtKB:P11142	2..386		309/381 (81%)
		Interaction with BAG1. /evidence=ECO:0000250	186..377		150/191 (79%)
		Substrate-binding domain (SBD). /evidence=ECO:0000250|UniProtKB:P11142	394..509		92/114 (81%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	614..646		15/31 (48%)
Hsp70Bb	NP_524927.2	PTZ00009	2..641	CDD:240227	481/641 (75%)
		HSPA1-2_6-8-like_NBD	3..379	CDD:212675	304/375 (81%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0443
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG53894
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000049
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100083
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	6	5.720