Protein Alignment Scn8a and NaCP60E

DIOPT Version :10

• Sequence 1: XP_017172001.1
• Gene: Scn8a / 20273
• MGIID: 103169
• Length: 1988
• Species: Mus musculus
• Sequence 2: NP_001261172.1
• Gene: NaCP60E / 37981
• FlyBaseID: FBgn0085434
• Length: 2896
• Species: Drosophila melanogaster
• Alignment Length: 2676
• Identity: 821/2676 - (30%)
• Similarity: 1170/2676 - (43%)
• Gaps: 899/2676 - (33%)

Mouse    53 PKPNSDLEAGKSLPFIYGDIPQGLVAVPLEDFDPYYLTQKTFVVLN---RGKTLFRFSATPALYI 114
            |:..:.:|.|..||..:...|:.:...|||:.|. ::.::||.|::   |...:.||:.|.:|::
  Fly    45 PRTKASVEDGDVLPRKFEPFPEHMYGKPLEEIDT-FIYEETFCVVSKRFRKNYIHRFTGTKSLFL 108

Mouse   115 LSPFNLIRRIAIKILIHSVFSMIIMCTILTNCVFMTFSNPPEWSKNVEYTFTGIYTFESLVKIIA 179
            ..|::..||:.:.|..:..|...:|.|||.||:|:..:   |..:..||.|..||:.|.::||||
  Fly   109 FYPWSPARRVCVYIATNQFFDYCVMATILFNCIFLAMT---ETVEEAEYIFLAIYSIEMVIKIIA 170

Mouse   180 RGFCIDGFTFLRDPWNWLDFSVIMMAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGAL 244
            :||.::.:|:||:|||||||.||...|.|..:::||::.||||||||||||:|::||||||:.||
  Fly   171 KGFLLNKYTYLRNPWNWLDFVVITSGYATIGMEVGNLAGLRTFRVLRALKTVSIMPGLKTIINAL 235

Mouse   245 IQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCV-----VWPINFNESYLENGTRGFDWEE 304
            :.|.::|::||.||:|||.||||..||::||.||||||     .|.   |.|:.       ||:.
  Fly   236 LHSFRQLAEVMTLTIFCLMVFALFALQVYMGELRNKCVRQVPTDWT---NVSHT-------DWQI 290

Mouse   305 YINNKTNFYMVPGMLEPLLCGNSSDAGQCPEGFQCMKAGRNPNYGYTSFDTFSWAFLALFRLMTQ 369
            ::|:..|:......| |:||||.:.|..||..:.|:..|.|||:|||:||.|.|:.|..|:|:|.
  Fly   291 WVNDTDNWLYDEDEL-PVLCGNLTGARHCPFEYVCLCVGENPNHGYTNFDNFMWSMLTTFQLITL 354

Mouse   370 DYWENLYQLTLRAAGKTYMIFFVLVIFVGSFYLVNLILAVVAMAYEEQNQATLEEAE------QK 428
            |||||:|.:.|...|...:.||.:|:|.|||||:||:|||||::|||:.:.|.||.:      :.
  Fly   355 DYWENVYNMVLATCGPMSVSFFTVVVFFGSFYLINLMLAVVALSYEEEAEITNEERKKDLLDHRD 419

Mouse   429 EAEF---------KAMLEQLKKQQEEAQAAAMA-----------TSAGTVSEDAIEEEGEDGVGS 473
            ::.|         |.:.:..||:.:..:...:|           |..|       :|.|.:|.|:
  Fly   420 DSTFSFDPSVLNVKKLNKNNKKKIDSRKGVLLASYSKKKTRRKKTKGG-------KEGGTNGNGN 477

Mouse   474 PRSSSELSKLSS---------KSAKERRNRRKKRKQKELSEGEEKGDPEKVFKSESEDGMRRKAF 529
            ..:..:....|:         .||.||.:....:.||:..:.|::   .|:.||.| .|......
  Fly   478 GSNGDDNKSHSATPSPGPSPRHSATERPSALTMQAQKQYQQMEQQ---HKLAKSGS-GGSNNPMA 538

Mouse   530 RLPDNRIGRKFSIMNQSLLSIPGSPFLSRHNSKSSIFSFRGPGRFRDPGSENEFADDEHSTVEES 594
            ..|..||.     ...|.:.:.....|...:.|..:.:..|.......|...|.:.|:...|::.
  Fly   539 PTPKGRIS-----FQDSGMGVKNPNMLYPSDYKGQLIANSGQPSSNSSGVNRESSQDDSGVVDDH 598

