Protein Alignment EPRS1 and GluProRS

DIOPT Version :10

• Sequence 1: NP_004437.2
• Gene: EPRS1 / 2058
• HGNCID: 3418
• Length: 1512
• Species: Homo sapiens
• Sequence 2: NP_524471.2
• Gene: GluProRS / 42834
• FlyBaseID: FBgn0005674
• Length: 1714
• Species: Drosophila melanogaster
• Alignment Length: 1728
• Identity: 873/1728 - (50%)
• Similarity: 1097/1728 - (63%)
• Gaps: 233/1728 - (13%)

Human     3 TLSLTVNSGDPPLGALLAVEHVKDDVSISVEEGKENI-LHVSEN--VIFTDVNSILRYLARVATT 64
            ::.|..|..:||:..|.....:...|.:.:...||.. |...:|  ::....|.:||.|||.|..
  Fly     2 SIKLKANLNNPPISGLATAHLINGTVPVEIVWSKEETSLQFPDNRLLVCHSNNDVLRALARAAPD 66

Human    65 AGLYGSNLMEHTEIDHWLEFSATKLSSC-DSFTSTINELNHCLSLRTYLVGNSLSLADLCVWATL 128
            ..|||...:|.|:|||||.||.|    | |..:..::.|:..::..||||.|.|::||..::..:
  Fly    67 YKLYGETAIERTQIDHWLSFSLT----CEDDISWALSFLDKSIAPVTYLVANKLTIADFALFNEM 127

Human   129 KGNAAWQEQLKQKKAPVHVKRWFGFLEAQQAFQSV--------GTKWDVSTTKARV---APEKKQ 182
            ...   .|.|..|..|.||:||:..:.||...|.|        ..|....::|.:.   ..|:||
  Fly   128 HSR---YEFLAAKGIPQHVQRWYDLITAQPLIQKVLQSLPEDAKVKRSPQSSKEQTPAKTGERKQ 189

Human   183 DVGKFVELPGAEMGKVTVRFPPEASGYLHIGHAKAALLNQHYQVNFKGKLIMRFDDTNPEKEKED 247
            : ||||:|||||||||.|||||||||||||||||||||||:|.:.|:|.||||||||||.||..:
  Fly   190 E-GKFVDLPGAEMGKVVVRFPPEASGYLHIGHAKAALLNQYYALAFQGTLIMRFDDTNPAKETVE 253

Human   248 FEKVILEDVAMLHIKPDQFTYTSDHFETIMKYAEKLIQEGKAYVDDTPAEQMKAEREQRIDSKHR 312
            ||.|||.|:..|.||||.||:||::|:.::.|..:||:|.||||||||.||||.|||||::|.:|
  Fly   254 FENVILGDLEQLQIKPDVFTHTSNYFDLMLDYCVRLIKESKAYVDDTPPEQMKLEREQRVESANR 318

Human   313 KNPIEKNLQMWEEMKKGSQFGQSCCLRAKIDMSSNNGCMRDPTLYRCKIQPHPRTGNKYNVYPTY 377
            .|.:||||.:||||.|||:.||..|:||||||||.||||||||:||||.:||||||.||.|||||
  Fly   319 SNSVEKNLSLWEEMVKGSEKGQKYCVRAKIDMSSPNGCMRDPTIYRCKNEPHPRTGTKYKVYPTY 383

Human   378 DFACPIVDSIEGVTHALRTTEYHDRDEQFYWIIEALGIRKPYIWEYSRLNLNNTVLSKRKLTWFV 442
            ||||||||:||.|||.||||||||||:||||.|:||.:||||||.|||||:.|||||||||||||
  Fly   384 DFACPIVDAIENVTHTLRTTEYHDRDDQFYWFIDALKLRKPYIWSYSRLNMTNTVLSKRKLTWFV 448

Human   443 NEGLVDGWDDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKVIDPVAPRY 507
            :.||||||||||||||||::||||||||||:||.|||||:|||.|.||||||||||||||:||||
  Fly   449 DSGLVDGWDDPRFPTVRGIIRRGMTVEGLKEFIIAQGSSKSVVFMNWDKIWAFNKKVIDPIAPRY 513

Human   508 VALLKKEVIPVNVPEAQEEMKEVAKHPKNPEVGLKPVWYSPKVFIEGADAETFSEGEMVTFINWG 572
            .||.|::.:.|||..|:.|..:|:.|||:..:|.|.|...|:::|:..|||...|||..||||||
  Fly   514 TALEKEKRVIVNVAGAKVERIQVSVHPKDESLGKKTVLLGPRIYIDYVDAEALKEGENATFINWG 578

