Protein Alignment EIF4G1 and NAT1

DIOPT Version :10

• Sequence 1: NP_001181875.2
• Gene: EIF4G1 / 1981
• HGNCID: 3296
• Length: 1606
• Species: Homo sapiens
• Sequence 2: NP_001033943.2
• Gene: NAT1 / 46020
• FlyBaseID: FBgn0010488
• Length: 1488
• Species: Drosophila melanogaster
• Alignment Length: 1178
• Identity: 276/1178 - (23%)
• Similarity: 450/1178 - (38%)
• Gaps: 370/1178 - (31%)

Human   674 TPSFANLGRTTLSTRGPPRGGPGGELPRGPA----GLGPRRSQQGPRKEPRKIIATVLMTEDIKL 734
            ||:..| |..:....|.........|..|.|    |..|...||..|.:..|......:|
  Fly   436 TPNLRN-GSASAQISGSTSNSSNISLNSGSASAAGGGTPHSQQQQERSQAGKASTPPPIT----- 494

Human   735 NKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEE 799
                ..|.|.|.|......:.|      |...:|||||.|||||||:.||:|..::.:|.:::..
  Fly   495 ----GRWIPPSLRPQHGLTQSE------KNDAVFRRVRGILNKLTPEKFQELSDELLKLDLNSIA 549

Human   800 RLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEK-PTVTVNFRKLLLNRCQKEF----E 859
            .|||||.|||:||:.||.:|..||.:|:.| :.:.|:.:| |:.:..|.:||:..|:.:|    :
  Fly   550 ILKGVILLIFDKALDEPKYSSMYAQLCKRL-SEEAPSFDKEPSNSSTFLRLLIAVCRDKFNNRLK 613

Human   860 KDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMH 924
            :|::|:.  ...:.|.||              ||.|.:|::|.|||:||||||.||.||::.::|
  Fly   614 RDENDNR--PPPENEADE--------------EERRHLAKQRMLGNVKFIGELNKLDMLSKNVLH 662

Human   925 DCVVKLLKNHDE---------ESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSS 980
            .|:::|.....:         |.:|||.:||.|.||:||.|:.|..|:|||.::|:..|..:...
  Fly   663 QCIMELFDKKKKRTAGTQEMCEDMECLAQLLKTCGKNLDSEQGKELMNQYFEKLERRSKSSEYPP 727

Human   981 RIRFMLQDVLDLRGSNWVPRR--GDQGPKTIDQIH----------------------KEAEMEEH 1021
            ||||||:||::||.:|||||:  ..:||..|.||.                      ::::...:
  Fly   728 RIRFMLKDVIELRQNNWVPRKLGTTEGPVPIKQIRSDDDSIIRTPFPNRNRDMRNNDRDSDSWMN 792

Human  1022 REHIKVQ-----------------------------------QLMAKGS---DKRRGGPPGPPIS 1048
            |.|:.:|                                   |...:|.   |:.:||..|....
  Fly   793 RFHLNIQPGGYNDMFSGLSVTGASPIVSPFATSNRDRDRDNRQYNNRGDRNRDRDQGGSGGANYG 857

Human  1049 RGLPLVDDGGWNTVPISKGSRPIDTSR-------------------------------------- 1075
            ......:..|..:...|..:|..|.||
  Fly   858 NRYNKHNQNGGGSGGGSSNNRDRDDSRRNNDRDRDRNDGQYGGNQLLGSKELAPRFKRNLITTNQ 922

Human  1076 -----------------------------LTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAK 1111
                                         |...|.|.|..||||            |||.|..:.
  Fly   923 DAVENLQMRPAANSLLFRAASQNQKFPATLPMSTPPNSSGSNNQ------------GSSQGQLSN 975

Human  1112 --------PSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKA------- 1161
                    |:.......||:::.           ..|..|||.::   .:.:|||.||
  Fly   976 SHYPMLGTPTSHLINPTRPSSTP-----------SAEYADNRTLL---GVQQERGLKATSSSPNF 1026

