Protein Alignment KIF4A and Khc-73

DIOPT Version :10

• Sequence 1: NP_036442.3
• Gene: KIF4A / 24137
• HGNCID: 13339
• Length: 1232
• Species: Homo sapiens
• Sequence 2: NP_001261000.1
• Gene: Khc-73 / 36718
• FlyBaseID: FBgn0019968
• Length: 1957
• Species: Drosophila melanogaster
• Alignment Length: 1255
• Identity: 325/1255 - (25%)
• Similarity: 539/1255 - (42%)
• Gaps: 286/1255 - (22%)

Human    10 VRVALRCRPLVPKEIS---------EGCQMCLSFVPGEPQVVVGTDKSFTYDFVF---DPSTE-- 60
            ::||:|.||...:||.         |..|..|...|...::.....|:|.:|..|   :|..|
  Fly     6 IKVAVRVRPFNRREIELDTKCIVEMEKQQTILQNPPPLEKIERKQPKTFAFDHCFYSLNPEDENF 70

Human    61 --QEEVFNTAVAPLIKGVFKGYNATVLAYGQTGSGKTYSMGGAYTAEQENEPTVGVIPRVIQLLF 123
              ||.||:.....::...|:||||.:.|||||||||:|:|.|.    ||::   |:|||:...||
  Fly    71 ASQETVFDCVGRGILDNAFQGYNACIFAYGQTGSGKSYTMMGT----QESK---GIIPRLCDQLF 128

Human   124 KEIDKKS--DFEFTLKVSYLEIYNEEILDLLCPSREKAQINIREDPKEGIKIVGLTEKTVLVALD 186
            ..|..||  :..:.::|||:|||||::.|||.|...|..:.:||....|..:.||::..|....|
  Fly   129 SAIANKSTPELMYKVEVSYMEIYNEKVHDLLDPKPNKQSLKVREHNVMGPYVDGLSQLAVTSYQD 193

Human   187 TVSCLEQGNNSRTVASTAMNSQSSRSHAIFTISLEQ---RKKSDKNSSFRSKLHLVDLAGSERQK 248
            ..:.:.:||.|||||:|.||::||||||:|::.|.|   .:.:..:....|::.|||||||||..
  Fly   194 IDNLMTEGNKSRTVAATNMNAESSRSHAVFSVVLTQILTDQATGVSGEKVSRMSLVDLAGSERAV 258

Human   249 KTKAEGDRLKEGININRGLLCLGNVISALGDD---KKGG---FVPYRDSKLTRLLQDSLGGNSHT 307
            ||.|.|||||||.|||:.|..||.|||.|.|.   ||.|   |||||||.||.||:|:|||||.|
  Fly   259 KTGAVGDRLKEGSNINKSLTTLGLVISKLADQSNGKKSGNDKFVPYRDSVLTWLLKDNLGGNSRT 323

Human   308 LMIACVSPADSNLEETLNTLRYADRARKIKNKPIVNIDPQTAELNHLKQQVQQLQVLLLQAHGGT 372
            :|:|.:||:..|.||||:||||||||::|.|..:||.||....:..|:.:|:.|:.:|..|.|..
  Fly   324 VMVATISPSADNYEETLSTLRYADRAKRIVNHAVVNEDPNARIIRELRHEVETLRSMLKHATGSP 388

Human   373 LPGSITVEPSENLQSLMEKNQSLVEENEKLSRGLSEAAGQTAQMLERIILTEQANEKMNAKLEEL 437
            : |.:               |..:.|:|.|.:.:|    ||.:  |:::.||:...:....||::
  Fly   389 V-GDV---------------QDKLAESENLMKQIS----QTWE--EKLVKTERIQNERQQALEKM 431

Human   438 R---QHAACKLDLQK--LVETLEDQELKENVEIICNLQQLITQLSDETVA--------------- 482
            .   |.:..|::..|  ||....|..|.|         .|:..|.|.|:.
  Fly   432 GISVQASGIKVEKNKYYLVNLNADPSLNE---------LLVYYLKDRTLIGGRTISGQQPDIQLS 487

Human   483 --------CMAAAIDTAVEQE------------AQVETSPETSRSSDAFTTQHALRQAQMSKELV 527
                    |:....|:.:..|            |.||.:|..:.....:...|..|
  Fly   488 GLGIQPEHCVITIEDSGLYMEPVQGARCFVNGSAAVEKTPLQNGDRILWGNHHFFR--------- 543

Human   528 ELNKALALKEALARKMTQNDSQLQPIQYQY-QDNIKELELEVINLQKEKEELVLELQTAKKDANQ 591
             :|...:...::.....|..:||  |.|.: :|.|.:.||....:|    ..:..|:...::..|
  Fly   544 -VNSPKSNNTSMCASEPQTPAQL--IDYNFARDEIMQNELSNDPIQ----TAIARLERQHEEDKQ 601

