Protein Alignment Sema4b and Sema1a

DIOPT Version :9

• Sequence 1: NP_001347064.1
• Gene: Sema4b / 20352
• MGIID: 107559
• Length: 823
• Species: Mus musculus
• Sequence 2: NP_001260265.1
• Gene: Sema1a / 34192
• FlyBaseID: FBgn0011259
• Length: 1131
• Species: Drosophila melanogaster
• Alignment Length: 1022
• Identity: 258/1022 - (25%)
• Similarity: 388/1022 - (37%)
• Gaps: 331/1022 - (32%)

Mouse    14 LLLLLLLLLLRTTTTRALGPRISVPLGSEERLIRKFEAENISNYTAL----LLSQDGKTLYVGAR 74
            |||.:|::..::.....:.|::.|.||.|:  :.||    :.|.:.:    |:::||.:|.:|||
  Fly    54 LLLTVLVIGNQSAWQENIRPKLYVELGPED--VLKF----VGNESVVDHFKLVTKDGNSLLIGAR 112

Mouse    75 EALFALNSNLSFLPGGEYQELLWSADADRKQQCSFKGKDPKRDCQNYIKILLPLNSSHLLTCGTA 139
            ..:|    |||.....|.|.|:|::..|..:.|..|||| :..|||||:|::..:...|..|||.
  Fly   113 NTVF----NLSIHDLVEQQRLVWTSPEDDTKMCLVKGKD-EEACQNYIRIMVVPSPGRLFVCGTN 172

Mouse   140 AFSPLC-AY-IHIASFTLAQDEAGNVILEDGKGRCPFDPNFKSTALVVDGELYTGTVSSFQGNDP 202
            :|.|:| .| |..:::||...:.|..:       ||:||...||:::.|.|||:|||:.|.|:||
  Fly   173 SFRPMCNTYIISDSNYTLEATKNGQAV-------CPYDPRHNSTSVLADNELYSGTVADFSGSDP 230

Mouse   203 AISRSQSSRPTKTE--SSLNWLQDPAFVASAYVPESLGSPIGDDDKIYFFFSETGQEFEFFENTI 265
            .|.|    .|.:||  .||: |..|.||:|          ....|.:||||.||..||......|
  Fly   231 IIYR----EPLQTEQYDSLS-LNAPNFVSS----------FTQGDFVYFFFRETAVEFINCGKAI 280

Mouse   266 VSRVARVCKGDEGGERVLQQRWTSFLKAQLLCSRPDD-GFPFNVLQDVFTLNPNPQ-DWRKTLFY 328
            .||||||||.|:||....:.|||||||::|.||.|.| .|.||.:|....|..... .....|.|
  Fly   281 YSRVARVCKWDKGGPHRFRNRWTSFLKSRLNCSIPGDYPFYFNEIQSASNLVEGQYGSMSSKLIY 345

Mouse   329 GVFTSQWHRGTTEGSAICVFTMNDVQKAFDGLYKKVNRETQQWY-TETHQVPTPRPGACITNSAR 392
            |||.:.  ..:..|||:|.|.:.|:...|:|.:|:.......|. ....:||.||||:|
  Fly   346 GVFNTP--SNSIPGSAVCAFALQDIADTFEGQFKEQTGINSNWLPVNNAKVPDPRPGSC------ 402

Mouse   393 ERKINSSLQLPDRVLNFLKDHFLMDGQVR---SRLLLLQPRA--RYQRVAVH---RVPGLHSTYD 449
               .|.|..|||..|||:|.|.|||..|.   |:.:|::...  |:.::||.   :.|| ..|||
  Fly   403 ---HNDSRALPDPTLNFIKTHSLMDENVPAFFSQPILVRTSTIYRFTQIAVDAQIKTPG-GKTYD 463

