DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment EPHB2 and Eph

DIOPT Version :9

Sequence 1:	NP_001296122.1	Gene:	EPHB2 / 2048	HGNCID:	3393	Length:	1055	Species:	Homo sapiens
Sequence 2:	NP_524625.3	Gene:	Eph / 43803	FlyBaseID:	FBgn0025936	Length:	1096	Species:	Drosophila melanogaster

Alignment Length:	1084	Identity:	450/1084 - (41%)
Similarity:	618/1084 - (57%)	Gaps:	150/1084 - (13%)

- Green bases have known domain annotations that are detailed below.

Human     1 MALRRLGAALLLLPL-----LAAVEETLMDSTTATAELGWMVHP------PSGWEEVSGYD--EN 52
            |:|.|   .:||:.:     ||..::.::..||..|.|.|..:|      ..||.|.|..|  :.
  Fly    62 MSLLR---TILLIIISIHFKLAHADQVVLLDTTREATLEWTRYPYGPQAQTPGWVEESFTDFVKG 123

Human    53 MNTIRTYQVCNVFESSQNNWLRTKFIRRRGAHRIHVEMKFSVRDCSSIPSVPGSCKETFNLYYYE 117
            :|. |:|.||:|...:.||||.:.||.|..|:|:::|::|::||||..|....||||||:|.:||
  Fly   124 INW-RSYVVCDVAYHNVNNWLWSPFIDRGSANRLYIEIQFTIRDCSLFPGNALSCKETFSLLFYE 187

Human   118 ADFDSATKTFPNWMENPWVKVDTIAADES-FSQ---VDLGGRVMKINTEVRSFGPVSRSGFYLAF 178
              ||:||:..|.|..:.:..:..|||.|. |:|   ||       |||||:|.. |::.|.|.||
  Fly   188 --FDAATREPPPWQTDSYRLIARIAAGEGRFNQNSDVD-------INTEVKSIA-VNKKGVYFAF 242

Human   179 QDYGGCMSLIAVRVFYRKCPRIIQNGAIFQETLSGAESTSLVAARGSCIANAEEVDVPIKLYCNG 243
            :|.|.|:|::||:|:|..||.:.:|.|.|.||.:|.|.|.:....|:|:.|||..:.|..| |.|
  Fly   243 RDQGACISVLAVKVYYITCPAVTENFAHFNETPTGREITIIEKQNGTCVDNAEPYETPTYL-CKG 306

Human   244 DGEWLVPIGRCMCKAGFEAVENGTVCRGCPSGTFKANQGDEACTHCPINSRTTSEGATNCVCRNG 308
            ||:|.:..|.|.||||:|.......|..||.||||:.:..: ||.||.||..:..|:..|.|.:|
  Fly   307 DGKWTILTGGCRCKAGYEPNYTNKTCTECPLGTFKSPEVTK-CTPCPPNSNASKTGSPFCKCASG 370

Human   309 YYRADLDPLDMPCTTIPSAP-QAVISSVNETSLMLEWTPP--RDSGGRE--------DLVYNIIC 362
            :||...|...|||.:.|:|| ...:..|::||.::.|:.|  .:|...|        |:||.|
  Fly   371 FYRHPNDGRHMPCYSPPAAPTNLTLLFVDQTSAIISWSAPAKNESFSSETNSKIYHSDIVYKI-- 433

Human   363 KSCGSGRGACTRCGDNVQYAPRQLGLTEPRIYISDLLAHTQYTFEIQAVNGVTDQSPFSPQF--- 424
                    .|..|..||.|.|......|.:|.:::|...|.||.:|.|:|.|:..:.|....
  Fly   434 --------KCNICSPNVVYNPSTDTFNETKITLTNLEPVTTYTVQIHAINSVSHINEFKRHSNES 490

Human   425 -----------------------------ASVNITTNQAAPSAVSIMHQVSRTVDSITLSWSQPD 460
                                         |.:..||.....|.|..:..::.|.....|.|.:|.
  Fly   491 SLVAVSDIVFSNTSLLNIPLDLNEVKTGQAEIVFTTESVLLSTVFNLRILAITNKDADLEWDKPV 555

Human   461 QPNGVILDYELQYYEK-ELSEYNATAIKSPTNTVTVQGLKAGAIYVFQVRARTVAGYGRYSGKMY 524
            |.:..:..||::::.| ||...|.:|:.:......:.||.....|.||||.:|..|:|.||..:|
  Fly   556 QSDFPLEFYEVRWFPKVELDAINKSALNTKETKAHIVGLLENTEYGFQVRCKTNNGFGSYSNMIY 620

Human   525 FQTMTE--AEYQTSIQEKLPLIIGSSAAGLVFLIAVVVIAIVCNRRGFERADSEYTDKLQHYTSG 587
            .||:..  :.|..|:|  :..|.|:...|::||:..::..:...|       |::.|.|...::.
  Fly   621 AQTLQSVGSVYDDSVQ--IRFIAGAIVTGVLFLVIFIIATVYFMR-------SKHQDDLDKKSTN 676

