Protein Alignment CYP27A1 and Cyp49a1

DIOPT Version :9

• Sequence 1: NP_000775.1
• Gene: CYP27A1 / 1593
• HGNCID: 2605
• Length: 531
• Species: Homo sapiens
• Sequence 2: NP_001246256.1
• Gene: Cyp49a1 / 36105
• FlyBaseID: FBgn0033524
• Length: 589
• Species: Drosophila melanogaster
• Alignment Length: 544
• Identity: 149/544 - (27%)
• Similarity: 243/544 - (44%)
• Gaps: 82/544 - (15%)

Human    29 AAIPAALPSDKATG------APGAGPGVRRRQRSLEEIPRLGQLRFFFQLFVQGYALQ-----LH 82
            :::||...|..|..      .||..|           :|.:|. .:.|...:..|.:.     ::
  Fly    83 SSLPAETTSSPAAAVRPYSEVPGPYP-----------LPLIGN-SWRFAPLIGTYKISDLDKVMN 135

Human    83 QLQVLY--KAKYGPMWMSYLGPQMHVNLASAPLLEQVMRQEGKYPVRNDMELWKEH-----RDQH 140
            :|.|.|  .||.|.:    :|....:.:.....:..:.::|...|.|..|...:.:     ||..
  Fly   136 ELHVNYGKMAKVGGL----IGHPDLLFVFDGDEIRNIFKKEEAMPHRPSMPSLRHYKGDLRRDFF 196

Human   141 DLTYGPFTTEGHHWYQLRQALNQRLLKPAEAALYTDAFNEVIDDFMTRLDQLRAE--SASGNQVS 203
            ....|.....|..|...||.:...||:|..|..|....|::..:||.|::.:|.|  ....|.:.
  Fly   197 GDVAGLIGVHGPKWEAFRQEVQHILLQPQTAKKYIPPLNDIASEFMGRIELMRDEKDELPANFLH 261

Human   204 DMAQLFYYFALEAICYILFEKRIGCLQRSIPEDTVTFVRSIGLMFQNSLYATFLPKWTRPVL--- 265
            ::    |.:|||::..:..:.|:|||.....|:....:.:|...|           |..|.|
  Fly   262 EL----YKWALESVGRVSLDTRLGCLSPEGSEEAQQIIEAINTFF-----------WAVPELELR 311

Human   266 -PFWKRY-----------LDGWNAIF--SFGKKLIDEKLEDMEAQLQAAGPDGIQVSGYLHFLLA 316
             |.|:.|           ||.:.||.  :.||.:  :|.:..||:..:.....|.:...:.....
  Fly   312 MPLWRIYPTKAYRSFVKALDQFTAICMKNIGKTM--DKADADEARGLSKSEADISIVERIVRKTG 374

Human   317 SGQLSPREAMGSLPELLMAGVDTTSNTLTWALYHLSKDPEIQEALHEEVVGVVPAGQVPQHKD-F 380
            :.:|:...|:    :|.:.||||||...:..:|.|:|:|:.|:.|.:|:..|.|..:...::: .
  Fly   375 NRKLAAILAL----DLFLVGVDTTSVAASSTIYQLAKNPDKQKKLFDELQKVFPHREADINQNVL 435

Human   381 AHMPLLKAVLKETLRLYPVVPTNSRIIEKEIEVDGFLFPKNTQFVFCHYVVSRDPTAFSEPESFQ 445
            ..||.|:|.:|||||:.|||..|.|.::.:..::|:..||.|..:|.|.|||.||..|.||:.|.
  Fly   436 EQMPYLRACVKETLRMRPVVIANGRSLQSDAVINGYHVPKGTHVIFPHLVVSNDPAYFPEPKRFL 500

Human   446 PHRWLRNSQPAT--PRIQ---HPFGSVPFGYGVRACLGRRIAELEMQLLLARLIQKYKVVLAPET 505
            |.|||:.|..|.  |...   |||.|:|||:|.|.|:|||.||:|:..|||::.:||||  :..:
  Fly   501 PERWLKQSTDAAGCPHANQKIHPFVSLPFGFGRRMCVGRRFAEIELHTLLAKIFRKYKV--SYNS 563

Human   506 GELKSVARIVLVPNKKVGLQFLQR 529
            ||.........:|...:..:...|
  Fly   564 GEFVYRVNSTYIPQSPLNFKLTLR 587

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
CYP27A1	NP_000775.1	p450	61..526	CDD:306555	141/501 (28%)
		Sterol-binding. /evidence=ECO:0000255	384..398		8/13 (62%)
Cyp49a1	NP_001246256.1	p450	104..565	CDD:299894	141/499 (28%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	194	1.000	Domainoid score	I3171
eggNOG	1	0.900	-	-		E2759_KOG0159
Hieranoid	0	0.000	Not matched by this tool.
Homologene	1	1.000	-	-		H36040
Inparanoid	1	1.050	201	1.000	Inparanoid score	I3768
Isobase	1	0.950	-	0	Normalized mean entropy	S4770
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D185685at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		mtm8467
orthoMCL	1	0.900	-	-		OOG6_100895
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X156
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	10	9.720