Protein Alignment Nek2 and Nek2

DIOPT Version :10

• Sequence 1: NP_035022.2
• Gene: Nek2 / 18005
• MGIID: 109359
• Length: 443
• Species: Mus musculus
• Sequence 2: NP_572415.1
• Gene: Nek2 / 31696
• FlyBaseID: FBgn0029970
• Length: 735
• Species: Drosophila melanogaster
• Alignment Length: 492
• Identity: 177/492 - (35%)
• Similarity: 258/492 - (52%)
• Gaps: 82/492 - (16%)

Mouse     5 VEDYEVLHSIGTGSYGRCQKIRRKSDGKILVWKELDYGSMTEVEKQMLVSEVNLLRELKHPNIVS 69
            ::|||||..:|.||:|.|.|:|.||.|::..||.::|..:.|.:...||||:::||:|:|||||.
  Fly    16 LQDYEVLAVMGNGSFGTCYKVRDKSTGELFAWKGMNYDELDEAKCDALVSEISVLRQLQHPNIVQ 80

Mouse    70 YYDRIIDRTNTTLYIVMEYCEGGDLASVISKGTKDRQYLEEEFVLRVMTQLTLALKECHRRSDGG 134
            ||..:::|...::|||||.|.|||||.::.:....||..||.::.||:.||..||:.||.:...|
  Fly    81 YYHHLVNREAKSVYIVMECCAGGDLAQIVQRARSQRQRFEEPYIWRVLFQLCRALQVCHNKIPNG 145

Mouse   135 HTVLHRDLKPANVFLDSKHNVKLGDFGLARILNHDTSFAKTFVGTPYYMSPEQMSCLSYNEKSDI 199
             |:||||:||||:|||:..|.|||||||||:|..|.|||.:|||||:|||||.:....|:.|||:
  Fly   146 -TILHRDIKPANIFLDAAGNAKLGDFGLARMLRRDQSFAASFVGTPHYMSPELVKGRKYDRKSDV 209

Mouse   200 WSLGCLLYELCALMPPF--TAFNQKELAGKIREGRFRRIPYRYSDGLNDLITRMLNLKDYHRPSV 262
            |::|||:||:|||.|||  .||:|  |:.||.:|.|.|||..||..|.::|..||.:....||.:
  Fly   210 WAVGCLVYEMCALRPPFRGRAFDQ--LSEKIAQGEFSRIPAIYSTDLQEIIAFMLAVDHEQRPGI 272

Mouse   263 EEILESPLIADLVAE-------------------------------------------EQRRNLE 284
            |.|:..||:...::|                                           ||....|
  Fly   273 EVIIRHPLVVRNISELDGKFPILVDSGEDFYTLPSGARLFEDEEEDGVHPELSSTMFTEQYSFNE 337

Mouse   285 RRGRRSGEPSKL--PDSSPVLSEL---------KLKERQLQDRE--QALRAREDILEQKERELCI 336
            ..|:|....:.:  ||   :.|||         ..|:.||.|..  :::| ||:..|:::.||..
  Fly   338 GYGQRRLSVTGVFTPD---LRSELFYSAKRKIFPAKKLQLSDPSLYESIR-REERAEERQVELAE 398

Mouse   337 RERLAEDKLARAESLMKNYSLLKEHRLLCLAGGPELDLPSSAMKKKVHFHGESKENTARSENSES 401
            ..|..:|:    |...::..||||             .|||......:...|..:....:..::.
  Fly   399 ERRRKKDQ----EQQKRDQELLKE-------------APSSPRALTQNIFDEVLKTRLHAIRAQE 446

Mouse   402 YLAKSKCRDLKKRLHAAQLRAQALADIEKNYQLKSRQ 438
            .|.:.|..:|:.|....||..|.:..:|:..|.|..|
  Fly   447 SLLQQKLEELQTREQELQLAEQRVQTLERQMQEKLLQ 483

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Nek2	NP_035022.2	STKc_Nek2	7..271	CDD:270857	133/265 (50%)
		Interaction with PCNT. /evidence=ECO:0000250	264..443		46/231 (20%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	282..303		5/22 (23%)
		Interaction with CEP85. /evidence=ECO:0000250|UniProtKB:P51955	301..443		35/149 (23%)
		Leucine-zipper	306..334		9/38 (24%)
		Necessary for interaction with MAD1L1. /evidence=ECO:0000250	329..443		24/110 (22%)
		Required for microtubule binding and for localization to the centrosomes. /evidence=ECO:0000250	333..370		9/36 (25%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	383..402		1/18 (6%)
		Interaction with SAV1 and STK3/MST2. /evidence=ECO:0000250	402..437		10/34 (29%)
Nek2	NP_572415.1	STKc_Nek2	18..281	CDD:270857	132/264 (50%)