Protein Alignment DHX36 and CG3225

DIOPT Version :10

• Sequence 1: NP_065916.2
• Gene: DHX36 / 170506
• HGNCID: 14410
• Length: 1008
• Species: Homo sapiens
• Sequence 2: NP_608860.1
• Gene: CG3225 / 33680
• FlyBaseID: FBgn0031631
• Length: 678
• Species: Drosophila melanogaster
• Alignment Length: 764
• Identity: 222/764 - (29%)
• Similarity: 360/764 - (47%)
• Gaps: 140/764 - (18%)

Human   165 RDSEYLLQENEPDGTLDQKLLEDLQKKKNDLRYIEMQHFREKLPSYGMQKELVNLIDNHQVTVIS 229
            :.|.::...|...|.::|                     ||:||....:.:::..::.|||.::.
  Fly    26 QSSAFVFNVNHNMGLMEQ---------------------RERLPIRQYRDQILYCLEKHQVVILV 69

Human   230 GETGCGKTTQVTQFILDNYIERGKGSACRIVCTQPRRISAISVAERVAAERAESCGSGNSTGYQI 294
            ||||.||:|||.|::    .|.|..:...|..|:|||:|.:::|.|||.||.|..  |::.||.:
  Fly    70 GETGSGKSTQVPQYL----YEWGWHTKGLIGITEPRRVSTVTLANRVAQERGELV--GDTVGYVV 128

Human   295 RLQSRLPRKQGSILYCTTGIILQWLQSDPYLSSVSHIVLDEIHERNLQSDVLMTVVKDLLNFRSD 359
            |....: .....|.:.|.||:|:.:.:||.|:....|::||.||||:.:|:::.::|.:|..||.
  Fly   129 RFLESM-SSCTKIKFMTEGILLREVLADPLLTQYGVIIVDEAHERNMLTDMILGLLKKILRKRSS 192

Human   360 LKVILMSATLNAEKFSEYF-----GNCPM-IHIPGFTFPVVEYLLEDVIEKIRYVPEQKEHRSQF 418
            ||:|:.|||::|..|||:|     |...: :.|.|...||..:.|
  Fly   193 LKLIISSATIDASFFSEFFSWPGSGEVSVKLSIEGRMHPVSNFYL-------------------- 237

Human   419 KRGFMQGHVNRQEKEEKEAIYKERWPDYVRELRRRYSASTVDVIEMMEDDKVDLNLIVALIRYIV 483
                                 .|...|||:|        ||:.:..:..
  Fly   238 ---------------------NEPCADYVKE--------TVETVWKLHQ---------------- 257

Human   484 LEEEDGAILVFLPGWDNISTLHDLLMSQVMFKSDKFL-IIPLHSLMPTVNQTQVFKRTPPGVRKI 547
             :|..|.||.||.|.:.:....|||...:.....:.| ::|::..|.:.:|..||...|.|.||:
  Fly   258 -KEPPGDILAFLTGQEEVLEALDLLREYIASSEQENLKVLPMYGSMSSTDQLSVFFTPPKGTRKV 321

Human   548 VIATNIAETSITIDDVVYVIDGGKIKETHFDTQNNISTMSAEWVSKANAKQRKGRAGRVQPGHCY 612
            |:|||||||||||..:|||||.|.:|...::.:....::....||||:|.||.|||||::||..|
  Fly   322 VLATNIAETSITIPGIVYVIDCGYVKVKWYNPKTCSDSLVIVPVSKASAIQRAGRAGRMRPGKVY 386

Human   613 HLYNGLRASLLDDYQLPEILRTPLEELCLQIKILRLGGIAYFLSRLMDPPSNEAVLLSIRHLMEL 677
            .||.......|...|.||:.|:.|....||:|.|.:|.|..|  ....||..:.:|.::..|..|
  Fly   387 RLYTKSDYEALAPRQPPEMRRSELSGAILQLKALGIGNILRF--DFPSPPPAQNLLSALESLFAL 449

