DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Col4a3 and vkg

DIOPT Version :9

Sequence 1:	NP_031760.2	Gene:	Col4a3 / 12828	MGIID:	104688	Length:	1669	Species:	Mus musculus
Sequence 2:	NP_001260071.1	Gene:	vkg / 33726	FlyBaseID:	FBgn0016075	Length:	1940	Species:	Drosophila melanogaster

Alignment Length:	1826	Identity:	764/1826 - (41%)
Similarity:	888/1826 - (48%)	Gaps:	256/1826 - (14%)

- Green bases have known domain annotations that are detailed below.

Mouse     7 PRFLVFL--LLTLLLLLAA--SPVASKGCVCKGKGQCLCAGTKGEKGEKGVPGSPGFPGQKGFPG 67
            ||.|..|  ||.::.||.:  |...:.|.:| ....|.|.|.||..|..|..|.||..|..|..|
  Fly     3 PRDLRHLSGLLGVVYLLGSLVSVTLADGKIC-NTTLCDCKGIKGRMGAPGPIGVPGLEGPAGDIG 66

Mouse    68 PEGLPGPQGPKGSPGLPGLTGPKGIRGITGLPGFAGPPGLPGLPGHPGPRGLAGLPGCNGSKGEQ 132
            |.|..||.|.||..|..|..|.||.||..|..|..|.||:.|..|.||..|..|:.||:|..|.|
  Fly    67 PPGRAGPLGEKGDVGEYGEQGEKGHRGDIGPKGEMGYPGIMGKSGEPGTPGPRGIDGCDGRPGMQ 131

Mouse   133 GFPGFPGTPGYAGLPGPDGLKGQKGEPAQGEDRGFNGKGDPGPPGVPGFQGFPGLPGFPGPAGPP 197
            | |.  |.||..|:.||.|..||:|.|.:..:.|.|.||..|..|..|..|..|.|||.|..|..
  Fly   132 G-PS--GAPGQNGVRGPPGKPGQQGPPGEAGEGGINSKGTKGNRGETGQPGGVGPPGFDGDRGSK 193

Mouse   198 GPPGFFGL------PGAMGPRGPKGHMGD---SVIGQKGERGMKG--LTGPPGPPGTVIFTLTQP 251
            |..|:.||      ||..||:|..|.:.:   |:||..|.:|..|  |:|...|..|:
  Fly   194 GDTGYAGLTGEKGDPGLPGPKGDTGAVSELPYSLIGPPGAKGEPGDSLSGVLKPDDTL------- 251

Mouse   252 YNKSDFKGEKGDEGERGEPGPPGPSGPPG----DSYGSEKGAPGEPGPRGKPGKDGAP------- 305
                  ||.||..|.:|:.||.||:|..|    :.....:|..|.||.||||||||.|
  Fly   252 ------KGYKGYVGLQGDEGPQGPTGEQGAVGRNGLPGARGEIGGPGERGKPGKDGEPGRFGDKG 310

Mouse   306 --GFPGTEGAKGNRGFPGLRGE---AGIKGRKGDIGPPGFPGPTEYYDAYLEKGERGMPGLPGPK 365
              |.||..||.|..|.||.|||   .|:.|.:|..||||.      ||..|.|      .||||.
  Fly   311 MKGAPGWTGADGLDGSPGERGEDGFTGMPGVQGGAGPPGI------YDPSLTK------SLPGPI 363

Mouse   366 GAR---GPQGPSGPPGVPGSPGLSRP-GLRGPIGWPGLKGSKGERGPPGKDTVGPPGPLGCPGSP 426
            |::   ||.|..||||:||.||...| ||.|..|.|||.||   |||||:...|..|..|..|.|
  Fly   364 GSQGDIGPPGEQGPPGLPGKPGRRGPIGLAGQSGDPGLNGS---RGPPGRSERGEAGDYGFIGPP 425

Mouse   427 GPPGPPGPPGCPGDIVFKCSPGEHGMPGDTGPPGVPGLDGPKGEPGSPCTECHCFPGPPGVPGFP 491
            ||.||||..|.||..      |.||.||.       .:.|||||||        ..|.||:.|:.
  Fly   426 GPQGPPGEAGLPGRY------GLHGEPGQ-------NVVGPKGEPG--------LNGQPGLEGYR 469

