DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Cacna1a and NaCP60E

DIOPT Version :9

Sequence 1:	XP_006530650.1	Gene:	Cacna1a / 12286	MGIID:	109482	Length:	2464	Species:	Mus musculus
Sequence 2:	NP_001261172.1	Gene:	NaCP60E / 37981	FlyBaseID:	FBgn0085434	Length:	2896	Species:	Drosophila melanogaster

Alignment Length:	2950	Identity:	640/2950 - (21%)
Similarity:	1081/2950 - (36%)	Gaps:	929/2950 - (31%)

- Green bases have known domain annotations that are detailed below.

Mouse   159 RQNCL---TVNRSLFLFSEDNVVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPM 220
            |:|.:   |..:|||||...:..|:....|.....|:|.::|||:.|||.||            |
  Fly    93 RKNYIHRFTGTKSLFLFYPWSPARRVCVYIATNQFFDYCVMATILFNCIFLA------------M 145

Mouse   221 SERLDDTEPYFIGIFCFEAGIKIVALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEF-DLRT 284
            :|.:::.|..|:.|:..|..|||:|.||..:|.:||||.||.:||||:.:| .||:|.|. :|..
  Fly   146 TETVEEAEYIFLAIYSIEMVIKIIAKGFLLNKYTYLRNPWNWLDFVVITSG-YATIGMEVGNLAG 209

Mouse   285 LRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFE 349
            ||..||||.||.||.:|.|:.::.:::.:...|.::..|..|.:::||:..|:.|||:....|..
  Fly   210 LRTFRVLRALKTVSIMPGLKTIINALLHSFRQLAEVMTLTIFCLMVFALFALQVYMGELRNKCVR 274

Mouse   350 E------------------GTDD---IQGESPAPCGTEEPARTCPNGTKCQPYWEGPNNGITQFD 393
            :                  .||:   .:.|.|..||....||.||....|....|.||:|.|.||
  Fly   275 QVPTDWTNVSHTDWQIWVNDTDNWLYDEDELPVLCGNLTGARHCPFEYVCLCVGENPNHGYTNFD 339

Mouse   394 NILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKER 458
            |.::::||.||.||::.|.: :||...|:....:..:|..::..|||:::||:|.|::..:.:|.
  Fly   340 NFMWSMLTTFQLITLDYWEN-VYNMVLATCGPMSVSFFTVVVFFGSFYLINLMLAVVALSYEEEA 403

Mouse   459 E--RVENRRAFLKLRRQQQIE--------RELN-GYMEWISKAEEVILAE--------------- 497
            |  ..|.::..|..|......        ::|| ...:.|...:.|:||.
  Fly   404 EITNEERKKDLLDHRDDSTFSFDPSVLNVKKLNKNNKKKIDSRKGVLLASYSKKKTRRKKTKGGK 468

Mouse   498 -------------DET---------------------------------DVEQRH---------- 506
                         |:.                                 .:||:|
  Fly   469 EGGTNGNGNGSNGDDNKSHSATPSPGPSPRHSATERPSALTMQAQKQYQQMEQQHKLAKSGSGGS 533

Mouse   507 -----PFDVG--ALRRATLKKSKTDLLNPEEAEDQLADIASVGSPFARAS--------------- 549
                 |...|  :.:.:.:.....::|.|.:.:.||  ||:.|.|.:.:|
  Fly   534 NNPMAPTPKGRISFQDSGMGVKNPNMLYPSDYKGQL--IANSGQPSSNSSGVNRESSQDDSGVVD 596

Mouse   550 ----------------------------IKS----AKLENSTFF--HKK---------------- 564
                                        ||.    |:::|...:  |::
  Fly   597 DHEEQDTTNDMGHVSTVELALSPREVRLIKCNGNIARIKNHNVYALHQEFSSEVVVIDDLPDRNC 661

Mouse   565 ----------ERRMRFY--IRRMVKTQAFYWTVLSLVALNTLCVAIVHYNQPEWLSDFLYYAEFI 617
                      |..::|.  :.::|:...|...:...:.|||..:|:.|:...|...:.|.....:
  Fly   662 DRCVHWCTDYESWLQFQNCLYKVVRDPLFELAITLCIVLNTAFLAMEHHGMSESFRNALDVGNKV 726

