Protein Alignment FOXO1 and HCM1

DIOPT Version :10

• Sequence 1: NP_002006.2
• Gene: FOXO1 / 2308
• HGNCID: 3819
• Length: 655
• Species: Homo sapiens
• Sequence 2: NP_009991.2
• Gene: HCM1 / 850429
• SGDID: S000000661
• Length: 564
• Species: Saccharomyces cerevisiae
• Alignment Length: 589
• Identity: 146/589 - (24%)
• Similarity: 211/589 - (35%)
• Gaps: 203/589 - (34%)

Human   127 PPGPLS--QHPPVPPAAA------------GPLAGQPRKSSSSRRNAWGNL------SYADLITK 171
            |..|||  ...|:.|:.|            |.|..:....|..||...|.|      |||.||..
Yeast    56 PSHPLSPDHSSPIAPSKAKRQRSDTCARSNGNLTLEEILQSLERRRINGELAKKPPYSYATLICL 120

Human   172 AIESSAEKRLTLSQIYEWMVKSVPYFKDKGDSNSSAGWKNSIRHNLSLHSKFIRVQNEGTGKSSW 236
            ||..|.|.:|||||||.|:....||:|.|     .|.|:|||||||||:..||:.:....||..:
Yeast   121 AILQSQEGKLTLSQIYHWIHVHFPYYKQK-----DASWQNSIRHNLSLNDAFIKTEKSCDGKGHF 180

Human   237 WMLNPEGGKSGKSPRRRAASMDNNSKFAKSRSRAAK-KKASLQS-GQEGAGDSPGSQFSKWPASP 299
            |.:.|..                .:||.|..:|..: .|.|||. |:....||...:..:..:..
Yeast   181 WEVRPGA----------------ETKFFKGENRGYEFVKDSLQDIGKYFEIDSTLDELEQVESGE 229

Human   300 GSHSNDDFDNWSTFRPRTSSNASTISGRLSPIMTEQDDLGEGDVHSMVYPPSAAKMAST------ 358
            |   |||.                      |...|:::.|:       :|....::.|:
Yeast   230 G---NDDL----------------------PDEEEREEAGK-------FPSIEIQLNSSPILRVS 262

Human   359 ----LPSL---SEISNP-ENMENL-------LDNLNLLSSP-------TSLTVSTQSSPGTMMQQ 401
                :|.|   :.:.|| ||:|::       ::|::.|..|       |||.:.:..:.....|.
Yeast   263 QLHHIPQLKTDNSVLNPHENLESMRNMIENDVNNIDSLEPPYVMKKYHTSLGLPSLVNAKDHFQA 327

Human   402 -------TPCYSF-APPNTSLNSPSPNYQKYTYGQSS----MSPLPQMPIQTLQDNKSSYGGMSQ 454
                   |....| ..|.||..||. |::||....:|    :|||           :|:.|
Yeast   328 GVKNNNITQANRFNTLPITSAKSPQ-NFRKYFTSFNSNFEDLSPL-----------RSNVG---- 376

Human   455 YNCAPGLLKELLTSDSPPHNDIMTPVDPGVAQPNSRVLGQNVMMGPNSVMSTYGSQASHNKMMNP 519
               |..||      |..|::.:.......:|          :|..|.|..|...||..    ..|
Yeast   377 ---AGSLL------DPLPYSPLKLYDQKNLA----------LMSKPQSQQSYSNSQLP----PPP 418

Human   520 SSHTHPGHAQQTSAVNGRPLPHTVSTMPHTSGMNRLTQVKTPVQVPLPHPMQMSALGGYSSVSSC 584
            |||             |..|..| ..|.|:.|:.     |||.::             .|:....
Yeast   419 SSH-------------GSDLLKT-PKMRHSDGLE-----KTPSRL-------------ISTPKDG 451

Human   585 NGYGRMGLLHQEKLPSDLDGMFIERLDCD-----MESIIRNDLMDGDTLDFNFDNVLPNQSFPHS 644
            |     .:|.:.:.||.|    .|.|.|.     :|:.||.....|.||:   ..:.|.:|....
Yeast   452 N-----SILRKWQTPSHL----FEDLYCSPLFRAIETPIRYITTPGGTLE---TQISPRKSSAPD 504

Human   645 VKTT 648
            |.|:
Yeast   505 VLTS 508

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
FOXO1	NP_002006.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..63
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	116..158		11/44 (25%)
		FH_FOXO1	151..249	CDD:410834	43/103 (42%)
		DNA-binding. /evidence=ECO:0000269|PubMed:18786403	211..218		6/6 (100%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	234..344		20/111 (18%)
		DNA-binding. /evidence=ECO:0000269|PubMed:18786403	234..237		0/2 (0%)
		Nuclear localization signal	251..253		0/1 (0%)
		Sufficient for interaction with NLK. /evidence=ECO:0000250|UniProtKB:Q9R1E0	283..563		66/319 (21%)
		Required for interaction with RUNX2. /evidence=ECO:0000250|UniProtKB:Q9R1E0	363..459		28/122 (23%)
		FOXO_KIX_bdg	423..504	CDD:465228	17/84 (20%)
		Required for interaction with SIRT1. /evidence=ECO:0000250|UniProtKB:Q9R1E0	462..466		1/3 (33%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	507..537		6/29 (21%)
		FOXO-TAD	597..633	CDD:435506	12/40 (30%)
HCM1	NP_009991.2	COG5025	20..564	CDD:227358	146/589 (25%)