Protein Alignment EIF4G1 and eIF4G2

DIOPT Version :10

• Sequence 1: NP_001181875.2
• Gene: EIF4G1 / 1981
• HGNCID: 3296
• Length: 1606
• Species: Homo sapiens
• Sequence 2: NP_651188.1
• Gene: eIF4G2 / 42819
• FlyBaseID: FBgn0260634
• Length: 2072
• Species: Drosophila melanogaster
• Alignment Length: 2062
• Identity: 414/2062 - (20%)
• Similarity: 684/2062 - (33%)
• Gaps: 680/2062 - (32%)

Human     5 PQSTGPPPAP-----SPGLPQPA-------------FPPGQTAPVVFSTPQATQMNTPSQPRQGG 51
            |::...|..|     |.||..||             .|..||.|::...|..|   .|:....|.
  Fly   169 PRALSNPTLPPHFQQSAGLIAPAGGNAGGPYISATYIPINQTGPLMGMQPAVT---FPTNGAAGA 230

Human    52 FRSLQHFY------PSRAQPPSSAASRVQSAAPA----------RPGPAAHVYPAGSQVM----- 95
                 .||      |.:.|.|.:|..:....||.          :.||.|. ..||:.|:
  Fly   231 -----SFYAAAPPPPQQKQLPPTAQQQGPGGAPGAAQTPTLAYYQLGPNAQ-NSAGNNVVSGANT 289

Human    96 ----MIPSQISYPASQGAYYI------PGQGRSTYVVPTQQYPVQPGAPGFYPGAS-----PTEF 145
                |...|.|.|.:|...|.      ||..:..  .||...|:.....|...||:     |..|
  Fly   290 HRGNMRGGQRSTPTAQHTNYFTAYTMAPGNAQRP--APTNVAPMMNSRSGALNGAAAFHHLPHNF 352

Human   146 GTYAGAYYPAQGVQQ---FPTGV-APTPVLMNQPP----QIAP--KRERKTIRIRDPNQGGKDIT 200
            |..:.|..|..|:..   .|.|: ||.|    |||    ..||  :....|.....|:..|   .
  Fly   353 GIPSLALGPPAGMTTPILAPAGMQAPPP----QPPGGLHTFAPHLQSNSATTLPSVPSVTG---V 410

Human   201 EEIMSGARTASTPTPPQTGGGLEPQANGETPQVA------VIVRPDDR-------SQGAIIADRP 252
            ..:.||..|.|.|||..|   ..||....:.:|.      .|:.||..       .:...|....
  Fly   411 PAVASGFSTTSLPTPSVT---TAPQLGANSDKVCKRKYAIPIIHPDTMENVMDGWKRSVFIKKDS 472

Human   253 GLPGPEHSPSESQPSSPS-------PTPSPSPVLEPGSEPNLAVLSIPGDTMTTIQ--MSVEEST 308
            .....:|:..::.....|       ||     :::.||..:|...::....|.|::  .|:::|.
  Fly   473 NSTLTQHTDLDTALDDNSTLCLYQAPT-----LMDSGSGSSLLSGAMVDQQMQTVKPASSLKQSV 532

Human   309 PISR---------ETGEPYRLSPEP--------TPLAEPIL----EVEVTLS-------KPVPES 345
            .:.:         ..|:....||.|        :.:.:.||    ..|..||       ..|.|:
  Fly   533 SLEKVPEAVYCKDAVGQDVHPSPPPKSTDKDQISVIVKDILAHSGNAEDHLSFWQYSDVDNVTET 597

Human   346 EFSSSPLQAPTPLASHTVE----------------IHEPNGMVPSEDLEPEVESSPELAP----- 389
            ..|...:|:..|..:.|.:                .::.:...|:.......|::....|
  Fly   598 HNSLPLVQSDLPDTTDTCQQPVLFQEQAKKQRPKLANKQSTATPASIANDVAEAAASGCPGVSIL 662

Human   390 -------PPACPSESPVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSA 447
                   .||.|| :|.|.:..||.:...:...:..|..:.|.:....:...:.|:.:..|...|
  Fly   663 SRGQSMVTPAGPS-TPPPPSRVAQQKSTKSIVTTAVAASVKPTTGSPNRKSSIQAATSTTTSSGA 726