Mouse   450 VLFLGTGDGRLHKAVTLSSRVH-------IIEELQIFPQGQPVQNLLL------------DSHGG 495
            |:|:||..|::.|:|...|...       :|||:.:..:.:|::||.:            ....|
  Fly   464 VIFVGTDHGKIIKSVNAESADSADKVTSVVIEEIDVLTKSEPIRNLEIVRTMQYDQPKDGSYDDG 528

Mouse   496 LLYASSHSGVVQVPVANC--SLYPTCGDCLLARDPYCAW-------------------------- 532
            .|...:.|.||.:.:..|  ....:|.:|:..:||||||
  Fly   529 KLIIVTDSQVVAIQLHRCHNDKITSCSECVALQDPYCAWDKIAGKCRSHGAPRWLEENYFYQNVA 593

Mouse   533 TG--SACRLASLYQPD---------------LASRPWTQD----------IEGASVKELCKNSSY 570
            ||  :||....:...|               |.||..::|          .||..:.....|:.|
  Fly   594 TGQHAACPSGKINSKDANAGEQKGFRNDMDLLDSRRQSKDQEIIDNIDKNFEGPQISADIINAQY 658

Mouse   571 KARFLVP--------------------GKPCK------------------------------QVQ 585
            ....||.                    |:.|.                              ::|
  Fly   659 TVETLVMAVLAGSIFSLLVGFFTGYFCGRRCHKDEDDNLPYPDTEYEYFEQRQNVNSFPSSCRIQ 723

Mouse   586 IQPNTV---------------------------NTLACPLLSNLATRLWVHNGAPVNASASCRVL 623
            .:|..:                           |||..|.:..:      |.|
  Fly   724 QEPKLLPQVEEVTYAEPVLLPQPPPPNKMHSPKNTLRKPPMHQM------HQG------------ 770

Mouse   624 PTGDLLLVGSQQGLGVFQCWSIEEGFQQLVA---------------------------------- 654
            |..:.|......|....|.:...:.|..|.:
  Fly   771 PNSETLFQFQPDGYNTQQSYRGRDNFGTLRSHQVMGDNYRRGDGFSTTRSVKKAVNNTNTRNRSL 835

Mouse   655 -----------------SYCPEVMEEGVMDQKNQRDGTPVIINTSRVSAPAGGRASWGADKSYWN 702
                             |..|:..::......:....:||:.|:|  |:||...:|....:|..|
  Fly   836 GRARRQPPRHGIVTQHRSNSPQQQQQQSQQPHSSSGSSPVMSNSS--SSPAPPSSSPSPQESPKN 898

Mouse   703 EFLVMCTLFVFAMVLLFLFFLYR-----HRDGMKLFLKQGECASVHPKTRPIVLPPETRPLNGVG 762
                 |:            ::||     :...|.|...|...|..||::.|..|||.       |
  Fly   899 -----CS------------YIYRDXLICNTKSMPLIQAQSTHAQPHPQSHPHPLPPP-------G 939

Mouse   763 PPSTPLDHRGYQALSDSSPGPRVFTESEKRPLSIQDSFVEVSPVCPR 809
            |.:.|...|....:..     |.:.:|           :.|:||.|:
  Fly   940 PTTPPAQPRARSPMIG-----RTYAKS-----------MPVTPVQPQ 970

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Sema4b	NP_001347064.1	Sema	48..511	CDD:417757	174/500 (35%)
		PSI	512..558	CDD:396154	18/100 (18%)
		Ig_Sema4B_like	579..663	CDD:409456	17/191 (9%)
		Ig strand B	592..596	CDD:409456	3/3 (100%)
		Ig strand C	604..608	CDD:409456	0/3 (0%)
		Ig strand E	626..629	CDD:409456	0/2 (0%)
		Ig strand F	639..644	CDD:409456	1/4 (25%)
		Ig strand G	656..659	CDD:409456	0/2 (0%)
Sema1a	NP_001260265.1	Sema_1A	91..546	CDD:200498	171/493 (35%)
		PSI	545..>579	CDD:214655	9/33 (27%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3611
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR11036
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	4	3.910