DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Hipk2 and Hipk

DIOPT Version :9

Sequence 1:	NP_034563.2	Gene:	Hipk2 / 15258	MGIID:	1314872	Length:	1196	Species:	Mus musculus
Sequence 2:	NP_001369036.1	Gene:	Hipk / 38070	FlyBaseID:	FBgn0035142	Length:	1409	Species:	Drosophila melanogaster

Alignment Length:	1274	Identity:	487/1274 - (38%)
Similarity:	628/1274 - (49%)	Gaps:	367/1274 - (28%)

- Green bases have known domain annotations that are detailed below.

Mouse    92 HIVVTSASSTSVTGQVLGGPHNLMRRST---VSLLDTYQKCGLKRKSEEIENTSSVQIIEEHPPM 153
            ||:...|:|.| |..|.....:..:|.|   ...:|:|             |:|       |.|.
  Fly    70 HILRQPATSAS-TSSVAAAATSKRKRQTDCDCGCVDSY-------------NSS-------HGPP 113

Mouse   154 IQNNASGA-----------------------TVATATTSTAT----------SKNSGSNSEGDYQ 185
            :|.::|.|                       ::.||.|..||          ||.|.|.::||||
  Fly   114 VQQDSSAAAGAGSVGSKAGSGPGPDGIGPISSLKTAHTKVATSGGHANTQPPSKRSSSGADGDYQ 178

Mouse   186 LVQHEVLCSMTNTYEVLEFLGRGTFGQVVKCWKRGTNEIVAIKILKNHPSYARQGQIEVSILARL 250
            |||||||.|::..|||||||||||||||||||||||:|||||||||||||||||||||||||:||
  Fly   179 LVQHEVLYSLSAEYEVLEFLGRGTFGQVVKCWKRGTSEIVAIKILKNHPSYARQGQIEVSILSRL 243

Mouse   251 STESADDYNFVRAYECFQHKNHTCLVFEMLEQNLYDFLKQNKFSPLPLKYIRPVLQQVATALMKL 315
            |.|:||::|||||:|||||||||||||||||||||||||||||||||||||||:|:||.|||:||
  Fly   244 SQENADEFNFVRAFECFQHKNHTCLVFEMLEQNLYDFLKQNKFSPLPLKYIRPILEQVLTALLKL 308

Mouse   316 KSLGLIHADLKPENIMLVDPSRQPYRVKVIDFGSASHVSKAVCSTYLQSRYYRAPEIILGLPFCE 380
            |.||||||||||||||||||.|||||||||||||||||||.||:|||||||||||||||||||||
  Fly   309 KQLGLIHADLKPENIMLVDPVRQPYRVKVIDFGSASHVSKTVCNTYLQSRYYRAPEIILGLPFCE 373

Mouse   381 AIDMWSLGCVIAELFLGWPLYPGASEYDQIRYISQTQGLPAEYLLSAGTKTTRFFNRDTDSPYPL 445
            ||||||||||:||||||||||||:||:|||||||||||||.|::|::.:||::||.||.||.||.
  Fly   374 AIDMWSLGCVVAELFLGWPLYPGSSEFDQIRYISQTQGLPTEHMLNSASKTSKFFYRDVDSTYPF 438

Mouse   446 WRLKTPDDHEAETGIKSKEARKYIFNCLDDMAQVNMTTDLEGSDMLVEKADRREFIDLLKKMLTI 510
            |||||.::|||||..|||||||||||||||:.|||:.|||||..:|.||.||||||||||:||||
  Fly   439 WRLKTTEEHEAETNTKSKEARKYIFNCLDDIGQVNVPTDLEGGQLLAEKTDRREFIDLLKRMLTI 503

Mouse   511 DADKRVTPIETLNHPFVTMTHLLDFPHSAHVKSCFQNMEICKRRVNMYDTVNQSKTPFITHVAPS 575
            |.::|:||.|.|||.|..:|||:|:.:..:||:..|.||:|:|  ..:.||..:.| .:|:..||
  Fly   504 DQERRLTPAEALNHSFTRLTHLVDYVYCNNVKASVQMMEVCRR--GDFHTVQPAST-LVTNFVPS 565

Mouse   576 TSTNLTMTFNNQLTT-----VHNQAPTTSSATLSLANPEVSILNYQSALYQ-PSAASMAAVAPRS 634
            ::.|:|.|.|||||:     |.:..|                |.|: .||| .:..|:|...|::
  Fly   566 STENMTFTINNQLTSQVQRLVRDGRP----------------LAYE-GLYQIYNGRSVARQYPQT 613

Mouse   635 MPLQTGTAQICARPDPFQQAL---IVCPPGFQGLQASPSKHAGYSVRMENAVPIVTQAPGAQPLQ 696
                        |.|.||..|   |:|||.:|.: .||:||.           :|..|....|||
  Fly   614 ------------RTDSFQHQLVSNILCPPSYQTM-PSPTKHV-----------VVGSATMQPPLQ 654

Mouse   697 IQPG--------------------LLAQQ------AWPGGAQQILLPPAWQQLTGVATHTSVQHA 735
            :.|.                    ||..:      |||...:|:.|.|:|.|       .:..|:
  Fly   655 VPPQQYVNVPVPVSMVEPTSGQRMLLTNRVQASGVAWPQTGRQMALVPSWPQ-------QAPAHS 712

Mouse   736 AVIPET-MAGTQQLADWRNTHAHGSHYNPIMQQPALLTGHVTLPAAQPLNVGVAHVMRQQPTSTT 799
            .::..| :...:::        :..|             |:.||.........||.:.:..:...
  Fly   713 LIVDSTPLFNVEEI--------YPKH-------------HLNLPRNDLKKESPAHHIAKGNSYRV 756

