DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment COL4A4 and vkg

DIOPT Version :9

Sequence 1:	NP_000083.3	Gene:	COL4A4 / 1286	HGNCID:	2206	Length:	1690	Species:	Homo sapiens
Sequence 2:	NP_001260071.1	Gene:	vkg / 33726	FlyBaseID:	FBgn0016075	Length:	1940	Species:	Drosophila melanogaster

Alignment Length:	1837	Identity:	748/1837 - (40%)
Similarity:	879/1837 - (47%)	Gaps:	294/1837 - (16%)

- Green bases have known domain annotations that are detailed below.

Human    30 LFSVQYVYGSGKKYIGPCGGRDC--SVCHCVPEKGSRGPPGP---PGPQGPIGPLGAPGPIGLSG 89
            |..|.|:.|| ...:....|:.|  ::|.|...||..|.|||   ||.:||.|.:|.||..|..|
  Fly    12 LLGVVYLLGS-LVSVTLADGKICNTTLCDCKGIKGRMGAPGPIGVPGLEGPAGDIGPPGRAGPLG 75

Human    90 EKGMRGDRGPPGAAGDKGDKGPTGVPGFPGLDGIPGHPGPPGPRGKPGMSGHNGSRGDPGFPGGR 154
            |||..|:.|..|..|.:||.||.|..|:||:.|..|.||.|||||..|..|..|.:|..|.||..
  Fly    76 EKGDVGEYGEQGEKGHRGDIGPKGEMGYPGIMGKSGEPGTPGPRGIDGCDGRPGMQGPSGAPGQN 140

Human   155 GALGPGGPLGH---PGEKGEKG-NSVFILGAVKGIQGDRGD---------PGLPGLPGSWGAGGP 206
            |..||.|..|.   |||.||.| ||       ||.:|:||:         ||..|..||.|..|.
  Fly   141 GVRGPPGKPGQQGPPGEAGEGGINS-------KGTKGNRGETGQPGGVGPPGFDGDRGSKGDTGY 198

Human   207 AGPTGYPGEPGLVGPPGQPGR-----------PGLKGNPGVGVKGQM-------GDPGEVGQQGS 253
            ||.||..|:|||.||.|..|.           ||.||.||..:.|.:       |..|.||.||.
  Fly   199 AGLTGEKGDPGLPGPKGDTGAVSELPYSLIGPPGAKGEPGDSLSGVLKPDDTLKGYKGYVGLQGD 263

Human   254 PGPTLLVEPPDFCLYKGEKGIKGIPGMVGLPGPPGRKGESGIGAKGEKGIP---GFPGPRGDPGS 315
            .||               :|..|..|.||..|.||.:||  ||..||:|.|   |.||..||.|.
  Fly   264 EGP---------------QGPTGEQGAVGRNGLPGARGE--IGGPGERGKPGKDGEPGRFGDKGM 311

Human   316 YGSPGF---------PGLKGELGLVGDPGLFGLIGPKG--DP----------GNRGHPGPPGVLV 359
            .|:||:         ||.:||.|..|.||:.|..||.|  ||          |::|..||||.
  Fly   312 KGAPGWTGADGLDGSPGERGEDGFTGMPGVQGGAGPPGIYDPSLTKSLPGPIGSQGDIGPPGE-- 374

Human   360 TPPLPLKGPPGDPGFPGRYGETGDVGPPGPPGLLG--------RPGEA-CAGMIGPPGPQGFPGL 415
                  :||||.||.|||.|..|..|..|.|||.|        ..||| ..|.||||||||.||.
  Fly   375 ------QGPPGLPGKPGRRGPIGLAGQSGDPGLNGSRGPPGRSERGEAGDYGFIGPPGPQGPPGE 433

Human   416 PGLPGEAGIPGRPD----SAPGKPGKPGSPGLP---------GAPGLQGLPGSSVIYCSVGNPGP 467
            .||||..|:.|.|.    ...|:||..|.|||.         |.||.:||||..  |..||.||.
  Fly   434 AGLPGRYGLHGEPGQNVVGPKGEPGLNGQPGLEGYRGDRGEVGLPGDKGLPGEG--YNIVGPPGS 496

