DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment E4f1 and Kr-h1

DIOPT Version :9

Sequence 1:	NP_001288713.1	Gene:	E4f1 / 13560	MGIID:	109530	Length:	783	Species:	Mus musculus
Sequence 2:	NP_477466.1	Gene:	Kr-h1 / 33861	FlyBaseID:	FBgn0266450	Length:	845	Species:	Drosophila melanogaster

Alignment Length:	642	Identity:	147/642 - (22%)
Similarity:	225/642 - (35%)	Gaps:	176/642 - (27%)

- Green bases have known domain annotations that are detailed below.

Mouse   275 TEKIRFSISKDTAVGKEEVPAGSSAST-----------VGTVTSSVAGDPMETSPVIHLVTDAKG 328
            ||.:..|.|...||..::.||..||:|           :.|..||.|...::..|.:...|.:.|
  Fly    27 TELLDISKSPAKAVAVKKSPAKDSATTKMVYYSANQLLIKTEQSSQAQFCLQVPPPLTATTTSVG 91

Mouse   329 TVIHEVHVQMQELPLGMKALTPESPDSE-ELPCSSENSRENLL--------HQAMQNSGIVLERV 384
            ..:.....|.:...|   ..||:....: :|....:..::..:        ||..|..
  Fly    92 LGVPPSGGQQEHFEL---LQTPQQRQMQLQLQDQHQQEQQQFVSYQLAIQQHQKQQQQ------- 146

Mouse   385 AGEESALEPAPPSGSSPQCLGDGSPELPLLKVE---------QIETQVASEAATVPRTHPCPQCS 440
            ...||....||.:..|.|          .:|.|         .:.:||...:|:.|: ..|.||.
  Fly   147 QQHESITNAAPTAAPSAQ----------RIKTEPVGGFPASAAVVSQVRKPSASKPQ-FKCDQCG 200

Mouse   441 ETFPTAATLEAHKRGH-----------------------------------------IAP----- 459
            .||.:.:...:|.:.|                                         |.|
  Fly   201 MTFGSKSAHTSHTKSHSKNQDLSLNGASGAGVAAPVSTAAIELNDAGLPVGIPKSPTIKPLANVA 265

Mouse   460 ---RPFTCTQCGKAFPKAYLLKKHQEVHVHERRFRCGDCGKLYKTIAHVRGHRRVHSDERPFPCP 521
               .|:.|..|.|.|.....|.:|...|..||.|.|..|.||:....:::.|||:|:.|||:.|.
  Fly   266 AGADPYQCNVCQKTFAVPARLIRHYRTHTGERPFECEFCHKLFSVKENLQVHRRIHTKERPYKCD 330

Mouse   522 QCGKRYKTKNAQQVHFRTHLEEKPHVCQFCSRGFREKGSLVRHVRHHTGEKPFKCYK--CGRGFA 584
            .||:.::.......|.|.|..|:||.|..|.:.|.:.|.||.|:|.||||||:||.:  ||:||.
  Fly   331 VCGRAFEHSGKLHRHMRIHTGERPHKCSVCEKTFIQSGQLVIHMRTHTGEKPYKCPEPGCGKGFT 395

Mouse   585 EHGTLNRHLRTKGGCLLEVEELLVSEESPSAAATVLAEDPHTVLVEFSSVVADTQEY-------- 641
            ....|..|.||..|            |.|........:..:..:::...|    |.|
  Fly   396 CSKQLKVHSRTHTG------------EKPYHCDICFRDFGYNHVLKLHRV----QHYGSKCYKCT 444

Mouse   642 IIEATADDTETSEATEIIEGTQTEV-DSHIMKVVQQIVHQAGAGHQI---IVQNVTMDQETALGS 702
            |.:.|..:.:..||.  |:|...|| |...............||...   .:|.|:.:.|::..|
  Fly   445 ICDETFKNKKEMEAH--IKGHANEVPDDEAEAAAASAAASTSAGSSAGSPSLQGVSSNSESSNHS 507