Human  1162 GDRGDRLERSERGGDRGDR----------LDRARTPATKRSFSKEVEERSRERPSQPEGLRKAAS 1216
            ....::|:...:.|.:|..          ::|..|||.....:|  :::..:.|::.|..:||:.
  Fly  1027 SSGAEKLDLDGQAGRKGSHDGKDQGKNGSVERPSTPAGGIGSAK--QQKKDKGPNKEELSKKASQ 1089

Human  1217 LTEDR---DRGRDA---------------------------VKREAALPPVSP----------LK 1241
            ..:||   |...||                           ||.:||:|..:|          |:
  Fly  1090 YFKDRFYYDEEEDAEVTEIEIKDIDETKTEEADAAPVEVSEVKEDAAIPVTTPDRFTKLVDGFLE 1154

Human  1242 AALSEEELEKKSKAIIEEYL-HLND--MKEAVQCVQEL-----ASPSLLFIFVRHGVESTLERSA 1298
            ..|.|:.|:.....::.|.| .:||  ::.||:.:|.|     ..|:::....:..|....||.|
  Fly  1155 LKLPEKALKDVCINLLMEVLDRVNDVFLERAVRLLQTLRKQSNIKPNIVVEIFKQVVNKMNEREA 1219

Human  1299 I---AREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGV 1360
            :   ....:..||.:.:|...|..      |.:|....::
  Fly  1220 LNPRIVSLVASLLAKTICEPALLK------LSDIANFTDN------------------------- 1253

Human  1361 PMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEG----QDIG 1421
                            |:...|.|.:|..|.|.:|...:...:|.:.:.....|||.    :.:.
  Fly  1254 ----------------GQHYPLFLLVLQQLVKVIGKDALEEKFRASKVDLMNSLPEADRNKERLA 1302

Human  1422 AFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLV 1486
            ..:.::|:.:            ..|..::..::.|.|:...:....:.||:||:..:.......:
  Fly  1303 GILEDRKLSF------------LYPLLKVQAEMLKQLQSDPNPNNFYKWIKANVDNKYYKDPGFI 1355

Human  1487 RALMTAVCYSAIIFETPL---------------RVDVAVLKARAKLLQKYLCDEQKELQALYALQ 1536
            :||||.| ...:..||.|               :.:..:|:..:::||.:|...:.:|.||||||
  Fly  1356 QALMTVV-VKYVTKETTLADNLDPKEHPEKSVTQKEQQLLENYSQMLQTFLGQNELQLMALYALQ 1419

Human  1537 ALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWE---SSKDPAEQQGKGVALKSVTAFFKWLRE 1598
            ........|..:|..:|..||:.::::|:||..|:   |.|.|    |||.||..|.|:..||:|
  Fly  1420 TFCYNENFPKGMLCRWFKYLYESEIIEEEAFVLWKEEISDKYP----GKGSALFQVNAWLTWLQE 1480

Human  1599 AEEESDHN 1606
            ||.|.|.:
  Fly  1481 AESEDDED 1488

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
EIF4G1	NP_001181875.2	PHA03247	<21..583	CDD:223021
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	173..229
		PABPC1-binding	179..207
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	246..326
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	339..607
		EIF4E-binding	614..625
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	674..720		13/49 (27%)
		eIF3/EIF4A-binding	689..1092		146/549 (27%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	738..764		5/25 (20%)
		MIF4G	768..993	CDD:397130	94/238 (39%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1031..1234		55/327 (17%)
		MA3	1249..1361	CDD:397128	20/122 (16%)
		W2_eIF4G1_like	1445..1577	CDD:211397	37/149 (25%)
		EIF4A-binding	1457..1606		50/166 (30%)
		Necessary but not sufficient for MKNK1-binding	1592..1606		7/13 (54%)
NAT1	NP_001033943.2	MIF4G	518..743	CDD:397130	96/241 (40%)
		RAM	<817..860	CDD:464643	6/42 (14%)
		MA3	1128..1241	CDD:214714	26/112 (23%)
		W2_eIF4G1_like	1314..1460	CDD:211397	36/146 (25%)