Protein Alignment Nos2 and Nos

DIOPT Version :10

• Sequence 1: NP_035057.1
• Gene: Nos2 / 18126
• MGIID: 97361
• Length: 1144
• Species: Mus musculus
• Sequence 2: NP_523541.2
• Gene: Nos / 34495
• FlyBaseID: FBgn0011676
• Length: 1349
• Species: Drosophila melanogaster
• Alignment Length: 1119
• Identity: 508/1119 - (45%)
• Similarity: 682/1119 - (60%)
• Gaps: 84/1119 - (7%)

Mouse    79 VRIKNWGSGEILHDTLHHKATSDFTCKSKSCLGSIMNPKSLTRGPRDKPTPLEELLPHAIEFINQ 143
            :|:||......::||||.|.....:|...:|..||||..:.....|..    :.:|.||.:|:.|
  Fly   217 MRLKNIEGRPEVYDTLHCKGREILSCSKATCTSSIMNIGNAAVEARKS----DLILEHAKDFLEQ 277

Mouse   144 YYGSFKEAKIEEHLARLEAVTKEIETTGTYQLTLDELIFATKMAWRNAPRCIGRIQWSNLQVFDA 208
            |:.|.|......|..|.:.|.:.|||||.||||..|||:..|:||||:.|||||||||.|||||.
  Fly   278 YFTSIKRTSSTAHETRWKQVRQSIETTGHYQLTETELIYGAKLAWRNSSRCIGRIQWSKLQVFDC 342

Mouse   209 RNCSTAQEMFQHICRHILYATNNGNIRSAITVFPQRSDGKHDFRLWNSQLIRYAGYQMPDGTIRG 273
            |..:|...||:.||.||.||||.||:|||||:||||:|.|||:|:||:|||.||||:..||.|.|
  Fly   343 RYVTTTSGMFEAICNHIKYATNKGNLRSAITIFPQRTDAKHDYRIWNNQLISYAGYKQADGKIIG 407

Mouse   274 DAATLEFTQLCIDLGWKPRYGRFDVLPLVLQADGQDPEVFEIPPDLVLEVTMEHPKYEWFQELGL 338
            |...:|||::|..||||.:...:|:||||:.|:|.||:.|:.||:|:|||.:.|||:|||.:|||
  Fly   408 DPMNVEFTEVCTKLGWKSKGSEWDILPLVVSANGHDPDYFDYPPELILEVPLTHPKFEWFSDLGL 472

Mouse   339 KWYALPAVANMLLEVGGLEFPACPFNGWYMGTEIGVRDFCDTQRYNILEEVGRRMGLETHTLASL 403
            :|||||||::||.:|||::|.|..|:||||.||||.|:.|||.|.|:||.|..:|.|:|.|..||
  Fly   473 RWYALPAVSSMLFDVGGIQFTATTFSGWYMSTEIGSRNLCDTNRRNMLETVALKMQLDTRTPTSL 537

Mouse   404 WKDRAVTEINVAVLHSFQKQNVTIMDHHTASESFMKHMQNEYRARGGCPADWIWLVPPVSGSITP 468
            |||:||.|:|:|||||:|.:||||:||||||||||||.:||.:.|.||||||||:|||:||||||
  Fly   538 WKDKAVVEMNIAVLHSYQSRNVTIVDHHTASESFMKHFENESKLRNGCPADWIWIVPPLSGSITP 602

Mouse   469 VFHQEMLNYVLSPFYYYQIEPWKTHIWQ----NEKLRPRRREIRFRVLVKVVFFASMLMRKVMAS 529
            ||||||..|.|.|.:.||...|:||:|:    ..|.:..||:..|:.:.:.|.|.|.|..:.::.
  Fly   603 VFHQEMALYYLKPSFEYQDPAWRTHVWKKGRGESKGKKPRRKFNFKQIARAVKFTSKLFGRALSK 667

Mouse   530 RVRATVLFATETGKSEALARDLATLFSYAFNTKVVCMDQYKASTLEEEQLLLVVTSTFGNGDCPS 594
            |::||||:||||||||..|:.|..|..:|||.::.||..|..|::|.|.||:||.|||||||.|.
  Fly   668 RIKATVLYATETGKSEQYAKQLCELLGHAFNAQIYCMSDYDISSIEHEALLIVVASTFGNGDPPE 732

Mouse   595 NGQTLKKSLFMLR------------------------------------------------ELNH 611
            ||:...:.|:.:|                                                ..:.
  Fly   733 NGELFSQELYAMRVQESSEHGLQDSSIGSSKSFMKASSRQEFMKLPLQQVKRIDRWDSLRGSTSD 797

