Protein Alignment Smad4 and Mad

DIOPT Version :10

• Sequence 1: NP_032566.2
• Gene: Smad4 / 17128
• MGIID: 894293
• Length: 551
• Species: Mus musculus
• Sequence 2: NP_001259992.1
• Gene: Mad / 33529
• FlyBaseID: FBgn0011648
• Length: 525
• Species: Drosophila melanogaster
• Alignment Length: 553
• Identity: 194/553 - (35%)
• Similarity: 258/553 - (46%)
• Gaps: 166/553 - (30%)

Mouse    20 VHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQV 84
            |..|:..:||.|.|.:|::|::|||||||::|..::.|..|::..| .|||||||.|:|||||||
  Fly    98 VKKLLGWKQGDEEEKWAEKAVDSLVKKLKKRKGAIEELERALSCPG-QPSKCVTIPRSLDGRLQV 161

Mouse    85 AGRKGFPHVIYARLWRWPDLHK-NELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTL 148
            :.|||.|||||.|:||||||.. :|||.::.|||.|..|...||:|||||:||.||.:
  Fly   162 SHRKGLPHVIYCRVWRWPDLQSHHELKPLELCQYPFSAKQKEVCINPYHYKRVESPVL------- 219

Mouse   149 QSNAPSMLV--KDEYVHDFEGQPSLPTEGHSIQTIQHPPSNRASTETYSAPALLAPAESNATSTT 211
                |.:||  ..|:           ..|||:....|
  Fly   220 ----PPVLVPRHSEF-----------APGHSMLQFNH---------------------------- 241

Mouse   212 NFPNIPVASTSQPASILAGSHSEGLLQIASGPQPGQQQNGFTAQPATYHHNSTT-TWTGSRTAPY 275
                  ||..|.|.::   |:|               .:||.:     |..||: |..||.::..
  Fly   242 ------VAEPSMPHNV---SYS---------------NSGFNS-----HSLSTSNTSVGSPSSVN 277

Mouse   276 T-PNLPHHQNGHLQHHPP---MPPHPGHYWPVHNELAFQPPISNHP------------------- 317
            : ||.|:..   |...||   .|...|:              ||:|
  Fly   278 SNPNSPYDS---LAGTPPPAYSPSEDGN--------------SNNPNDGGQLLDAQMGDVAQVSY 325

Mouse   318 -APEYWCSIAYFEMDVQVGETFKVPSSCPVVTVDGYVDPS-GGDRFCLGQLSNVHRTEAIERARL 380
             .|.:|.||||:|::.:|||.|...::.  |.|||:.:|| ..||.||||||||:|...||..|.
  Fly   326 SEPAFWASIAYYELNCRVGEVFHCNNNS--VIVDGFTNPSNNSDRCCLGQLSNVNRNSTIENTRR 388

Mouse   381 HIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQ 445
            ||||||.|... .|:|:..||||.|:||||...:...|..| ..|.||.|...:|:|:.::
  Fly   389 HIGKGVHLYYV-TGEVYAECLSDSAIFVQSRNCNYHHGFHP-STVCKIPPGCSLKIFNNQE---- 447

Mouse   446 MQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGI-GVDDLRRLCILRMSFVKGWG 509
                                           .|..:|.|...|. .|.:|.::|.:|||||||||
  Fly   448 -------------------------------FAQLLSQSVNNGFEAVYELTKMCTIRMSFVKGWG 481

Mouse   510 PDYPRQSIKETPCWIEIHLHRALQLLDEVLHTM 542
            .:|.||.:..||||||||||..||.||:||..|
  Fly   482 AEYHRQDVTSTPCWIEIHLHGPLQWLDKVLTQM 514

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Smad4	NP_032566.2	Mediates interaction with ZBTB7A. /evidence=ECO:0000250|UniProtKB:Q13485	1..321		102/328 (31%)
		MH1_SMAD_4	14..138	CDD:199816	66/118 (56%)
		Required for interaction with TSC22D1. /evidence=ECO:0000250|UniProtKB:Q13485	44..69		9/24 (38%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	166..192		4/25 (16%)
		SAD	274..319		11/68 (16%)
		MH2_SMAD_4	319..540	CDD:199823	91/222 (41%)
Mad	NP_001259992.1	MH1_SMAD_1_5_9	93..216	CDD:199814	66/118 (56%)
		MH2_SMAD_1_5_9	325..525	CDD:199822	93/229 (41%)