Human   588 HM----------------TPGMK---------------IYIDPFTYEDPNEAVREFAKEIDISCV 621
            |:                ||.:|               .|:||.||||||:|:||||:|||.:.:
  Fly   677 HLPLPLDYASNEVNSMDTTPIVKKLHLNVTTPLFGNSRSYVDPHTYEDPNQAIREFAREIDANYI 741

Human   622 KIEQVIGAGEFGEVCSGHLKLPGK--REIFVAIKTLKSGYTEKQRRDFLSEASIMGQFDHPNVIH 684
            .||.:||.||||:||.|.||:|..  ::|.|||||||.|.:||.|.|||:||||||||||||||:
  Fly   742 TIEAIIGGGEFGDVCRGRLKIPPNFVQDIDVAIKTLKPGSSEKARCDFLTEASIMGQFDHPNVIY 806

Human   685 LEGVVTKSTPVMIITEFMENGSLDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLADMNYVHRDLAA 749
            |:||||:|.|||||||:|||||||:|||.|||:|..:||:.||||||:||.||:|||||||||||
  Fly   807 LQGVVTRSNPVMIITEYMENGSLDTFLRVNDGKFQTLQLIVMLRGIASGMSYLSDMNYVHRDLAA 871

Human   750 RNILVNSNLVCKVSDFGLSRFLEDDTSDPTYTSALGGKIPIRWTAPEAIQYRKFTSASDVWSYGI 814
            ||:|||:.|:||::||||||.:| :.|| .||:. |||||:||||||||.:|||||||||||||:
  Fly   872 RNVLVNAQLICKIADFGLSREIE-NASD-AYTTR-GGKIPVRWTAPEAIAFRKFTSASDVWSYGV 933

Human   815 VMWEVMSYGERPYWDMTNQDVINAIEQDYRLPPPMDCPSALHQLMLDCWQKDRNHRPKFGQIVNT 879
            |:||||||||||||:.:|||||.:||:.||||.|||||.||:|||||||||.|.|||.|..||:|
  Fly   934 VLWEVMSYGERPYWNWSNQDVIKSIEKGYRLPAPMDCPEALYQLMLDCWQKQRTHRPTFASIVST 998

Human   880 LDKMIRNPNSLKAMAPLSSGINLPLLD----RTIPDYTSFNTVDEWLEAIKMGQYKESFANAGFT 940
            ||.:.|.|.||....|........:||    :.|     |.:.|.|||.|||.:|...|..|...
  Fly   999 LDNLARQPQSLLTTRPSPESDGNHILDGQRGQNI-----FISTDLWLEHIKMSRYCHHFKEANLI 1058

Human   941 SFDVVSQMMMEDILRVGVTLAGHQKKILNSIQVMRAQMNQIQSV 984
            :...:|::..:.:..:|:||.|||||||:       |..|:.::
  Fly  1059 NAQQISRLTAQQLSDMGITLVGHQKKILH-------QARQLDTI 1095

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
EPHB2	NP_001296122.1	EphR_LBD_B2	19..196	CDD:198445	75/188 (40%)
		fn3	326..421	CDD:278470	29/105 (28%)
		fn3	437..520	CDD:278470	24/83 (29%)
		EphA2_TM	548..616	CDD:291255	25/98 (26%)
		PTKc_EphR_B	616..884	CDD:173638	190/269 (71%)
		Pkinase_Tyr	621..880	CDD:285015	185/260 (71%)
		SAM_EPH-B2	910..980	CDD:188951	23/69 (33%)
		SAM	910..977	CDD:197735	22/66 (33%)
		PDZ-binding (in isoform 2). /evidence=ECO:0000255	984..986		0/1 (0%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	990..1055
Eph	NP_524625.3	EphR_LBD	85..260	CDD:198439	75/185 (41%)
		FN3	387..476	CDD:238020	28/98 (29%)
		FN3	536..623	CDD:238020	25/86 (29%)
		EphA2_TM	644..736	CDD:291255	25/98 (26%)
		PTKc_EphR	736..1002	CDD:270629	190/268 (71%)
		Pkinase_Tyr	741..999	CDD:285015	185/260 (71%)
		SAM_1	1033..1092	CDD:278937	23/65 (35%)
		SAM	1039..1092	CDD:197735	21/59 (36%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	399	1.000	Domainoid score	I741
eggNOG	1	0.900	-	-		E2759_KOG0196
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	762	1.000	Inparanoid score	I548
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG45908
OrthoDB	1	1.010	-	-		D397694at33208
OrthoFinder	1	1.000	-	-		FOG0000086
OrthoInspector	1	1.000	-	-		otm42229
orthoMCL	1	0.900	-	-		OOG6_100189
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2895
SonicParanoid	1	1.000	-	-		X82
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	13	12.770