Mouse   612 TF-----------RYAVFGLGSSMYPQFCAFAHDIDQKLSHLGASQLAPTGEGDELSGQEDAFRS 665
            ||           |:|||.||||.||.||||...:|..|..||..:|.....|||:.|||.:||.
  Fly   798 TFTEETFGPLSNVRFAVFALGSSAYPNFCAFGQYVDNILGELGGERLLRVAYGDEMCGQEQSFRK 862

Mouse   666 WAVQTFRAACETFDVRSKHHIQIPKRFTSNATWEPQQYRLIQSPEPLDLNRALSSIHAKNVFTMR 730
            ||.:.|:.|||||.:..:..:........|.:......||:.|.....|:.:||..|.|.|...:
  Fly   863 WAPEVFKLACETFCLDPEESLSDASLALQNDSLTVNTVRLVPSANKGSLDSSLSKYHNKKVHCCK 927

Mouse   731 LKSQ-QNL-QSEKSSRTTLLVQLTFEGSRGPSYLPGEHLGIFPGNQTALVQGILERVVDCPTPHQ 793
            .|:: .|| :..:.::||:|:::.   :.|..|.||:|:||||.|:|.||.|:|.|:|....|.:
  Fly   928 AKAKPHNLTRLSEGAKTTMLLEIC---APGLEYEPGDHVGIFPANRTELVDGLLNRLVGVDNPDE 989

Mouse   794 TVCLEVLDE----SGSY--WVKDKRLPPCSLSQALTYFLDITTPPTQLQLHKLARFATDETDRQR 852
            .:.|::|.|    :|.:  |....::||.:|...|..|.|:||||::..|..||.|..|..|::|
  Fly   990 VLQLQLLKEKQTSNGIFKCWEPHDKIPPDTLRNLLARFFDLTTPPSRQLLTLLAGFCEDTADKER 1054

Mouse   853 LEALCQPSE-YNDWKFSNNPTFLEVLEEFPSLHVPAAFLLSQLPILKPRYYSISSSQDHTPSEVH 916
            ||.|...|. |.||:....|..|:|||||||...||..||:||..|:||:||||||......|:|
  Fly  1055 LELLVNDSSAYEDWRHWRLPHLLDVLEEFPSCRPPAPLLLAQLTPLQPRFYSISSSPRRVSDEIH 1119

Mouse   917 LTVAVVTYRTRDGQGPLHHGVCSTWIRNLKPQDPVPCFVRSVSGFQLPEDPSQPCILIGPGTGIA 981
            ||||:|.||..||||...:||||.::..|:..|.:..||||..||.||.|.|:|.||||||||||
  Fly  1120 LTVAIVKYRCEDGQGDERYGVCSNYLSGLRADDELFMFVRSALGFHLPSDRSRPIILIGPGTGIA 1184

Mouse   982 PFRSFWQ--QRLHDSQHKGLKGGRMSLVFGCRHPEEDHLYQEEMQEMVRKRVLFQVHTGYSRLPG 1044
            |||||||  |.|.|..... |..:|.|.||||:.:.| ||.||..|:.:.::|.:|....||...
  Fly  1185 PFRSFWQEFQVLSDLDPTA-KLPKMWLFFGCRNRDVD-LYAEEKAELQKDQILDRVFLALSREQA 1247

Mouse  1045 KPKVYVQDILQKQLANEVLSVLHGEQGHLYICGDVRMARDVATTLKKLVATKLNLSEEQVEDYFF 1109
            .||.||||:::::. :.:..::..|:||:|:||||.||..|..|::|.:|.|...||.:||.:..
  Fly  1248 IPKTYVQDLIEQEF-DSLYQLIVQERGHIYVCGDVTMAEHVYQTIRKCIAGKEQKSEAEVETFLL 1311

Mouse  1110 QLKSQKRYHEDIFG 1123
            .|:.:.||||||||
  Fly  1312 TLRDESRYHEDIFG 1325

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Nos2	NP_035057.1	DINNN-motif, mediates interaction with SPSB1, SPSB2 and SPSB4. /evidence=ECO:0000269|PubMed:20603330	23..27
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	37..59
		NOS_oxygenase_euk	78..490	CDD:238410	230/410 (56%)
		Calmodulin-binding. /evidence=ECO:0000250|UniProtKB:P35228	509..529		5/19 (26%)
		Flavodoxin_1	535..666	CDD:425562	67/189 (35%)
		FNR_like	730..1126	CDD:447143	178/405 (44%)
Nos	NP_523541.2	NOS_oxygenase_euk	216..624	CDD:238410	230/410 (56%)
		Flavodoxin_1	673..863	CDD:425562	67/189 (35%)
		Nitric_oxide_synthase	931..1328	CDD:99799	177/401 (44%)