Human   468 QGIKGKVGPPGG------RGPKGEKGNEG----LCACEP-GPMGPPGPPGLPGRQGSKGDLGL-- 519
            ||..|..|.||.      ||..||||..|    :|...| ||.|..|..|.||..|::|.:||
  Fly   497 QGPPGFRGLPGDDGYNGLRGLPGEKGLRGDDCPVCNAGPRGPRGQEGDTGYPGSHGNRGAIGLTG 561

Human   520 ----------PGWLGTKGDPGPPGAEGPPGLPGKHGASGPPG--------NKGAKGDMVVSRVKG 566
                      ||..|.||.|||.|.   ||.|||.||:||.|        .||..||...|   |
  Fly   562 PRGVQGLQGNPGRAGHKGLPGPAGI---PGEPGKVGAAGPDGKAIEVGSLRKGEIGDTGDS---G 620

Human   567 HKGERGPDGPPGFPGQPGSHGRDGHAGEKGDPGPPGDHEDATPGGKGFPGPLGPPGKAGPVGPPG 631
            |:|:.|.|      |:.|..|.||..||:|:.|..||:.||     |:.|..|.||:.|..|.||
  Fly   621 HRGDTGDD------GEKGRDGSDGSKGERGETGQRGDYGDA-----GYQGRDGEPGRDGRDGAPG 674

Human   632 LGFPGPP----GERGHPGVPGHPGVRGPDGLKGQKGD----TISCNVTYPGRHGPPGFDGPPGPK 688
            .....|.    ||.|:.|:.|..|..|..|.||.||:    .|..|.   |..|..|:.||.|.|
  Fly   675 RNATTPKVYLIGEPGYDGIKGERGDDGDTGFKGVKGEPNPGQIYDNT---GEPGEDGYTGPKGVK 736

Human   689 GFPGPQGAPGLSGSDGHKGR-----PGTPGTAEIPGPPGFRGDMGDPGFGGEKGSSPVGPPGPPG 748
            |..|.|||.||.|..|.:|.     ||..|....|||.|..|..|.||..|..|.     ||..|
  Fly   737 GAKGEQGAIGLRGEIGDRGPAGEVIPGPVGAKGYPGPTGDYGQQGAPGLPGRDGE-----PGLDG 796

Human   749 SPGVNGQKGIPGDPAF-GHLGPPGKRGLSGVPGIKGPRGDPGCPGAEGPAGIPGFLGLKGPKGRE 812
            ..|..||:|:||.... |.:||||:.|:.|.|      ||.|.||..|.||.||..|:||.:|.:
  Fly   797 GIGYKGQRGVPGQEVIQGEIGPPGRSGIKGFP------GDVGAPGQYGLAGRPGPKGVKGEQGPD 855

Human   813 GHAGFPGVPGPPGHSCE--RGAPGIPGQPGLPGYPGSPGAPGGKGQPGDVGPPGPAGMKGLPGLP 875
            |..|..|:||..|...:  .|.||..||||..|.....||.|.||:.|.:|..|..|.||..|..
  Fly   856 GAVGQTGLPGNKGQRGDFLVGPPGPKGQPGRNGRQAPHGAKGQKGEVGSLGQNGQNGAKGSIGFS 920

Human   876 GRPGAHGPPGLPGIPGPFGDDGLPGPPGPKGPRGLPGFPGFPGERGKPGAEGCPGAKGEPGEKGM 940
            ||.|..|..||.|:||..|..||||..|..|.||..|:.|..|:.|..|..|..|.||..|:.|.
  Fly   921 GRRGLLGNAGLQGLPGSPGIPGLPGMIGEIGERGEIGYNGRQGDIGPRGPNGEFGPKGLSGDDGP 985