Human   678 NALDKQEELT-PLGVHLARLPVEPHIGKMILFGALFCCLDPVLTIAASLSFKDPFVIP---LGKE 738
            :|:|:|..|| |:|..||.||....:.||:.......|.:.::||.|.|..:..|..|   :.::
  Fly   450 DAIDEQGNLTKPVGYLLAELPFSAMLSKMLYVSGQMGCSEEIITIIALLQVQSIFSRPASAVAQQ 514

Human   739 KIADARRK-ELAKDTRSDHLTVVNAFEGWEEARRRGFRYEKDYCWEYFLSSNTLQMLHNMKGQFA 802
            ....|.|| |:|:   .|.:|::||:.|:.|   .|.  .|::|.:|||....|:..|:::.|..
  Fly   515 SGRIAHRKFEVAE---GDFITMLNAYTGFVE---EGM--TKEFCGQYFLIYRNLKRAHSLREQLI 571

Human   803 EHLLGAGFVSSRNPKDPESNINSDNEKIIKAVICAGLYPKVAKIRLNLGKKRKMVKVYTKTDGLV 867
            .       |:.:....|..:...|.||:.|. |.||.:.:||.:. :.|..|   ::.:.|:  :
  Fly   572 T-------VARKKYGIPIFSCKGDVEKLCKC-ITAGFFTQVAYLH-HSGVYR---QISSGTE--L 622

Human   868 AVHPKSVNVEQTDFHYNWLIYHLKMRTSSIYLYD--------CTEVSPY 908
            |:||.|.........|  ::|...::|:.:::..        .||::|:
  Fly   623 AIHPNSTLYTLPQAQY--VVYGELLQTTKLFMNYVTVIKREWLTELAPH 669

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
DHX36	NP_065916.2	Necessary for nuclear and nucleolar caps localizations. /evidence=ECO:0000269|PubMed:18279852	1..200		4/34 (12%)
		Required for the pre-miR-134 transport. /evidence=ECO:0000250|UniProtKB:D4A2Z8	1..104
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..58
		Required for recruitment to cytoplasmic stress granules. /evidence=ECO:0000269|PubMed:18854321	1..51
		Required for G4-DNA-and G4-RNA-binding. /evidence=ECO:0000269|PubMed:21149580, ECO:0000269|PubMed:21846770, ECO:0000269|PubMed:24151078	53..105
		DSM (DHX36-specific motif). /evidence=ECO:0000269|PubMed:20472641, ECO:0000269|PubMed:22238380, ECO:0000269|PubMed:24369427, ECO:0000269|PubMed:26195789, ECO:0000269|PubMed:26649896	53..75
		RecA-like domain 1. /evidence=ECO:0000250|UniProtKB:Q05B79	106..386		72/226 (32%)
		DEXHc_DHX36	207..386	CDD:350739	66/184 (36%)
		HrpA	215..>776	CDD:441249	182/572 (32%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250|UniProtKB:Q05B79	265..317		18/51 (35%)
		DEAH box. /evidence=ECO:0000255|PROSITE-ProRule:PRU00541	334..337		2/2 (100%)
		RecA-like domain 2. /evidence=ECO:0000250|UniProtKB:Q05B79	387..628		68/241 (28%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250|UniProtKB:Q05B79	498..557		20/59 (34%)
		Nuclear localization signal. /evidence=ECO:0000303|PubMed:14731398	517..528		2/11 (18%)
		WH domain. /evidence=ECO:0000250|UniProtKB:Q05B79	629..698		26/69 (38%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250|UniProtKB:Q05B79	638..697		21/59 (36%)
		OB_NTP_bind	831..917	CDD:400182	19/86 (22%)
		OB-fold-like subdomains. /evidence=ECO:0000250|UniProtKB:Q05B79	841..905		13/71 (18%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250|UniProtKB:Q05B79	849..860		2/10 (20%)
		Necessary for interaction with single-stranded DNA at the 3'-end of the G4-DNA structure. /evidence=ECO:0000250|UniProtKB:Q05B79	870..900		6/29 (21%)
CG3225	NP_608860.1	HrpA	38..>669	CDD:441249	219/750 (29%)