DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment KIF4A and Klp3A

DIOPT Version :9

Sequence 1:	NP_036442.3	Gene:	KIF4A / 24137	HGNCID:	13339	Length:	1232	Species:	Homo sapiens
Sequence 2:	NP_001284830.1	Gene:	Klp3A / 31240	FlyBaseID:	FBgn0011606	Length:	1212	Species:	Drosophila melanogaster

Alignment Length:	1319	Identity:	456/1319 - (34%)
Similarity:	678/1319 - (51%)	Gaps:	222/1319 - (16%)

- Green bases have known domain annotations that are detailed below.

Human    10 VRVALRCRPLVPKEISEGCQMCLS-FVPGEPQVVVGTDKSFTYDFVFDPSTEQEEVFNTAVAPLI 73
            |.||||.||||..|:..||::.:. ...|.|||.|..::|:||::|||....|:::|.|.|...:
  Fly     9 VAVALRVRPLVQSELDRGCRIAVERSADGAPQVTVNRNESYTYNYVFDIDDSQKDLFETCVQAKV 73

Human    74 KGVFKGYNATVLAYGQTGSGKTYSMGGAYTAEQENEPTVGVIPRVIQLLFKEI-DKKSDFEFTLK 137
            |.:..|||.|:||||||||||||:||.|:....::.  ||||||.:..:|..| :.:|:|.|.:.
  Fly    74 KKLLNGYNVTILAYGQTGSGKTYTMGTAFNGVLDDH--VGVIPRAVHDIFTAIAEMQSEFRFAVT 136

Human   138 VSYLEIYNEEILDLL-CPSREKAQINIREDPKEGIKIVGLTEKTVLVALDTVSCLEQGNNSRTVA 201
            .|::|:|.|:..||. ..:|:||.::||| .|..|.:.||||..|..|......|.:|:..|.||
  Fly   137 CSFVELYQEQFYDLFSSKTRDKATVDIRE-VKNRIIMPGLTELVVTSAQQVTDHLIRGSAGRAVA 200

Human   202 STAMNSQSSRSHAIFTISLEQRKKSDKNSSFRSKLHLVDLAGSERQKKTKAEGDRLKEGININRG 266
            :||||..|||||||||::|...|...|.|...|:.:|||||||||..||.|.|||.|||:|||:|
  Fly   201 ATAMNETSSRSHAIFTLTLVATKLDGKQSVTTSRFNLVDLAGSERCSKTLASGDRFKEGVNINKG 265

Human   267 LLCLGNVISALGDDKKGGFVPYRDSKLTRLLQDSLGGNSHTLMIACVSPADSNLEETLNTLRYAD 331
            ||.|||||:|||..:..|::|||.|||||||||||||||.|||||||||||.|:.|||:||||||
  Fly   266 LLALGNVINALGSGQAAGYIPYRQSKLTRLLQDSLGGNSITLMIACVSPADYNVAETLSTLRYAD 330

Human   332 RARKIKNKPIVNIDPQTAELNHLKQQVQQLQVLLLQAHGGTLP------------GSITVEPS-- 382
            ||.:|||||:||:||..||:|.||..:|:|:|.||.  ||.:.            |:|..|.|
  Fly   331 RALQIKNKPVVNLDPHAAEVNMLKDVIQKLRVELLS--GGKMSSSLISAVGAAGLGAIPCEESLA 393

Human   383 ------ENLQSLMEKNQSLVEENEKLSRGLSEAAGQTAQMLERIILTEQANEKMNAKLEELRQHA 441
                  ..:|.|.|:.::|.:.|.||.:.|.::.....:...|..:.|||::|:.:.:.||:.
  Fly   394 GSMANAAEIQRLKEQVRTLQDRNRKLQQELHQSLLDLTEKEMRAHIAEQAHDKLRSHVSELKN-- 456

Human   442 ACKLDLQKLVE-----TLEDQELKE--------NVEIICNLQQLITQLSDETVACMAAAIDTAVE 493
              |||.::..:     |..|.|:::        :||:....::|.:| ..|:...:::...|...
  Fly   457 --KLDQREQAQFGNENTNGDNEMRDFSLLVNRVHVELQRTQEELESQ-GHESRQRLSSRSHTEGG 518

Human   494 QEAQVETSPETSRSSDAFTTQHALRQAQMSKELVELNKALALKEALARKMTQNDSQLQPIQYQYQ 558
            :....|........|:.:|.    :|...:.||..:|:.|.||:.|..::.:|.|:|..     .
  Fly   519 ESGGDEVHEMLHSHSEEYTN----KQMNFAGELRNINRQLDLKQELHERIMRNFSRLDS-----D 574

Human   559 DNIKELEL---EVINLQKEKEELVLELQTAKKDANQAKLSERRRKRLQELEGQIADLKKKLNEQS 620
            |...:|.|   ::.:|:.|:.:|:.:|:..|.....|||:|.||||||.||.:|:||::||..|:
  Fly   575 DEDVKLRLCNQKIDDLEAERRDLMDQLRNIKSKDISAKLAEERRKRLQLLEQEISDLRRKLITQA 639

Human   621 KLLKLKESTERTVSKLNQEIRMMKNQRVQLMRQMKEDAEKFRQWKQKKDKEVIQLKERDRKRQYE 685
            .|||:::.....:..|:.|||.||..:|:|:|.|:.::|||||||..::||:.|||.:|||.|.|
  Fly   640 NLLKIRDKEREKIQNLSTEIRTMKESKVKLIRAMRGESEKFRQWKMVREKELTQLKSKDRKMQSE 704