Human   573 NLNITKIHKNADGKIISLDAKLNLENKDYKKTTKVTWLA--ETTHALPIPVICVTYEHLITKPVL 635
            |:.|.|::|:|.|.|.|:||.|||||||:|||.|:||||  :...|.| |..||.::::|:|.||
  Fly   579 NILIRKVNKDASGNITSVDAALNLENKDFKKTLKLTWLAVEDDPSAYP-PTFCVYFDNIISKAVL 642

Human   636 GKDEDFKQYVNKNSKHEELMLGDPCLKDLKKGDIIQLQRRGFFICDQPYEPVSPYSCKEAPCVLI 700
            ||||||||::...::.|..|||||.||..||||||||||||||..|..|.|.|.|:...:|.||.
  Fly   643 GKDEDFKQFIGHKTRDEVPMLGDPELKKCKKGDIIQLQRRGFFKVDVAYAPPSGYTNVPSPIVLF 707

Human   701 YIPDGHTKEMPTSGSK-EKTKVEATKNETS----------------------------------- 729
            .|||||||::||||.| .....:|||..:|
  Fly   708 SIPDGHTKDVPTSGLKVNAPDAKATKKASSPVSSSGQASELDSQISQQGDLVRDLKSKKAAKDQI 772

Human   730 -----------APFK---------------ERPTPSLNNNCTTSEDSLVLYNRVAVQGDVVRELK 768
                       |.:|               ..|.|:.::  :::.|::.:...:..|||:||:||
  Fly   773 DVAVKKLLALKADYKSATGKDWKPGQTSASSAPVPAASS--SSANDAVSVNASIVKQGDLVRDLK 835

Human   769 AKKAPKEDVDAAVKQLLSLKAEYKEKTGQEYKPGNPPAEIGQNISSNSSASILES-KSLYDEVAA 832
            .|||.|.::|||||.||.|||:||..|||::|||..|.....:.|:..|..:.:| ..:..::.|
  Fly   836 GKKASKPEIDAAVKTLLELKAQYKTLTGQDWKPGTVPTTAAPSASAAPSVGVNDSVAQILSQITA 900

Human   833 QGEVVRKLKAEKSPKAKINEAVECLLSLKAQYKEKTGKEYIPG---QPPLSQSSDSSPTRNSEPA 894
            ||:.||:||:.|:.||.::.||:.||||||.||..||.::.||   ..|.:........:|.:||
  Fly   901 QGDKVRELKSAKADKATVDAAVKTLLSLKADYKAATGSDWKPGTTAPAPAAAPVKVKQEKNPDPA 965

Human   895 GLETPEAKVLFDKVASQGEVVRKLKTEKAPKDQVDIAVQELLQLKAQYKSLIGVEYKP------- 952
            .:.|  ...|.:|:|.||:.:|:||:.|:.|..|:..|:.||.||..||||.|.|:||
  Fly   966 SVLT--VNTLLNKIAQQGDKIRQLKSAKSEKSLVEAEVKLLLALKTDYKSLTGQEWKPGTVAPAP 1028

Human   953 -----VSATGAE----------------DKDKK-KKEK--------------------------- 968
                 :..||.:                ||.:| |.||
  Fly  1029 TTVNVIDLTGGDSGSDVGSVLSKIQAQGDKIRKLKSEKAAKNVIDPEVKTLLALKGEYKTLSGKD 1093

Human   969 ---ENKSEKQNKPQKQNDGQRKDPSKNQ------------------------------------- 993
               :.|||.....::.:......|:|::
  Fly  1094 WTPDAKSEPAVVKKEASPVSMASPAKDELTQEINAQGEKVRAAKGNKAAKEVIDAEVAKLLALKA 1158

Human   994 -------------------GGGLSSSGAGEGQ-----------------GPKKQTRLGLEAKKEE 1022
                               |||.:.....|.|                 ..||||||||||.||:
  Fly  1159 KYKEVTGTDFPVAGRGGGGGGGSAKKAPKEAQPKPAKPVKKEPAADASGAVKKQTRLGLEATKED 1223

Human  1023 NLADWYSQVITKSEMIEYHDISGCYILRPWAYAIWEAIKDFFDAEIKKLGVENCYFPMFVSQSAL 1087
            ||.|||||||||.|||||:|:|||||||.|::|||:|||.:|||||.::||:.||||:|||::.|
  Fly  1224 NLPDWYSQVITKGEMIEYYDVSGCYILRQWSFAIWKAIKTWFDAEITRMGVKECYFPIFVSKAVL 1288

