DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment KIF4A and kif4

DIOPT Version :9

Sequence 1:	NP_036442.3	Gene:	KIF4A / 24137	HGNCID:	13339	Length:	1232	Species:	Homo sapiens
Sequence 2:	NP_957117.1	Gene:	kif4 / 393796	ZFINID:	ZDB-GENE-040426-1545	Length:	1248	Species:	Danio rerio

Alignment Length:	1265	Identity:	771/1265 - (60%)
Similarity:	963/1265 - (76%)	Gaps:	56/1265 - (4%)

- Green bases have known domain annotations that are detailed below.

Human     2 KEEVKGIPVRVALRCRPLVPKEISEGCQMCLSFVPGEPQVVVGTDKSFTYDFVFDPSTEQEEVFN 66
            ||:.|.|||||||||||||||||:||||.||:|||.||||:||.||:||:|:||||:||||||||
Zfish     3 KEDEKAIPVRVALRCRPLVPKEINEGCQCCLNFVPSEPQVIVGNDKAFTFDYVFDPTTEQEEVFN 67

Human    67 TAVAPLIKGVFKGYNATVLAYGQTGSGKTYSMGGAYTAEQENEPTVGVIPRVIQLLFKEIDKKSD 131
            |||:||:.|:||||:||||||||||||||:||||.||:|||||||||||||||:.:|:|..|::|
Zfish    68 TAVSPLLSGLFKGYHATVLAYGQTGSGKTFSMGGTYTSEQENEPTVGVIPRVIRRIFQEKAKRTD 132

Human   132 FEFTLKVSYLEIYNEEILDLLCPSREKAQINIREDPKEGIKIVGLTEKTVLVALDTVSCLEQGNN 196
            .||.|.||||||||||||||||.|::|..|:||||||:|||||||||:.||.|.:.|.|||.||:
Zfish   133 CEFVLAVSYLEIYNEEILDLLCTSKDKPVISIREDPKDGIKIVGLTERDVLNAHEMVCCLEMGNS 197

Human   197 SRTVASTAMNSQSSRSHAIFTISLEQRKKSDKNSSFRSKLHLVDLAGSERQKKTKAEGDRLKEGI 261
            :|||.|||||:.|||||||||||||||:..||.....||||||||||||||||||||||||||||
Zfish   198 ARTVGSTAMNAASSRSHAIFTISLEQRRNGDKTDVMVSKLHLVDLAGSERQKKTKAEGDRLKEGI 262

Human   262 NINRGLLCLGNVISALGDD-KKGGFVPYRDSKLTRLLQDSLGGNSHTLMIACVSPADSNLEETLN 325
            :||||||.||||||||||: |||.|||||||||||||||||||||||||||||||||||:|||:|
Zfish   263 SINRGLLSLGNVISALGDESKKGSFVPYRDSKLTRLLQDSLGGNSHTLMIACVSPADSNIEETIN 327

Human   326 TLRYADRARKIKNKPIVNIDPQTAELNHLKQQVQQLQVLLLQAHGGTLPGSITVEPSENLQSLME 390
            |||||||||||||||::|:||:.||:..||||||:|||:||.|.||..|.....||:||:..::|
Zfish   328 TLRYADRARKIKNKPVLNVDPRAAEMKRLKQQVQELQVMLLHARGGVAPVLSGSEPAENVSKVLE 392

Human   391 KNQSLVEENEKLSRGLSEAAGQTAQMLERIILTEQANEKMNAKLEELRQHAACKLDLQKLVETLE 455
            :|:||.::|.||||.|||:.||||.|.||:::.||.|||:.:||:|||||||||:|||:||||||
Zfish   393 QNRSLQDQNSKLSRELSESVGQTAAMFERMLMMEQTNEKLQSKLDELRQHAACKVDLQRLVETLE 457

Human   456 DQELKENVEIICNLQQLITQLSDETVACMAAAID-------TAVEQEAQVETS------------ 501
            ||||:||||:|.||||:|.:|..|: |.:||.||       :::|..|....|
Zfish   458 DQELRENVEVIQNLQQIILELQRES-AGIAATIDAITSGNASSLEAAAHCSPSTAGAQAEICPGQ 521

Human   502 ----PETSRSSDAFTTQHALRQAQMSKELVELNKALALKEALARKMTQNDSQLQPIQYQYQDNIK 562
                ||...|.:|:|.|||||||||||||:||||.||||||..:||.||||.|:|:|.|||::|:
Zfish   522 DGAVPEAKDSPEAYTAQHALRQAQMSKELIELNKVLALKEAFVKKMCQNDSHLEPMQTQYQESIQ 586

Human   563 ELELEVINLQKEKEELVLELQTAKKDANQAKLSERRRKRLQELEGQIADLKKKLNEQSKLLKLKE 627
            .|:..|.:||||||:||..||:||||.|||||||:|||||||||||:.:|||||.:|||||||||
Zfish   587 SLQAAVGSLQKEKEDLVQTLQSAKKDTNQAKLSEQRRKRLQELEGQMTELKKKLQDQSKLLKLKE 651

Human   628 STERTVSKLNQEIRMMKNQRVQLMRQMKEDAEKFRQWKQKKDKEVIQLKERDRKRQYELLKLERN 692
            |:.|.|:||||||:.||:||||||||||:|:||||.|||||||||:||||:|||||||:|||||:
Zfish   652 SSVRNVAKLNQEIQAMKSQRVQLMRQMKDDSEKFRLWKQKKDKEVLQLKEKDRKRQYEMLKLERD 716