Human   941 SGLPGDRGLRGAKGAIGPPGDEGEMAIISQKGTPGEPGPPGDDGFPGERGDKGTPGMQGRRGEP- 1004
            .|.||..||.|.||..|.|            |.||.||..|...:.|.:||.|..|:.|..|.|
  Fly   986 DGYPGANGLPGRKGETGNP------------GFPGRPGAKGVAAYSGIKGDDGESGLTGPIGYPG 1038

Human  1005 -----GRYGPPGFHR----GEPGEKGQPGPPGPPGPPGSTGLRGFIGFPGLPGDQGEPGSPGPPG 1060
                 |:.||.|..:    |..|.||:.|.|||.|.||..||:|..|..||||.||.||.||..|
  Fly  1039 APGAKGQRGPVGDSQPALDGVAGRKGEVGSPGPNGLPGRHGLKGQRGDRGLPGQQGRPGEPGAKG 1103

Human  1061 FSGIDGARGPKGNKGDPASHF----GPPGPKGEPGSPGCPGHFGASGEQGLPGIQGPRGSPGRPG 1121
            ..|..|..|..|.||  |:.|    ||.||:||.|..|..|..|..|:||..|:.|.:|..|..|
  Fly  1104 LGGYPGRNGINGLKG--ATGFPGPQGPKGPQGESGVVGLDGRNGQIGDQGPRGLIGEQGEQGEQG 1166

Human  1122 PPGSSGPPG----------CPGDHGMPGLRGQPGEMGDPGPRGLQGDPGIPGPPGIKGPSGSPGL 1176
            ..|..|.||          ..|..|..||:|:.||.||.||.|..|.||..|..|..|.:|..|.
  Fly  1167 DEGEVGIPGRLENLRDRSFYRGFTGDQGLQGERGEQGDMGPIGFIGPPGAKGERGDIGYAGQLGF 1231

Human  1177 NGLHGLKGQKGTKGASGLHDVGPPGPVGIPGLKGERGDPGSPGI----SPPGPRGKKGPPGPPGS 1237
            :|..||||.:|        |.||.||.||. |..|:||.|..|:    ..||..|:||.|||||.
  Fly  1232 DGAEGLKGFQG--------DQGPRGPPGIT-LPAEKGDEGVAGLDGRAGRPGHFGQKGAPGPPGE 1287

Human  1238 SGPPGPAGATGRAPKDIPDPGPPGDQGPPGPDGPRGAPGPPGLPGSVDLL--RGEPGDCGLPGPP 1300
            :||.|..|..|           |..||||||.|..|.||.||..|...|:  :||.||.|..|..
  Fly  1288 NGPNGAIGHRG-----------PQIQGPPGPQGDVGFPGAPGHNGRHGLIGPKGELGDMGRQGER 1341

Human  1301 GP--------PGPPGPPGYKGFPGCDGKDGQKGPVGFPGPQGPHGFPGPPGEKGLPGPPGRKGPT 1357
            |.        .|..|..|::|.||.||..|::|..|.||..|..|.|||.|..|..|..|..|..
  Fly  1342 GESGYAIVGRQGDIGDIGFQGEPGWDGAKGEQGYPGLPGKNGRVGAPGPRGPTGDAGWGGIDGMD 1406

Human  1358 GLPGPRGEPG--------PPADVDDCPRIPGLPGAPGMRGPEGAMGLPGMRGPSGPGCKGEPGLD 1414
            ||.||:|:||        .|.|       .|.||..|.:|.||..|.||:.|..|.  :|..|..
  Fly  1407 GLVGPKGQPGVTYSYSMARPGD-------RGEPGLDGFQGEEGDGGAPGLIGFQGQ--RGAVGYR 1462

Human  1415 GRRGVDGVPGSPGPPGRKGDTGEDGYPGGP---GPPGPIGDPGPKGFGPGYLGGFLLVLHSQTDQ 1476
            |.:|..|..|:.||.|::||.|..|..|.|   |.|||.|:|.|....| ...||:...|||:..
  Fly  1463 GDQGEVGYTGADGPQGQRGDKGYMGLTGAPGLRGLPGPQGEPAPAPPAP-KSRGFIFARHSQSVH 1526

