Protein Alignment EPHA4 and vab-1

DIOPT Version :9

• Sequence 1: NP_001291465.1
• Gene: EPHA4 / 2043
• HGNCID: 3388
• Length: 986
• Species: Homo sapiens
• Sequence 2: NP_494807.1
• Gene: vab-1 / 173794
• WormBaseID: WBGene00006868
• Length: 1117
• Species: Caenorhabditis elegans
• Alignment Length: 1100
• Identity: 358/1100 - (32%)
• Similarity: 547/1100 - (49%)
• Gaps: 194/1100 - (17%)

Human     6 YFALFSCLFGICDAVTGSRVYPANEVTLLDSRSVQGELGWIASPL---------EGGWEEVSIMD 61
            :..:..||..|   ||      :::..|.|...|..:|.|....|         |..|...:..|
 Worm    16 FILVLQCLITI---VT------SHQEVLFDLSKVGSDLKWDQVSLRHDRDDVWMEETWRNPAATD 71

Human    62 EKNTPIRTYQVCNVMEPSQNNWLRTDWITREGAQRVYIEIKFTLRDCNSLPGVMGTCKETFNLYY 126
            ||:...|.|..||....:.:|||.:.:|..:.|:|:|||:.|..|||::..... :|||||::|.
 Worm    72 EKHANQRAYVTCNYDMINPSNWLFSHFIEVKTARRIYIELLFNTRDCDAYLNPK-SCKETFSVYL 135

Human   127 YE--------SDNDKERFIRE-NQFVKIDTIA-ADESFTQVDIGDRIMKLNTEIRDVGPLSKKGF 181
            .:        :..:||||..: :.:..|..:| ::.:.|...:|   |:::::.:.:
 Worm   136 KQFKTSRPGSTKIEKERFSEDIDNWKNIGRLARSNSNMTTETLG---MEIDSDTKTI-------- 189

Human   182 YLAFQDVGACIALVSVRVFYKKCPLTVRNLAQFPDTITGADTSSLVEVRGSCVNNSEEKDVPKM- 245
            .:||::.|.|::|::|:::|:.|......|..|...:||...:.:|.:.|||:.|:.:| :|.:
 Worm   190 RIAFEEQGICLSLLNVKIYYRICDEFTDQLVYFRPQVTGPKETDMVRMNGSCIPNASKK-IPGVD 253

Human   246 ---YCGADGEWLVPIGNCLCNAGH----EERSGECQACKIGYYKALSTDATCAKCPPHSYSVWEG 303
               .|.:.|..:...|.|:|::|:    :.....|::|....||. ...:.|..||.:|.|. |.
 Worm   254 LIGLCMSTGSGIKTSGECVCDSGYSQIADSNGARCESCPTNTYKP-KGQSLCKSCPSNSISS-EA 316

Human   304 ATSCTCDRGFFRADNDAASMPCTRPPSAPLNLISN-VNETSVNLEWSSPQNTGGRQDISYNVVCK 367
            |:||.|..|:|||:::..|||||:|||.|:.|::| :..||..|.|:.|.:.|||.:|.|.|.|.
 Worm   317 ASSCRCLNGYFRAEDELISMPCTQPPSRPIKLVANAITATSTRLSWNEPSSLGGRPEIWYEVKCS 381

Human   368 KCGAGDPSKCRPCGSGVHYTPQQNGLKTTKVSITDLLAHTNYTFEIWAVNGVSKYNPN-PDQSVS 431
              |.|:      ||: |..||....|.|..|.|..|...::|||.::|.|.||...|. .:::..
 Worm   382 --GRGE------CGT-VVMTPGDKKLSTRSVQINGLRPSSDYTFLVFAKNKVSAQFPEFSEKNAV 437

Human   432 VTVTT----NQAAPSSIALVQAKEVTRYSVALAWLEPDRPNGVILEYEVKYYEKDQNERSYRIVR 492
            :.:.|    ....|.|...|.|.:..  .:.:||...|..   :.::||:.......:|::....
 Worm   438 IDIRTRSEEEDVPPVSHLRVDASQSD--GITIAWSVSDSD---VSDFEVEVRPAIVKKRTFETRH 497

Human   493 TAARNTDIKGLNPLTSYVFHVRART----------AAGYGDFSEPLEVTTNTV-PSRIIGDGANS 546
            .....|...||||.|.|.|.||.|.          ..|.|..|.|   ::|.| .|:.:....::
 Worm   498 VNMTYTTFIGLNPETVYQFRVRIRDDLRWSQPISYQLGRGLMSSP---SSNEVEESQFLNQTGSA 559

