Protein Alignment Kif21a and unc-104

DIOPT Version :10

• Sequence 1: XP_017171930.1
• Gene: Kif21a / 16564
• MGIID: 109188
• Length: 1685
• Species: Mus musculus
• Sequence 2: NP_611155.3
• Gene: unc-104 / 36876
• FlyBaseID: FBgn0267002
• Length: 1739
• Species: Drosophila melanogaster
• Alignment Length: 1756
• Identity: 398/1756 - (22%)
• Similarity: 650/1756 - (37%)
• Gaps: 540/1756 - (30%)

Mouse    23 RIRP----QLAKEK---IEGCHICTSVT-PGEPQVFLGKDKAFTFDYVF------DID-SQQEQI 72
            |:||    ::|:|.   ||.....|::| |..|.......|.|.|||.:      |.| |.|..:
  Fly     9 RVRPFNSREIARESKCIIEMAGATTAITNPKVPPNTSDSVKRFNFDYSYWSHDHHDADFSTQSMV 73

Mouse    73 YTQCIEKLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQ-GIISRAVRHLFKSIDEK 136
            |....|::::..|:|||..:||||||||||:|||     :...||:| |||....:.||..|.:.
  Fly    74 YKDIGEEMLQHSFDGYNVCIFAYGQTGAGKSYTM-----MGRQEEQQEGIIPMICKDLFTRIQDT 133

Mouse   137 KTSAIKNGLPPPEFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTR 201
            :|..:|       :.|...::|:|.|.|.||      ::.|| |.|:|:.|....|.|...::..
  Fly   134 ETDDLK-------YSVEVSYMEIYCERVRDL------LNPKN-KGNLRVREHPLLGPYVEDLSKL 184

Mouse   202 TVNTEPEMMQCLKLGALSRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESS 266
            .|....::...:..|..:||.|:|.||..||||||:|||...|.|....|   |.|..|:
  Fly   185 AVTDYQDIHDLIDEGNKARTVAATNMNETSSRSHAVFTIFFTQRRHDLMT---NLTTEKV----- 241

Mouse   267 PMNEFETLTAKFHFVDLAGSERLKRTGATGERAKEGISINCGLLALGNVISALGD------KSKR 325
                     :|...||||||||...|||.|.|.|||.:||..|..||.|||||.:      .:|:
  Fly   242 ---------SKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEVASKKKNTKK 297

Mouse   326 ATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSPSDRDFMETLNTLKYANRARNIKNKVMVNQDRA 390
            |..:|||||.||.||:::|||||:|.|||.:||:|.::.|||:||:||:||:.|..|.:||:|..
  Fly   298 ADFIPYRDSALTWLLRENLGGNSKTAMIAAISPADINYDETLSTLRYADRAKQIVCKAVVNEDAN 362

Mouse   391 SQQINALRSEITRLQMELMEYKTGKRIIDEEGVE-SINDMFHENAMLQTENNNLRVRIKAMQETV 454
            ::.|..|:.||.:|          :.::..||:| ...|...::.::::...:........:..|
  Fly   363 AKLIRELKEEIQKL----------RDLLKAEGIEVQEEDELTKSTVIKSPTKSRNRNGSTTEMAV 417

Mouse   455 DALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLRAKLLESEAVNENLRKNLTRAT 519
            |.|:|         :.:::|...|..||       .:|..|::|   ::.|||...:      ..
  Fly   418 DQLQA---------SEKLIAELNETWEE-------KLKRTEEIR---VQREAVFAEM------GV 457

Mouse   520 ARSPYFSASSAFSPTILSSDKETIEIIDL-------------AKKDLEKLKRKEKKKKKRLQKLE 571
            |..........|||      |:|..:::|             .|:.|.:|...|....   |.::
  Fly   458 AVKEDGITVGVFSP------KKTPHLVNLNEDPNLSECLLYYIKEGLTRLGTHEANVP---QDIQ 513

