Protein Alignment Rb1 and Rbf

DIOPT Version :10

• Sequence 1: NP_033055.2
• Gene: Rb1 / 19645
• MGIID: 97874
• Length: 921
• Species: Mus musculus
• Sequence 2: NP_525036.2
• Gene: Rbf / 31027
• FlyBaseID: FBgn0015799
• Length: 845
• Species: Drosophila melanogaster
• Alignment Length: 984
• Identity: 221/984 - (22%)
• Similarity: 376/984 - (38%)
• Gaps: 258/984 - (26%)

Mouse    24 EDDPAQDSGPEELP-LARLEFEEIE--EPEFIALCQKLKVPDHVRERAWLTWEKVS---SVDGIL 82
            |.|| |:.|.|.:. |.....:.:|  ..|:..||:.|.:......:.:.|:.:||   |::|..
  Fly     3 EPDP-QELGAEVVSGLVATSDDRLEMINAEYTNLCRDLNMDRQTELQGYETYLEVSQRCSMEGTA 66

Mouse    83 EGYIQKKKELWGICIFIAAVDLDEMP-------------FTFTELQKSIETSVYKFFDLLKE--- 131
            ..        |..|....|......|             .:...|.:..:.|:|:|...:|:
  Fly    67 SH--------WMCCAIYTACRRTSTPTVTGQNAVVKGNCVSLNNLLRCCKMSIYEFKTKIKQWCD 123

Mouse   132 -IDTSTKVDNAMSRLLKKYNVLCALYSKLERTCELIYLTQP-----SSALSTEIN------SMLV 184
             .:...:..|.:..|.:|:::...|:.:.....::|:...|     |..:|...|      |.:.
  Fly   124 MANLPQEFVNEIEDLDRKFSITFMLHKRFRIIMDMIFSCPPNEKKHSKYISLHGNHAHGKCSYIK 188

Mouse   185 L-KISWITFLLAKGEVLQMEDDLVISFQLMLCVVDYFIKFSPPALLREPY------KTAAIPING 242
            | .|.|..||.||.:......|||.|:.||:|.:|....    .:|.|..      |...:|.|.
  Fly   189 LDDICWRLFLCAKNQKPSNTVDLVTSYNLMICCIDLIYN----NVLAEKRTDLINPKFEGLPSNW 249

Mouse   243 SPRTPRRGQNRSARIAKQLENDTRIIEVLCKEHECNIDEVKNVYFKNFIPFINS----------- 296
            :....|             .|...|:...|...|    |.|.:....|...::|
  Fly   250 TELDFR-------------HNPHCILSNFCDMTE----EAKAMKATTFRQIMSSFFQASTIYGNK 297

Mouse   297 ---LGIVSSNGLP-EVESLSKRYEEVYLKNKDLDARLFLDHDKTLQT--------DPIDSFETER 349
               ||::::.... .::||:..||:..|...:.|.|:...:|....|        .|:    |..
  Fly   298 DTMLGLLANENFERNLKSLNISYEQYVLSVGEFDERILSAYDAGEHTALNDQSLRPPV----TPL 358

Mouse   350 TPRKNNPDEEANVVTPHTPVRTVMNTIQQLMVI--LNSASD---QPSENLISYFNNCTVNPKENI 409
            |.:::.|.:.|.......|||...|.::||...  :...:|   |..|.:|:           .:
  Fly   359 TRKQDLPAQPAMAGDKFEPVRNATNNVKQLSAFGRITEPTDFVKQAGEEVIA-----------KL 412

Mouse   410 LKRVKDVGHIFKEKFANAVGQGCVDIGVQRYKLGVRLYYRVMESMLKSE--------EERLSIQN 466
            |..::::...|..|:.:...:       .|::|....::.:::.:|::|        .:||.:|.
  Fly   413 LSIIEEIEQKFLAKYPSTEAK-------SRFQLAKSFFFYLLDQILQAEIRNKPDIDLKRLLVQK 470