Human  1477 EPTCPLGMPRLWTGYSLLYLEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSY 1541
            .|.||.....||.||||.......:|..||||.:|||:..|:|:|:..|:|..|||:||.||.|.
  Fly  1527 VPQCPANTNLLWEGYSLSGNVAASRAVGQDLGQSGSCMMRFTTMPYMLCDITNVCHFAQNNDDSL 1591

Human  1542 WLASAAPLP--MMPLSEEAIRPYVSRCAVCEAPAQAVAVHSQDQSIPPCPQTWRSLWIGYSFLMH 1604
            ||::|.|:|  |.|:....:..|:|||.|||...:.:|:|||..|||.||..|..:|.|||:.|.
  Fly  1592 WLSTAEPMPMTMTPIQGRDLMKYISRCVVCETTTRIIALHSQSMSIPDCPGGWEEMWTGYSYFMS 1656

Human  1605 TGAGDQGGGQALMSPGSCLEDFRAAPFLECQGRQGTCHFFANKYSFWLTTVKADLQFSSAPAPDT 1669
            |.....|.||.|:|||||||:|||.|.:||.| .|.|:::....|||||.::...||.. |...|
  Fly  1657 TLDNVGGVGQNLVSPGSCLEEFRAQPVIECHG-HGRCNYYDALASFWLTVIEEQDQFVQ-PRQQT 1719

Human  1670 LKESQAQRQKISRCQVC 1686
            ||....  .|||||.||
  Fly  1720 LKADFT--SKISRCTVC 1734

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
COL4A4	NP_000083.3	7S domain	39..64		7/26 (27%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	61..173		57/117 (49%)
		Triple-helical region	65..1459		633/1569 (40%)
		Cell attachment site. /evidence=ECO:0000255	94..96		0/1 (0%)
		Cell attachment site. /evidence=ECO:0000255	145..147		0/1 (0%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	187..258		37/97 (38%)
		Cell attachment site. /evidence=ECO:0000255	189..191		1/1 (100%)
		Collagen	296..355	CDD:189968	31/82 (38%)
		Cell attachment site. /evidence=ECO:0000255	310..312		0/1 (0%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	369..390		11/20 (55%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	405..451		26/58 (45%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	469..1457		433/1083 (40%)
		Cell attachment site. /evidence=ECO:0000255	724..726		0/1 (0%)
		Cell attachment site. /evidence=ECO:0000255	785..787		0/1 (0%)
		Collagen	829..880	CDD:189968	22/52 (42%)
		Collagen	914..964	CDD:189968	20/49 (41%)
		Cell attachment site. /evidence=ECO:0000255	989..991		0/1 (0%)
		Cell attachment site. /evidence=ECO:0000255	1212..1214		0/1 (0%)
		C4	1466..1570	CDD:279721	46/105 (44%)
		C4	1574..1686	CDD:279721	52/111 (47%)
vkg	NP_001260071.1	Collagen	60..119	CDD:189968	29/58 (50%)
		Collagen	102..152	CDD:189968	24/49 (49%)
		Collagen	277..334	CDD:189968	25/58 (43%)
		Collagen	534..593	CDD:189968	25/61 (41%)
		Collagen	814..871	CDD:189968	28/62 (45%)
		Collagen	957..1014	CDD:189968	26/68 (38%)
		Collagen	990..1049	CDD:189968	24/70 (34%)
		Collagen	1070..1128	CDD:189968	28/59 (47%)
		C4	1515..1624	CDD:128421	49/108 (45%)
		C4	1625..1737	CDD:128421	54/114 (47%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165148265
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E33208_3BAFZ
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D41315at33208
OrthoFinder	1	1.000	-	-		FOG0000443
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100768
Panther	1	1.100	-	-	O	PTHR24023
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	7	6.750