DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Col4a4 and vkg

DIOPT Version :9

Sequence 1:	NP_031761.1	Gene:	Col4a4 / 12829	MGIID:	104687	Length:	1682	Species:	Mus musculus
Sequence 2:	NP_001260071.1	Gene:	vkg / 33726	FlyBaseID:	FBgn0016075	Length:	1940	Species:	Drosophila melanogaster

Alignment Length:	1824	Identity:	739/1824 - (40%)
Similarity:	885/1824 - (48%)	Gaps:	307/1824 - (16%)

- Green bases have known domain annotations that are detailed below.

Mouse    43 NCSVCQCFPEKGSRGHPGPLGPQGPIGPLGPLGPIGIPGEKGERGDSGSPGPPGEK---GDKGPT 104
            |.::|.|...||..|.|||:|..|..||.|.:||.|..|..||:||.|..|..|||   ||.||.
  Fly    34 NTTLCDCKGIKGRMGAPGPIGVPGLEGPAGDIGPPGRAGPLGEKGDVGEYGEQGEKGHRGDIGPK 98

Mouse   105 GVPGFPGVDGVPGHPGPPGPRGKPGVDGYNGSRGDPGYPGERGAPGPGGPPGQPGENGEKGRSVY 169
            |..|:||:.|..|.||.|||||..|.||..|.:|..|.||:.|..||.|.|||.|..||.|....
  Fly    99 GEMGYPGIMGKSGEPGTPGPRGIDGCDGRPGMQGPSGAPGQNGVRGPPGKPGQQGPPGEAGEGGI 163

Mouse   170 ITGGVKGIQGDRGDP---GPPGLPGSRGAQGS---PGPMGHAGAPGLAGPIGHPGS--------- 219
            .:.|.||.:|:.|.|   ||||..|.||::|.   .|..|..|.|||.||.|..|:
  Fly   164 NSKGTKGNRGETGQPGGVGPPGFDGDRGSKGDTGYAGLTGEKGDPGLPGPKGDTGAVSELPYSLI 228

Mouse   220 --PGLKGNPATG----------LKGQRGEPGEVGQRGPPGPTLLVQPPDLSIYKGEKGV---KGM 269
              ||.||.|...          |||.:|..|..|..||.|||            ||:|.   .|:
  Fly   229 GPPGAKGEPGDSLSGVLKPDDTLKGYKGYVGLQGDEGPQGPT------------GEQGAVGRNGL 281

Mouse   270 PGMIGPPGPPGRKGAPG-------VGIKGEKGIPGFPGP---RGEPGSHGPPGFPGFKGIQGAAG 324
            ||..|..|.||.:|.||       .|.||.||.||:.|.   .|.||..|..||.|..|:||.||
  Fly   282 PGARGEIGGPGERGKPGKDGEPGRFGDKGMKGAPGWTGADGLDGSPGERGEDGFTGMPGVQGGAG 346

Mouse   325 EPGLF---------GFLGPKGDLGDRGYPGPPGILLTPAPPLKGVPGDPGPPGYYGEIGDVGLPG 380
            .||::         |.:|.:||:|..|..||||        |.|.||..||.|..|:.||.||.|
  Fly   347 PPGIYDPSLTKSLPGPIGSQGDIGPPGEQGPPG--------LPGKPGRRGPIGLAGQSGDPGLNG 403

Mouse   381 PPGPPGRP--GETCP-GMMGPPGPPGVPGPPGFPGEAGV---PGRLDCAP-GKPGKPGLPGLP-- 436
            ..|||||.  ||... |.:|||||.|.||..|.||..|:   ||:....| |:||..|.|||.
  Fly   404 SRGPPGRSERGEAGDYGFIGPPGPQGPPGEAGLPGRYGLHGEPGQNVVGPKGEPGLNGQPGLEGY 468