Human   686 LLKLERNFQKQSNVLRRKTEEAAAANKRLKDALQKQREVADKRKETQSRGMEGTA-------ARV 743
            :::.:....||..||:||.|||.||||||||||::|.....:|.:.:..|  |:|       |:.
  Fly   705 IVRQQTLHSKQRQVLKRKCEEALAANKRLKDALERQASAQAQRHKYKDNG--GSAAGSSNANAKT 767

Human   744 KNWLGNEIEVMVSTEEAKRHLNDLLEDRKILAQDVAQLKEKKESGENPPPKLRRRTFSLTEVRGQ 808
            .:|:..|:|:::|..:|:..|..|:|||.::......|:::|.|  :|
  Fly   768 DSWVDRELEIILSLIDAEHSLEQLMEDRAVINNHYHLLQQEKTS--DP----------------- 813

Human   809 VSESEDSITKQIESLETEMEFRSAQIADLQQKLLDAESEDRPKQRWENIATILEAKCALKYLIGE 873
             :|:.:. .:.:.|||.|:|.|:|||:|||||:...:.:.|.:...|.:.::.|::...|.|:..
  Fly   814 -AEAAEQ-ARILASLEEELEMRNAQISDLQQKVCPTDLDSRIRSLAEGVQSLGESRTVSKQLLKT 876

Human   874 LVSSKIQVSKLE-SSLKQSKTSCADMQKMLFEERNHFAEIETELQAELVRMEQQHQ--EKVLYLL 935
            ||    |..:|: |||.:.:|:.                  .||:|:|:..:||..  .|.|.||
  Fly   877 LV----QQRRLQASSLNEQRTTL------------------DELRAQLLDAQQQEDAASKRLRLL 919

Human   936 SQLQQSQMAEKQLEESVSEKEQQLLSTLKCQDEELEKMREVCEQNQ---QLLRENEIIKQKLTLL 997
            ....:.||..:|  .:..||...|:.|...:..|.....|..::||   :||...|.::|:|..|
  Fly   920 QSQHEEQMLAQQ--RAYEEKVSVLIRTANQRWAEARSPAEDQQRNQILEELLSSREALQQELDKL 982

Human   998 QVASRQKHL-----PKDTLLSPDSSFEYV-------------PPKPKPSRVKEKFLE-------- 1036
            :..::.|..     |:|.    |.||:.|             .|...||..|.|.::
  Fly   983 RAKNKSKSKAVKSEPQDL----DDSFQIVDGNETVVLSDVSDDPDWVPSTSKSKRIQSDSRNVIS 1043

Human  1037 ----QSMDIEDLKYCSEHSVNE---HEDGDGDDDEGDDEEWKPTKLVKVSRKNIQGCSCKGWCGN 1094
                |..::..|...|..|:|.   .|||                      |..:||.|:..|..
  Fly  1044 PPEKQDANVTSLGNSSIQSLNSTSATEDG----------------------KRCKGCKCRTKCTT 1086

Human  1095 KQCGCRKQKSDCGVDCCCDPTKCRN-------RQQGKDSLGTVERTQDSEGSFKLEDPTEVTPGL 1152
            |:|||....:.|...|.| .:.|||       ..|..|..|..:.|:|::   |.:|..:..|..
  Fly  1087 KRCGCLSGNNACSETCVC-KSNCRNPLNLKDHASQCGDGDGQKDETEDAD---KSDDDGDDEPQT 1147

Human  1153 SFFNPVCATPNSKILKEMCDVEQVLSKKTPPAP-----SPFD-LPELKHVATE------YQENKA 1205
            |..|.|      |.:             ||.||     ||.. |.|.|..||.      .::...
  Fly  1148 SKENAV------KFV-------------TPEAPGKVVASPKQTLQEPKAAATPLMNSNVVEDING 1193

Human  1206 PGKKKKRALASNT---SFF 1221
            |...|...||.:|   .||
  Fly  1194 PKLAKMSGLAFDTPKRKFF 1212

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
KIF4A	NP_036442.3	KISc_KIF4	8..337	CDD:276823	175/329 (53%)
		SMC_prok_B	<375..714	CDD:274008	114/362 (31%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	496..515		3/18 (17%)
		Smc	558..>918	CDD:224117	125/370 (34%)
		Required for the interaction with PRC1. /evidence=ECO:0000269\|PubMed:15297875	663..1232		172/627 (27%)
		Nuclear localization signal. /evidence=ECO:0000269\|PubMed:11736643	793..798		0/4 (0%)
		Globular	1000..1232		65/277 (23%)
		Pre-SET	<1078..1120	CDD:368254	15/48 (31%)
		CRD, required for [4Fe-4S] cluster binding and localization to the spindle midzone and midbody during anaphase and telophase. /evidence=ECO:0000269\|PubMed:29848660	1086..1144		19/64 (30%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1122..1142		5/19 (26%)
Klp3A	NP_001284830.1	KISc_KIF4	7..336	CDD:276823	175/329 (53%)
		Kinesin	14..335	CDD:278646	171/323 (53%)
		Smc	<396..>994	CDD:224117	192/658 (29%)
		FlaC	522..633	CDD:225888	37/119 (31%)
		TCR	1075..1110	CDD:281618	12/35 (34%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	313	1.000	Domainoid score	I1299
eggNOG	1	0.900	-	-		E2759_KOG0244
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H69022
Inparanoid	1	1.050	605	1.000	Inparanoid score	I942
Isobase	1	0.950	-	0	Normalized mean entropy	S3090
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D369179at2759
OrthoFinder	1	1.000	-	-		FOG0003140
OrthoInspector	1	1.000	-	-		otm41233
orthoMCL	1	0.900	-	-		OOG6_100464
Panther	1	1.100	-	-	LDO	PTHR47969
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3568
SonicParanoid	1	1.000	-	-		X2108
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	14	13.850