Protein Alignment KIF21B and Klp54D

DIOPT Version :10

• Sequence 1: NP_001239029.1
• Gene: KIF21B / 23046
• HGNCID: 29442
• Length: 1637
• Species: Homo sapiens
• Sequence 2: NP_001303355.1
• Gene: Klp54D / 47216
• FlyBaseID: FBgn0263076
• Length: 760
• Species: Drosophila melanogaster
• Alignment Length: 668
• Identity: 201/668 - (30%)
• Similarity: 295/668 - (44%)
• Gaps: 145/668 - (21%)

Human     3 GQGDCC-----VKVAVRIRPQLSKEKIEGCHICTSVTPGEPQVLL-GKD------------KAFT 49
            |..:|.     :.|.||:||...|||.:. |..|...||..||:| |.|            :.||
  Fly   156 GSEECSTPEDNINVVVRVRPLNDKEKRDR-HGSTLQFPGNGQVILEGNDVGQKRSHNRDSVRVFT 219

Human    50 YDFVFDLDTWQEQI--YSTCVSKLIEGCFEGYNATVLAYGQTGAGKTYTMGTGFDMAT-----SE 107
            |:.||:....||.|  ||. :.::||...||::.|...|||||:|||:|:....|:..     .:
  Fly   220 YNVVFEPGATQEDILDYSG-IKRIIEMGIEGFSCTAFCYGQTGSGKTHTLTGPPDLFVGKPNPKD 283

Human   108 EEQGIIPRAIAHLFGGIAERKRRAQEQGVAGPEFKVSAQFLELYNEEILDLFD--STRDPDTRHR 170
            ...|:|.|:..:||..|..||         ...:.:.|.|:|:|||.::||.:  |.|.|
  Fly   284 PRHGLIFRSFLYLFQLIKNRK---------DVNYVLKASFMEIYNERVIDLLNPGSARKP----- 334

Human   171 RSNIKIH-EDANGGIYTTGVTSRLIHSQEELIQCLKQGALSRTTASTQMNVQSSRSHAIFTIHLC 234
               :.:. ...:||.:...:.:......::|:..|::|..:|...|..||..|||||.|.|:|:
  Fly   335 ---LAVRWSKKSGGFFVENLFTVDCEELDDLLAVLEEGMRNRAVGSHAMNDHSSRSHTILTVHI- 395

Human   235 QMRMCTQPDLVNEAVTGLPDGTPPSSEYETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCG 299
                             |.|.......:.:...|.:||||||||..|:|.:.|:..:|..:||..
  Fly   396 -----------------LSDQQTDGGVFLSKHGKINFVDLAGSELTKKTMSEGKTLEEANNINKS 443

Human   300 LLALGNVISALGDQSKKVVHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYA 364
            |:.||..||:|.|..|:..|:|||||:||:||.|||.||..|:|||||||:..:..||||||:||
  Fly   444 LMVLGYCISSLSDSKKRTGHIPYRDSQLTKLLADSLAGNGVTLMIACVSPAHYNHAETLNTLRYA 508

Human   365 NRARNIKNKVVVNQDKTSQQISALRAEIARLQMELMEYKAGKRVIGEDGAEGYSDLFRENAMLQK 429
            :||:.|:.|.|:..|.....|.:|:.:|..||||....||
  Fly   509 SRAKRIRTKPVIKMDPREALILSLKRDIHALQMENDHLKA------------------------- 548

Human   430 ENGALRLRVKAMQEAIDAINNRVTQL--MSQEANLLLAKAG-------DGNEAIGALIQNYIREI 485
               ||.|..:|........|....||  :|....:.:.|..       ||:| :..|::.|:.|.
  Fly   549 ---ALNLHHQAAPNGGPVENLLELQLDRVSSGGGVPVPKVDLQRLPELDGSE-LAELVKLYMVEN 609

Human   486 EELR-----------TKLLESEAM---NESLRRSL---SRASARSPYSLGASPAAPAFGGSPASS 533
            |.||           |.|.:.|.:   ||.|.:.|   ::...|||    ..||.||.  ||.:.
  Fly   610 ESLRQENNHLFTVRETILRDQEIVCRENERLLKKLEDVNKVCVRSP----LIPARPAI--SPTTG 668

Human   534 MEDASEVIRRAKQDL----------ERLKKKEVRQRRKSPEKEAFKKR-AK--------LQQENS 579
            .|.....|....:.|          |:...:...:|..:.:|...:|| ||        .::.:|
  Fly   669 KETPGTEIWTNPEPLQSPPGPDLPIEQRMSENANRRTDTAQKRIDQKRIAKNILIMANAFRKPDS 733

Human   580 EETDENEAEEEEEERDES 597
            :.|.|.....||....:|
  Fly   734 DITKELNLNPEEAHAKQS 751

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
KIF21B	NP_001239029.1	KISc_KIF4	7..371	CDD:276823	137/391 (35%)
		CwlO1	379..>539	CDD:443091	45/185 (24%)
		Interaction with TRIM3. /evidence=ECO:0000250|UniProtKB:Q9QXL1	400..1099		52/243 (21%)
		Smc	<472..>1064	CDD:440809	39/162 (24%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	509..538		10/28 (36%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	552..628		12/55 (22%)
		EnvC	633..>842	CDD:443969
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	830..865
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	880..906
		Rcc_KIF21B	929..1010	CDD:410203
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1194..1251
		WD40	1305..1613	CDD:238121
		WD 1	1306..1343
		WD40 repeat	1312..1346	CDD:293791
		WD 2	1346..1384
		WD40 repeat	1351..1387	CDD:293791
		WD40 repeat	1407..1450	CDD:293791
		WD 3	1410..1448
		WD 4	1451..1493
		WD40 repeat	1456..1500	CDD:293791
		WD 5	1502..1539
		WD40 repeat	1509..1541	CDD:293791
		WD 6	1543..1582
		WD40 repeat	1548..1572	CDD:293791
		WD 7	1585..1622
Klp54D	NP_001303355.1	KISc	166..520	CDD:214526	138/390 (35%)