Protein Alignment STXBP5 and Tomosyn

DIOPT Version :10

• Sequence 1: XP_047274153.1
• Gene: STXBP5 / 134957
• HGNCID: 19665
• Length: 1179
• Species: Homo sapiens
• Sequence 2: NP_727628.3
• Gene: Tomosyn / 32217
• FlyBaseID: FBgn0030412
• Length: 1470
• Species: Drosophila melanogaster
• Alignment Length: 1507
• Identity: 549/1507 - (36%)
• Similarity: 777/1507 - (51%)
• Gaps: 367/1507 - (24%)

Human     1 MRKFNIRKVLDGLTAGSSSASQQQQQQHPPGNR-EPEIQETLQSEHFQLCKTVRHGFPYQPSALA 64
            |:||..:.||||.          :|...|...| |.||||.|:::||.|.||.||||||.|::.|
  Fly     1 MKKFTFKGVLDGF----------RQSVQPQATRQEQEIQEQLKADHFTLKKTFRHGFPYSPTSFA 55

Human    65 FDPVQKILAVGTQTGALRLFGRPGVECYCQH--DSGAAVIQLQFLINEGALVSALADDTLHLWNL 127
            ||||||:||:|.::|.:|:.|||||:.:.:|  :|..||:..|||:||||||:..||||:|||::
  Fly    56 FDPVQKLLAIGDKSGYIRILGRPGVDAHAKHEGESECAVLFAQFLVNEGALVTVTADDTIHLWSI 120

Human   128 RQKRPAILHSLKFCRERVTFCHLPFQSKWLYVGTERGNIHIVNVESFTLSGYVIMWNKAIELSSK 192
            |||.|.|:.||||.|||||..|||..|||||||||:||||:|::::|.||||:|.||||||:...
  Fly   121 RQKTPRIVQSLKFQRERVTCIHLPVGSKWLYVGTEKGNIHVVHIDTFALSGYIINWNKAIEVVRT 185

Human   193 SHPGPVVHISDNPMDEGKLLIGFESGTVVLWDLKSKKADYRYTYDEAIHSVAWHHEGKQFICSHS 257
            ||||.|:.:.|||:|..||||.||.|.:||||||:|.|:.|:...||:.|:|||:|||.|:.||:
  Fly   186 SHPGAVIALCDNPLDANKLLIAFECGLLVLWDLKAKCAELRWQAAEAVKSLAWHYEGKYFVSSHT 250

Human   258 DGTLTIWNVRSPAKPVQTITPHGKQLKDGKKPEPCKPILKVEFKTTRSGEPFIILSGGLSYDTVG 322
            ||::..|..:..|||...:.||.|..||| ..|.||||.||:.|::.:||.|.|.|||:..:...
  Fly   251 DGSICSWPTKPQAKPQSQVCPHAKINKDG-NAEKCKPIYKVDLKSSATGETFTIFSGGMPSEKGS 314

Human   323 RRPCLTVMHGKSTAVLEMDYSIVDFLTLCETPYPNDFQEPYAVVVLLEKDLVLIDLAQNGYPIFE 387
            :..|:|||.||:|.||||::::.||:||||.|:|.:.|||||:.|||:.|||||||...|:|.||
  Fly   315 KSNCITVMVGKATTVLEMEHAVCDFITLCENPWPCETQEPYAIAVLLQYDLVLIDLLTPGFPCFE 379

Human   388 NPYPLSIHESPVTCCEYFADCPVDLIPALYSVG--ARQKRQGYSKKEWPINGGNWGLGAQSYPEI 450
            :|||:.:||||||||.|..|||.||:||.||||  ...|:..:|::||||:||.|...:.||.||
  Fly   380 SPYPMDLHESPVTCCTYLTDCPSDLVPAFYSVGRTTTSKKSCFSEREWPISGGEWSPASCSYSEI 444

Human   451 IITGHADGSVKFWDASAITLQVLYKLKTSKVFEKSRN--KDDRPNTDIVDEDPYAIQIISWCPES 513
            :||||.|||:||||:.|.|||:||||||:|:|||.|:  ....|.:|    :|.|:.:|..|.||
  Fly   445 VITGHQDGSLKFWDSGAGTLQILYKLKTAKMFEKPRSHVAHSHPESD----NPLAVHLIFLCSES 505

Human   514 RMLCIAGVSAHVIIYRFSKQEVITEVIPMLEVRLLYEINDVETPEGEQPPPLPTPVGGSNPQPIP 578
            |.||:||....|::::|.|.|..:||: :||:.:|||..|              .:.|::|:...
  Fly   506 RRLCVAGAMGQVMLFKFRKVESTSEVL-VLEIPILYENFD--------------DIYGTSPECDF 555

Human   579 PQSHPSTSSSSSDGLRDNVPCLKVKNSPLKQSPGYQTELVIQLVWVGGEPPQQITSLAVNSSYGL 643
            ...|....:.|||..:.....|||:|...::.||:|::||. |.........|:|||.::|||||
  Fly   556 LTGHQVQKAESSDSDKTAECSLKVRNGAQRKPPGFQSQLVC-LTTGPNRRNVQVTSLCISSSYGL 619

Human   644 VVFGNCNGIAMVDYLQKAVLLNLGTIELYGSNDPYRREPRSPRK-------SRQPSGGVLI---- 697
            :.:|...|:.::|.:||..||::...:|||::|||.|.|:||::       ||.||...|.
  Fly   620 MAYGTEYGLVIIDIIQKICLLSVACPDLYGAHDPYSRTPKSPKRIENKEEQSRSPSSDQLNDTTS 684

