Protein Alignment Itch and Nedd4

DIOPT Version :9

• Sequence 1: NP_001230641.1
• Gene: Itch / 16396
• MGIID: 1202301
• Length: 864
• Species: Mus musculus
• Sequence 2: NP_648993.1
• Gene: Nedd4 / 39958
• FlyBaseID: FBgn0259174
• Length: 1007
• Species: Drosophila melanogaster
• Alignment Length: 1069
• Identity: 344/1069 - (32%)
• Similarity: 468/1069 - (43%)
• Gaps: 336/1069 - (31%)

Mouse     1 MSDSGPQLDSMGSLTMKSQLQITVISAKLKENKKNWFGPS-PYV---------EVTVDGQSKKTE 55
            ::|||   ||       ..|:|.|::.: ...||:.||.| |||         ::.:|....||:
  Fly    63 VNDSG---DS-------CHLRIVVLTGQ-SLAKKDIFGASDPYVRIDLNTINGDINIDSVLTKTK 116

Mouse    56 KCNNTNSPKWKQPLTVIVTPTS-KLCFRVWSHQTLKSDVLLGTAGLDIYETLKSNNMKLEEVVMT 119
            |  .|.:|.|.:.....|.|:. ||.|:|:....|..|..||.                  |.:|
  Fly   117 K--KTLNPTWNEEFIFRVKPSEHKLVFQVFDENRLTRDDFLGM------------------VELT 161

Mouse   120 LQLVGDKEPTETMGDLSVCLDGLQVEAEVVTNGETSCSESTTQNDDGCRTRDDTRVSTNGSEDPE 184
            |..:..::...|:|:.|..|                            |.|
  Fly   162 LVNLPTEQEGRTIGEQSYTL----------------------------RPR-------------- 184

Mouse   185 VAASGENKRANGNNSPSLSNGGFKPSRPPRPSRPPPPTPR-RPASVNGSPSTNSDSDGSSTGSLP 248
             .:.|...|..|                         |.| ..|.:..:...:..|.|:|.|...
  Fly   185 -RSVGAKSRIKG-------------------------TLRIYHAFIRETREQSEPSSGNSDGEWE 223

Mouse   249 PTNTNVNTSTSEGATSGLIIPLTISGGSGPRPLNTVSQAPLPPGWEQRVDQHGRVYYVDHVEKRT 313
            ...     :|:.|.||           :.|.|..|.....||.|||:|.|.:||.|||:|..:.|
  Fly   224 HVE-----ATNAGETS-----------AQPHPFPTGGHDALPAGWEERQDANGRTYYVNHTARTT 272

Mouse   314 TWDRP------------EPLPPGWERR----VD--------------------NMGRIYY----- 337
            .||||            :.|...::||    ||                    |...:.|
  Fly   273 QWDRPTVLNSHSSQSTDDQLASDFQRRFHISVDDTESGRSADSISHNSIEDNNNAAGLAYTPKTA 337

Mouse   338 ---------------------------------VDH----------------------------- 340
                                             |||
  Fly   338 ATSSAPPNTPTNNNGILAQIAMQYRAEEDQDPTVDHTSFVYNSLRHPVAHRQPEISATSLQNDLR 402

Mouse   341 ----------------FTRTTT--------WQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIY 381
                            |||...        ||:.     |..:|.||...|.|...||..|..|.
  Fly   403 PVREAPGVPDIAITNPFTRRAAGNMAGGAGWQQE-----RRRQQMQLHIQQHQQRQQQQQQNRIL 462

Mouse   382 -----------------------GNQDLF-------------ATSQNKEFD-----PL------- 398
                                   .|:|..             :|.:|.|.|     |:
  Fly   463 LDVDHRQQEPQHRGQRHQQQHRPSNEDTDHTDSHNPSDISAPSTRRNSEEDNAAVPPMEQNTGGE 527