Mouse   595 E--------GRRDSLFIPIRARERRSSYSGYSGYSQCSRSSRIFPSLRRSVKRNSTVDCNGVVSL 651
            |        |...::.:.:..||.|                              .:.|||.::.
  Fly   599 EEQDTTNDMGHVSTVELALSPREVR------------------------------LIKCNGNIAR 633

Mouse   652 IGPGSHIGRLLPEVKIDKAATDSATTEVEIKKKGPGSLLVSMEQLASYGRKDRINSIMSVVTNTL 716
            | ...::..|..|.          ::||                                   .:
  Fly   634 I-KNHNVYALHQEF----------SSEV-----------------------------------VV 652

Mouse   717 VEELEESQRKCPPCWYKFANTFLIWECHPY--WIKLKEIVNLIVMDPFVDLAITICIVLNTLFMA 779
            :::|.:  |.|..|.:        | |..|  |::.:..:..:|.||..:||||:||||||.|:|
  Fly   653 IDDLPD--RNCDRCVH--------W-CTDYESWLQFQNCLYKVVRDPLFELAITLCIVLNTAFLA 706

Mouse   780 MEHHPMTPQFEHVLAVGNLVFTGIFTAEMFLKLIAMDPYYYFQEGWNIFDGFIVSLSLMELGLAD 844
            ||||.|:..|.:.|.|||.|||.|||.|..:||:|:.. .:|..||||||..||:.||:::....
  Fly   707 MEHHGMSESFRNALDVGNKVFTSIFTFECIVKLMALSK-DFFLCGWNIFDLLIVTASLLDIIFEL 770

Mouse   845 VEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSY 909
            |:||||||..|||||.|||:||.|:.:|:.||.:::||||||||:|.|:::||||:|||||.|.|
  Fly   771 VDGLSVLRGLRLLRVLKLAQSWTTMKVLLSIIISTIGALGNLTLILVIVIYIFAVIGMQLFSKDY 835

Mouse   910 KECVCKISQECKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCM---EVAGQAMCLIVFMMVMVI 971
            ..  .|...: .:|||:.|||||||:::||:|||||||.:||||   |..|.:.|..:|:..:|:
  Fly   836 TP--EKFDPD-PVPRWNFNDFFHSFMMIFRILCGEWIEPLWDCMRAEEEQGASTCFAIFLPTLVM 897

Mouse   972 GNLVVLNLFLALLLSSFSADNLAATDDD-GEMNNLQISVIRI----------------------- 1012
            ||.:||||||||||:||:::.|.:..:: ||.:.|..|:.|:
  Fly   898 GNFMVLNLFLALLLNSFNSEELKSKKEEVGEESKLARSIERVRDLIRKKRQERKDRKERKFAEKF 962

Mouse  1013 ---------------------------KKGVAWAKVKVHAF------------------------ 1026
                                       |.||. |:.|.|..
  Fly   963 QQIVLDAQQAHAQTLSHQAAVGLERGDKPGVL-AETKFHRLSYQESMNRPVSGSDFGFQIPLHDG 1026

Mouse  1027 ---------------------MQAHFKQREADEVKPLDELYEKKANCIANHTGVDIHRNGDFQK- 1069
                                 :|||.....:|.:.|   .||   :.:...||      |.|..
  Fly  1027 LHTIVDGLEYDDTGDLPEQIQLQAHPLPPTSDSMPP---TYE---SAMMATTG------GSFSSV 1079

Mouse  1070 NGNGT---------------------------------------------------------TSG 1077
            |||||                                                         .||
  Fly  1080 NGNGTCQNLTPFVQAERRLQHQISSGVSTQQYDSREEATYTESIELLGQYNSTDTDPYANDQRSG 1144

Mouse  1078 IGS----------SVEKYIIDEDHMSFIN-NPNLTVRVP-------------------------- 1105
            .||          |..:|..:|...:|:. ..:|..|.|
  Fly  1145 CGSFNRGDSLQDNSSRRYGSEEHDEAFLKYQKSLLTRSPSYRKSLDRLSQSSGQSQRSLLKSEEA 1209

Mouse  1106 ----------------------------------------------------------------- 1105
                                                                             
  Fly  1210 EMRRHSSGQSLNSMSIEQDELLSQQGNLREELLNCDQKELFQFLQEEEELQKGTKLRRISNVMRS 1274

Mouse  1106 -------------IAVGESDFENL------NTEDV-----------SSESDPEGSKD-------- 1132
                         ..|..|:|:|:      ..|::           |:.|:|..:.|
  Fly  1275 RRPSSQMGQPENETMVEHSEFDNIIQSFEKELEEIKRSTTSLERKLSNLSEPSPAADEATKAIME 1339

