DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Med1 and MED1

DIOPT Version :9

Sequence 1:	NP_001073587.1	Gene:	Med1 / 19014	MGIID:	1100846	Length:	1575	Species:	Mus musculus
Sequence 2:	NP_649341.1	Gene:	MED1 / 40403	FlyBaseID:	FBgn0037109	Length:	1475	Species:	Drosophila melanogaster

Alignment Length:	1671	Identity:	412/1671 - (24%)
Similarity:	661/1671 - (39%)	Gaps:	402/1671 - (24%)

- Green bases have known domain annotations that are detailed below.

Mouse     8 EDSERLSKMSSLLERLHAKF-NQNRPWSETIKLVRQ-VMEKRVVMSSGGHQHLVSCLETLQKALK 70
            |:..:..:..:::|:|.||: |:.:.:.|..|.||. .:||...:.......|.|.|:.||..:|
  Fly    21 EEKNKQIQQETMMEKLRAKYRNKPKSYEEIKKSVRMYFIEKHYPLDPLCKATLQSALDKLQHYIK 85

Mouse    71 VTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSC 135
            |||...:.:||||::||  ||..........:|::||||||:.||.||.|.||||.|..:.....
  Fly    86 VTSRHGLVERLESLSRQ--LGLKFMEDQQLLFISTDMFYVEILLDAAGSLSDVKVHHECKIEQQS 148

Mouse   136 PELVQQLREKNFEEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAIMYWKATNAAPL 200
            .|||..|:..:|.:|:..|:||.::|.|..:.|:|.|.::|||::|.|:..:..::.:..:....
  Fly   149 SELVACLKSGDFADFTVQLEGLSSIYQLNAEPKVKKKAFVALQAMETDIQSLYQLHLQGHSGDSY 213

Mouse   201 DKILHGSVGYLTPRSGGHLMNMKYYASPSDLL--DDKTASPIILHEKNVPRSLGMNASVTIEGTS 263
            ..:...|||.:.||.|||.|.:.|:..|..|.  |.|.||.....::.:..|.|::|::.:||:|
  Fly   214 SLMTSSSVGLVLPRRGGHPMRLTYFCPPLHLPEGDPKLASGDFTIDQVMRSSYGLSATINLEGSS 278

Mouse   264 A--MYKLPIAPLIMGSHPADNKWTPSFSAVTSANSVDLPACFFLKFPQPIPVSKAFVQKLQNCTG 326
            |  :..||...|:..:.....  .|:::.:...||:.:||.|.|:..:|:||....::.|    |
  Fly   279 ANKLQTLPTVTLVRDAQTGLE--VPTYAQLNQNNSLLMPATFVLRLNKPMPVCLESLKAL----G 337

Mouse   327 IPLFET------PPTYLPLYELITQFELSKDPDPLPLNHNMR-FYAALPGQQHCYFLNKDAPLPD 384
            :|..::      |||  .:..||.| ..||.    .:.:..| .|..||.:.||||..      |
  Fly   338 LPGLDSVATPPGPPT--TVLNLIVQ-TASKQ----AIKNTQRGLYVNLPKETHCYFFT------D 389

Mouse   385 GQSLQGTLVSKITFQHPGRVPLILNMIRHQVAYNTLIGSCVKRTILK-EDSPGLLQFEVCPLSES 448
            .:.|||||||.:.|..|.:||.|:..::.|..:.||:.|||:....: .|....:..||..:|.:
  Fly   390 NRKLQGTLVSSLPFTEPAQVPRIVAFLKKQALFYTLLASCVREQQKQYNDMDSTVILEVTAVSFN 454

Mouse   449 RFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALIC-TDDF------IAKVVQRCMSIPV 506
            :.:|..|||..:||..|...::|. ..:|.:|        | |:::      :.:..::.:|||:
  Fly   455 QITVELQHPYEESLATVDFLLEDG-QPTCSVY--------CLTNEYELLSQKLTRTARKVVSIPM 510

Mouse   507 TMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGY---GMTTGNNPMSG--------- 559
            .:..:             |....|..|..:...:.|.|..|.   |..:|..|:||
  Fly   511 VIYKL-------------LKCWDEEHEFKLHGAIGPGSGSGAIGGGGMSGGGPVSGVGNNFSQFS 562

