Protein Alignment SIRT1 and Sirt1

DIOPT Version :10

• Sequence 1: NP_036370.2
• Gene: SIRT1 / 23411
• HGNCID: 14929
• Length: 747
• Species: Homo sapiens
• Sequence 2: NP_477351.1
• Gene: Sirt1 / 34708
• FlyBaseID: FBgn0024291
• Length: 823
• Species: Drosophila melanogaster
• Alignment Length: 793
• Identity: 306/793 - (38%)
• Similarity: 407/793 - (51%)
• Gaps: 180/793 - (22%)

Human    59 EVPAAARGCP------GA---AAAALWREAEAEAAA-------------AGGEQEAQATAAAGEG 101
            :||...:..|      ||   |:.:...||||||.|             |.||.:.:..||..|.
  Fly    21 QVPDTTQFYPPTKFDFGAEILASTSTEAEAEAEATATTTEPATSELAGKANGEIKTKTLAAREEQ 85

Human   102 DNGPGLQGPSREPPLADNLYDEDDDDEGEEEEEAAAAAIGYRDNLLFGDEIITNGFHSCESDEED 166
            :.|..|:..::.|..:   ..||:|||.||||:....           :|....|.....:::||
  Fly    86 EIGANLEHKTKNPTKS---MGEDEDDEEEEEEDDEEE-----------EEDDEEGITGTSNEDED 136

Human   167 RASHASSS---DWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPE--TIPPPELDDMTLWQIVIN 226
            .:|:.|||   ||..|       ::|:....|..||.::..::|.  |....:.||..||..:.:
  Fly   137 SSSNCSSSVEPDWKLR-------WLQREFYTGRVPRQVIASIMPHFATGLAGDTDDSVLWDYLAH 194

Human   227 ILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLP 291
            :|:||.:|.|...:||.:|.:.|:::.:|||||||||||||||||||||.:|||||||.||||||
  Fly   195 LLNEPKRRNKLASVNTFDDVISLVKKSQKIIVLTGAGVSVSCGIPDFRSTNGIYARLAHDFPDLP 259

Human   292 DPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGI 356
            ||||||||.||::|||||:|||:|||||:||||.||:||.:.:.:|||||||||||||||:||||
  Fly   260 DPQAMFDINYFKRDPRPFYKFAREIYPGEFQPSPCHRFIKMLETKGKLLRNYTQNIDTLERVAGI 324

Human   357 QRIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCP-------------ADEPL---- 404
            ||:|:|||||:||||..|::|.:.:|:|.|||.|.:|.||:|.             .:|.|
  Fly   325 QRVIECHGSFSTASCTKCRFKCNADALRADIFAQRIPVCPQCQPNKEQSVDASVAVTEEELRQLV 389

Human   405 --AIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINRE 467
              .||||:||||||.||:::|..|..|||..||||||||||||||||.||||||..|||||||||
  Fly   390 ENGIMKPDIVFFGEGLPDEYHTVMATDKDVCDLLIVIGSSLKVRPVAHIPSSIPATVPQILINRE 454

Human   468 PLPHLHFDVELLGDCDVIINELCHRLGGE---YAKLCCNPVKLSEITEKPPRTQKELAYLSELPP 529
            .|.||.||||||||.|||||::||||...   :.:|||:...|:|        .|||     :||
  Fly   455 QLHHLKFDVELLGDSDVIINQICHRLSDNDDCWRQLCCDESVLTE--------SKEL-----MPP 506

Human   530 TP---------LHVSEDSSSPERTSPPDSSVIVTLLDQAAKSNDDLD-VSESKG-CME------- 576
            ..         ||....||..||.|..|:..      |:.|||...| :..|.| |.:
  Fly   507 EHSNHHLHHHLLHHRHCSSESERQSQLDTDT------QSIKSNSSADYILGSAGTCSDSGFESST 565

Human   577 ---------------------------------------EKPQ-EVQTSRNV------ESIAEQM 595
                                                   |.|| .|::.|::      :|..||.
  Fly   566 FSCGKRSTAAEAAAIERIKTDILVELNETTALSCDRLGLEGPQTTVESYRHLSIDSSKDSGIEQC 630

Human   596 ENPDLKNVGSSTGEKNERTSVAGTVRKCWPNRVAKEQISRRLD-GNQYLFLPPNRYIFHGAEV-- 657
            :|....:....:....|..:||.::......|..::..:.||. |..|.......|:|.||:|
  Fly   631 DNEATPSYVRPSNLVQETKTVAPSLTPIPQQRGKRQTAAERLQPGTFYSHTNNYSYVFPGAQVFW 695

Human   658 ---YSDSEDDVLSSS---------SCGSN---SDSGTCQ------SPSLEEPMEDESEIEEFYNG 701
               |||.:|:....|         :.|.|   .|...|.      ||.|...:|... :.:..||
  Fly   696 DNDYSDDDDEEEERSHNRHSDLFGNVGHNYKDDDEDACDLNAVPLSPLLPPSLEAHI-VTDIVNG 759

Human   702 LEDEPDVPERAGG 714
             .:|| :|..:.|
  Fly   760 -SNEP-LPNSSPG 770

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
SIRT1	NP_036370.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..135		31/97 (32%)
		Interaction with H1-4. /evidence=ECO:0000269|PubMed:15469825	2..268		73/235 (31%)
		Interaction with CLOCK. /evidence=ECO:0000250|UniProtKB:Q923E4	2..139		31/101 (31%)
		Nuclear localization signal. /evidence=ECO:0000250	32..39
		Nuclear export signal. /evidence=ECO:0000250	138..145		0/6 (0%)
		Interaction with CCAR2	143..541		222/433 (51%)
		Nuclear localization signal. /evidence=ECO:0000250	223..230		1/6 (17%)
		SIRT1	254..489	CDD:238699	174/253 (69%)
		Required for interaction with the sumoylated form of CCAR2. /evidence=ECO:0000269|PubMed:25406032	256..259		2/2 (100%)
		Nuclear export signal. /evidence=ECO:0000250	425..431		2/5 (40%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	523..549		9/34 (26%)
		Phosphorylated at one of three serine residues	538..540		1/1 (100%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	562..587		10/73 (14%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	663..726		16/70 (23%)
Sirt1	NP_477351.1	SIRT1	222..476	CDD:238699	174/253 (69%)