Mouse   467 FSKLLNDNIFHMSLLACALEVVMATYSRSTLQHLDSGTDLSFPWILNVLNLKAFDFYKVIESFIK 531
            .|.:    ||:::|:||.:|:|:..|.          |:|.|||:|:..::.||:|.|:||..::
  Fly   471 VSLV----IFNITLMACCVELVLEAYK----------TELKFPWVLDCFSISAFEFQKIIEIVVR 521

Mouse   532 ---VEANLTREMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDGEGPDNLEPACPLSLPLQGNH 593
               .|..|.|.:||||...|...:|.|||..:|.::::|..::           .||...|..|
  Fly   522 HGSHEGCLNRSLIKHLNSIEETCLERLAWARNSTVWEMIASAQ-----------LPLPTWLMVN- 574

Mouse   594 TAADMYLSPLRSPKKRTSTTRVNSAANTETQAASAFHTQKPLKSTSLALFYKKVYRLAYLRLNTL 658
              .|....||:                                     :|.:|||.|.:||:..|
  Fly   575 --LDRAAGPLQ-------------------------------------IFLRKVYLLGWLRIQKL 600

Mouse   659 CAR--LLSDHPELEHIIWTLFQHTLQNEYELMRDRHLDQIMMCSMYGICKVKNI-DLKFKIIVTA 720
            |:.  |....||   .||.:|:|::.:|.|||:||||||.:||::|...:||.: |.||..|:.|
  Fly   601 CSELSLCEKTPE---SIWHIFEHSITHETELMKDRHLDQNIMCAIYIYIRVKRMEDPKFSDIMRA 662

Mouse   721 YKDLPHAAQETFKRVLI-----REEEFDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPR 780
            |::.|.|....::.|.|     .|.:...||.|||..::..::..::.|.:..|........:..
  Fly   663 YRNQPQAVNSVYREVFIDINEDGEPKVKDIIHFYNHTYVPLMRQFVIDYLNVTPDVSGRASDLQL 727

Mouse   781 SPYKFSSSPLRIPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMV 845
            ||:.                        .|....|.|:|....:.||   ....:|..|..||||
  Fly   728 SPHP------------------------KERAAQPKKVTQSHSLFVS---QMSKNEIQQSPNQMV 765

Mouse   846 ---CNSDRVLKRSAEGGNPPKPLKKLRFDIEG-------ADEAD-GSKHLPAESKFQQKLAEMTS 899
               |.|            |.|.|:.:...:.|       .||.. |:......||..|..|.|..
  Fly   766 YSFCRS------------PAKDLQAMNEKVRGGKRMLSFGDEPGLGTMAETKRSKISQVKAVMDD 818

Mouse   900 TRTRMQKQR 908
            ...:..:|:
  Fly   819 PELQSAEQQ 827

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Rb1	NP_033055.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..36		6/11 (55%)
		DUF3452	112..221	CDD:463402	30/124 (24%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	341..360		4/18 (22%)
		Pocket, binds T and E1A. /evidence=ECO:0000250	367..764		109/420 (26%)
		Domain A. /evidence=ECO:0000250	367..573		55/221 (25%)
		RB_A	367..567	CDD:460364	54/215 (25%)
		Spacer. /evidence=ECO:0000250	574..632		7/57 (12%)
		Domain B. /evidence=ECO:0000250	633..764		47/138 (34%)
		CYCLIN_RB	638..764	CDD:410302	47/133 (35%)
		Interaction with LIMD1. /evidence=ECO:0000250	756..921		31/164 (19%)
		Rb_C	761..918	CDD:462639	31/159 (19%)
		Domain, mediates interaction with E4F1. /evidence=ECO:0000250	764..921		31/156 (20%)
		Bipartite nuclear localization signal. /evidence=ECO:0000269|PubMed:8336704	853..869		3/15 (20%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	872..921		10/45 (22%)
Rbf	NP_525036.2	DUF3452	90..228	CDD:463402	30/141 (21%)
		RB_A	377..560	CDD:460364	54/214 (25%)
		RB_B	582..705	CDD:460363	48/162 (30%)