Protein Alignment AP4E1 and AP-2alpha

DIOPT Version :10

• Sequence 1: NP_031373.2
• Gene: AP4E1 / 23431
• HGNCID: 573
• Length: 1137
• Species: Homo sapiens
• Sequence 2: NP_995607.1
• Gene: AP-2alpha / 33211
• FlyBaseID: FBgn0264855
• Length: 952
• Species: Drosophila melanogaster
• Alignment Length: 1156
• Identity: 243/1156 - (21%)
• Similarity: 460/1156 - (39%)
• Gaps: 320/1156 - (27%)

Human    48 SKHEEEKLIQQELSSLKATVSAPTTTLKMMKECMV-RLIYCEMLGYDASFGYIHAIKLAQQGNLL 111
            ||..|.|.|.:||:::::......|.....|:..| :|::..:||:|..||::.|:.|.......
  Fly    36 SKEAEVKRINKELANIRSKFKGDKTLDGYQKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKYS 100

Human   112 EKRVGYLAVSLFLHESHELLLLLVNTVVKDLQSTNLVEVCMALTVVSQIFPCEM-------IPAV 169
            ||::|||.:|:.::.:.:|:.|::.::..||||.|.|.|.:||..::.|...:|       ||.:
  Fly   101 EKQIGYLFISVLVNTNSDLIRLIIQSIKNDLQSRNPVHVNLALQCIANIGSRDMAESFSNEIPKL 165

Human   170 LPLIEDKLQHSKEIVRRKAVLALYKFHLIAPNQV-------QHIHIKFRKALCDRDVGVMAASLH 227
            | :..|.:    ::|::.|.|.|.:....:|:.:       :.||:     |.|:.:||:.|:..
  Fly   166 L-VSGDTM----DVVKQSAALCLLRLFRSSPDIIPGGEWTSRIIHL-----LNDQHMGVVTAATS 220

Human   228 IYLRMIKENSSGYKDLTGSFVTILKQVVGGKLP--VEFNYHSVPAPWLQIQLLRIL--------- 281
            :...::|.|...||......|:.|.::|.....  .::.|:.||||||.::|||:|
  Fly   221 LIDALVKRNPDEYKGCVNLAVSRLSRIVTASYTDLQDYTYYFVPAPWLSVKLLRLLQNYNPVTEE 285

Human   282 -GLLGKDDQRTSELMYDVLDESLRRAELNH-NVTYAILFECVHTVYSIYPKSELLEKAAKCIGKF 344
             |:..:.:: |.|.:.:...|..:..::.| |...|:|||.::.:.....:..||.:|...:|:|
  Fly   286 AGVRARLNE-TLETILNKAQEPPKSKKVQHSNAKNAVLFEAINLIIHSDSEPNLLVRACNQLGQF 349

Human   345 VLSPKINLKYLGLKALTYVIQQD---PTLALQHQMTIIECLDHPDPIIKRETLELLYRITNAQNI 406
            :.:.:.||:||.|:::.::...:   ..:....::.|:......|..:::..::|||.:.:..|.
  Fly   350 LSNRETNLRYLALESMCHLATSEFSHEEVKKHQEVVILSMKMEKDVSVRQMAVDLLYAMCDRGNA 414

Human   407 TVIVQKMLEYL----HQSKEEYVIVNLVGKIAELAEKYAPDNAWFIQTMNAVFSVGGDVMHPDIP 467
            ..|||:||.||    :..:||.|:     |:|.||||||.|..|::..:..:..:.||.:..::.
  Fly   415 EEIVQEMLNYLETADYSIREEMVL-----KVAILAEKYATDYTWYVDVILNLIRIAGDYVSEEVW 474

Human   468 NNFLRLLAEGFDDETEDQQLRLYAVQSYLTLLDMENVFYP---QRFLQVMSWVLGEYSYLLDKET 529
            ...::::            :....||.|......|.:..|   :..::|..::|||:..|:..::
  Fly   475 YRVIQIV------------INREEVQGYAAKTVFEALQAPACHENMVKVGGYILGEFGNLIAGDS 527

Human   530 PEEVIAKLYKLLMN--DSVSSETKAWLIAAVTKLTSQAHSSNTVERLIHEFTISLDTCMRQHAFE 592
            ....:.: :|||.:  ...|..|:|.|::...|..:          |..|...::....|||:
  Fly   528 RSAPLVQ-FKLLHSKYHLCSPMTRALLLSTYIKFIN----------LFPEIRTNIQDVFRQHS-- 579