Mouse   437 -------GAPGPEGPPGSDV-IYCRPGCPGPMGEKGKVGPPGRRGAKGAKGNKGL----C-TCPP 488
                   |.||.:|.||... |...||..||.|.:|..|..|..|.:|..|.|||    | .|..
  Fly   469 RGDRGEVGLPGDKGLPGEGYNIVGPPGSQGPPGFRGLPGDDGYNGLRGLPGEKGLRGDDCPVCNA 533

Mouse   489 GPMGPPGPPGP---PGRQGSKGDLGLP---GWHGEKGDPGQPGAEGPPGP------PGRPGAMGP 541
            ||.||.|..|.   ||..|::|.:||.   |..|.:|:||:.|.:|.|||      ||:.||.||
  Fly   534 GPRGPRGQEGDTGYPGSHGNRGAIGLTGPRGVQGLQGNPGRAGHKGLPGPAGIPGEPGKVGAAGP 598

Mouse   542 PGHKGEKGDMVISRVKGQKGERGLDGPPGFPGPHGQDGGDGRPGERGDPGPRGDHKDAAPGERGL 606
            .|...|.|.:....: |..|:.|..|..|..|..|:||.||..||||:.|.|||:.||     |.
  Fly   599 DGKAIEVGSLRKGEI-GDTGDSGHRGDTGDDGEKGRDGSDGSKGERGETGQRGDYGDA-----GY 657

Mouse   607 PGLPGPPGRTGPEGPPGLGFPGPPGQRGLPGEPGRPGTRGFDGTKGQKGDSILCNVSYPGKPGLP 671
            .|..|.|||.|.:|.||.....|  :..|.|||      |:||.||::||
  Fly   658 QGRDGEPGRDGRDGAPGRNATTP--KVYLIGEP------GYDGIKGERGD--------------- 699

Mouse   672 GLDGPPGLKGFPGPP---------GAPGMRCPDGQKGQRGKPGMSGIPGPPGFRGDMGDPGIKGE 727
              ||..|.||..|.|         |.||   .||..|.:|..|..|..|..|.||::||.|..||
  Fly   700 --DGDTGFKGVKGEPNPGQIYDNTGEPG---EDGYTGPKGVKGAKGEQGAIGLRGEIGDRGPAGE 759

Mouse   728 KGTSPIGPPGPPGSPGKDGQKGIPGDPAFGDPGPPGERGLPGAPGMKGQKGHPG---CPGAGGPP 789
            ....|:|..|.||..|..||:|.|     |.||..||.||.|..|.|||:|.||   ..|..|||
  Fly   760 VIPGPVGAKGYPGPTGDYGQQGAP-----GLPGRDGEPGLDGGIGYKGQRGVPGQEVIQGEIGPP 819

Mouse   790 GIPGSPGLKGPKGREGSRGFPGIPGSPGHSCERGAPGIPGQPGLPGTPGD-----PGAPGWKGQP 849
            |..|..|..|..|..|..|..|.||..|...|:|..|..||.||||..|.     .|.||.||||
  Fly   820 GRSGIKGFPGDVGAPGQYGLAGRPGPKGVKGEQGPDGAVGQTGLPGNKGQRGDFLVGPPGPKGQP 884

Mouse   850 GDMGPSGPAGMKGLPGLPGLPGADGLRGPPGIPGPNGEDGLPGLPGLKGLPGLPGFPGFPGERGK 914
            |..|...|.|.||..|..|..|.:|..|..|..|.:|..||.|..||:||||.||.||.
  Fly   885 GRNGRQAPHGAKGQKGEVGSLGQNGQNGAKGSIGFSGRRGLLGNAGLQGLPGSPGIPGL------ 943

Mouse   915 PGPDGEPGRKGEVGEKGWPGLKGDLGERGAKGD---RGLPGDAG-EAVTSRKGEPGDAGPPGDGG 975
            ||..||.|.:||:   |:.|.:||:|.||..|:   :||.||.| :......|.||..|..|:.|
  Fly   944 PGMIGEIGERGEI---GYNGRQGDIGPRGPNGEFGPKGLSGDDGPDGYPGANGLPGRKGETGNPG 1005