Mouse   492 GLDGIKGIPGGRGVPGLKGN-PGSPGSAGLPGFAGFPGDQGHPGLK---GDKG----DTPLPWGQ 548
            |..|..|:||.:|:||...| .|.|||.|.|||.|.|||.|:.||:   |:||    |.|
  Fly   470 GDRGEVGLPGDKGLPGEGYNIVGPPGSQGPPGFRGLPGDDGYNGLRGLPGEKGLRGDDCP----- 529

Mouse   549 VGNPGDPGLRGLPGRKGFDGTPGGPGAKGPPGPQGEPALS---GRKGDQGPPGPPGFPGPPGPAG 610
            |.|.|..|.||..|..|:.|:.|..||.|..||:|...|.   ||.|.:|.|||.|.||.||..|
  Fly   530 VCNAGPRGPRGQEGDTGYPGSHGNRGAIGLTGPRGVQGLQGNPGRAGHKGLPGPAGIPGEPGKVG 594

Mouse   611 PAGPPG---------------------YGPQGEPGPKGAQGVPGVLGPPGEAGLKGEPSTSTPDL 654
            .|||.|                     .|..|:.|.||..|..|..|..||.|.:|:..    |.
  Fly   595 AAGPDGKAIEVGSLRKGEIGDTGDSGHRGDTGDDGEKGRDGSDGSKGERGETGQRGDYG----DA 655

Mouse   655 GPPGPPGPPGQAGPRGLPGL------------PGPVG-------KCDPGLPGPDGEP-------G 693
            |..|..|.||:.|..|.||.            ||..|       ..|.|..|..|||       .
  Fly   656 GYQGRDGEPGRDGRDGAPGRNATTPKVYLIGEPGYDGIKGERGDDGDTGFKGVKGEPNPGQIYDN 720

Mouse   694 IPEAGCPGPPGPKGNQGFPGTKGSPGCPGEMGKPGRPGE--PGIPGAKGEPS-VGRPGKPGKPGF 755
            ..|.|..|..||||.:|..|.:|:.|..||:|..|..||  ||..||||.|. .|..|:.|.||.
  Fly   721 TGEPGEDGYTGPKGVKGAKGEQGAIGLRGEIGDRGPAGEVIPGPVGAKGYPGPTGDYGQQGAPGL 785

Mouse   756 PGERGNAGENGDIGLPGLPGLP---------GTPGRGGLDGPPGDPGQPGSPGAKGSPG-RCIPG 810
            ||..|..|.:|.||..|..|:|         |.|||.|:.|.|||.|.||..|..|.|| :.:.|
  Fly   786 PGRDGEPGLDGGIGYKGQRGVPGQEVIQGEIGPPGRSGIKGFPGDVGAPGQYGLAGRPGPKGVKG 850

Mouse   811 PRGTQGLPGLNGLKGQPGRRGD-----TGPKGDPGIPGMDRSGVPGDPGPPGTPGCPGEMGPPGQ 870
            .:|..|..|..||.|..|:|||     .||||.||     |:|.....|..|..|..|.:|..||
  Fly   851 EQGPDGAVGQTGLPGNKGQRGDFLVGPPGPKGQPG-----RNGRQAPHGAKGQKGEVGSLGQNGQ 910

Mouse   871 KGYPGAPGFPGPPGEKGEVGMMGYPGTTGPPGLPGKPGSQGQRGSLGIPGMKGEKGRPGAKGERG 935
            .|..|:.||.|..|..|..|:.|.||:.|.|||||..|..|:||.:|..|.:|:.|..|..||.|
  Fly   911 NGAKGSIGFSGRRGLLGNAGLQGLPGSPGIPGLPGMIGEIGERGEIGYNGRQGDIGPRGPNGEFG 975

