DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Hspa5 and Hsp70Bb

DIOPT Version :9

Sequence 1:	NP_001156906.1	Gene:	Hspa5 / 14828	MGIID:	95835	Length:	655	Species:	Mus musculus
Sequence 2:	NP_524927.2	Gene:	Hsp70Bb / 48582	FlyBaseID:	FBgn0013278	Length:	641	Species:	Drosophila melanogaster

Alignment Length:	619	Identity:	380/619 - (61%)
Similarity:	486/619 - (78%)	Gaps:	13/619 - (2%)

- Green bases have known domain annotations that are detailed below.

Mouse    32 VGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDA 96
            :|||||||||||||:::|:|||||||||||.|||||||| :.||||||.||||:..||.||||||
  Fly     4 IGIDLGTTYSCVGVYQHGKVEIIANDQGNRTTPSYVAFT-DSERLIGDPAKNQVAMNPRNTVFDA 67

Mouse    97 KRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAY 161
            ||||||.::||.:.:|:|..|||||....||.|.|:. .|::|.|||||||:|||||||||||||
  Fly    68 KRLIGRKYDDPKIAEDMKHWPFKVVSDGGKPKIGVEY-KGESKRFAPEEISSMVLTKMKETAEAY 131

Mouse   162 LGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKR-EGEKNILVFD 225
            ||:.:|.||:||||||||:|||||||||.||||||:||||||||||:||||||. :||:|:|:||
  Fly   132 LGESITDAVITVPAYFNDSQRQATKDAGHIAGLNVLRIINEPTAAALAYGLDKNLKGERNVLIFD 196

Mouse   226 LGGGTFDVSLLTIDNG-VFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKL 289
            |||||||||:||||.| :|||.:|.|||||||||||.|::.|..:.:|:|..||:|.:.||:::|
  Fly   197 LGGGTFDVSILTIDEGSLFEVRSTAGDTHLGGEDFDNRLVTHLAEEFKRKYKKDLRSNPRALRRL 261

Mouse   290 RREVEKAKRALSSQHQARIEIESFFEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKK 354
            |...|:|||.|||..:|.|||::.|||:||...::||:||||..||||:|::||:|.|.|:.:.|
  Fly   262 RTAAERAKRTLSSSTEATIEIDALFEGQDFYTKVSRARFEELCADLFRNTLQPVEKALNDAKMDK 326

Mouse   355 SDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQD--TGDLVL 417
            ..|.:||||||||||||:|.|::|||:||..:..|||||||||||||||.:|||||.  ..|::|
  Fly   327 GQIHDIVLVGGSTRIPKVQSLLQEFFHGKNLNLSINPDEAVAYGAAVQAAILSGDQSGKIQDVLL 391

Mouse   418 LDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGT 482
            :||.||:|||||.||||||||.||..:|.|:::.|||.|||||.|:|:||||||.:||||:.|||
  Fly   392 VDVAPLSLGIETAGGVMTKLIERNCRIPCKQTKTFSTYSDNQPGVSIQVYEGERAMTKDNNALGT 456

Mouse   483 FDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAE 547
            |||:||||||||||||||||::|.||||.|:|::..||....|||.||:.||:..||:||||:||
  Fly   457 FDLSGIPPAPRGVPQIEVTFDLDANGILNVSAKEMSTGKAKNITIKNDKGRLSQAEIDRMVNEAE 521

Mouse   548 KFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDA 612
            |:|:||:|.::||.:||.||||.:::|..:  ::...|||...||.::.....|.|.||:|:..|
  Fly   522 KYADEDEKHRQRITSRNALESYVFNVKQSV--EQAPAGKLDEADKNSVLDKCNETIRWLDSNTTA 584

Mouse   613 DIEDFKAKKKELEEIVQPIISKLY-----GSGGP 641
            :.|:|..|.:||.....||::|::     .:|||
  Fly   585 EKEEFDHKMEELTRHCSPIMTKMHQQGAGAAGGP 618

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Hspa5	NP_001156906.1	Required for interaction with KIAA1324. /evidence=ECO:0000250\|UniProtKB:P11021	1..81		39/48 (81%)
		HSP70	31..636	CDD:365808	377/607 (62%)
		Nucleotide-binding (NBD). /evidence=ECO:0000250\|UniProtKB:P11021	126..281		111/156 (71%)
		Interdomain linker. /evidence=ECO:0000250\|UniProtKB:G3I8R9	410..420		3/11 (27%)
		Substrate-binding (SBD). /evidence=ECO:0000250\|UniProtKB:P11021	421..501		58/79 (73%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	632..655		4/15 (27%)
		Prevents secretion from ER	652..655
Hsp70Bb	NP_524927.2	PTZ00009	2..641	CDD:240227	380/619 (61%)
Hsp70Bb	NP_524927.2	HSPA1-2_6-8-like_NBD	3..379	CDD:212675	250/376 (66%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0443
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG53894
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000049
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100083
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	5	4.720