Mouse   976 FSGERGDKGSSGMRGGRGDPGRDGLPGLHRGQPGIDGPPGPPGPPGPPGSPGLRGVIG-----FP 1035
            |.|..|.||.:...|.:||.|..||             .||.|.||.||:.|.||.:|     ..
  Fly  1006 FPGRPGAKGVAAYSGIKGDDGESGL-------------TGPIGYPGAPGAKGQRGPVGDSQPALD 1057

Mouse  1036 GFPGDQGDPGSPGPPGFPGDDGARGPKGYKGDPASQCGPPGPKGEPGSPGYQGRTGVPGEK---G 1097
            |..|.:|:.|||||.|.||..|.:|.:|.:|.|..| |.||..|..|..||.||.|:.|.|   |
  Fly  1058 GVAGRKGEVGSPGPNGLPGRHGLKGQRGDRGLPGQQ-GRPGEPGAKGLGGYPGRNGINGLKGATG 1121

Mouse  1098 FPGDEGPRGPPGRPGQPGSFGPPGCPGDPGMPGLKGHPGEVGDPGPRGDAGDFGRPGPAGVKGPL 1162
            |||.:||:||               .|:.|:.||.|..|::||.||||..|:.|..|..|.:|.:
  Fly  1122 FPGPQGPKGP---------------QGESGVVGLDGRNGQIGDQGPRGLIGEQGEQGEQGDEGEV 1171

Mouse  1163 GSP-------------GLNGLHGLKGEKGTKGASGLLEMGPPGPMGMPGQKGEKGDPGSPG-ISP 1213
            |.|             |..|..||:||:|.:|     :|||.|.:|.||.|||:||.|..| :..
  Fly  1172 GIPGRLENLRDRSFYRGFTGDQGLQGERGEQG-----DMGPIGFIGPPGAKGERGDIGYAGQLGF 1231

Mouse  1214 PGLPGEKGFPGPPGRPGPP-----------GPAGAPGRAAK----GDIPDPGPPGDRGPPGPDGP 1263
            .|..|.|||.|..|..|||           |.||..|||.:    |....|||||:.||.|..|.
  Fly  1232 DGAEGLKGFQGDQGPRGPPGITLPAEKGDEGVAGLDGRAGRPGHFGQKGAPGPPGENGPNGAIGH 1296

Mouse  1264 RG--VPGPPGSPGNVDLLKGDPGDCGLPGPPGSRGPPGPPGCQGPPGCDGKDGQKGPMGLP---- 1322
            ||  :.|||          |..||.|.||.||..|..|..|.:|..|..|:.|::|..|..
  Fly  1297 RGPQIQGPP----------GPQGDVGFPGAPGHNGRHGLIGPKGELGDMGRQGERGESGYAIVGR 1351

Mouse  1323 -------GLPGPPGLPGAPGEKGLPGPPGRKGPVGPPGCRGEPGPPADVDSCPRIPGLPGVPGPR 1380
                   |..|.||..||.||:|.||.||:.|.||.||.|   ||..|. ....|.|:.|:.||:
  Fly  1352 QGDIGDIGFQGEPGWDGAKGEQGYPGLPGKNGRVGAPGPR---GPTGDA-GWGGIDGMDGLVGPK 1412

Mouse  1381 GPEG-----AMGEPGRRGLPG-PGCKGEPGPDGR------------------RGQDGIPGSPGPP 1421
            |..|     :|..||.||.|| .|.:||.|..|.                  :|:.|..|:.||.
  Fly  1413 GQPGVTYSYSMARPGDRGEPGLDGFQGEEGDGGAPGLIGFQGQRGAVGYRGDQGEVGYTGADGPQ 1477

Mouse  1422 GRKGDTGEAGCPGAP---GPPGPTGDPGPKGFGPGSLSGFLLVLHSQTDQEPACPVGMPRLWTGY 1483
            |::||.|..|..|||   |.|||.|:|.|....|.| .||:...|||:...|.||.....||.||
  Fly  1478 GQRGDKGYMGLTGAPGLRGLPGPQGEPAPAPPAPKS-RGFIFARHSQSVHVPQCPANTNLLWEGY 1541

Mouse  1484 SLLYMEGQEKAHNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLSSAAPLP--MMPL 1546
            ||.......:|..||||.:|||:..|:|:|:..|:|..|||:||.||.|.|||:|.|:|  |.|:
  Fly  1542 SLSGNVAASRAVGQDLGQSGSCMMRFTTMPYMLCDITNVCHFAQNNDDSLWLSTAEPMPMTMTPI 1606

Mouse  1547 SEEEIRSYISRCAVCEAPAQAVAVHSQDQSIPPCPRTWRSLWIGYSFLMHTGAGDQGGGQALMSP 1611
            ...::..|||||.|||...:.:|:|||..|||.||..|..:|.|||:.|.|.....|.||.|:||
  Fly  1607 QGRDLMKYISRCVVCETTTRIIALHSQSMSIPDCPGGWEEMWTGYSYFMSTLDNVGGVGQNLVSP 1671

Mouse  1612 GSCLEDFRAAPFVECQGRQGTCHFFANEYSFWLTTVNPDLQFASGPSPDTLKEVQAQRRKISRCQ 1676
            |||||:|||.|.:||.| .|.|:::....|||||.:....||.. |...|||.....  |||||.
  Fly  1672 GSCLEEFRAQPVIECHG-HGRCNYYDALASFWLTVIEEQDQFVQ-PRQQTLKADFTS--KISRCT 1732

Mouse  1677 VCMK 1680
            ||.:
  Fly  1733 VCRR 1736

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Col4a4	NP_031761.1	7S domain. /evidence=ECO:0000250\|UniProtKB:P53420	31..56		5/12 (42%)
		Collagen	54..111	CDD:189968	29/59 (49%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	56..255		98/228 (43%)
		Triple-helical region. /evidence=ECO:0000250\|UniProtKB:P53420	57..1451		627/1577 (40%)
		Cell attachment site. /evidence=ECO:0000255	86..88		0/1 (0%)
		Cell attachment site. /evidence=ECO:0000255	137..139		0/1 (0%)
		Cell attachment site. /evidence=ECO:0000255	181..183		0/1 (0%)
		Collagen	291..348	CDD:189968	28/68 (41%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	379..1453		472/1205 (39%)
		Cell attachment site. /evidence=ECO:0000255	587..589		1/1 (100%)
		Cell attachment site. /evidence=ECO:0000255	593..595		1/1 (100%)
		Cell attachment site. /evidence=ECO:0000255	716..718		1/1 (100%)
		Collagen	805..858	CDD:189968	25/57 (44%)
		Collagen	889..948	CDD:189968	28/61 (46%)
		Cell attachment site. /evidence=ECO:0000255	980..982		0/1 (0%)
		Cell attachment site. /evidence=ECO:0000255	992..994		0/1 (0%)
		Cell attachment site. /evidence=ECO:0000255	1144..1146		1/1 (100%)
		Collagen	1338..1405	CDD:189968	31/72 (43%)
		C4	1458..1562	CDD:279721	48/105 (46%)
		C4	1566..1679	CDD:279721	53/112 (47%)
vkg	NP_001260071.1	Collagen	60..119	CDD:189968	30/58 (52%)
		Collagen	102..152	CDD:189968	26/49 (53%)
		Collagen	277..334	CDD:189968	22/56 (39%)
		Collagen	534..593	CDD:189968	24/58 (41%)
		Collagen	814..871	CDD:189968	25/56 (45%)
		Collagen	957..1014	CDD:189968	22/56 (39%)
		Collagen	990..1049	CDD:189968	27/71 (38%)
		Collagen	1070..1128	CDD:189968	27/58 (47%)
		C4	1515..1624	CDD:128421	51/108 (47%)
		C4	1625..1737	CDD:128421	54/116 (47%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167838355
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E33208_3BAFZ
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000443
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100768
Panther	1	1.100	-	-	O	PTHR24023
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	6	5.740