Protein Alignment FOXO1 and FoxK

DIOPT Version :10

• Sequence 1: NP_002006.2
• Gene: FOXO1 / 2308
• HGNCID: 3819
• Length: 655
• Species: Homo sapiens
• Sequence 2: NP_001261701.1
• Gene: FoxK / 39252
• FlyBaseID: FBgn0036134
• Length: 760
• Species: Drosophila melanogaster
• Alignment Length: 619
• Identity: 138/619 - (22%)
• Similarity: 195/619 - (31%)
• Gaps: 249/619 - (40%)

Human    15 EPLPRPRSCTWPLP----RPEFSQSNSATSSPAPSG-SAAANPDAAAGLPSASAAAVSADF---- 70
            :||..|:.|.:..|    |.||.....||||.|..| |.:.......|:....|..:....
  Fly   230 DPLRLPQRCYFRFPSTEIRIEFESYVPATSSDAIDGHSPSLVVGGGGGVAGGGAHVIITPLPRDE 294

Human    71 -------------------------MSNLSLLEESED-------FPQAP---------------- 87
                                     ..:|.|.::.:.       .|..|
  Fly   295 LHQQQLHHHQQQPHLQQQQHPHHPPAHHLPLQQQQQQQQPAHHPLPHTPHHPLHHTALHQQQQRS 359

Human    88 GSVAAAVAAAAAAAATGGLCGDFQGPEAGCLHP----------APPQPPPPGPLSQHPPVPPAAA 142
            ||:..|..|.|||....|     .||  |...|          ..|...|.|.:|        ||
  Fly   360 GSIVVAPPAGAAAHLIAG-----DGP--GIYSPLKISIPKKEQKSPYLSPTGTIS--------AA 409

Human   143 GPLAGQPR----KSSSSRRNAWGN----------------------LSYADLITKAIESSAEKRL 181
            ......||    ::..:..|.:||                      .|||.||.:||.::.:|:|
  Fly   410 NSCPASPRQGFIQNQPNNYNNYGNNNTQDLFQTPSTASYNHNEKPPYSYAQLIVQAISAAPDKQL 474

Human   182 TLSQIYEWMVKSVPYFKDKGDSNSSAGWKNSIRHNLSLHSKFIRV---QNEGTGKSSWWMLNPEG 243
            |||.||.::||..||::    ..::.||:|||||||||:..||:|   |:| .||.|:|.::|:.
  Fly   475 TLSGIYSFIVKHYPYYR----KETNKGWQNSIRHNLSLNRYFIKVARSQDE-PGKGSFWRIDPDS 534

Human   244 GKSGKSPRRRAASMDNNSKFAKSRSRAAKKKASLQSGQEGAGDSPGSQFSKWPASPGSHSNDDFD 308
            |         |..:|::.|..:.||                             |.|
  Fly   535 G---------AKLIDHSYKKRRQRS-----------------------------SQG-------- 553

Human   309 NWSTFRPRTSSNASTISGRLSPIMTEQDDLGEGDVHSMVYPPSAAKMASTLPSLSEISNPENMEN 373
                |||...                                        :|..:.:| |.:|:|
  Fly   554 ----FRPPYG----------------------------------------MPRSAPVS-PSHMDN 573

Human   374 LLDNLNLLSSPTSLTV--STQSSPGTMMQQTPCYSFAPPNTSLNSPSPNYQKYTYGQSSMSPLPQ 436
            ..:     |||....|  |...|||..::|..    |.|....||.:.:.|:....|       |
  Fly   574 SRE-----SSPLQDIVLQSAPGSPGMSLEQRA----ADPEIIYNSQNAHQQQQQQQQ-------Q 622

Human   437 MPIQTLQDNKSSYGGMSQYNCAPGLLKELLTSDSPPHNDIMTPVDPGVAQPNSRVLGQNVMMGPN 501
            ...|||.:|.:.|...|.|                    .:|....|||.|.:.|.|.....|..
  Fly   623 QQQQTLSNNSNQYSSGSPY--------------------YVTNQSSGVATPQTHVEGSAASGGGG 667

Human   502 SVMSTYGS--QASHNKMMNPSSHTHPGHAQQTSA 533
            .  ...|:  ....|.:|...:..|..|.||..|
  Fly   668 G--GGVGALLALKRNHVMGGGASQHTLHQQQAVA 699

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
FOXO1	NP_002006.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..63		16/52 (31%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	116..158		10/55 (18%)
		FH_FOXO1	151..249	CDD:410834	43/122 (35%)
		DNA-binding. /evidence=ECO:0000269|PubMed:18786403	211..218		6/6 (100%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	234..344		14/109 (13%)
		DNA-binding. /evidence=ECO:0000269|PubMed:18786403	234..237		1/2 (50%)
		Nuclear localization signal	251..253		0/1 (0%)
		Sufficient for interaction with NLK. /evidence=ECO:0000250|UniProtKB:Q9R1E0	283..563		49/255 (19%)
		Required for interaction with RUNX2. /evidence=ECO:0000250|UniProtKB:Q9R1E0	363..459		27/97 (28%)
		FOXO_KIX_bdg	423..504	CDD:465228	17/80 (21%)
		Required for interaction with SIRT1. /evidence=ECO:0000250|UniProtKB:Q9R1E0	462..466		0/3 (0%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	507..537		8/29 (28%)
		FOXO-TAD	597..633	CDD:435506
FoxK	NP_001261701.1	FHA_FOXK	151..252	CDD:438740	7/21 (33%)
		FH_FOXK	453..531	CDD:410800	38/82 (46%)