Protein Alignment FOXO1 and sep1

DIOPT Version :10

• Sequence 1: NP_002006.2
• Gene: FOXO1 / 2308
• HGNCID: 3819
• Length: 655
• Species: Homo sapiens
• Sequence 2: NP_596301.1
• Gene: sep1 / 2540866
• PomBaseID: SPBC4C3.12
• Length: 663
• Species: Schizosaccharomyces pombe
• Alignment Length: 642
• Identity: 133/642 - (20%)
• Similarity: 226/642 - (35%)
• Gaps: 195/642 - (30%)

Human    81 EDFPQAPGSVAAAVAAAAAAAATGGLCGDFQGPEAGCLHP-----------APPQP-PPPGPLSQ 133
            :..|..| .||:.:....|......: ..||.|.:....|           .||.| |||...|.
pombe    36 QSLPPYP-KVASKLKGVVAGGKENNI-ASFQKPSSKATRPYIPSYTRLTYSVPPLPIPPPSEQSL 98

Human   134 HPPV--PPAAAGPLAGQPRK----SSSSRRNAWGNLSYADLITKAIESSAEKRLTLSQIYEWMVK 192
            ...:  .|:.:...:.:|.:    ....::..:   |||.||..:|..|.::|||||.||:|:..
pombe    99 DTIIYRNPSVSSSQSQEPEEFFLPLDDGKKPPY---SYAMLIGMSIIRSPDRRLTLSAIYDWISN 160

Human   193 SVPYFKDKGDSNSSAGWKNSIRHNLSLHSKFIRVQNEGT--GKSSWWMLNPEGGKSGKSPRRRAA 255
            :..::     :.|:.||:|||||||||:..|::::....  ||..:|.:.|
pombe   161 TFSFY-----NKSNNGWQNSIRHNLSLNKAFMKIERPRNLPGKGHFWSIRP-------------- 206

Human   256 SMDNNSKFAKSR-------SRAAKKKASLQSGQEGAGDSPGSQFSKWPASPG--SHSNDDFDNWS 311
              .:..:|.|.:       ||.|.....:.|..:....:..|.|:.:..||.  :..:....|:.
pombe   207 --GHEEQFLKLKLRKPGVNSRPAPPVQDVTSSTKYGSSTGSSGFNTFNTSPHIFNQRHQYLQNYY 269

Human   312 TFRPRTSSNASTISG----RLSPIMTEQDDLGEGDVHSMVYPPSAAKMASTLPSLSEISNPENME 372
            |   .:.:|..|||.    ...|:.::|         ..|..|.             |..|.::|
pombe   270 T---ASLTNIPTISNVNATNFHPLHSQQ---------PYVDTPG-------------IDAPSDLE 309

Human   373 NLLDNLNLLSSPTSLTVSTQSSPGTMMQQTPCYSFAPPNTSLNSPSPNYQKYTYGQSSMSPLPQM 437
            ....:|. :||..|:|...||...:            |:..|:||:          ||.||...:
pombe   310 AKFSDLG-VSSVVSVTSPLQSCTNS------------PSPPLSSPA----------SSASPSESL 351

Human   438 PIQTL--QDNKSSYGGMSQYNCAPGLLKELLTSDSPPHNDIMTPVD-PGVAQPNSRVLGQNVMMG 499
            ..::|  :..||.           ||.|:....:.||.:.:...|: |.|         .:.::|
pombe   352 RNESLGIKSAKSL-----------GLNKDDAPVEGPPVSHLEKDVETPSV---------HDSVLG 396

Human   500 PNSVMSTYGSQASHNKMMNPSSHTHPGHAQQTSAVNGRPLPHTVSTMPHTSGMNRLTQVKTPVQV 564
            .|..::..|.:...:...|         ..|..||....||.: .|:.:.||:  |.:....:..
pombe   397 FNDTVTNLGKKGLKDGTTN---------TLQIPAVRLPSLPSS-PTIKNPSGL--LLKRSNSIDF 449

Human   565 PLPHPMQMSALGGYSSVSSCNGYGRMGLLHQEKLPSDLDGM--------------FIERL----- 610
            |.|.......|..:......:.|.:..||  ..:.||:.|:              .::.|
pombe   450 PTPPKALCPKLFCFRDDIVADDYTKFSLL--SPIRSDMSGISASPNTNLKEHRTRILQMLATPDA 512

Human   611 -------DCDME---------SIIRN----------------DLMDGDTLDFNFDNV 635
                   ..|.|         ||:||                .|:||.:|.:..:|:
pombe   513 KQLSSLTSSDAEFWSVTPLKSSILRNGDASKQVTLSESPKGDSLLDGGSLSYFTNNI 569

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
FOXO1	NP_002006.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..63
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	116..158		10/59 (17%)
		FH_FOXO1	151..249	CDD:410834	32/103 (31%)
		DNA-binding. /evidence=ECO:0000269|PubMed:18786403	211..218		6/6 (100%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	234..344		20/122 (16%)
		DNA-binding. /evidence=ECO:0000269|PubMed:18786403	234..237		0/2 (0%)
		Nuclear localization signal	251..253		0/1 (0%)
		Sufficient for interaction with NLK. /evidence=ECO:0000250|UniProtKB:Q9R1E0	283..563		56/288 (19%)
		Required for interaction with RUNX2. /evidence=ECO:0000250|UniProtKB:Q9R1E0	363..459		21/97 (22%)
		FOXO_KIX_bdg	423..504	CDD:465228	17/83 (20%)
		Required for interaction with SIRT1. /evidence=ECO:0000250|UniProtKB:Q9R1E0	462..466		1/3 (33%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	507..537		5/29 (17%)
		FOXO-TAD	597..633	CDD:435506	14/86 (16%)
sep1	NP_596301.1	COG5025	20..663	CDD:227358	133/642 (21%)