Mouse   936 EKGKPGPSQTTLLKGDKGEPGLKGFVGNPGEKGNRGNPGLPGPKGLEGLPGLPGPPGPRGDTGSR 1000
            .||..|..      |..|.||..|..|..||.||.|.||.||.||:....|:.|..|..|.||..
  Fly   976 PKGLSGDD------GPDGYPGANGLPGRKGETGNPGFPGRPGAKGVAAYSGIKGDDGESGLTGPI 1034

Mouse  1001 GNPGRPGPHGMPGSMGIM--GVPGPKGRKGTSGLPGLAGRPGLTGIHGPQGDKGEPGYSEGARPG 1063
            |.||.||..|..|.:|..  .:.|..||||..|.||..|.||..|:.|.:||:|.||  :..|||
  Fly  1035 GYPGAPGAKGQRGPVGDSQPALDGVAGRKGEVGSPGPNGLPGRHGLKGQRGDRGLPG--QQGRPG 1097

Mouse  1064 PPGPKGDPGLPGD---KGKKGERGVPGPPGQSGPAGPDGAPGSPGSPGHPGKPGPAGDLGLKGQK 1125
            .||.||..|.||.   .|.||..|.|||.|..||.|..|..|..|..|..|..||.|.:|.:|::
  Fly  1098 EPGAKGLGGYPGRNGINGLKGATGFPGPQGPKGPQGESGVVGLDGRNGQIGDQGPRGLIGEQGEQ 1162

Mouse  1126 GFPGPPGSTGPPGP-------------PGLPGLPGPMGMRGDQGRDGIPGPPGEKGETGLLG--- 1174
            |..|..|..|.||.             .|..||.|..|.:||.|..|..||||.|||.|.:|
  Fly  1163 GEQGDEGEVGIPGRLENLRDRSFYRGFTGDQGLQGERGEQGDMGPIGFIGPPGAKGERGDIGYAG 1227

Mouse  1175 --------------AYPGPKGSPGV--PGAKGDRGVPGLSGLPGRKGVMGDVGPQGPPGTAGLPG 1223
                          ...||:|.||:  |..|||.||.||.|..||.|..|..|..||||..|..|
  Fly  1228 QLGFDGAEGLKGFQGDQGPRGPPGITLPAEKGDEGVAGLDGRAGRPGHFGQKGAPGPPGENGPNG 1292

Mouse  1224 PPGLPGAII---PGPKGDRGLPGLRGNPGEPGPPGPPGPIGKGIKGDKGFMGPPGPKG--LPGTV 1283
            ..|..|..|   |||:||.|.||..|:.|..|..||     ||..||.|..|..|..|  :.|..
  Fly  1293 AIGHRGPQIQGPPGPQGDVGFPGAPGHNGRHGLIGP-----KGELGDMGRQGERGESGYAIVGRQ 1352

Mouse  1284 GDMGPPGFPGAPGTPGLPGVRGDPGFPGFPGIKGEKGNPGFLGPIGHP---GPVGPKGPPGPRGK 1345
            ||:|..||   .|.||..|.:|:.|:||.||..|..|.||..||.|..   |..|..|..||:|:
  Fly  1353 GDIGDIGF---QGEPGWDGAKGEQGYPGLPGKNGRVGAPGPRGPTGDAGWGGIDGMDGLVGPKGQ 1414

Mouse  1346 PGTLKVISL--PGSPGPPGVPGQPGMKGD---PGPLGLPGIPGPCGPRGKPGKDGKPGTPGPAGT 1405
            ||.....|:  ||..|.||:.|..|.:||   ||.:|..|..|..|.||..|:.|..|..||.|.
  Fly  1415 PGVTYSYSMARPGDRGEPGLDGFQGEEGDGGAPGLIGFQGQRGAVGYRGDQGEVGYTGADGPQGQ 1479

Mouse  1406 KGNKGLKGQQGPPGLDGLPGLKGNPGDRGTPA-TGTRMRGFIFTRHSQTTAIPSCPEGTQPLYSG 1469
            :|:||..|..|.|||.||||.:|.|    .|| ...:.|||||.||||:..:|.||..|..|:.|
  Fly  1480 RGDKGYMGLTGAPGLRGLPGPQGEP----APAPPAPKSRGFIFARHSQSVHVPQCPANTNLLWEG 1540