Mouse  1133 -----------------------------------------------------------KLDD-- 1136
                                                                       |:|:
  Fly  1340 HIAIITGASERSAADEVVLPLNPYDSYDLSSVPRRSQSVSAAAQRQSVKLKRRSLEKQRKIDEDF 1404

Mouse  1137 ---------------------------TSSSEG----------------------STIDIKPEVE 1152
                                       ||:|:.                      ..:.:...||
  Fly  1405 SISNEIRKICDQIHAPFVAMEAMAVAATSASQAQPNQSPFLRRKVDPFTVQFDRFKRLSLIERVE 1469

Mouse  1153 EVP-------------------------------------------------------------- 1155
            |||
  Fly  1470 EVPEEEKPISTLRIESEKMPRKFLHGPDQLRLDSLSLKSTNSYENLLIQKQKLGMATPPAVPATP 1534

Mouse  1156 ----------------------------------------------------------------- 1155
                                                                             
  Fly  1535 PTSLKSSIEPPTLAQISSLKTTPPLAALTEHQQHFHATSIQAAPTPAHTHAHSQAHAHSMAGQRR 1599

Mouse  1156 -VEQPEEYLD------------------------------------------------------- 1164
             :|.|:..||
  Fly  1600 RMEHPQSTLDKAASFQSARTESHSSGAADASSALALAMAQKTEQSQSTAPDATQKPSAFTRLTEK 1664

Mouse  1165 -----------------------------------------PDACFTEGCVQR--FKC-----CQ 1181
                                                     ||.||.:.|...  |:|     |
  Fly  1665 PWHCLVSYVDDLTVGGRRNSQGAYNDPMTFPSYGATKAAKVPDDCFPQKCYDHFYFRCPWFMSC- 1728

Mouse  1182 VNIEEGLGKSWWILRKTCFLIVEHNWFETFIIFMILLSSGALAFEDIYIEQRKTIRTILEYADKV 1246
              ::....|.|..:|.....:|:...||.|::.:|..||..|.||||.:::.||::.:|.:.:..
  Fly  1729 --MDTQSAKHWTRVRTAVLTVVDTPAFEWFVLVLIFASSITLCFEDINLDKNKTLKRVLYWINFS 1791

Mouse  1247 FTYIFILEMLLKWTAYGFVKFFTNAWCWLDFLIVAVSLVSLIANALGYSELGAIKSLRTLRALRP 1311
            |..||::||:|||.|.||.|:||:.|..|||:||.||:.||:...  ...|..::||||||||||
  Fly  1792 FCLIFVVEMILKWLALGFSKYFTSFWTILDFIIVFVSVFSLLIEE--NENLKVLRSLRTLRALRP 1854

Mouse  1312 LRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYHYCFNETSEIRFEID 1376
            |||:||::|||:|||||:.|||||.|||||||:||||||||||..|.||:..|.||..|: ..|.
  Fly  1855 LRAISRWQGMRIVVNALMYAIPSIFNVLLVCLVFWLIFSIMGVQFFGGKFFKCVNEMGEL-LPIT 1918

Mouse  1377 EVNNKTDCEKLMEGNNTEIRWKNVKINFDNVGAGYLALLQVATFKGWMDIMYAAVDSRKPDEQPD 1441
            |||:|.||   :|.|.|   |.|.||.||:||.|||||||||||:|||::|..|||:|..|.||.
  Fly  1919 EVNDKWDC---IEQNYT---WINSKITFDHVGMGYLALLQVATFEGWMEVMADAVDARGVDLQPQ 1977

Mouse  1442 YEGNIYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGG--QDIFMTEEQKKYYNAMKKLG 1504
            .|.|:|.||||||||:.||||||||||||||||||..|||:.|  .::|:||.||.||.||||||
  Fly  1978 REANLYAYIYFVIFIVCGSFFTLNLFIGVIIDNFNMLKKKYEGGVLEMFLTESQKHYYTAMKKLG 2042

Mouse  1505 SKKPQKPIPRPLNKIQGIVFDFVTQQAFDIVIMMLICLNMVTMMVETDTQSKQMENILYWINLVF 1569
            .|||||.|.||:|....:.:|....:.|:|.|.:||.|||:||.:|...|...:..||...|..|
  Fly  2043 RKKPQKVIKRPINHFLAMFYDLSNSRRFEIAIFVLIFLNMLTMGIEHYDQPHAVFFILEVSNAFF 2107