Mouse   800 SSRKSKQHQSSVRNVSTCEVTSSQAISSPQRSKRVKENTPP------RCAMVHSSPACSTSVTCG 858
            ...:.|:||.                .||.: |||||::||      |.|  |.||.......|.
  Fly   757 PRHEKKEHQQ----------------LSPVK-KRVKESSPPHQQRYQRAA--HVSPQYHAHHNCN 802

Mouse   859 WGDVASSTTRERQRQTIVIPDTPSPTVSVITISSDTDEEEEQKHAPTSTVSKQRKNVISCVTVHD 923
            :|:.:..:.....    |:..:.:.:.:::..||.:....   |.|.:               |.
  Fly   803 YGNGSGYSASAGS----VVASSAASSSNIVNGSSSSSHHH---HVPVA---------------HA 845

Mouse   924 SPYSDSSSNTSPYSVQQRTGHNGTNTLDTKGG--------------------------------- 955
            .|||.||            ||:..:..:..||
  Fly   846 QPYSSSS------------GHHIVSNCNANGGAGGAVVYQQPPAHAHQQHGQQPHPQSSQHPQHV 898

Mouse   956 ----LENHCTGNPRTII----------------VPPLKTQASEVLVECDSLGPAISASHHSSSFK 1000
                :..|.|.:|..:|                ||.||.:|                  |:.|
  Fly   899 KQPTITIHDTPSPTAVITISDSEDEGGEAGGAQVPVLKQRA------------------HAQS-- 943

Mouse  1001 SKSSSTVTSTSG----HS-SGSSSGAIAYRQQRPGPHFQQQQPLNL-------SQAQQH-MAADR 1052
                    .|||    |: |.|.|.:.|::|..|.|..||||.:|.       ::.:.| .||..
  Fly   944 --------QTSGSLLQHAPSSSCSRSSAHQQPHPHPQQQQQQQINYGDHDPEDARRRHHAAAAAA 1000

Mouse  1053 TGS-----HRRQQAYITPTMAQAPYTFPHNSPSHGTVHPH---------------LAAA------ 1091
            .||     |::||....|...|.....|...|......|.               ||.|
  Fly  1001 VGSPKHHHHQQQQQPAPPPQQQQQQYQPQPPPQQVLPQPQPSIKYEPGQSQKKRILAMAQSECGY 1065

Mouse  1092 -AHLPTQPHLYTYTAPTALGSTGTVAHLVASQGSARHTVQHTAYPASIVHQ-----VPVSMGPRV 1150
             ..:|:||           .|:.::.|:...|..|....::.|.|...:..     .|.|.|..|
  Fly  1066 QPQVPSQP-----------SSSASLPHIPTKQEPAEFYPEYAAAPPQQLDTKRSSWAPTSSGSGV 1119

Mouse  1151 LPSPTIHPSQYPAQFAHQTYISASPASTVYTGYPLSPAK 1189
            ...|..||.:.....|..:|::.|.|.      ||:.:|
  Fly  1120 SALPLAHPKREAPSVAPISYVAPSVAP------PLAHSK 1152

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Hipk2	NP_034563.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	50..69
		Transcriptional corepression	97..230		66/168 (39%)
		STKc_HIPK2	183..537	CDD:271129	290/353 (82%)
		Interaction with DAXX. /evidence=ECO:0000250	189..520		275/330 (83%)
		S_TKc	199..527	CDD:214567	274/327 (84%)
		Interaction with SKI and SMAD1. /evidence=ECO:0000250	539..844		82/346 (24%)
		Interaction with POU4F1. /evidence=ECO:0000269\|PubMed:15492043	752..897		28/150 (19%)
		Interaction with CTBP1. /evidence=ECO:0000269\|PubMed:14567915	774..876		24/107 (22%)
		Interaction with HMGA1. /evidence=ECO:0000269\|PubMed:11593421	787..897		24/115 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	792..847		14/60 (23%)
		Nuclear localization signal 1 (NLS1). /evidence=ECO:0000250	802..805		0/2 (0%)
		Nuclear localization signal 2 (NLS2). /evidence=ECO:0000250	832..835		2/2 (100%)
		Interaction with TP53 and TP73. /evidence=ECO:0000269\|PubMed:11780126	839..934		20/100 (20%)
		Localization to nuclear speckles	873..980		24/159 (15%)
		Required for localization to nuclear speckles. /evidence=ECO:0000250	873..980		24/159 (15%)
		Interaction with UBE2I. /evidence=ECO:0000269\|PubMed:10535925	873..907		5/33 (15%)
		SUMO interaction motifs (SIM), required for nuclear localization and kinase activity. /evidence=ECO:0000250	884..908		4/23 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	891..963		16/108 (15%)
		Interaction with AXIN1. /evidence=ECO:0000269\|PubMed:15526030	935..1050		32/180 (18%)
		Autoinhibitory domain (AID). /evidence=ECO:0000250	984..1196		57/251 (23%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	991..1058		24/84 (29%)
Hipk	NP_001369036.1	STKc_HIPK	192..520	CDD:271113	274/327 (84%)
Hipk	NP_001369036.1	PHA03378	<1080..1327	CDD:223065	19/79 (24%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	582	1.000	Domainoid score	I212
eggNOG	1	0.900	-	-		E1_KOG0667
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	693	1.000	Inparanoid score	I681
Isobase	1	0.950	-	0	Normalized mean entropy	S2156
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D38932at33208
OrthoFinder	1	1.000	-	-		FOG0002956
OrthoInspector	1	1.000	-	-		otm42611
orthoMCL	1	0.900	-	-		OOG6_103159
Panther	1	1.100	-	-	LDO	PTHR24058
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R968
SonicParanoid	1	1.000	-	-		X805
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	15	14.810