Protein Alignment FOXO1 and FKH2

DIOPT Version :10

• Sequence 1: NP_002006.2
• Gene: FOXO1 / 2308
• HGNCID: 3819
• Length: 655
• Species: Homo sapiens
• Sequence 2: NP_014331.3
• Gene: FKH2 / 855656
• SGDID: S000005012
• Length: 862
• Species: Saccharomyces cerevisiae
• Alignment Length: 767
• Identity: 171/767 - (22%)
• Similarity: 265/767 - (34%)
• Gaps: 236/767 - (30%)

Human     6 QVVEIDPDFEPLPRP----------------RSCTWPLPRPEFSQSNSA-----TSSP------- 42
            |::.|.||.:|:..|                .....||.|....|||.|     ||:.
Yeast   175 QMMFILPDSDPVVAPICIEHLMPNLINMFGLEGNNNPLLRDIIKQSNYAKQRQLTSNQQIKGFKL 239

Human    43 ------APSGSAAANPDAAAGLPSASAAAVSADFMSNLSLLEESEDFPQAPGSVAAAVAAAAAAA 101
                  ||.||.|....:..|:           |.:|.:...::..|.....:..|:...:...
Yeast   240 YGSGGNAPFGSGANLGPSEQGI-----------FNNNNNSKNKNGYFTSINPNYTASTTTSNTI- 292

Human   102 ATGGLCGDFQGPEAGCLHPAPPQPPPPGPLSQH--------PPVPPAAAGPLAGQPRKSSSSRRN 158
                      .|:|     |.||.||...::.:        ...|.|    |......|....||
Yeast   293 ----------NPQA-----ASPQGPPNTIIAANFVDSYKSSNAYPQA----LDFTSDLSHDENRN 338

Human   159 AWGNLSYADLITKAIESSAEKRLTLSQIYEWMVKSVPYFKDKGDSNSSAGWKNSIRHNLSLHSKF 223
            .....|||.:||:||.||.|..::|:.||:::..:..|::     .:.:||:|||||||||:..|
Yeast   339 VKPPHSYATMITQAILSSPEGVISLADIYKYISSNYAYYR-----FAKSGWQNSIRHNLSLNKAF 398

Human   224 IRV---QNEGTGKSSWWMLNP----------EGGKSGKSPRRRAASMDNNSKFAK---------- 265
            .:|   .|| .||...|.::.          ..||.||..|..:.:.......||
Yeast   399 EKVPRRPNE-PGKGMKWRISESYQQEFLNKWNTGKVGKIRRGSSVARQLQLHMAKFNSLPMEMDY 462

Human   266 --SRSRAAKKKASLQSGQ----------EG-------AGDSPGSQFSKWPASPGSHSND------ 305
              |.:.|...|..|||..          ||       .|:.|.||.|:   .|.||.|.
Yeast   463 RLSLNMAQPPKRQLQSHNVLEPSNNNIIEGFVQHVPSKGNLPASQQSQ---PPVSHQNQSQQPPP 524

Human   306 -------DFDNWSTFRPRTSSNASTISGRLSPIMTEQDDLGEGDVHSMVYPPSAAKMASTLPSLS 363
                   .|    ||....:.|.:.    ..||.|.|          :..|.|.|.:
Yeast   525 QEQRQEIQF----TFADTQNRNIAL----ARPIKTPQ----------LQAPNSNANL-------- 563

Human   364 EISNPENMENLLDNLNLLSSPTSLTVSTQSSPGTMMQQTP-----CYSFAPPNTS-LNSPSPNYQ 422
               |..||:...::|:    |.::::|      .|.:|:|     .::.|..|:. :|:.|.:..
Yeast   564 ---NQNNMKEYKESLH----PPAISIS------QMNRQSPNNALVSFTNACANSKIINNISDSAD 615

Human   423 KYT--YGQSSMSPLPQMPIQTLQDNKSSYGGMSQYNCAPGLLKELLTSDSPPHNDI-MTPVDPGV 484
            |.|  .|.:.|: ||.:...:|.:|              |.|:...|:.|...|.: .|......
Yeast   616 KSTNNNGGTKMN-LPAISTSSLDEN--------------GNLEPTTTTSSGNSNSVPQTGTTTSS 665

Human   485 AQPNSRVLGQ---NVMMGPN-----SVMSTY-GSQASHNKMMNP----SSHTHPGHAQ----QTS 532
            ...||..|.|   .::..|.     :.|..| ..:.|.|:..:|    |::|:...|.    |||
Yeast   666 LAANSLRLSQPYDTLLRSPTKAFHITAMEAYTPERGSANRARSPLHSNSNNTNNNGANNSNLQTS 730

Human   533 AVNGRPL-----PHTVSTMPHTSGMNRLTQVKTPVQVPLPHP-----MQMSALGGYSSVSSCNGY 587
            .:..:..     .:.:.:|...:...|||...:..|.....|     :|.|:.....:..|  |.
Yeast   731 GMENKQTGLVLDSNVLKSMESNNDNRRLTPSTSKSQNVKSSPGVWNLLQFSSTNNTPAADS--GG 793

Human   588 GRMGLLHQEKLPSDLDGMFIERLDCDMESIIRNDLMDGDTLDFNFDNVLPNQ 639
            .:.|......:.:..:.......|.|..|   |||   :|.|.| .:.|.||
Yeast   794 NKRGFSINPDIKAKENENATSEKDSDSNS---NDL---ETKDIN-SSPLKNQ 838

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
FOXO1	NP_002006.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..63		21/90 (23%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	116..158		9/49 (18%)
		FH_FOXO1	151..249	CDD:410834	38/110 (35%)
		DNA-binding. /evidence=ECO:0000269|PubMed:18786403	211..218		6/6 (100%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	234..344		33/161 (20%)
		DNA-binding. /evidence=ECO:0000269|PubMed:18786403	234..237		0/2 (0%)
		Nuclear localization signal	251..253		1/1 (100%)
		Sufficient for interaction with NLK. /evidence=ECO:0000250|UniProtKB:Q9R1E0	283..563		68/330 (21%)
		Required for interaction with RUNX2. /evidence=ECO:0000250|UniProtKB:Q9R1E0	363..459		21/103 (20%)
		FOXO_KIX_bdg	423..504	CDD:465228	19/91 (21%)
		Required for interaction with SIRT1. /evidence=ECO:0000250|UniProtKB:Q9R1E0	462..466		1/3 (33%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	507..537		10/38 (26%)
		FOXO-TAD	597..633	CDD:435506	9/35 (26%)
FKH2	NP_014331.3	COG5025	1..610	CDD:227358	117/513 (23%)