Mouse   703 -------------------EATAADTITIAT-----PESLTEQVAMTLASAIS------------ 731
                               ..|.|.|::|.|     |.||:...:.:.:|..|
  Fly   508 PPSSPPATKKPRQARQPRVSKTVAATLSIPTSSPLSPSSLSSTYSPSASSMASPPPTSAHYLPVQ 572

Mouse   732 -EGTVLTARAGPNSTEQATVTMVSSE--DIEILEHGGEL------VIASPEGQLEVQ 779
             |...|:..:|.:|.:.|..|....|  |:.:.:..|:|      ...:|...||:|
  Fly   573 MEADALSRDSGVSSAQPAHSTYADEEPTDLSMQQVQGQLPESTVDYYQAPPSLLELQ 629

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
E4f1	NP_001288713.1	Required for ubiquitin ligase activity. /evidence=ECO:0000250	40..84
		Mediates dimerization, DNA-binding, transcription repression of CCNA2 and interaction with HMGA2. /evidence=ECO:0000250	185..264
		COG5048	193..594	CDD:227381	100/398 (25%)
		C2H2 Zn finger	195..215	CDD:275368
		zf-C2H2	221..243	CDD:278523
		C2H2 Zn finger	223..243	CDD:275368
		zf-H2C2_2	235..258	CDD:290200
		C2H2 Zn finger	251..269	CDD:275368
		Mediates interaction with CDKN2A. /evidence=ECO:0000250	368..565		61/262 (23%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	386..407		6/20 (30%)
		Interaction with BMI1. /evidence=ECO:0000250	434..598		64/214 (30%)
		C2H2 Zn finger	436..456	CDD:275368	6/19 (32%)
		zf-C2H2	462..484	CDD:278523	6/21 (29%)
		C2H2 Zn finger	464..484	CDD:275368	6/19 (32%)
		C2H2 Zn finger	492..512	CDD:275368	7/19 (37%)
		zf-H2C2_2	504..529	CDD:290200	10/24 (42%)
		Mediates interaction with TP53. /evidence=ECO:0000250	520..579		25/60 (42%)
		C2H2 Zn finger	520..540	CDD:275368	5/19 (26%)
		zf-H2C2_2	534..555	CDD:290200	8/20 (40%)
		C2H2 Zn finger	548..568	CDD:275368	8/19 (42%)
		zf-H2C2_2	560..584	CDD:290200	15/25 (60%)
		Mediates interaction with RASSF1. /evidence=ECO:0000250	574..596		9/23 (39%)
		C2H2 Zn finger	576..595	CDD:275368	7/20 (35%)
Kr-h1	NP_477466.1	C2H2 Zn finger	196..216	CDD:275368	6/19 (32%)
		COG5048	<270..420	CDD:227381	58/161 (36%)
		zf-C2H2	271..293	CDD:278523	6/21 (29%)
		C2H2 Zn finger	273..293	CDD:275368	6/19 (32%)
		zf-H2C2_2	286..309	CDD:290200	10/22 (45%)
		C2H2 Zn finger	301..321	CDD:275368	7/19 (37%)
		zf-H2C2_2	313..338	CDD:290200	10/24 (42%)
		C2H2 Zn finger	329..349	CDD:275368	5/19 (26%)
		zf-H2C2_2	344..366	CDD:290200	9/21 (43%)
		C2H2 Zn finger	357..377	CDD:275368	8/19 (42%)
		zf-H2C2_2	370..396	CDD:290200	16/25 (64%)
		C2H2 Zn finger	385..407	CDD:275368	8/21 (38%)
		zf-H2C2_2	400..424	CDD:290200	7/35 (20%)
		C2H2 Zn finger	415..435	CDD:275368	1/23 (4%)
		zf-C2H2	441..463	CDD:278523	5/23 (22%)
		C2H2 Zn finger	443..463	CDD:275368	5/21 (24%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR24390
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	1.100