Human   448 TPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLEAAAAT 512
            :.:||.:.|..:.:.:.:.....:.|:....      |.....|||.::.|.|.::.....|..|
  Fly   727 SSSTASTPTHASSKLKQQPVNNLQHGKQIPG------KVSTPALPPTASRISAAVTLLAGTANIT 785

Human   513 QVAVSVPKRR---RKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPAGSNPGPESEGSG----- 569
            ....|..|:|   ::.:||.||          |:.:........|   |.|...:..|:|
  Fly   786 TTTNSSKKQRQAQKRARELEKK----------KQKSSITSTTSTQ---GQNNNGKGNGNGNAALE 837

Human   570 -------------------------VPPRPEEADETWDSKE-----------------------D 586
                                     ..|..::.....|.||                       |
  Fly   838 INTNSNNAATVTTTSMSTLTTTTTATAPTTKQETAAVDDKETAQPTTDTLSATSNNTKVSSILRD 902

Human   587 K-----------IHNAENIQPGEQKYE----------YKSDQWKPLNLEEKKRY--DREFLLGF- 627
            |           .||..|....|...|          .|.|....:..|:.:::  :.|.:..|
  Fly   903 KKTMLNPKEQLTSHNNNNNNNNEDGIEAKVESDPESDIKDDSMSNITGEKSEKHLANEEIVAKFL 967

Human   628 -----------------QFIFAS---MQKPEGLPHISDVVL-----------------DKANKTP 655
                             ||:.:|   .::.|.:|.||:.|.                 |..|.|.
  Fly   968 QKDSPDTSVVHAETQQLQFLNSSSSVCEEDEPVPVISEGVTKTEENAEAEIKFGDFHEDPRNDTG 1032

Human   656 L-----------------------RPLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPP-----R 692
            .                       |..|     .::|.|......:. .::..||:..|     .
  Fly  1033 FICSSTWSSSTISKAAEDDSSQSSRSAD-----NVDCAPKVVEQISE-EKSASSTKSSPVHKATG 1091

Human   693 GGPGG--------------ELPRGPAG----------LGPRRS---------------------- 711
            .|||.              .:|:.|..          |..||.
  Fly  1092 SGPGSPKTKELKSKSPSPIPVPQVPKNYRYSIETLRELAKRRDSRKPPMVPCQKGDCISQLFVSR 1156

Human   712 QQGPRKE-----------------------------PRK-------------------------- 721
            ||.|::.                             |.|
  Fly  1157 QQQPQQHHGQHIQQYQHMSFNESLDYVPGKRGRGHGPNKKHHDQHQQMGGSSGMGSGSGGGGGIS 1221

Human   722 --------IIATVL-MTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNK 777
                    ||...| :.|:|||::.|.||:|.:.|..:.....:||.|   .:.:.::||.||||
  Fly  1222 NSSQRHMDIIRVQLSLKEEIKLSECENAWQPGTLRRVSMAGGQDEDDD---VEGVLKKVRGILNK 1283

Human   778 LTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTV 842
            |||..|:.|:|:::.:.:|.|.::..|:.|||||.||||||:..||..|:.|.. ::....|..
  Fly  1284 LTPDNFEVLLKEMSSIKMDNEAKMTNVMLLIFEKTISEPNFAPTYARFCKVLFH-EIKAENKSL- 1346

Human   843 TVNFRKLLLNRCQKEFEKDKDDDEVFEKK----QKEMDEAATAEERGRLKEELEEARDIARRRSL 903
               |...|:.|.|.|||.:.:|.....||    .:.:::.:...::..|:.|:|:.....|||:.
  Fly  1347 ---FTSSLITRIQHEFESNVNDANAKSKKLQPTMERINQCSDPAKKAELRAEMEDLEYQFRRRAW 1408

Human   904 GNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFE-----KAKPRMD 963
            |.::||||||||:.||...:.:||..||::..||.||.:|:||||:|..|:..     :.:.|::
  Fly  1409 GTVRFIGELFKLQSLTNDRVLNCVESLLEHGCEEKLEYMCKLLTTVGHLLESSLPEHYQLRDRIE 1473

Human   964 QYFNQMEKIIKEK-----------KTSSRIRFMLQDVLDLRGSNW----VPRRGDQGPK------ 1007
            :.|.:::.||...           |.|||:|||:|||||||..||    ..:.|.||.:
  Fly  1474 KIFRRIQDIINRSRGTSHRQQVHIKISSRVRFMMQDVLDLRLRNWDQPATHQSGQQGGRGQRHKQ 1538

