Protein Alignment KIF4A and klp3

DIOPT Version :10

• Sequence 1: NP_036442.3
• Gene: KIF4A / 24137
• HGNCID: 13339
• Length: 1232
• Species: Homo sapiens
• Sequence 2: NP_594686.1
• Gene: klp3 / 2542195
• PomBaseID: SPAC1834.07
• Length: 554
• Species: Schizosaccharomyces pombe
• Alignment Length: 597
• Identity: 195/597 - (32%)
• Similarity: 299/597 - (50%)
• Gaps: 101/597 - (16%)

Human    10 VRVALRCRPLVPKEISEGCQMCLSFVPGEPQVVVGTDK---SFTYDFVFDPSTEQEEVFNTAVAP 71
            ::|..|.||....|...|....: :...:..|.:.|..   :|.:|.||.||:.|.::|:.::..
pombe     4 IKVVCRIRPTNQLEQDLGGNNVI-YPLNDSTVHIETSDYSGNFVFDRVFHPSSTQNDIFSYSIES 67

Human    72 LIKGVFKGYNATVLAYGQTGSGKTYSMGGAYTAEQENEPTVGVIPRVIQLLFKEI-DKKSDFEFT 135
            .:..:|.|||.||||||||||||||:|.|.    :.|....|:.||:::.:|.:| |..|..|:.
pombe    68 TVDDLFLGYNGTVLAYGQTGSGKTYTMMGI----ENNFEKEGMTPRMLRRIFDKIRDSPSTTEYE 128

Human   136 LKVSYLEIYNEEILDLLCPSREKAQINIREDPKEGIKIVGLTEKTVLVALDT--VSCLEQGNNSR 198
            :||||:|||.|:|.|||  |.:..::.:.||..:|:.:.||  ||:.|:.:|  :..|.:|..||
pombe   129 VKVSYMEIYMEKIHDLL--SEKNDRLTVHEDKLQGVYVQGL--KTIYVSSETEALDILNKGMGSR 189

Human   199 TVASTAMNSQSSRSHAIFTISLEQRKKSDKNSSFRSKLHLVDLAGSERQKKTKAEGDRLKEGINI 263
            .||||:||:||||||:||.:.:.| ..::...:.|.:|.||||||||...|:.|.|..|:|...|
pombe   190 AVASTSMNAQSSRSHSIFVLEVVQ-TDTESGETRRGRLFLVDLAGSESVGKSGAVGQTLEEAKKI 253

Human   264 NRGLLCLGNVISALGDDKKGGFVPYRDSKLTRLLQDSLGGNSHTLMIACVSPADSNLEETLNTLR 328
            ||.|..||.||::|.|.|. ..||||||||||:|::||||||.|.:|...||...|..|||:|||
pombe   254 NRSLSTLGMVINSLTDSKL-SHVPYRDSKLTRILKESLGGNSRTTLIINCSPDSYNATETLSTLR 317

Human   329 YADRARKIKNKPIVNIDPQTAELNHLKQQVQQLQVLLLQ---------------AHGGTLPGSIT 378
            :..||:.||||.:||.:   ..::.:|:|:...:..|.:               .|.....|
pombe   318 FGHRAKSIKNKAVVNSE---LSVDEMKRQLYIYKDALSRCVCGARINNNLDYNNCHSNVWSG--- 376

Human   379 VEPSENLQSLMEKNQSLVEENEKLSRGLSEA----AGQTAQMLERIILTEQANEKMNAKLEELRQ 439
             |.|..|.:|.||:            .|.||    ..:|.|        |..|::..:.:..:.:
pombe   377 -EHSLTLSNLAEKS------------NLKEAEIIQGNRTIQ--------ESNNDRDESTVASIHR 420

Human   440 HAACKLDLQKLVETLEDQELKENVEIICNLQQLITQLSDETVACMAAAIDTAVEQEAQVE----- 499
            |......:.:|....: .|||:...::.:.:|   ||||     :..|:..|  ||..||
pombe   421 HNFDSDSINRLYAEAQ-LELKQRDGVLSSTKQ---QLSD-----LMTALGDA--QERYVELVKNH 474

Human   500 ------TSPETSRSSDAFTTQHALRQAQMSKE-------LVELNKALA---------LKEALARK 542
                  |:..:......||.:...:...::.|       |..|:.:||         :|..::::
pombe   475 RVNSNLTANNSLNDKPGFTIEQKDKNFSINNERNNFLQKLSTLDSSLAALVNVQRKLIKALISKE 539

Human   543 MTQNDSQLQPIQ 554
            ..||.:.::.||
pombe   540 RPQNGTVIKKIQ 551

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
KIF4A	NP_036442.3	KISc_KIF4	8..337	CDD:276823	142/332 (43%)
		PRK11281	383..>646	CDD:481316	40/203 (20%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	496..515		5/29 (17%)
		Smc	<517..>1033	CDD:440809	10/54 (19%)
		Required for the interaction with PRC1. /evidence=ECO:0000269|PubMed:15297875	663..1232
		Nuclear localization signal. /evidence=ECO:0000269|PubMed:11736643	793..798
		Globular	1000..1232
		CRD, required for [4Fe-4S] cluster binding and localization to the spindle midzone and midbody during anaphase and telophase. /evidence=ECO:0000269|PubMed:29848660	1086..1144
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1122..1142
klp3	NP_594686.1	KISc_KHC_KIF5	1..325	CDD:276820	142/331 (43%)