Mouse   560 --TTTPTN-TFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSS 621
              |.||:: :.|||....:.|:.|..|.|                    ||........|.
  Fly   563 MDTPTPSDGSLPGGGFANINNLKMDAKSR--------------------SLADAFAASTSA---- 603

Mouse   622 PTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEMCSGSNK 686
                      .:::||       |:||.::...|..|:..|:: :...:|||||.:......::|
  Fly   604 ----------AAAIAG-------LINLKRETDPQSGSSASGTT-VSGSSSSSGSAKTSDHDIADK 650

Mouse   687 AKKKKSSRVP-----------PDKPKHQTED-----DFQRE--LFSMDVDSQNPMFDVSMTADAL 733
            .|.....:.|           |...|..:..     :.||.  :..:.:::|..:....:||.:.
  Fly   651 YKNIWKDKTPNLKHCVSITPIPGDGKSGSAGGVSGVEVQRTGGIEIIPLNAQAAIAGGGVTASSS 715

Mouse   734 DTP---HITPA----PSQCSTPPATYPQPVSHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEAS 791
            .||   .|||.    ||:.||..:|                  ::|..:|     :.:....|:|
  Fly   716 ATPTTITITPITGKDPSKDSTKKST------------------AASAGVG-----VAAKRPHESS 757

Mouse   792 KLPSTSDDCPPIGTPVRDSSSSGHSQSALFDSDVFQTNNNENPYTDPADLIADAAGSPNSDSPTN 856
            ...|::..|...|:.:..|:|||.|.:        |.........|              |||..
  Fly   758 TSSSSTSGCSGSGSSMSSSASSGSSDT--------QKEKKRKKKRD--------------DSPMG 800

Mouse   857 HFFPDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGMPMLGGDN-----G 916
                     .|:.:.|:..|..|..  |.|:..|....|...:|.       |..||..     |
  Fly   801 ---------PPEKIYSRQNSPAGGA--DASATGGVVRKFSSPSSS-------PKAGGGGQGLMAG 847

Mouse   917 EPKFKGSSQADTVDFSIISVAGKALGAADLMEHHSGSQSPLLTTGELGKEKTQKRVKEGNGTGAS 981
            .|..:.|.:...|..|              .:|::.|.||                |...||.:.
  Fly   848 VPTARPSPKHSPVYSS--------------PKHNTASNSP----------------KSPFGTHSP 882

Mouse   982 SGSGPGSDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSS 1046
            .   .||..||...:...:....:...|...|.:...|.              ||:|.|.|.|||
  Fly   883 K---HGSSGKPSMSTLKSAATAATILSPKGDKSSSAVGN--------------TSSGPSASSGSS 930

Mouse  1047 G-----RSQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYTSSGSVSSSGS--KSHHSHSSSS 1104
            |     ||....|...||.|     ||        |.:    :||||:|||.|  |...|.:|.|
  Fly   931 GATGLVRSFASVGAPPPPPP-----IP--------PLA----SSSGSISSSQSLKKEKTSSASGS 978

Mouse  1105 SSLASASTSG-----------------KVKSSKSEGSSSSKLS--------GSMYAS---QGSSG 1141
            ||.:|::|:|                 .:|||:.:..|.:.||        || ||:   .|:||
  Fly   979 SSTSSSATAGVASGGGISPASVAAAVAALKSSQQQMKSVASLSHLAAGGGLGS-YAAPSGAGASG 1042

Mouse  1142 SSQSKNSSQTGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSISKPNI-SPSHSRPPGGSD 1205
            ::.....:..|...|:|.:....|..|.:.      |..|.:.:.:...|.| :|:.:...|.:.
  Fly  1043 AAAVVVGAGAGAGAGASGLELSALRKGMAG------GAVSLMTSTAALAPTIPAPTTTVAAGSAA 1101

Mouse  1206 KLASPM---------------------------KPVPGTPPSSK--AKSPISSGSSGS-HVSGTS 1240
            .|.||:                           :|.||..|:|.  ..|..||.|:|| :.:|.|
  Fly  1102 SLVSPVSAVVGQGQETAGAAAAATLATATILQQQPQPGAAPTSSCLTTSGGSSDSAGSINPAGAS 1166