Human   593 LKHLHENVELMKSLLPVDRSCEDLVVDASLSFLDGFVAEGLSQGAAPYKPPHQRQEEKLSQEKVL 657
                  |:          ||     .||.|                     .||..|.|.
  Fly   580 ------NL----------RS-----ADAEL---------------------QQRASEYLQ----- 597

Human   658 NFEPYGLSFSSSGFTGRQSPAGISLGSDVSGNSAE--TGLKETNSLKLEGIKKLWGKEGYLPK-- 718
                  ||..:|              :||.....|  ....|..|..|..:||  .|.|.:|:
  Fly   598 ------LSIVAS--------------TDVLATVLEEMPSFPERESSILAVLKK--KKPGRVPENE 640

Human   719 -KESKTGDESGALPVPQESIMENVDQAITKKDQSQVLTQSKEEKEKQLLASSLFVGLGSESTINL 782
             :|||:       |.|           :|...|:..|..:...|.....|::..:||.:..:.|:
  Fly   641 IRESKS-------PAP-----------LTSAAQNNALVNNSHSKLNNSNANTDLLGLSTPPSNNI 687

Human   783 LGKADTVSHKFRRKSKVKEAKSGETTSTHNMTCSSFSSLSNVAYEDDYYSNTLHDTGD-KELKKF 846
                                .||.         :|.|:|.:| ..|.|.||:.:::.. ...|||
  Fly   688 --------------------GSGS---------NSNSTLIDV-LGDMYGSNSNNNSSAVYNTKKF 722

Human   847 SLTSE--LLDSESLTELPLVEKFSYCSLSTPSLFANNNMEI----FHPPQSTAASVAKESSLASS 905
            ...:.  |.::|.| ::.:..:|.. :|....||..|..::    |:|....:|..|.:.::...
  Fly   723 LFKNNGVLFENEML-QIGVKSEFRQ-NLGRLGLFYGNKTQVPLTNFNPVLQWSAEDALKLNVQMK 785

Human   906 FLEETTEYIHSNAMEVCNNETISVSSYKIWKDDCLLMVWSVTNKSGLELKSADLEIFPAENFKVT 970
            .:|.|.|                                     :|.:::    ::..||
  Fly   786 VVEPTLE-------------------------------------AGAQIQ----QLLTAE----- 804

Human   971 EQPGCCL------PVMEAESTKSFQYSVQIEKPFTEGNLTGFISYHMMDTHSAQLEFSVN--LSL 1027
                 |:      |.:|.    ||:|                        :..|.:||:.  ||:
  Fly   805 -----CIEDYADAPTIEI----SFRY------------------------NGTQQKFSIKLPLSV 836

Human  1028 LDFIRPLKISSDDFGKLWLSFANDVKQNVKMSESQAALPSALKTLQQKLR---LHIIEIIG-NEG 1088
            ..|..|.:::::.|...|.:.:.:.:::.|:  .:||.|..|...:.||.   :.:::.:. |..
  Fly   837 NKFFEPTEMNAESFFARWKNLSGEQQRSQKV--FKAAQPLDLPGARNKLMGFGMQLLDQVDPNPD 899

Human  1089 LLACQLL-----PSIPCLLHCRVH--ADVLALWFRSS--------CSTLPD 1124
            .:.|..:     ..:.||:....:  |.:..|..|:|        |..|.|
  Fly   900 NMVCAGIIHTQSQQVGCLMRLEPNKQAQMFRLTVRASKETVTREICDLLTD 950

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
AP4E1	NP_031373.2	Adaptin_N	52..596	CDD:396262	140/583 (24%)
		Interaction with TEPSIN. /evidence=ECO:0000269|PubMed:26542808	727..1137		74/432 (17%)
		AP4E_app_platf	1033..1133	CDD:464326	21/111 (19%)
AP-2alpha	NP_995607.1	Adaptin_N	40..603	CDD:396262	152/656 (23%)
		Alpha_adaptinC2	730..839	CDD:197886	29/189 (15%)
		Alpha_adaptin_C	839..947	CDD:426707	20/109 (18%)