Human   592 AKLSERRRKRLQELEGQIADLKKKLNEQSKL--------LKLKESTERT------VSKLNQE--- 639
            ..|.::|    ||.|.|...|:..|:..:..        |::.:.|..|      |.|..||
  Fly   602 VALEKQR----QEYERQFQQLRNILSPSTPYAPYAPYDPLRMGKITPNTPTSQMRVEKWAQERDE 662

Human   640 --IRMMKNQRVQLMR--QMKEDAEKFRQWKQKKDKEVIQLK------ERDRKRQYELLKLERNFQ 694
              .|.:...:..:||  .:.::|....:..:||.|..:.|:      ..:|:|        ..|.
  Fly   663 MFRRSLGQLKTDIMRANSLVQEANFLAEEMEKKTKFSVTLQIPPANLSPNRRR--------GAFV 719

Human   695 KQSNVLRRKTEEAAA--ANKRLKDALQKQREVADKRKETQSRGMEGTAARVKN--WLGNEIEVMV 755
            .:..:|.::|...:.  ..::|::.|...||:..:.||....|::...|:.::  :...|...::
  Fly   720 SEPAILVKRTNSGSQIWTMEKLENKLIDMREMYQEHKERVLNGLDEDNAKPQDPFYESQENHNLI 784

Human   756 STEEAKRHLNDLLEDRK------ILAQ--DVA---QLKEKKESGENPPPKLRRRTFSLTEVRGQV 809
            ..  |...|..|..|.|      |::|  :||   |::.::.:|:.|..::..   |::|..|..
  Fly   785 GV--ANIFLEVLFHDVKLDYHTPIISQQGEVAGRLQVEIERIAGQMPQDRMCE---SVSESSGDS 844

Human   810 -SESEDSITKQIESLETEMEFRSAQIADLQQK------------------LLDAESEDRPKQ--- 852
             .|.:|.:......:...:..:.|....|...                  :::|||....:.
  Fly   845 RDEYDDPVDPTSNQITCRVTIKCASGLPLSLSNFVFCQYTFWGHQEMVVPVINAESTAHDQNMVF 909

Human   853 RWEN----IATILEAKCALKYLIGELVSSKIQVSKLESSLKQSKTSCADMQKMLFEER---NHFA 910
            ::|:    ..||.|.  .|::.|...:|.::...:   |...|||...::::...:.|   :.:|
  Fly   910 KFEHTQDFTVTINEE--FLEHCIEGALSIEVWGHR---SAGFSKTKGWEVEQQQAKARSLVDRWA 969

Human   911 EIETELQ-----------AELVRMEQQHQEKVL----YLLSQLQQSQ--MAEKQLEES------- 951
            |:..:::           .|...:|..::.:||    |.|.|.||.:  :..|.::.|
  Fly   970 ELSRKIELWVEIHELNDNGEYSPVEVTNRNEVLTGGIYQLRQGQQRRVNVRVKPVQNSGTLPIIC 1034

Human   952 ------------VSEKEQQLLSTLKCQDEELEKMREVCEQNQQLLRENEIIKQKLTLLQVASRQK 1004
                        |..:.|:.|.:.  |:|:|..:||  :.::.|.|..:.:.|::.:|.....:.
  Fly  1035 QSIVNVAIGSVTVRSRLQRPLDSY--QEEDLTVLRE--KWSEALGRRRQYLDQQIQMLIKKEEKN 1095

Human  1005 HLPKDTLLS------------------------PDSSFEYVPPKPKPSRVKEKFLEQSMD----- 1040
            ...::..||                        |.:...:.||......|...||..:.|
  Fly  1096 EQERERELSLVHQWVSLTEERNAVLVPAPGSGIPGAPASWEPPSGMEPHVPVLFLNLNGDDLSAQ 1160

Human  1041 --IEDLKYCSEHSVNEHEDG 1058
              .::|.....:|:...|.|
  Fly  1161 NTNDELSVAGINSILSKEHG 1180

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
KIF4A	NP_036442.3	KISc_KIF4	8..337	CDD:276823	157/353 (44%)
		PRK11281	383..>646	CDD:481316	62/322 (19%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	496..515		4/18 (22%)
		Smc	<517..>1033	CDD:440809	116/647 (18%)
		Required for the interaction with PRC1. /evidence=ECO:0000269|PubMed:15297875	663..1232		90/513 (18%)
		Nuclear localization signal. /evidence=ECO:0000269|PubMed:11736643	793..798		0/4 (0%)
		Globular	1000..1232		13/90 (14%)
		CRD, required for [4Fe-4S] cluster binding and localization to the spindle midzone and midbody during anaphase and telophase. /evidence=ECO:0000269|PubMed:29848660	1086..1144
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1122..1142
Khc-73	NP_001261000.1	KISc_KIF1A_KIF1B	4..359	CDD:276816	159/359 (44%)
		Kinesin_assoc	356..468	CDD:465047	33/142 (23%)
		FHA_KIF13	446..547	CDD:438758	20/119 (17%)
		KIF1B	741..786	CDD:463574	9/44 (20%)
		DUF3694	989..1243	CDD:463599	36/196 (18%)
		CAP_GLY	1785..1850	CDD:460154