Human   547 TVLLVSVSGSVVLVVILIA-AFVISRRRSKYSKAKQEAD-----------------EEKHLNQG- 592
            .::::    :::|:||.:| ..::.:::||..|...:.|                 ...|.:||
 Worm   560 LLIII----ALILIVIAVALCMIVVQKKSKNRKQMSDLDVLDTYKQDSMTPDYHTTSRHHHHQGN 620

Human   593 -------------------------------------------VRTYVDPFTYEDPNQAVREFAK 614
                                                       .:|||||.|||||.||:.||..
 Worm   621 LPATLHEQLRSTTKLNAPLIPSFGSPISQPPPYYGGVHPNSGKYKTYVDPTTYEDPYQALIEFTF 685

Human   615 EIDASCIKIEKVIGVGEFGEVCSGRLK----VPGKREI--------------------CVAIKTL 655
            :|..:.:.|.:|||.||||:||.|.|.    ...|..:                    .||||||
 Worm   686 DISPNDVFITQVIGGGEFGDVCLGGLSKNSPAAAKWSVSNTTMGRGGGGGGYESEPYETVAIKTL 750

Human   656 KAGYTDKQRRDFLSEASIMGQFDHPNIIHLEGVVTKCKPVMIITEYMENGSLDAFLRKNDGRFTV 720
            |:|.:.|.:.:||:||:|||||.|||:|.|.||||..:||||:.|||.|||||.|||..|.|...
 Worm   751 KSGSSAKAKAEFLTEATIMGQFSHPNVIRLIGVVTSAEPVMIVAEYMANGSLDQFLRNTDQRGEK 815

Human   721 I---QLVGMLRGIGSGMKYLSDMSYVHRDLAARNILVNSNLVCKVSDFGMSRVLEDD---PEAAY 779
            :   ::..||.||.||||||:||.||||||||||:|::..|.||::|||:||.:..:   .|..|
 Worm   816 VHWEKITEMLYGIASGMKYLTDMGYVHRDLAARNVLLDMELRCKIADFGLSRGVRSEGSSVEPEY 880

Human   780 TTRGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVIKAIEEGYRLP 844
            ||.|||||:||||||||.:||||.:|||||:|:|:|||.|:|||||||.:||.||..:..|||||
 Worm   881 TTNGGKIPVRWTAPEAITHRKFTPSSDVWSFGVVIWEVCSFGERPYWDWTNQKVISEVMIGYRLP 945

Human   845 PPMDCPIALHQLMLDCWQKERSDRPKFGQIVNMLDKLI-------RNPNSLKRTGTESSRPNT-- 900
            ||||||:.|:::...||:.||.:||.|.|::....|.|       .:|..|.|.....|..||
 Worm   946 PPMDCPMGLYRIAQWCWKMERHERPTFTQLLATFHKYILQPTLIEHDPGELPRRVQSQSALNTYG 1010

Human   901 ---ALLDPSSPEFSA-VVSVGDWLQAIKMDRYKDNFTAAGYTTLEAVVHVNQEDLARIGI 956
               ..:.|:.|..:| :.|:.|:|:.|.::.......:....::..:.:.:..||...|:
 Worm  1011 SVNVGVVPTPPSSAAPMPSLDDFLRQIGLNHVYGQLVSNNIHSVSDLANTSHLDLLAYGL 1070

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
EPHA4	NP_001291465.1	EphR_LBD_A4	30..203	CDD:198450	50/191 (26%)
		FN3	329..436	CDD:238020	37/108 (34%)
		FN3	441..532	CDD:238020	25/100 (25%)
		EphA2_TM	562..618	CDD:405290	24/117 (21%)
		PTKc_EphR_A	616..882	CDD:270651	153/295 (52%)
		SAM_EPH-A4	911..981	CDD:188944	8/46 (17%)
		PDZ-binding. /evidence=ECO:0000255	984..986
vab-1	NP_494807.1	EphR_LBD	32..211	CDD:198439	50/190 (26%)
		FN3	342..423	CDD:238020	33/89 (37%)
		FN3	450..531	CDD:238020	21/85 (25%)
		EphA2_TM	<642..687	CDD:291255	14/44 (32%)
		PTKc_EphR	687..983	CDD:270629	153/295 (52%)
		STYKc	692..979	CDD:214568	151/286 (53%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	295	1.000	Domainoid score	I891
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H20933
Inparanoid	1	1.050	517	1.000	Inparanoid score	I753
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG45908
OrthoDB	1	1.010	-	-		D397694at33208
OrthoFinder	1	1.000	-	-		FOG0000086
OrthoInspector	1	1.000	-	-		otm16171
orthoMCL	1	0.900	-	-		OOG6_100189
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2895
SonicParanoid	1	1.000	-	-		X82
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	12	11.910