Mouse  1470 FSLLFVQGN---KRAHGQDLGTLGSCLQRFTTMPFLFCNINNVCNFASRNDYSYWLSTPALMPMD 1531
            :||   .||   .||.|||||..|||:.||||||::.|:|.|||:||..||.|.||||...|||.
  Fly  1541 YSL---SGNVAASRAVGQDLGQSGSCMMRFTTMPYMLCDITNVCHFAQNNDDSLWLSTAEPMPMT 1602

Mouse  1532 MAPISGRALEPYISRCTVCEGPAMAIAVHSQTTAIPPCPQDWVSLWKGFSFIMFTSAGSEGAGQA 1596
            |.||.||.|..|||||.|||.....||:|||:.:||.||..|..:|.|:|:.|.|.....|.||.
  Fly  1603 MTPIQGRDLMKYISRCVVCETTTRIIALHSQSMSIPDCPGGWEEMWTGYSYFMSTLDNVGGVGQN 1667

Mouse  1597 LASPGSCLEEFRASPFIECHGRGTCNYYSNSYSFWLASLNPERMFRKPIPSTVKAGDLEKIISRC 1661
            |.|||||||||||.|.|||||.|.||||....||||..:..:..|.:|...|:|| |....||||
  Fly  1668 LVSPGSCLEEFRAQPVIECHGHGRCNYYDALASFWLTVIEEQDQFVQPRQQTLKA-DFTSKISRC 1731

Mouse  1662 QVCMKK 1667
            .||.::
  Fly  1732 TVCRRR 1737

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Col4a3	NP_031760.2	7S domain. /evidence=ECO:0000250\|UniProtKB:Q01955	29..42		3/12 (25%)
		Collagen	42..94	CDD:189968	23/51 (45%)
		Triple-helical region	43..1436		626/1549 (40%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	44..473		191/459 (42%)
		Collagen	288..343	CDD:189968	32/66 (48%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	500..1439		428/1065 (40%)
		Collagen	712..765	CDD:189968	25/55 (45%)
		Cell attachment site. /evidence=ECO:0000255	830..832		1/1 (100%)
		Cell attachment site. /evidence=ECO:0000255	994..996		0/1 (0%)
		Collagen	998..1055	CDD:189968	25/58 (43%)
		Cell attachment site. /evidence=ECO:0000255	1152..1154		0/1 (0%)
		Cell attachment site. /evidence=ECO:0000255	1304..1306		0/1 (0%)
		Collagen	1377..1436	CDD:189968	26/58 (45%)
		Epitope recognized by Goodpasture antibodies. /evidence=ECO:0000250\|UniProtKB:Q01955	1425..1443		5/18 (28%)
		C4	1445..1551	CDD:279721	62/108 (57%)
		Required for the anti-angiogenic activity of tumstatin. /evidence=ECO:0000250\|UniProtKB:Q01955	1478..1556		48/80 (60%)
		C4	1555..1665	CDD:279721	56/109 (51%)
		Required for the anti-tumor cell activity of tumstatin. /evidence=ECO:0000250\|UniProtKB:Q01955	1609..1627		12/17 (71%)
vkg	NP_001260071.1	Collagen	60..119	CDD:189968	27/58 (47%)
		Collagen	102..152	CDD:189968	24/52 (46%)
		Collagen	277..334	CDD:189968	25/56 (45%)
		Collagen	534..593	CDD:189968	27/58 (47%)
		Collagen	814..871	CDD:189968	24/56 (43%)
		Collagen	957..1014	CDD:189968	26/62 (42%)
		Collagen	990..1049	CDD:189968	27/58 (47%)
		Collagen	1070..1128	CDD:189968	28/59 (47%)
		C4	1515..1624	CDD:128421	65/111 (59%)
		C4	1625..1737	CDD:128421	57/112 (51%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	131	1.000	Domainoid score	I5118
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D41315at33208
OrthoFinder	1	1.000	-	-		FOG0000443
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100768
Panther	1	1.100	-	-	O	PTHR24023
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	8	7.880