DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment EPHB4 and Eph

DIOPT Version :9

Sequence 1:	XP_016867305.1	Gene:	EPHB4 / 2050	HGNCID:	3395	Length:	1005	Species:	Homo sapiens
Sequence 2:	NP_524625.3	Gene:	Eph / 43803	FlyBaseID:	FBgn0025936	Length:	1096	Species:	Drosophila melanogaster

Alignment Length:	1056	Identity:	422/1056 - (39%)
Similarity:	594/1056 - (56%)	Gaps:	123/1056 - (11%)

- Green bases have known domain annotations that are detailed below.

Human    12 LAAALEETLLNTKLETADLKWVTFP-----QVDGQWEELSGLD-EEQHSVRTYEVCDVQRAPGQA 70
            ||.|.:..||:|..| |.|:|..:|     |..| |.|.|..| .:..:.|:|.|||| ......
  Fly    79 LAHADQVVLLDTTRE-ATLEWTRYPYGPQAQTPG-WVEESFTDFVKGINWRSYVVCDV-AYHNVN 140

Human    71 HWLRTGWVPRRGAVHVYATLRFTMLECLSLPRAGRSCKETFTVFYYESDADTATALTPAWMENPY 135
            :||.:.::.|..|..:|..::||:.:|...|....||||||::.:||.||  ||...|.|..:.|
  Fly   141 NWLWSPFIDRGSANRLYIEIQFTIRDCSLFPGNALSCKETFSLLFYEFDA--ATREPPPWQTDSY 203

Human   136 IKVDTVAAEHLTRKRPGAEATGKVNVKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKCAQL 200
            ..:..:||.. .|....::......||::   .::|.|.|.||:|||||:::|::.::|..|..:
  Fly   204 RLIARIAAGE-GRFNQNSDVDINTEVKSI---AVNKKGVYFAFRDQGACISVLAVKVYYITCPAV 264

Human   201 TVNLTRFPET-VPRELVV--PVAGSCVVDAVPAPGPSPSLYCREDGQWAEQPVTGCSCAPGFEAA 262
            |.|...|.|| ..||:.:  ...|:||.:|  .|..:|:..|:.||:|... ..||.|..|:|..
  Fly   265 TENFAHFNETPTGREITIIEKQNGTCVDNA--EPYETPTYLCKGDGKWTIL-TGGCRCKAGYEPN 326

Human   263 EGNTKCRACAQGTFKPLSGE-GSCQPCPANSHSNTIGSAVCQCRVGYFRARTDPRGAPCTTPPSA 326
            ..|..|..|..||||  |.| ..|.|||.||:::..||..|:|..|::|...|.|..||.:||:|
  Fly   327 YTNKTCTECPLGTFK--SPEVTKCTPCPPNSNASKTGSPFCKCASGFYRHPNDGRHMPCYSPPAA 389

Human   327 PRSV-VSRLNGSSLHLEWSAPL--ESGGRE--------DLTYALRCRECRPGGSCAPCGGDLTFD 380
            |.:: :..::.:|..:.||||.  ||...|        |:.|.::|..|.|         ::.::
  Fly   390 PTNLTLLFVDQTSAIISWSAPAKNESFSSETNSKIYHSDIVYKIKCNICSP---------NVVYN 445

Human   381 PGPRDLVEPWVVVRGLRPDFTYTFEVTALNGVSSL-------------ATGPVPFEP---VNVTT 429
            |......|..:.:..|.|..|||.::.|:|.||.:             |...:.|..   :|:..
  Fly   446 PSTDTFNETKITLTNLEPVTTYTVQIHAINSVSHINEFKRHSNESSLVAVSDIVFSNTSLLNIPL 510

Human   430 D-REVK-GQS---YHAQGFPVSLPLTVPPAVSDIRVTRSSPSSLSLAWAVPRAPSGAVLDYEVKY 489
            | .||| ||:   :..:...:|       .|.::|:...:.....|.|..|......:..|||::
  Fly   511 DLNEVKTGQAEIVFTTESVLLS-------TVFNLRILAITNKDADLEWDKPVQSDFPLEFYEVRW 568

Human   490 HEKGAEGPSSVRFLKTSENRAELRGLKRGASYLVQVRARSEAGYGPFGQEHHSQT------QLDE 548
            ..|......:...|.|.|.:|.:.||.....|..|||.::..|:|.:....::||      ..|:
  Fly   569 FPKVELDAINKSALNTKETKAHIVGLLENTEYGFQVRCKTNNGFGSYSNMIYAQTLQSVGSVYDD 633