Mouse   572 ESG----REERSVAGKDDNADTDQEKKEEKGVSEKENNELDV--------------------EEN 612
            .||    :|..:...|:........|.....|:.::..|.:|                    |:.
  Fly   514 LSGSHILKEHCTFENKNSTVTLLPHKDAIIYVNGRKLVEPEVLKTGSRVILGKNHVFRFTNPEQA 578

Mouse   613 QEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKANYQADLANITCEIAIKQKLIDELENS 677
            :|:.|..:.|.|.|:|.|:.|.:                  |.|.....||:..||. ||.....
  Fly   579 RELRDKIETENEAENEVEKTDTQ------------------QVDWNFAQCELLEKQG-IDLKAEM 624

Mouse   678 QKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSEEKAKKVKCEYEKKLHAMNKEL 742
            :|||..|::||:.:           :|:.||..          ||:.|    .||.::.|:.|::
  Fly   625 KKRLDNLEEQYKRE-----------KLQADQQF----------EEQRK----TYEARIDALQKQV 664

Mouse   743 QRLQTAQKEHARLLKNQSQYEKQLKKLQQDV-------------------MEMKKTKVRLMKQMK 788
            :       |.:..:...|.|..:....::||                   ...:|.:......::
  Fly   665 E-------EQSMTMSMYSSYSPEDFHQEEDVYTNPMYESCWTAREAGLAAWAFRKWRYHQFTSLR 722

Mouse   789 EEQEKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTA-----LRRQVR 848
            ::.....:.....|....:|||   |...|..||              |:.:.:     |...|.
  Fly   723 DDLWGNAIFLKEANAISVELKK---KVQFQFTLL--------------TDTLYSPLPPELASTVA 770

Mouse   849 PMSDK------------VAGKVT----------------------RKLSSSESPAP----DT-GS 874
            |:..:            ||.:||                      |::.:.|||..    || |.
  Fly   771 PVHQEDEFGAPPVSKTLVAVEVTDTKNGATHHWSLEKLRYRLELMRQIYNVESPPSSMLFDTSGM 835

Mouse   875 SAASG--EADTSRPGTQQKMRIPVARVQALPTPTTNG-----------TRKKYQRKGFTGRVFTS 926
            .|.||  ...:..|| ||...:|:..    |..:..|           .|.:..|:.:......|
  Fly   836 EALSGWISPPSQHPG-QQAQLLPLEP----PVESERGRLTLANLIPSRQRLELMREMYHNEAEMS 895

Mouse   927 KTA----------------RMKWQLLERR----VTDI-----IMQKMTISNMEADMNRLLR---- 962
            .|:                |..|..:..|    ::::     ::.|:.|.|...|:...||
  Fly   896 PTSPDYNVESLTGGDPFYDRFPWFRMVGRSFIYLSNLLYPVPLVHKVAIVNERGDVRGYLRIAVQ 960

Mouse   963 ---QREEL-----TKRREKL------SKRREKIVKESGEGDKSVAN-----IIEEMESLTANIDY 1008
               ..|.:     .|:..:|      :|.:.:.:.|..:..:.:.|     .:||:|.:.:....
  Fly   961 PVLDEESIDFNNGVKQSARLVFNEDDAKPKYRALNEKDDVQRYIDNGGLDSKLEELEDVDSGRGI 1025

Mouse  1009 INDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQIK 1073
            .::|.::|.      |.::|.||.|.|.        .|..:.:....:.||  |.:.|....|..
  Fly  1026 DSNSASECH------ENSEEPGEHLQVG--------KEFTFRVTVLQATGI--GAEYADIFCQFN 1074

Mouse  1074 VLEGRLKQTEITSATQNQL------LFHMLK----------EKAELNPELDALLGH--------- 1113
            .|. |.::...|...:|..      .:|:..          |..:..|.:..:.||
  Fly  1075 FLH-RHEEAFSTEPVKNSASGAPLGFYHVQNITVPVTKSFIEYLKTQPIMFKIFGHYQTHPLHKD 1138

