Protein Alignment Usp9x and Usp32

DIOPT Version :10

• Sequence 1: XP_006527649.1
• Gene: Usp9x / 22284
• MGIID: 894681
• Length: 2575
• Species: Mus musculus
• Sequence 2: NP_001262074.2
• Gene: Usp32 / 40169
• FlyBaseID: FBgn0036913
• Length: 1779
• Species: Drosophila melanogaster
• Alignment Length: 2124
• Identity: 378/2124 - (17%)
• Similarity: 627/2124 - (29%)
• Gaps: 866/2124 - (40%)

Mouse   290 TDEELKKEAKNEAKNDALSMIIKSLKNLASRVPGQEETVKNLEIFRLKMILRLLQISSFNGKMNA 354
            |..|..|...|...|||.:.||||            :.|:|:.:
  Fly    93 TQAEKTKFLWNLYCNDAGTFIIKS------------DYVRNVNL--------------------- 124

Mouse   355 LNEVNKVISSVSYY--THRHGSSEDEEWLTAER----MAEWIQQNNILSIVLRDSLHQPQYVEKL 413
                 ....|||.:  :.|....:.::|:...|    :::|:..:|.:|  |...|..|.:.:.|
  Fly   125 -----APFESVSLFAQSERVNFEQFQDWIIKHRNATVLSKWLLSDNCVS--LTSELETPTFYQSL 182

Mouse   414 EKILRFVIKEKALTLQDLD-NIW----AAQAGKHE----------AIVKNVHDLLAKLAWDFSPE 463
            ..:..  ::||  .:.||: ..|    .:|.|:.:          .|.||.   ||.|...|...
  Fly   183 AGVTH--LEEK--DIGDLEKEFWRLKNTSQNGQIDLQFLGPLISPPIPKNA---LAGLFNAFDEN 240

Mouse   464 QLDHLFDCFKASWTNASKKQREKLLELIR---RLAEDDKDGVMAH----KVLNLLWNLAHSDDVP 521
            :..|:  .||......|...|...:|..|   ::.:.|:|||::|    :::|:|..:|..:...
  Fly   241 RDGHI--DFKELCCGVSAACRGPGVERTRFCFKIFDVDRDGVLSHDETLQMINVLLLVAKENQES 303

Mouse   522 VDIMDLALSAHIK-ILDYSCSQD---------RDTQKIQWIDRFIEELRTNDKWVIPALKQIREI 576
            ....||.....|. :|::...:.         ||...:...|..:..::.:.:.:.|.|..|.|:
  Fly   304 QQYKDLTKQLVISDLLEFGQRRSPDGTPSKLTRDNVSLTAEDFMLWTVQCDLRLMQPLLDLIFEL 368

Mouse   577 CSL-FGEAPQ------NLSSSRFSQSQRSPHVFYR---------HD-LINQLQHNHALV------ 618
            |.: ||..||      ::......:.:|.|   ||         || .::.:|:.....
  Fly   369 CHIVFGLWPQCKHMENDIVRGWLRREERRP---YRVGQFWYLITHDWWLSWMQYTQHTTHTCDYC 430

Mouse   619 -------TLVAENLATYMESMRMYGRDNEDYDPQTVRLGS------------------RY----- 653
                   |.|.|.|.. .||...:..:..|    :..|||                  |:
  Fly   431 KRTASQRTAVDEALVC-DESFNTHSLEQHD----SYSLGSGTGSASGSGSASSGISAGRHCGPVR 490

Mouse   654 ------SHVQEVQERLNFLRFLLKDGQLWLCAPQA---------KQIWKCLAENAVYLCDREACF 703
                  |::.......|......:.|.|....|..         |.:||.|.
  Fly   491 PGPIDNSNLITANPFRNVRTLTGEGGHLKRDTPLVQNHDFELVPKSLWKALN------------- 542

Mouse   704 KWYSKLMGDEPDL--------DPDINKDFFESNVL----QLDPS--------------------- 735
            :||    ||...|        :.|:..:.:..|:.    |..||
  Fly   543 RWY----GDNLPLPRQVIQPPNSDVELELYPLNLRILLHQAQPSQTGVGGGTQLGSWGSTVSGGY 603

Mouse   736 -LLTENG----------MKCFERFFKAVNCREGKLVAKRRA--YMMDDLELIGLDY-LWRVVIQS 786
             :|...|          ::..:|:. |......:|...|:.  ::.:.|.|...|. ||.|
  Fly   604 GVLASGGGYAAIAVSSVLQPPKRYL-AYTAAFSRLATVRQVGEFLCEQLRLKSEDIRLWHV---- 663