Mouse  1241 SSSGMKSSSGSASSGSVSQKTPPASNSCTPSSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSL 1305
            :...:|.||......::: ||..:.:|.|.|.|| ||||....:..:..|::.....:.:.|...
  Fly  1167 TEYMVKPSSQEGLKLTIN-KTGSSKSSGTGSGSS-SSSGLQAKAKSSSSGATSFAGSTGSTKKQH 1229

Mouse  1306 TAVIDKLKHGVVTSGPGGEDPIDSQMGASTNSSNHPMSSKHNTSGGEFQSKREKSDKDKSKVSAS 1370
            |.    ||.| |.|||..:....:...|:.:||.| ...|.|:||         :...|...|.|
  Fly  1230 TG----LKPG-VNSGPASKKATAAVSSATASSSKH-FFQKANSSG---------NLSSKLSGSGS 1279

Mouse  1371 GGSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESGGDGLRPQIASSKNYGS 1435
            ||.:..:|..|.:             |..:..:|..:..:.....|.|||...:.::.|:...||
  Fly  1280 GGGIPLTKSNSTN-------------SFQEHNAPRRRPSMGALASGSSGGGSGQRKLGSASGGGS 1331

Mouse  1436 PLISGSTPKHERGSPSHSKSPAYTPQNVDSESESGSSIAERSYQNSPSSEDGIRPLPEYSTEKHK 1500
            . .|||.      ||:.|.|.:..|...|..::..:.:...|...:.|.|..|:.|       |.
  Fly  1332 G-SSGSV------SPALSGSMSQPPPRFDHHTDMMTILQYASPTMAASMEGFIKGL-------HN 1382

Mouse  1501 KHKKEKKKVRDKDRDKKKSHSMKPENWSKSPISSDPTASVTNNPIL 1546
            |.:..|...|....:.....| .|..      ||:|..:.|::.||
  Fly  1383 KFQIPKLSQRGSGGNTTSGRS-TPSG------SSEPALAGTSSSIL 1421

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Med1	NP_001073587.1	Interaction with the Mediator complex and THRA. /evidence=ECO:0000250	1..670		190/697 (27%)
		Interaction with ESR1. /evidence=ECO:0000250\|UniProtKB:Q15648	16..590		178/609 (29%)
		Med1	60..426	CDD:287688	125/376 (33%)
		Interaction with the Mediator complex. /evidence=ECO:0000250	108..212		36/103 (35%)
		Interaction with the Mediator complex. /evidence=ECO:0000250	215..390		52/185 (28%)
		Interaction with THRA. /evidence=ECO:0000250	405..644		53/261 (20%)
		Interaction with VDR. /evidence=ECO:0000250	542..789		56/286 (20%)
		LXXLL motif 1	604..608		1/3 (33%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	609..706		19/107 (18%)
		Interaction with GATA1. /evidence=ECO:0000269\|PubMed:17132730	622..701		17/89 (19%)
		Interaction with PPARGC1A and THRA. /evidence=ECO:0000250	622..701		17/89 (19%)
		LXXLL motif 2	645..649		2/3 (67%)
		Interaction with ESR1. /evidence=ECO:0000250	656..1066		90/444 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	737..760		8/26 (31%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	791..818		9/26 (35%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	874..895		5/20 (25%)
		Integrase domain-binding motif (IBM). /evidence=ECO:0000250\|UniProtKB:Q15648	875..902		7/26 (27%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	951..1564		162/662 (24%)
		Interaction with TP53. /evidence=ECO:0000250	1251..1423		42/171 (25%)
MED1	NP_649341.1	Med1	75..431	CDD:287688	125/376 (33%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167838334
Domainoid	1	1.000	185	1.000	Domainoid score	I3385
eggNOG	1	0.900	-	-		E1_28HMI
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	264	1.000	Inparanoid score	I3062
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG47792
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0005568
OrthoInspector	1	1.000	-	-		oto92755
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	LDO	PTHR12881
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R4643
SonicParanoid	1	1.000	-	-		X5411
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	13	12.890