Human   698 -------------VTSCMCGLSSLYSES-------MKKLRSSFLTGLCDISEGTVVPED--RCKS 740
                         .||...|:::..|..       :...|.:.|:|....:........  ..||
  Fly   685 PDSPSLEFVPEAGETSAHDGVATATSSRPSSPDPVVNSARETLLSGSAAATAAAAASAAVMMAKS 749

Human   741 PTSGSSSPHNSDDEQK------------------------MNNFIEKVKTKS------------- 768
            |....::|....|.:|                        :|..:|.:|..|
  Fly   750 PQRDDAAPKELQDRRKSMSWKTFNLKRQLSKVNMKIGGLNVNTDMESLKNSSIFYTPKEVSPEAA 814

Human   769 -----------------------------------------RKF------------SKMVANDIA 780
                                                     .||            |..:|:.|.
  Fly   815 TPPATEEEATPEGKEDESLDDGEMATNAGAAAAASPSPAVRGKFRSSTTDSPDEGASSSIASGIR 879

Human   781 KM--------------------------------------SRKLSLPT-------------DLKP 794
            ::                                      .|.||:|.             :||.
  Fly   880 RVDFEQPAGVEVERPNNLPLADAPAPLKPARQKFKEKSRDQRLLSVPNIKYQPRDSRGGARNLKN 944

Human   795 D------------------------------LDVKDNSFSRSRSSSVTSIDKESREAISALHFCE 829
            |                              :...|.:||||||||::|||..|.|:::.|.|.|
  Fly   945 DVSPLMGGGGGGGGVVGASSGIGGSVAGGKKIHKLDGTFSRSRSSSMSSIDMSSSESVTCLAFIE 1009

Human   830 TFTRKTD-SSPSPCLWVGTTLGTVLVIALNLPPGGEQRLLQPVIVS-PSGTILRLKGAILRMAFL 892
            ::.::.| .:..|.||:||:.|::|.:.:.:|. .:.|..|||::: ..|.::||||:|..|:||
  Fly  1010 SYAKRNDILTLVPTLWIGTSFGSILTLFITMPE-RDVRKSQPVLITINGGPVVRLKGSITSMSFL 1073

Human   893 DTTGCLIPPAYEPWREHNVPEEKDEKEKLKKRRPVSVSP-------------------------- 931
            |:.|.:||..:|.||:    |::|.|::...:.....||
  Fly  1074 DSYGSIIPYTFETWRD----EKRDNKDRTPTKSSSRTSPTFTPTTANTLSNTSVAGGASAGGGAG 1134

Human   932 ---------------------------------------------------SSS----------Q 935
                                                               |||          :
  Fly  1135 GGGGAGGGAASGGAAAGGPAGGGAGAGSAAGAAGGVGSGVTSGGGMSSSSASSSGISGSVSTGLE 1199

Human   936 EISENQYAVICSEKQAKVISLPTQNCAYKQNITETSFVLRGDIVALSNSICLACFCANGHIMTFS 1000
            .:::.||.||.||||.||..:..|.|..:..::|..|.::.:.:.:.:..|||.:.:|||:|..|
  Fly  1200 TLTDRQYIVIASEKQTKVFDVANQCCINRIQLSEMDFAVKAETITMKDGSCLATYLSNGHLMVHS 1264

Human  1001 LPSLRPLLDVYYLPLTNM-----------------------------RIARTFCFTNNGQALYLV 1036
            ||||:.|||..:|||..:                             :|::.|||::.|..||:.
  Fly  1265 LPSLKLLLDTDFLPLMELSFQTKCKQGIVDPMLSIWGQQIIVHEDTTQISKIFCFSHKGHGLYMA 1329

Human  1037 SPTEIQRLTYSQETCENLQEMLGELFTPVETPEAPNRGFFKGLFGGGAQSLDREELFGESSSGKA 1101
            ||||||:.|.|.|.|:.:.||:|||:|..|.||.|..|||||||||||:.||||||||| .|||.
  Fly  1330 SPTEIQKFTISSEFCQFILEMMGELYTVHEMPEQPKEGFFKGLFGGGAKLLDREELFGE-QSGKP 1393

Human  1102 SRSLAQHIPGPGGIEGVKGAASGVVGELARARLALDERGQKLGDLEERTAAMLSSAESFSKHAHE 1166
            :||:|:||||| .:|.:...||....|::||.....|||:||..||||...|.::|:.||..||:
  Fly  1394 NRSVARHIPGP-NLEQLGQRASTAASEISRAHQLAMERGEKLNLLEERAERMANTAQDFSGTAHQ 1457

Human  1167 IMLKYKDKKWYQ 1178
            :|||||||||||
  Fly  1458 LMLKYKDKKWYQ 1469

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
STXBP5	XP_047274153.1	WD40	<48..280	CDD:441893	134/233 (58%)
		WD40 repeat	61..94	CDD:293791	17/32 (53%)
		WD40 repeat	102..140	CDD:293791	23/37 (62%)
		WD40 repeat	145..191	CDD:293791	31/45 (69%)
		WD40 repeat	199..235	CDD:293791	20/35 (57%)
		WD40 repeat	240..277	CDD:293791	16/36 (44%)
		LLGL	275..384	CDD:462446	56/108 (52%)
		R-SNARE_STXBP5	1112..1172	CDD:277246	25/59 (42%)
Tomosyn	NP_727628.3	WD40	<41..257	CDD:441893	128/215 (60%)
		WD40 repeat	95..133	CDD:293791	23/37 (62%)
		WD40 repeat	138..184	CDD:293791	31/45 (69%)
		WD40 repeat	192..226	CDD:293791	19/33 (58%)
		LLGL	268..376	CDD:462446	56/108 (52%)
		R-SNARE_STXBP5_6	1403..1463	CDD:277226	26/60 (43%)