Mouse   787 NDDIACRAIDLLKEIYTNLGPRLQVNQVVIHEDFIQSCFDRLKASYDTLCVLDGDKDSINCARQE 851
                                |:|....:::.||.:  |...       |.:.|.|:
  Fly   664 --------------------PQLDNGAILLEEDAM--CLKE-------LLIRDNDQ--------- 690

Mouse   852 AVRMVRVLTVLREYINECDSDYHEERTILPMSRAFRGKHLSFIVRFPNQGRQVDDLEVWSHTNDT 916
                     :|.|..|: |..:.||...|..::..:|      ...|...|::        |..:
  Fly   691 ---------LLLEIRNK-DLTWPEELGSLATAQCGQG------AGTPGDRRRL--------TRSS 731

Mouse   917 IGSV------------RRCILN---------------------RIKANVAH---TKIELFVGGEL 945
            |.||            ..|.:|                     |.:.|.|:   ||
  Fly   732 IMSVHAPGATGLHNLGNTCFMNAALQVLFNTQPLAQYFQREMHRFEVNAANKLGTK--------- 787

Mouse   946 IDPGDDRKLIGQLNLKDKSLITAKLTQISSNMPSSPDSSSDSSTGSP-------GNHGNHYSDGP 1003
                      |||.::...|:....|..:.::             :|       ..:...::.|.
  Fly   788 ----------GQLAMRYAELLKEVWTATTRSV-------------APLKLRFCVNKYAPQFAGGG 829

Mouse  1004 NPEVESCLPGVIMSLHPRYISFLWQVADLGSSLNMP--PLRD-GARVLMKLMPPDSTTIEKLRAI 1065
            ..:.:..|..::.:||          .||...:..|  .|:| ..|       ||.  |....|.
  Fly   830 QHDSQELLEWLLDALH----------EDLNRVMEKPYSELKDSNGR-------PDK--IVAAEAW 875

Mouse  1066 CLDHAKLGESSLSPSLDSLFFGPSASQVLYL---TEVV----YALLMPAGAPLTDDSSDFQFHF- 1122
            ...||:    :.|..:| ||:|...|:|..|   .|.|    ::||   ..||..::   ..:|
  Fly   876 SQHHAR----NQSIIID-LFYGQLKSKVSCLGCGHESVRFDPFSLL---SLPLPVEN---YIYFE 929

Mouse  1123 ----LKSGGLPLVLSMLTRNNFLPNADMETRRGAYLNALKIAKLLLTAIGYGHVRAVAEACQPGV 1183
                |..|.:|:      :..|..|:|.:                     |.|::
  Fly   930 VLVILLDGSVPI------KYGFRLNSDCK---------------------YSHLK---------- 957

Mouse  1184 EGVNPMTSVNQVTHDQAVVLQSALQSIPNPSSECMLRNVSVRLAQQISDEASRYMPDICVIRAIQ 1248
            ..::.|.|:.                 ||....|.|.|..:|  |.::|:..
  Fly   958 HKLSTMCSLP-----------------PNLMLVCELWNSQIR--QVLNDDEK------------- 990

Mouse  1249 KIIWTSGCGGLQLVFSPNEEVTKIYEKTNAGNEPDLEDEQVCCEALEVMTLCFALIPTALDALSK 1313
              :.|.....|.:...|.:.:.   .::|:|....:|      :.|:.:....|||.:|.|:||.
  Fly   991 --LRTQSAKELYVYQLPEQSMR---TRSNSGLSMHIE------QGLKDIQRSSALITSAQDSLSS 1044

Mouse  1314 EKAWQTFIIDLLLHCHSKTVRQVAQEQFFLMCTRCCMGHRPLLFFITLLFTVLGSTARERAKHSG 1378
            ....||       ..|..:.|.:            |.||            |.|......|:...
  Fly  1045 LSTLQT-------SSHRASSRVL------------CNGH------------VSGLDVEGEAEVGT 1078

Mouse  1379 DYFTLLRHLLNYAYNSNINVPNAEVLLNNEIDWLKRIRDDVKRTGET-GVEETILEGHLGVTKEL 1442
            |   :.:...|..||..::..:.....:|::..|  :.|:..:.... |..|.:........:.|
  Fly  1079 D---VSQCNSNSNYNPIVSTYSGNGSGDNQVHEL--LPDEAGKVSRCFGKRECMPHSLFCFKESL 1138

Mouse  1443 LAFQTPEKKFHIGC-----EKGGANLIKELIDDFIFPASNVYLQYMRNGELPAEQAIPVCGSPAT 1502
            :...:||..|..|.     ....|.|:....:......:|      .:||...|
  Fly  1139 ILSSSPENTFMHGAAAQQKRVSSAKLLHTESNTSSMSYTN------HSGENSME----------- 1186