Human   549 SEGWREQLALIAGTAVVGVVLVLVVIVVAVLCLRKQSNGREAEYSDKH----------------- 596
            |    .|:..|||..|.||:.:::.|:..|..:|.:......:.|..|
  Fly   634 S----VQIRFIAGAIVTGVLFLVIFIIATVYFMRSKHQDDLDKKSTNHLPLPLDYASNEVNSMDT 694

Human   597 -----------GQYLIGHGTKVYIDPFTYEDPNEAVREFAKEIDVSYVKIEEVIGAGEFGEVCRG 650
                       ...|.|: ::.|:||.||||||:|:||||:|||.:|:.||.:||.||||:||||
  Fly   695 TPIVKKLHLNVTTPLFGN-SRSYVDPHTYEDPNQAIREFAREIDANYITIEAIIGGGEFGDVCRG 758

Human   651 RLKAPGK--KESCVAIKTLKGGYTERQRREFLSEASIMGQFEHPNIIRLEGVVTNSMPVMILTEF 713
            |||.|..  ::..|||||||.|.:|:.|.:||:||||||||:|||:|.|:||||.|.||||:||:
  Fly   759 RLKIPPNFVQDIDVAIKTLKPGSSEKARCDFLTEASIMGQFDHPNVIYLQGVVTRSNPVMIITEY 823

Human   714 MENGALDSFLRLNDGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNSNLVCKVSDFG 778
            ||||:||:|||:|||:|..:||:.|||||||||.||::|:||||||||||:|||:.|:||::|||
  Fly   824 MENGSLDTFLRVNDGKFQTLQLIVMLRGIASGMSYLSDMNYVHRDLAARNVLVNAQLICKIADFG 888

Human   779 LSRFLEENSSDPTYTSSLGGKIPIRWTAPEAIAFRKFTSASDAWSYGIVMWEVMSFGERPYWDMS 843
            |||.: ||:|| .||:. |||||:||||||||||||||||||.||||:|:|||||:||||||:.|
  Fly   889 LSREI-ENASD-AYTTR-GGKIPVRWTAPEAIAFRKFTSASDVWSYGVVLWEVMSYGERPYWNWS 950

Human   844 NQDVINAIEQDYRLPPPPDCPTSLHQLMLDCWQKDRNARPRFPQVVSALDKMIRNPASLKIVAR- 907
            |||||.:||:.||||.|.|||.:|:|||||||||.|..||.|..:||.||.:.|.|.|| :..|
  Fly   951 NQDVIKSIEKGYRLPAPMDCPEALYQLMLDCWQKQRTHRPTFASIVSTLDNLARQPQSL-LTTRP 1014

Human   908 --ENGGASHPLLDQRQPHYSAFGSVGEWLRAIKMGRYEESFAAAGFGSFELVSQISAEDLLRIGV 970
              |:.| :|.|..||  ..:.|.|...||..|||.||...|..|...:.:.:|:::|:.|..:|:
  Fly  1015 SPESDG-NHILDGQR--GQNIFISTDLWLEHIKMSRYCHHFKEANLINAQQISRLTAQQLSDMGI 1076

Human   971 TLAGHQKKILASVQHM 986
            ||.|||||||...:.:
  Fly  1077 TLVGHQKKILHQARQL 1092

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
EPHB4	XP_016867305.1	EphR_LBD_B4	17..196	CDD:198442	61/184 (33%)
		FN3	324..429	CDD:238020	30/131 (23%)
		FN3	452..544	CDD:238020	21/91 (23%)
		EphA2_TM	<582..628	CDD:291255	19/73 (26%)
		PTKc_EphR_B	628..896	CDD:173638	183/269 (68%)
		Pkinase_Tyr	633..892	CDD:285015	178/260 (68%)
		SAM	922..989	CDD:197735	24/65 (37%)
		SAM_EPH-B4	925..991	CDD:188953	24/62 (39%)
Eph	NP_524625.3	EphR_LBD	85..260	CDD:198439	61/183 (33%)
		FN3	387..476	CDD:238020	24/97 (25%)
		FN3	536..623	CDD:238020	20/86 (23%)
		EphA2_TM	644..736	CDD:291255	24/92 (26%)
		PTKc_EphR	736..1002	CDD:270629	183/268 (68%)
		Pkinase_Tyr	741..999	CDD:285015	178/260 (68%)
		SAM_1	1033..1092	CDD:278937	24/58 (41%)
		SAM	1039..1092	CDD:197735	22/52 (42%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	399	1.000	Domainoid score	I741
eggNOG	1	0.900	-	-		E2759_KOG0196
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	762	1.000	Inparanoid score	I548
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG45908
OrthoDB	1	1.010	-	-		D397694at33208
OrthoFinder	1	1.000	-	-		FOG0000086
OrthoInspector	1	1.000	-	-		otm42229
orthoMCL	1	0.900	-	-		OOG6_100189
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X82
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	12	11.740