Protein Alignment FOXO1 and foxo1

DIOPT Version :10

• Sequence 1: NP_002006.2
• Gene: FOXO1 / 2308
• HGNCID: 3819
• Length: 655
• Species: Homo sapiens
• Sequence 2: NP_001008016.1
• Gene: foxo1 / 493378
• XenbaseID: XB-GENE-482528
• Length: 626
• Species: Xenopus tropicalis
• Alignment Length: 682
• Identity: 451/682 - (66%)
• Similarity: 507/682 - (74%)
• Gaps: 83/682 - (12%)

Human     1 MAEA----PQVVEIDPDFEPLPRPRSCTWPLPRPEFSQSNSATSSPAPSGSAAANPDAAAGLPSA 61
            ||||    |..||:||||||..||||||||||||||:.|:||.||||||    ..|:.|||
 Frog     1 MAEAPLPPPPGVEVDPDFEPFSRPRSCTWPLPRPEFNPSSSANSSPAPS----LQPEPAAG---- 57

Human    62 SAAAVSADFMSNLSLLEESEDFPQAPGSVAAAVAAAAAAAATGGLCGDFQGPEAGCLHPAPPQPP 126
                 :.||:||||||||||||..|...               |:||||.     |......|||
 Frog    58 -----NVDFLSNLSLLEESEDFDPAEAL---------------GVCGDFP-----CQDIRQLQPP 97

Human   127 PPGPLSQHP---------PVPPAAAGPL-------AGQPRKSSSSRRNAWGNLSYADLITKAIES 175
            .|   .|||         |..|:|..|.       |.||||||||||||||||||||||::||||
 Frog    98 VP---QQHPQQQAGTLCAPSVPSALSPASSPSPLGAQQPRKSSSSRRNAWGNLSYADLISQAIES 159

Human   176 SAEKRLTLSQIYEWMVKSVPYFKDKGDSNSSAGWKNSIRHNLSLHSKFIRVQNEGTGKSSWWMLN 240
            |.||||||||||:|||||||||||||||||||||||||||||||||||:|||||||||||||:||
 Frog   160 SPEKRLTLSQIYDWMVKSVPYFKDKGDSNSSAGWKNSIRHNLSLHSKFVRVQNEGTGKSSWWILN 224

Human   241 PEGGKSGKSPRRRAASMDNNSKFAKSRSRAAKKKASLQSGQEGAGDSPGSQFSKWPASPGSHSND 305
            |||||:|||||||||||||||||||||.|||||||::||.|:|:.|||||||||||.||.|.|||
 Frog   225 PEGGKNGKSPRRRAASMDNNSKFAKSRGRAAKKKATMQSSQDGSSDSPGSQFSKWPGSPSSQSND 289

Human   306 DFDNWSTFRPRTSSNASTISGRLSPIMTEQDDLGEGDVHSMVYPPSAAKMASTLPSLSEISNPEN 370
            ||:.|||||||||||||||||||||||.||||||:.|||::||||||.|:.||||||||:.|.||
 Frog   290 DFEAWSTFRPRTSSNASTISGRLSPIMPEQDDLGDADVHNLVYPPSATKLTSTLPSLSEMGNSEN 354

Human   371 MENLLDNLNLLSSPTSLTVSTQSSPGTMMQQTPCYSFAPPNTSLNSPSPNYQKYTYGQSSMSPLP 435
            ||||||||||||..|    ||||||.:||||:. |.|..|||||.||:..|:||:|.|:.|:|:.
 Frog   355 MENLLDNLNLLSPNT----STQSSPASMMQQSG-YLFTSPNTSLGSPNSEYRKYSYAQTGMNPVS 414

Human   436 QMPIQTLQDNKSSYGGMSQYNCAPGLLKELLTSDSPPHNDIMTPVDPGVAQPNSRVLGQNVMMGP 500
            |||:||:.:|||.|..:.||....|||||||||||||||||:||||..|:|.|||||.||.:|.|
 Frog   415 QMPMQTVPENKSGYRAVGQYPVPAGLLKELLTSDSPPHNDILTPVDSAVSQANSRVLAQNSLMAP 479

Human   501 NSVMSTYGSQASHNKMMNPSSHTH-----PGHAQQTSAVNGRPLPHTVSTMPHTSGMNRLTQVKT 560
            :|||.|||||.:||||   |||.|     |.|    .:||||       ||.|.||:|||:.|||
 Frog   480 SSVMPTYGSQPTHNKM---SSHPHSHQPPPNH----PSVNGR-------TMTHNSGINRLSTVKT 530

Human   561 PVQVPLPHPMQMSALGGYSSVSSCNGYGRMGL--LHQEKLPSDLDGMFIERLDCDMESIIRNDLM 623
            .||||:|.|:||:::|.| .|:|||||||:|:  :|||.||||||.||||.||||||||||||||
 Frog   531 SVQVPMPQPIQMTSMGSY-PVNSCNGYGRVGIVSIHQEILPSDLDDMFIESLDCDMESIIRNDLM 594

Human   624 DGDTLDFNFDNVLPNQSFPHSVKTTTHSWVSG 655
            :....|||||::||||||||||.|||||||||
 Frog   595 EDGEADFNFDSILPNQSFPHSVTTTTHSWVSG 626

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
FOXO1	NP_002006.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..63		40/65 (62%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	116..158		22/57 (39%)
		FH_FOXO1	151..249	CDD:410834	90/97 (93%)
		DNA-binding. /evidence=ECO:0000269|PubMed:18786403	211..218		6/6 (100%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	234..344		92/109 (84%)
		DNA-binding. /evidence=ECO:0000269|PubMed:18786403	234..237		2/2 (100%)
		Nuclear localization signal	251..253		1/1 (100%)
		Sufficient for interaction with NLK. /evidence=ECO:0000250|UniProtKB:Q9R1E0	283..563		185/284 (65%)
		Required for interaction with RUNX2. /evidence=ECO:0000250|UniProtKB:Q9R1E0	363..459		55/95 (58%)
		FOXO_KIX_bdg	423..504	CDD:465228	50/80 (63%)
		Required for interaction with SIRT1. /evidence=ECO:0000250|UniProtKB:Q9R1E0	462..466		3/3 (100%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	507..537		16/34 (47%)
		FOXO-TAD	597..633	CDD:435506	27/35 (77%)
foxo1	NP_001008016.1	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..57		38/59 (64%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	90..142		21/54 (39%)
		FH_FOXO1	135..233	CDD:410834	90/97 (93%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	218..319		85/100 (85%)
		FOXO_KIX_bdg	402..483	CDD:465228	50/80 (63%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	484..519		21/48 (44%)
		FOXO-TAD	569..606	CDD:435506	29/36 (81%)