Mouse  1503 INAGFELLVALAVGCVRNLKQIVDSLTEMYYIGTAITTCEALTEWEYLPPVGPRPPKGFVGLKNA 1567
                                   .||||...:..             |.||..|...|
  Fly  1187 -----------------------SSLTEPIPLAD-------------LEPVSSRNGSG------- 1208

Mouse  1568 GATC-YMNSVIQQLYMIPSIRNGILAIEGTGSDVDDDMSGDEKQDNESNVDPRDDVFGYPQQFED 1631
            |..| |..|        |:..:|:......|:.:|.|     :|..|.|.:..|.    |.|...
  Fly  1209 GEDCSYRTS--------PNDSSGLSTGHTLGASLDVD-----EQAEEGNAEDHDQ----PDQITT 1256

Mouse  1632 KPP------LSKTEDRKEYNIGVLRHLQVIFGHLAASR-----LQYYVPRGFWKQFRLWGEPVNL 1685
            ..|      .|:...:..:..|  ::|..:  |...:|     |.|:..|.     .|:|.|:.:
  Fly  1257 SQPETSSGVYSRRSSQPPHKAG--KYLVAV--HRKITRHDSYFLSYHKTRP-----SLFGVPLLI 1312

Mouse  1686 --------REQHDALEF-FNSLVDSLDEALKALGHPAMLSKVLGGSF--------AD-------- 1725
                    ::.:.|:.. .:.|:..|....:...|.|.....||..|        ||
  Fly  1313 PNSEGGTHKDLYCAVWLQVSRLLSPLPATTEQANHAADCDDSLGYDFPFTLRAVKADGLTCAICP 1377

Mouse  1726 -QKICQGCPHR-------------------------------------------YECEESFT--- 1743
             ...|:||..|                                           |....|:|
  Fly  1378 WSSFCRGCEIRCNNDYVLQGALPPINAAASNTSTPKMNAKFPSLPNLEAKRTPEYTASLSYTPTT 1442

Mouse  1744 ------TLNVD-------IRNHQNLLDSL------------EQYVKGDLLEGANA---------- 1773
                  |:.:|       :| :|:.|:.|            ||....||.....|
  Fly  1443 KYFEDFTIAIDWDPTALHLR-YQSTLERLWVDHETIAISRREQVEPVDLNHCLRAFTSEEKLEQW 1506

Mouse  1774 YHCEKCNKKVDTVKRLLIKKLPPVLAIQLKRF-------------------DYD-------WERE 1812
            |||..|..|....|:|.|.||||:|.:.||||                   |:|       ..:|
  Fly  1507 YHCSHCKGKKPATKKLQIWKLPPILIVHLKRFNCVNGKWVKSQKVVHFPFDDFDPTPYLASVPQE 1571

Mouse  1813 CAIKFNDYFEFPRELDMEPYTVAGVAKLEGDNVNPESQLIQQN--EQSESEKAGST--------- 1866
            ..::..:..|...:.:|...|...|::|  |.::..|:.:::.  .|:.|.||.::
  Fly  1572 TILRHKELLELKNDAEMTMATNEVVSEL--DEIDAPSKEVKEELPNQTGSTKATASPPPTGNILR 1634

Mouse  1867 ---------------------------------------------KYRLVGVLVHSGQASGGHYY 1886
                                                         :|||..|:.|||..:||||.
  Fly  1635 QSKTKNAVRRQRLISTSLTKTPIVDGEFEDYHQHRLKPDVDQFDPRYRLYAVVSHSGMLNGGHYI 1699

Mouse  1887 SYIIQRNGGDGEKNRWYKFDDGDVTECKMDDDEEMKNQCFGGEYMGEVFDHMMKRMSYRRQKRWW 1951
            ||.....|.      ||.::|....|....                .|.|...
  Fly  1700 SYASNATGS------WYCYNDSSCREISQK----------------PVIDPSA------------ 1730

Mouse  1952 NAYILFYERMDTIGHDDEVIRYISEIAITTRPHQIVMPSAIERS 1995
             ||:|||||.   |.|.|  .|:..|...|.|:...:|..::.:
  Fly  1731 -AYLLFYERK---GLDYE--PYLPNIEGRTLPNTASVPLEVDET 1768

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Usp9x	XP_006527649.1	Ubl1_cv_Nsp3_N-like	<905..969	CDD:475130	15/99 (15%)
		peptidase_C19C	1560..1963	CDD:239124	114/603 (19%)
		DUF3517	2102..2481	CDD:463438
Usp32	NP_001262074.2	EFh_PI-PLC	198..>251	CDD:333715	13/57 (23%)
		EF-hand motif	198..222	CDD:320029	3/23 (13%)
		EF-hand_7	227..290	CDD:463900	18/67 (27%)
		UBP12	486..>1002	CDD:227847	117/732 (16%)
		Peptidase_C19	<1480..1737	CDD:470612	60/293 (20%)