Protein Alignment EPHA1 and Tie

DIOPT Version :10

• Sequence 1: NP_005223.4
• Gene: EPHA1 / 2041
• HGNCID: 3385
• Length: 976
• Species: Homo sapiens
• Sequence 2: NP_523928.1
• Gene: Tie / 38559
• FlyBaseID: FBgn0014073
• Length: 1233
• Species: Drosophila melanogaster
• Alignment Length: 1261
• Identity: 248/1261 - (19%)
• Similarity: 387/1261 - (30%)
• Gaps: 527/1261 - (41%)

Human     5 WPLGLGLVLLLCAPLPPGARAKEVTLMDTSKAQGELGWLLDPPKDGWSEQQQI-----LNGT--- 61
            |.|.|.||.||.|.....|.|..::...|:...|.   :.:||.....:|||:     ||.|
  Fly    70 WLLLLLLVALLLAEAIVAAAAATLSETQTNATNGA---IREPPVQLQLQQQQLQLQLQLNATAGD 131

Human    62 -------PLYMYQD---------------------CPMQGRRDTDHWL------RSNWIYRGEEA 92
                   .::...|                     .|:...:|.:..|      |...::||...
  Fly   132 GESRNSAEIFSPGDVDVVGDKPTTYRPSGANRPSIAPLNSAQDRERKLMNVLAARRTALHRGGFR 196

Human    93 SRV------------------------------HVELQFT-------VRDCKSF-----PG---- 111
            :|:                              :....||       :|.|.:|     |.
  Fly   197 TRIGTTGRTAISPSAAVESTSKAPSDPRSVCIRNCTKNFTRRTTASCMRQCANFTRMAMPSNGNS 261

Human   112 -----------GAGPLGCKETFNLLYMESDQDVGIQLRRPLFQKVTTVAADQSFTIRDLVSGS-- 163
                       .|.|.|.::|..:|:  ||:.     ...||    .||.:|:.|:..:..|:
  Fly   262 SVVLKGSASLKAAAPAGDRDTNEILF--SDES-----SHGLF----VVAKEQNGTLNHIADGTKP 315

Human   164 --------VKLN-------VERCSLGRLTRRGLYLAFHNPGACVALVS------VRVFYQRCPET 207
                    .|.|       .|...|......|..|....||......:      .||......::
  Fly   316 AVMGKGIPSKTNSMEDDVEEEEDELKPYVYFGAKLPPIRPGGLTIKAAEGDDDHPRVLEVSVAQS 380

Human   208 LNGLAQFPDTLPGPAGLVE-VAGTCLP-------------HAR-------ASPRPSGAPRMHCSP 251
            .........|.|.|..... .|.|..|             |.|       |:|.|:..|    :|
  Fly   381 TTPTITTTSTTPAPVSSTRPTASTRRPLTPVERSRSKYKYHMRERGLVPTAAPPPAAPP----AP 441

Human   252 DGEWLVPVGRCHCEPGYEEGGSG----------------EACVA-----------CPSGSYRMDM 289
                  ||.|....      |||                |:.||           .||.|
  Fly   442 ------PVSRTRYV------GSGRPTAGLSDSIQDLKQTESEVAKPVVRRVVQKVSPSSS----- 489

Human   290 DTPHCL---------TCPQQSTAESEGATICTCESGHYRAPGEGPQVACTGPPSAPRNLSFSASG 345
             ||..:         |..|.....::.:|..|..:.....|......:.|.....|.....:.:.
  Fly   490 -TPLIITVLSAEEETTVGQTEALPTDESTTTTSTTTTTEKPSTTTSTSTTTTTPVPSTSKRTTTN 553

Human   346 TQLSLRWEPPADTGGRQDVRYSVRCSQCQGTAQDGGPCQPCGVGVHFSPGARGLTTPAVHVNGLE 410
            ..||....|...|..                                       |||:.
  Fly   554 APLSTTNTPTTTTSS---------------------------------------TTPST------ 573

Human   411 PYANYTFNVEAQNGVSGLGSSGHASTSVSISMGHAESLSGLSLRLVKKEPRQLELTWAGSRPRSP 475
                              .::...||:|..:...|.|       :::|:...:         |:.
  Fly   574 ------------------TTTSSTSTTVIPTTTKANS-------IIEKDKELI---------RAL 604