Human   693 FQKQSNVLRRKTEEAAAANKRLKDALQKQREVADKRKETQSRGMEGTAARVKNWLGNEIEVMVST 757
            ||||::||||||||||||||||||||||:.|||:|||:.|:||.|....||||||.|||||:|||
Zfish   717 FQKQASVLRRKTEEAAAANKRLKDALQKRSEVAEKRKDVQNRGAESVTNRVKNWLLNEIEVLVST 781

Human   758 EEAKRHLNDLLEDRKILAQDVAQLKEKKESGENPPPKLRRRTFSLTEVRGQVSESEDSITKQIES 822
            |||:|||.|||||||:||::::||:::.|:||.|..|:||||.:::|:.|: .|.|.||:||:||
Zfish   782 EEARRHLQDLLEDRKMLAEEISQLRQQMEAGEKPVSKVRRRTLTISELEGK-CELEASISKQVES 845

Human   823 LETEMEFRSAQIADLQQKLLDAESEDRPKQRWENIATILEAKCALKYLIGELVSSKIQVSKLESS 887
            |:|||..|||||||||||:|||::|.|.||||::|.||:|||.|||.|:.|:|:||...:|.||.
Zfish   846 LDTEMALRSAQIADLQQKVLDADNEGRMKQRWDSITTIIEAKSALKILMAEVVASKTLNAKQESE 910

Human   888 LKQSKTSCADMQKMLFEERNHFAEIETELQAELVRMEQQHQEKVLYLLSQLQQSQMAEKQLEESV 952
            |:|.|.:..||||:|.:||...:.::.|.|:.||.|||:|||||||||.|||...:|.::.:|.|
Zfish   911 LQQEKANLLDMQKVLCDERKLMSAMDMEHQSHLVEMEQRHQEKVLYLLGQLQNKPVALEEQQELV 975

Human   953 SEKEQQLLSTLKCQDEELEKMREVCEQNQQLLRENEIIKQKLTLLQVASRQKHLPKDTLLSPDSS 1017
            |::||:||..|:.|:||:||:||:.:|||:|..|||..||||.|.|:.|.:|.| .....|||:|
Zfish   976 SKREQELLQRLQFQEEEIEKLRELSDQNQKLKEENEQYKQKLHLAQLTSAKKVL-SAVNESPDTS 1039

Human  1018 FEYVPPKPK-----PSRVKEKFLEQSMDIEDLKYCSEHSVNEHEDGDGDDDEGDDEEWKPTKLVK 1077
            |||:|||||     .:|.::   ::.:|:::|...|             |.|.:::||:|.|..|
Zfish  1040 FEYIPPKPKAKMHSTARARQ---QEPIDLDELMSPS-------------DSEKEEDEWRPEKAEK 1088

Human  1078 VSR----KNIQGCSCKGWCGNKQCGCRKQKSDCGVDCCCDPTKCRNRQQGKDSLGTVERTQDSEG 1138
            ..|    ..:.||:|:|.|.||.|.|||.|..||.:|.||..|||| .:.:.|:...|.|...:.
Zfish  1089 TRRGSKKAKLNGCACRGRCVNKLCRCRKGKMTCGENCLCDHEKCRN-MENRSSVDLNETTDVFKD 1152

Human  1139 SFKL-EDPTEV-TPGLSFFNPVCATPNSKILKEMCDVEQVLSKKTPPAPSPFDLPELKHVATEYQ 1201
            |..| ||||.| :...:||.|.|..|..|.:.|:..:...|..:..|.|:..:.......:....
Zfish  1153 SSVLPEDPTAVGSRDATFFTPPCVPPTKKEIGEVWQLPADLKLEKKPVPAENERESDSEDSEGMN 1217

Human  1202 ENKAPGKKKKRALAS-NTSFFSGCSPIEEE 1230
            ...:..::.||.|:: ..||||||:||.||
Zfish  1218 NTSSFLRRNKRGLSNFKNSFFSGCTPIREE 1247

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
KIF4A	NP_036442.3	KISc_KIF4	8..337	CDD:276823	265/329 (81%)
		SMC_prok_B	<375..714	CDD:274008	230/361 (64%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	496..515		7/34 (21%)
		Smc	558..>918	CDD:224117	239/359 (67%)
		Required for the interaction with PRC1. /evidence=ECO:0000269\|PubMed:15297875	663..1232		291/580 (50%)
		Nuclear localization signal. /evidence=ECO:0000269\|PubMed:11736643	793..798		2/4 (50%)
		Globular	1000..1232		81/243 (33%)
		Pre-SET	<1078..1120	CDD:368254	21/45 (47%)
		CRD, required for [4Fe-4S] cluster binding and localization to the spindle midzone and midbody during anaphase and telophase. /evidence=ECO:0000269\|PubMed:29848660	1086..1144		25/58 (43%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1122..1142		4/19 (21%)
kif4	NP_957117.1	KISc_KIF4	9..339	CDD:276823	265/329 (81%)
		KISc	10..345	CDD:214526	269/334 (81%)
		BST2	568..644	CDD:293321	50/75 (67%)
		DUF3435	<938..1082	CDD:288746	70/160 (44%)
		TCR	1100..1136	CDD:281618	21/36 (58%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C194324495
Domainoid	1	1.000	911	1.000	Domainoid score	I564
eggNOG	1	0.900	-	-		E2759_KOG0244
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H69022
Inparanoid	1	1.050	1444	1.000	Inparanoid score	I1046
NCBI	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D35421at7742
OrthoFinder	1	1.000	-	-		FOG0003140
OrthoInspector	1	1.000	-	-		otm27675
orthoMCL	1	0.900	-	-		OOG6_100464
Panther	1	1.100	-	-	LDO	PTHR47969
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3568
SonicParanoid	1	1.000	-	-		X2108
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	15	14.790