Mouse   399 -GPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEK------------PLPEGWEMRFT 450
             .||||.|..:...|||.:|::|.:|.|.|.|||: |:.:..            |||||||.|..
  Fly   528 EEPLPPRWSMQVAPNGRTFFIDHASRRTTWIDPRN-GRASPMPNQTRRVEDDLGPLPEGWEERVH 591

Mouse   451 VDGIPYFVDHNRRATTYIDPRTGKSALDN----GPQIAYVRDFKAKVQYFRFWCQQ-LAMPQHIK 510
            .||..:::|||.|.|.:.|||     |.|    |..:.|.||:|.|.:||:...:: ..:|...:
  Fly   592 TDGRVFYIDHNTRTTQWEDPR-----LSNPNIAGQAVPYSRDYKQKYEYFKSHIRKPTNVPNKFE 651

Mouse   511 ITVTRKTLFEDSFQQIMSFSPQD-LRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFE 574
            |.:.|.::.|||::.|.|.:..| |:.:|||.|.||.||||||:|||||:|||.|:.||.|.|||
  Fly   652 IRIRRTSILEDSYRIISSVTKTDLLKTKLWVEFEGETGLDYGGLAREWFYLLSKEMFNPYYGLFE 716

Mouse   575 YAGKDNYCLQINPASYI-NPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDL 638
            |:..|||.||||..|.: |.:||.||:||||...||::|||.:|..|..||||.:|.||:.|||:
  Fly   717 YSAMDNYTLQINNGSGLCNEEHLSYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLQKPIDLKDM 781

Mouse   639 ESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVA 703
            ||:|.|:||||:|:|||:...  ||:.|.:|:::.|:...|:|||.|.||.||.|||:|||::|.
  Fly   782 ESVDTEYYNSLMWIKENDPRI--LELTFCLDEDVFGQKSQHELKPGGANIDVTNENKDEYIKLVI 844

Mouse   704 EWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYR-HYTRTS 767
            |||....|:||..:|.:||..|:|...::.||..|||:|:||:|.||:.||:.:.:|: .|....
  Fly   845 EWRFVARVKEQMSSFLDGFGSIIPLNLIKIFDEHELELLMCGIQNIDVKDWRENTLYKGDYHMNH 909

Mouse   768 KQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTC 832
            ..|.|||:.|....||.|.|||||||||.|:|:.||.:|.||||||.|.|||.|..|..||:|||
  Fly   910 IIIQWFWRAVLSFSNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQMFTIEKWGTPNNFPRAHTC 974

Mouse   833 FNRLDLPPYKSYEQLKEKLLFAIEETEGF 861
            |||||||||:.|.|||:||:.|||.::||
  Fly   975 FNRLDLPPYEGYLQLKDKLIKAIEGSQGF 1003

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Itch	NP_001230641.1	C2_E3_ubiquitin_ligase	17..140	CDD:175988	34/133 (26%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	151..294		22/143 (15%)
		WW	289..318	CDD:278809	16/28 (57%)
		WW	321..350	CDD:278809	15/143 (10%)
		Required for interaction with FYN. /evidence=ECO:0000250	356..432		32/124 (26%)
		WW	401..430	CDD:278809	12/28 (43%)
		WW	440..471	CDD:197736	16/30 (53%)
		HECTc	509..862	CDD:238033	189/356 (53%)
		HECTc	533..861	CDD:214523	180/330 (55%)
		MAP kinase docking site	535..544		4/8 (50%)
Nedd4	NP_648993.1	C2_NEDD4_NEDD4L	71..204	CDD:175999	43/221 (19%)
		WW	248..277	CDD:278809	16/28 (57%)
		WW	531..560	CDD:278809	12/28 (43%)
		WW	581..613	CDD:197736	18/36 (50%)
		HECTc	650..1003	CDD:238033	187/354 (53%)
		HECTc	674..1003	CDD:214523	180/330 (55%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5021
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000080
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100256
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2273
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	5	4.740