Human  1008 ---TIDQIH-----------KEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGG 1058
               :.||.|           :..:...|.:|.|..|         .|...|....:.:|
  Fly  1539 HDDSKDQSHSTGGGRGGGMNQHQQSSTHHQHQKHNQ---------HGHDGGNYFMQKMP------ 1588

Human  1059 WNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPA 1123
             |.......:..||.|:| :.:...:.|.:...........|  .:.|......:...:..|.|.
  Fly  1589 -NKQHHENQTLSIDPSKL-RFSNSSATDDSIAKLGNSSHYQW--RNVGNRPVSSTQVNASNAPPP 1649

Human  1124 TSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPA 1188
            ||.|.|.....:.:|.:        |.|:::......||.....:|.:       |..|..:...
  Fly  1650 TSLLKRPGQNYKFLPYQ--------QPSAVAGTANAPAGGNKSNVEVN-------DAFDGKQCQG 1699

Human  1189 TKRSFSKEVEERSRERPSQPEGLRKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEELEKKS 1253
            .   .:|.|||....|..|||                           |..:..:.|..:..|..
  Fly  1700 L---ITKLVEEALNTREWQPE---------------------------VLSIWRSHSGSQQSKTL 1734

Human  1254 KAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLS 1318
            ..::.:|||.:.:|.     |...:...:|.::       :::.|:.:
  Fly  1735 FHLLTDYLHKSTVKR-----QHRQACGNIFAYL-------MDKDAVEK----------------- 1770

Human  1319 TAQYYQGLYEILELA--------EDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRP 1375
                     ||.|.|        .|:.:|:|:.|.|:.|.:.||:..|.:.:.::::.  :.|..
  Fly  1771 ---------EIFEEAYSRFGDDFPDLLVDVPNGWGYVFEFLGPIIHSGHLDLKDVWQR--RWLDD 1824

Human  1376 LGKAASLLLEILGLLCKSMGPKKVGTLWR------EAGLSW------KEFLPE----------GQ 1418
            ...|...:...:....:..|......||.      ...|.|      :||:..          ||
  Fly  1825 FFFAERFVYAFVSYFVQEFGAAYARKLWHNDYKLDRGQLFWSDVRKYREFVQSHSFYFLDNGPGQ 1889

Human  1419 DIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSN 1483
            :.|    ..||        :||.....|.|.:.| :..||  ..|.....|:|..|::    :::
  Fly  1890 NQG----HAKV--------KAPTTPRSPVEHVER-IRHLL--AISCDMAIDYINTNVA----IND 1935

Human  1484 TLVRALMTAVCYS---AIIFET-----------PLRVDVAVLKARAKLLQKYLCDEQKEL----- 1529
            ..||.|...:|..   .::..|           ||:::....::....|.:...|.|:.|
  Fly  1936 KFVRQLTRFLCCDFALTVMTNTHNNIKSGGNSKPLQLNTESFRSCCTPLLRLCIDAQEALEIACI 2000

Human  1530 -QALYALQALVVTLEQPPNL-----LRMFFDALYDEDVVKEDAFYSW 1570
             :|:.|||...:| |...::     :...|..||:.:|:.:::|..|
  Fly  2001 DEAVDALQQHFMT-EYEDDMAAGETICSTFSVLYESEVIPKESFDKW 2046

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
EIF4G1	NP_001181875.2	PHA03247	<21..583	CDD:223021	140/731 (19%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	173..229		18/61 (30%)
		PABPC1-binding	179..207		6/29 (21%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	246..326		16/105 (15%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	339..607		60/379 (16%)
		EIF4E-binding	614..625		2/12 (17%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	674..720		14/125 (11%)
		eIF3/EIF4A-binding	689..1092		141/561 (25%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	738..764		8/25 (32%)
		MIF4G	768..993	CDD:397130	89/244 (36%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1031..1234		33/202 (16%)
		MA3	1249..1361	CDD:397128	21/119 (18%)
		W2_eIF4G1_like	1445..1577	CDD:211397	32/151 (21%)
		EIF4A-binding	1457..1606		29/139 (21%)
		Necessary but not sufficient for MKNK1-binding	1592..1606
eIF4G2	NP_651188.1	Herpes_BLLF1	<114..450	CDD:282904	74/301 (25%)
		MIF4G	1274..1514	CDD:397130	89/244 (36%)
		MA3	1697..1812	CDD:397128	31/182 (17%)