Human  1088 EKEKTHVADFAPEVAWVTRSGKTELAEPIAIRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVR 1152
            ||||||:|||||||||||:||.::||||||:||||||||||||||||||:|||||:||||.||||
  Fly  1289 EKEKTHIADFAPEVAWVTKSGDSDLAEPIAVRPTSETVMYPAYAKWVQSYRDLPIRLNQWNNVVR 1353

Human  1153 WEFKHPQPFLRTREFLWQEGHSAFATMEEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKEKFA 1217
            ||||.|.||||||||||||||:|||..||||:|||.||||||.||..||||||||||||||||||
  Fly  1354 WEFKQPTPFLRTREFLWQEGHTAFADKEEAAKEVLDILDLYALVYTHLLAIPVVKGRKTEKEKFA 1418

Human  1218 GGDYTTTIEAFISASGRAIQGGTSHHLGQNFSKMFEIVFEDPKIPGEKQFAYQNSWGLTTRTIGV 1282
            |||||||:|||||||||||||.||||||||||||||||:|||:.. :|::.||||||:|||||||
  Fly  1419 GGDYTTTVEAFISASGRAIQGATSHHLGQNFSKMFEIVYEDPETQ-QKKYVYQNSWGITTRTIGV 1482

Human  1283 MTMVHGDNMGLVLPPRVACVQVVIIPCGITNALSEEDKEALIAKCNDYRRRLLSVNIRVRADLRD 1347
            |.|||.||.||||||.|||:|.:::|||||....::::..|:..|....:||:...:|...|.||
  Fly  1483 MIMVHADNQGLVLPPHVACIQAIVVPCGITVNTKDDERAQLLDACKALEKRLVGGGVRCEGDYRD 1547

Human  1348 NYSPGWKFNHWELKGVPIRLEVGPRDMKSCQFVAVRRDTGEKLTVAENEAETKLQAILEDIQVTL 1412
            |||||||||||||||||:||||||:|:|:.|.|||||||.||:|:...:.|.|:.|:||.|..::
  Fly  1548 NYSPGWKFNHWELKGVPLRLEVGPKDLKAQQLVAVRRDTVEKITIPLADVEKKIPALLETIHESM 1612

Human  1413 FTRASEDLKTHMVVANTMEDFQKILDSGKIVQIPFCGEIDCEDWIKKTTARDQDLEPGAPSMGAK 1477
            ..:|.||:.:|........||...|:...|:..||||||.|||.||..:||.::.|||||:||||
  Fly  1613 LNKAQEDMTSHTKKVTNWTDFCGFLEQKNILLAPFCGEISCEDKIKADSARGEEAEPGAPAMGAK 1677

Human  1478 SLCIPFKPLCELQPGAKCV---CGKNPAKYYTLFGRSY 1512
            ||||||.....:....||:   |...| |:||||||||
  Fly  1678 SLCIPFDQPAPIAASDKCINPSCTNKP-KFYTLFGRSY 1714

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
EPRS1	NP_004437.2	PLN02907	13..711	CDD:215492	411/714 (58%)
		Glutamate--tRNA ligase. /evidence=ECO:0000250|UniProtKB:P28668	164..759		374/661 (57%)
		'HIGH' region. /evidence=ECO:0000250|UniProtKB:P28668	204..214		9/9 (100%)
		'KMSKS' region. /evidence=ECO:0000250|UniProtKB:P28668	432..436		3/3 (100%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	709..744		13/96 (14%)
		WEPRS_RNA	753..802	CDD:238473	27/48 (56%)
		3 X 57 AA approximate repeats. /evidence=ECO:0000303|PubMed:1988429	760..956		86/211 (41%)
		WEPRS_RNA	826..875	CDD:238473	22/48 (46%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	869..900		8/33 (24%)
		WEPRS_RNA	904..952	CDD:238473	22/47 (47%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	956..1012		20/175 (11%)
		Proline--tRNA ligase. /evidence=ECO:0000250|UniProtKB:P28668	1007..1512		340/507 (67%)
		proS_fam_I	1018..1512	CDD:273062	331/496 (67%)
GluProRS	NP_524471.2	PLN02907	1..715	CDD:215492	411/721 (57%)
		WEPRS_RNA	748..797	CDD:238473	2/48 (4%)
		WHEP-TRS	821..870	CDD:459819	28/48 (58%)
		WEPRS_RNA	894..943	CDD:238473	22/48 (46%)
		WEPRS_RNA	973..1022	CDD:238473	23/48 (48%)
		WEPRS_RNA	1048..1097	CDD:238473	6/48 (13%)
		WEPRS_RNA	1122..1169	CDD:238473	0/46 (0%)
		proS_fam_I	1219..1714	CDD:273062	331/496 (67%)