Mouse  1114 ALQDLDGAPPE----------NEEDSSEEDGPLHSPGSEGSTL--SSDLM---KLC-----GEVK 1158
            |.||....||.          ::...|.:.|||  |.:..||:  ..|::   ::|     ||..
  Fly  1139 AKQDFVSRPPPRRMLPPSIPISQPVRSPKFGPL--PCAPTSTVLAKHDVLVWFEICELAPNGEYV 1201

Mouse  1159 PK-----------------NKARRR--------TTTQMELLYADSSEV----------ASDTSAG 1188
            |.                 ...:||        .||  |:.:.|.:|:          :||....
  Fly  1202 PSVVEHSDDLPCRGLFLLHQGIQRRIRITIVHEPTT--EVKWKDINELVVGRIRNTPESSDEQDE 1264

Mouse  1189 DAS-LSGPLAPVAEGQEI-GMNTETSGTSARDKELLAPSGLPSKIGS------ISRQSSLSEKKV 1245
            ||. ||..|.| .|..|: |.:.......|.....|..|.|.:::..      |:..:.|..:..
  Fly  1265 DACVLSLGLFP-GEALEVPGDDRSFYRFEAAWDSSLHNSALLNRVSQGGETIYITLSAYLELENC 1328

Mouse  1246 PEPSPVTRRKA---YEKADKPKAKEHKHSDSGASETSLSPPSSPPSRPRNELNVFNRLT------ 1301
            ..|:.:|:..:   |.:..:...:..||..||...       :|.:         ||||
  Fly  1329 ARPAIITKDLSMVIYGRDARTGPRSLKHLFSGQYR-------NPEA---------NRLTGVYELA 1377

Mouse  1302 ----VPQGTPSVQQDKSDESDSSLSEVHSRSTRRGIINPFPACKGVRASPLQCVHIAEGHTKAVL 1362
                ...|:|.||:.:....|:|.:.|.......| ..|    :|
  Fly  1378 LRRASEAGSPGVQRRQRRVLDTSSTYVRGEENLHG-WRP----RG-------------------- 1417

Mouse  1363 CVDSTDDLLFTGS--------KDRTCKVWNLVTGQEIMSLGVHPNNVVSVK--YCNYTSLVFTVS 1417
                 |.|:|...        .:...::.:|:..:|.:.:..:||.....:  .||..:...|..
  Fly  1418 -----DSLIFDHQWELEKLTRLEEVGRMRHLLLLRERLGMDTNPNPTTKTEKDVCNLAARAATSP 1477

Mouse  1418 TSYI------------------KVWDIRES---AKCIRTLTSSGQVTLGEACSASTSRTVAIPSG 1461
            ...:                  :.::.||.   .||::.:  .|:.|..||....|...|: ||.
  Fly  1478 VHMVIPQSPQTPVKDPQQIIPEREYNQREQDLMLKCLKLV--QGRYTKSEANDTQTQSDVS-PSD 1539

Mouse  1462 E 1462
            |
  Fly  1540 E 1540

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Kif21a	XP_017171930.1	KISc_KIF4	23..380	CDD:276823	147/378 (39%)
		Cast	<372..846	CDD:431111	101/535 (19%)
		EnvC	650..>865	CDD:443969	47/272 (17%)
		Rcc_KIF21A	946..1027	CDD:410204	18/103 (17%)
		WD40	1350..1663	CDD:238121	23/144 (16%)
		WD40 repeat	1401..1460	CDD:293791	13/81 (16%)
		WD40 repeat	1466..1501	CDD:293791
		WD40 repeat	1506..1550	CDD:293791
		WD40 repeat	1558..1591	CDD:293791
		WD40 repeat	1598..1634	CDD:293791
unc-104	NP_611155.3	KISc_KIF1A_KIF1B	2..358	CDD:276816	149/384 (39%)
		Kinesin_assoc	355..498	CDD:465047	34/183 (19%)
		FHA_KIF1	474..574	CDD:438757	15/102 (15%)
		YhaN	<575..>666	CDD:443752	33/141 (23%)
		KIF1B	878..924	CDD:463574	6/45 (13%)
		DUF3694	1199..1347	CDD:463599	29/150 (19%)
		PH_KIFIA_KIFIB	1602..1704	CDD:269939