Mouse  1570 VIFFTCECVLKMFALRHYYFTIGWNIFDFVVVILSIVGMFLADIIEKYFVSPTLFRVIRLARIGR 1634
            ...|..|.::|:..||::|||:.||:|||::|:.||.|:.:.||:....:||||.||:|:.||||
  Fly  2108 TTVFGLEAIVKIVGLRYHYFTVPWNVFDFLLVLASIFGILMEDIMIDLPISPTLLRVVRVFRIGR 2172

Mouse  1635 ILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIFSIFGMSNFAYVKHEAGIDDMFNFETF 1699
            ||||||.|||||.|||||::|||||||||.||.|:.||::|.|||.|..||.:..:|||.||:||
  Fly  2173 ILRLIKAAKGIRKLLFALVVSLPALFNIGALLGLITFIYAILGMSLFGNVKLQGALDDMVNFQTF 2237

Mouse  1700 GNSMICLFQITTSAGWDGLLLPILNRPPDCSLDKEHPGSGFKGDCGNPSVGIFFFVSYIIISFLI 1764
            |.||..||::.|||||:.:|..::.:||||  |....|. ..|:||:|.:.|.:|.|:||||::|
  Fly  2238 GRSMQLLFRLMTSAGWNDVLESLMIQPPDC--DPFIHGH-TNGNCGHPLLAITYFTSFIIISYMI 2299

Mouse  1765 VVNMYIAIILENFSVATEESADPLSEDDFETFYEIWEKFDPDATQFIEYCKLADFADALEHPLRV 1829
            |:|||||||||||:.|.:|....:.|||.|.||..|.|:||.|||||.:.:|:||..:|:.||.:
  Fly  2300 VINMYIAIILENFNQAHQEEEIGIVEDDLEMFYIRWSKYDPHATQFIHFSQLSDFIASLDPPLGI 2364

Mouse  1830 PKPNTIELIAMDLPMVSGDRIHCLDILFAFTKRVLGDSGELD---ILRQQMEERFVASNPSKVSY 1891
            .|||.:.|::.:||:..|::|||||||.|..|.|||...|.|   .|::||:.:|....|::...
  Fly  2365 SKPNNVALVSFNLPISKGNKIHCLDILHALVKHVLGHVEETDNFKQLQEQMDVKFKKQFPTRKEL 2429

Mouse  1892 EPITTTLRRKQEEVSAVVLQRAYRGHL-------------------------------------- 1918
            |.:::|...|::|.:|..:|..::.:|
  Fly  2430 EIVSSTRIWKRQEKAAKTIQTGWKEYLRRKREKERSNSGDSATQTSSPGGWQSKLSALNFFHLQV 2494

Mouse  1919 ARRGFIC-RKITSNKLENGGTHREKKESTPSTASLP-----SYDSVTKPDKEKQQRAEEGRR--- 1974
            :|||..| .:.:|.|........:..|......:||     ..|.|.|  ..|||..|.|:|
  Fly  2495 SRRGTACSSRASSRKSSRASDASDLSELAGPWLNLPLMLVSGADEVVK--DIKQQNDELGKRGSI 2557

Mouse  1975 ----ERAKRQK 1981
                .||.|::
  Fly  2558 FVEAPRASRRR 2568

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Scn8a	XP_017172001.1	Ion_trans	131..421	CDD:459842	140/294 (48%)
		Na_trans_cytopl	546..710	CDD:463401	21/171 (12%)
		Ion_trans	760..973	CDD:459842	116/215 (54%)
		Na_trans_assoc	999..1200	CDD:461936	72/835 (9%)
		Ion_trans	1205..1481	CDD:459842	161/275 (59%)
		Na_channel_gate	1473..1525	CDD:240441	30/53 (57%)
		Ion_trans	1530..1784	CDD:459842	132/253 (52%)
		IQ	1902..1923	CDD:197470	6/58 (10%)
NaCP60E	NP_001261172.1	Ion_trans	125..371	CDD:459842	121/259 (47%)
		Ion_trans	687..902	CDD:459842	118/218 (54%)
		Ion_trans	1751..2019	CDD:459842	163/276 (59%)
		Na_channel_gate	2009..2063	CDD:240441	30/53 (57%)
		Ion_trans	2067..2320	CDD:459842	133/255 (52%)
		GPHH	2332..2378	CDD:465306	20/45 (44%)