DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Sqstm1 and ref(2)P

DIOPT Version :9

Sequence 1:	NP_035148.1	Gene:	Sqstm1 / 18412	MGIID:	107931	Length:	442	Species:	Mus musculus
Sequence 2:	NP_001014491.1	Gene:	ref(2)P / 35246	FlyBaseID:	FBgn0003231	Length:	599	Species:	Drosophila melanogaster

Alignment Length:	513	Identity:	113/513 - (22%)
Similarity:	171/513 - (33%)	Gaps:	181/513 - (35%)

- Green bases have known domain annotations that are detailed below.

Mouse    99 IKEKKECRREHRPPCAQ----EAPRNM-VHPNVICDGCN-GPVVGTRYKCSVCPDYDLCSVCEGK 157
            ::|.|..::|.....|:    :.|.|. :|..|.||||. .|::|.||||..|.:||||..||..
  Fly    93 VEEPKATKQEGSSANAEAPSVDDPSNFTIHDAVECDGCGLAPLIGFRYKCVQCSNYDLCQKCELA 157

Mouse   158 GLHREHSKLIFPN----------------------------PFGHLS------DSFSHSRWLRKL 188
            ..|.||..|..|.                            ||...:      :....||..|:.
  Fly   158 HKHPEHLMLRMPTNNGPGMVDAWFTGPGLGRRSGRRSRGHCPFQETNQADPAGEPARDSRRERRQ 222

Mouse   189 KHGHFG----WPGWEMGPPGNWS-------PRPPRAGD-------GRPCPTAESA---------- 225
            ...|.|    :.......|.|.:       |:.|:|.:       ..|..|||.|
  Fly   223 ARRHAGVLTQFVEMMTNLPLNTTTATAPAEPQKPKAAEQTESPPQAEPTVTAEKAAESEAKPTEP 287

Mouse   226 --------------------SAPPEDPNVNFLKNVGESVAAALSPLGIEV----------DIDVE 260
                                |..|..|.:| |.|:.:.|.......|||:          .||..
  Fly   288 KKVNTDQSVPRTEDPVTTPRSTQPTTPVIN-LDNISQIVPPEYMSAGIEILNNFSEMFSKIIDTT 351

Mouse   261 HGGKRSRLTP-TTPESSSTGTEDKSNTQ-----------------PSSCSSEV------SKPDGA 301
            .||......| |||.:.:...|::..:.                 ||:..|.|      :.|..:
  Fly   352 EGGDSGIFAPSTTPSAENKKPEEQGQSSGQSGASSANQSAVPSAAPSANQSNVPSANQSATPSIS 416

Mouse   302 GEGP-AQSLTEQMKKIALESVGQPEEQMESGNCSGGDDDW-------------------THLSSK 346
            |..| ||..||.:.....|:..:.|::....:..  |.:|                   |:.::.
  Fly   417 GSIPDAQLETEPLNPKPSETTTETEQERRRSDSL--DPEWQLIDNAYSANNSNLINLDTTNPTAA 479

Mouse   347 EVDP-----STGELQSLQMPE--------------------------SEGPSSLDPSQEGPTGLK 380
            ..:|     ..|||....|.|                          |...:|:..|...|...:
  Fly   480 PQEPVRDFGQLGELLRQHMNEEARVEQASANTQTAQVDTVSTSTSTTSVTTNSVGTSPAAPDDKR 544

Mouse   381 EAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKH 438
            ...:|     ..|..:.:|:..|::||||:||.|||:||::...:|.||||.:..|::
  Fly   545 TVPVY-----HTDESINKSIHAMMAMGFSNEGAWLTQLLESVQGNISAALDVMNVSQN 597

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Sqstm1	NP_035148.1	Interaction with LCK. /evidence=ECO:0000250\|UniProtKB:Q13501	2..50
		PB1_p62	5..102	CDD:99723	0/2 (0%)
		Interaction with PRKCZ and dimerization. /evidence=ECO:0000250\|UniProtKB:O08623	43..107		2/7 (29%)
		Interaction with PAWR. /evidence=ECO:0000250\|UniProtKB:Q13501	50..80
		Interaction with GABRR3. /evidence=ECO:0000250\|UniProtKB:O08623	122..224		39/154 (25%)
		ZZ_NBR1_like	126..166	CDD:239080	21/40 (53%)
		LIM protein-binding. /evidence=ECO:0000250\|UniProtKB:Q13501	170..220		13/101 (13%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	204..234		12/73 (16%)
		TRAF6-binding. /evidence=ECO:0000250\|UniProtKB:Q13501	228..233		1/4 (25%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	262..308		15/70 (21%)
		Interaction with NTRK1. /evidence=ECO:0000250\|UniProtKB:O08623	269..442		52/245 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	321..386		14/114 (12%)
		MAP1LC3B-binding. /evidence=ECO:0000250\|UniProtKB:Q13501	323..344		4/39 (10%)
		LIR	338..343		2/23 (9%)
		Interaction with KEAP1. /evidence=ECO:0000269\|PubMed:20173742, ECO:0000269\|PubMed:20421418	349..354		1/9 (11%)
		UBA_5	381..442	CDD:318725	21/58 (36%)
ref(2)P	NP_001014491.1	PB1	6..88	CDD:295447
		ZZ	121..165	CDD:278966	22/43 (51%)
		UBA_SQSTM	555..593	CDD:270505	18/37 (49%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167833630
Domainoid	1	1.000	44	1.000	Domainoid score	I12268
eggNOG	1	0.900	-	-		E1_KOG4582
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	1	0.950	-	0	Normalized mean entropy	S7489
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0002104
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	LDO	PTHR15090
Phylome	0	0.000	Not matched by this tool.
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R4736
SonicParanoid	1	1.000	-	-		X5995
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	8	7.910