Human   476 GANLTYELHVLNQDEERYQMV---------LEPRVLLTELQPDTT-----YIVRVRMLTPL---- 522
            |..||.|:::   |:....:|         :.||   |:.||..|     .|:|..:||.:
  Fly   605 GPALTREINI---DDANNLIVFCNNTSDCGVTPR---TQTQPSHTTDSSPKILRTTVLTSIRSTV 663

Human   523 GPGPFSPD---HEFRTSPP------------------------VSRGLTGGE------------- 547
            .|.|.|..   ....|:||                        :|..:...|
  Fly   664 HPRPTSTSTTTSTTTTAPPAVTAPSNEVYIGIVESSSSASPADISSTVIANERDLNMEMRRMNLV 728

Human   548 -IVAVIFGLLLGAALLLGIL---VFRSRRAQRQ-------------------RQQRQRDRATDVD 589
             :|.|..|::..||::|.::   |.|.|..|.:                   .:.:..|...:||
  Fly   729 TLVLVAVGVIPLAAIILYLVRNFVIRRRAKQSEVFDVCITDQQPISPVKKVDSKYQVDDDEDEVD 793

Human   590 REDKLWLKPYVDLQAY---------------------------EDPAQGALDFTRELDPAWLMVD 627
            .:....::.:.:.|.:                           :|....|.:| .:.:.:.:.:.
  Fly   794 HQHHQHMQHHQNHQNHQNHQHHQAMPMSQASQRDANHNRYGNNDDKTSLASEF-HDFERSNIRLK 857

Human   628 TVIGEGEFGEVYR----------GTLRLPSQDCKTVAIKT---------LKDTSPGGQWWNFLRE 673
            :::|||.||:|::          |..|:       ||:||         |||            |
  Fly   858 SLLGEGNFGQVWKAEADDLSGHFGATRI-------VAVKTIRACSAQVSLKD------------E 903

Human   674 ATIMGQF-SHPHILHLEGVVTKRKPIMIITEFMENGALDAFLRERED----------------QL 721
            |.||.:. ||.:::.|.|...:.:|.|:|.|:...|.|.:.||....                .|
  Fly   904 ANIMRKLGSHQNVVTLLGACVESEPHMLIMEYAMRGRLLSLLRAARSATNILPASVPGGRSLAPL 968

Human   722 VPGQLVAMLQGIASGMNYLSNHNYVHRDLAARNILVNQNLCCKVSDFGLTRLLD----------- 775
            .|..|......||.||.|::....|||||||||:|::.|..||:.|||::..||
  Fly   969 SPRTLAGFALDIACGMEYIAGRRIVHRDLAARNVLLDHNGMCKICDFGMSIDLDAERMRKEQEKN 1033

Human   776 ---------------DFDGTY------------ETQG--------------------GK---IPI 790
                           ||...|            :.||                    ||   :||
  Fly  1034 AANDLMRHNAHKFKFDFGSRYILQHWQHTFGQGQGQGHCSKDQPHGEKKSHHGHDTIGKRHALPI 1098

Human   791 RWTAPEAIAHRIFTTASDVWSFGIVMWEVLSFGDKPYGEMSNQEVMKSIEDGYRLPPPVDCPAPL 855
            ||.|||::.:.:|||.:|:|:||||:||:.:.|..||.:::.:||::.:..|.|...|.:.....
  Fly  1099 RWMAPESLQYHMFTTETDIWAFGIVLWEIATLGSTPYSQLTGREVIRRVPQGLRPDLPKESRHEF 1163

Human   856 YELMKNCWAYDRARRPHFQKLQAHLE 881
            |.||..||..:...||.|  .|:.||
  Fly  1164 YNLMSRCWHKEPHMRPSF--AQSRLE 1187

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
EPHA1	NP_005223.4	EphR_LBD_A1	27..203	CDD:198447	48/297 (16%)
		fn3	334..431	CDD:394996	8/96 (8%)
		FN3	453..535	CDD:238020	21/102 (21%)
		EphA2_TM	<586..621	CDD:464211	6/61 (10%)
		Protein Kinases, catalytic domain	619..884	CDD:473864	101/360 (28%)
		SAM_EPH-A1	912..974	CDD:188941
		PDZ-binding. /evidence=ECO:0000255	974..976
Tie	NP_523928.1	TyrKc	854..1183	CDD:197581	98/349 (28%)