Mouse   618 FLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRI 682
            |..:|..|..:|:..| ::.:|...:|.||..::..|:.::|:.::.     |:||||.|||||:
  Fly   727 FTSIFTFECIVKLMAL-SKDFFLCGWNIFDLLIVTASLLDIIFELVD-----GLSVLRGLRLLRV 785

Mouse   683 FKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQF---NFD-EGTPPTNF 743
            .|:.:.|.:::.|:..:::::.::.:|..:|.:.|.:||::|||||...:   .|| :..|..||
  Fly   786 LKLAQSWTTMKVLLSIIISTIGALGNLTLILVIVIYIFAVIGMQLFSKDYTPEKFDPDPVPRWNF 850

Mouse   744 DTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFIVLTLFGNYTLLNVFLAIAVDN 808
            :.|..:.|.:|:||.|| |.|.::|.::::........|:|:...|.: ||:.:||:|||:.: |
  Fly   851 NDFFHSFMMIFRILCGE-WIEPLWDCMRAEEEQGASTCFAIFLPTLVM-GNFMVLNLFLALLL-N 912

Mouse   809 LANAQELTKDEQEEEEAANQKLALQKAKEVAEVSPLSAANMSIAVKEQQKNQKPTKSVWEQRTSE 873
            ..|::||...::|..|.:....::::.:::              ::::::.:|..|   |::.:|
  Fly   913 SFNSEELKSKKEEVGEESKLARSIERVRDL--------------IRKKRQERKDRK---ERKFAE 960

Mouse   874 MRKQNLLASREA----LYGDAA---ERWPTPYARPLRPDVKTH-------LDRPL---------- 914
            ..:|.:|.:::|    |...||   ||...|   .:..:.|.|       ::||:
  Fly   961 KFQQIVLDAQQAHAQTLSHQAAVGLERGDKP---GVLAETKFHRLSYQESMNRPVSGSDFGFQIP 1022

Mouse   915 -------VVDPQENRNNNTNKSRAPEALRPTARPRESARD------PDARRAWPGSPERAPGREG 966
                   :||..|..:...    .||.::..|.|.....|      ..|..|..|....:....|
  Fly  1023 LHDGLHTIVDGLEYDDTGD----LPEQIQLQAHPLPPTSDSMPPTYESAMMATTGGSFSSVNGNG 1083

Mouse   967 ------PYGRESEPQQR--------EHAPPREHAPWD-----------------ADTERAKAGDA 1000
                  |:.:.....|.        :....||.|.:.                 |:.:|:..|..
  Fly  1084 TCQNLTPFVQAERRLQHQISSGVSTQQYDSREEATYTESIELLGQYNSTDTDPYANDQRSGCGSF 1148

Mouse  1001 PRRHTHRPVAEGEPRRHRARRRPGDEPDDRP---------ERRPRPRDATRPARAADGEG----- 1051
            .|         |:..:..:.||.|.|..|..         .|.|..|.:......:.|:.
  Fly  1149 NR---------GDSLQDNSSRRYGSEEHDEAFLKYQKSLLTRSPSYRKSLDRLSQSSGQSQRSLL 1204

Mouse  1052 -DDGERKRRHRHGPPAH-----------------------DDRE-----------------RR-- 1073
             .:....|||..|...:                       |.:|                 ||
  Fly  1205 KSEEAEMRRHSSGQSLNSMSIEQDELLSQQGNLREELLNCDQKELFQFLQEEEELQKGTKLRRIS 1269

Mouse  1074 --HRRRKENQGSGVPVSGPNLSTT---RPIQ------QDLGRQDLPLAEDLDNMKNNKLATGE-- 1125
              .|.|:.:...|.|.:...:..:   ..||      :::.|....|...|.|:.....|..|
  Fly  1270 NVMRSRRPSSQMGQPENETMVEHSEFDNIIQSFEKELEEIKRSTTSLERKLSNLSEPSPAADEAT 1334

Mouse  1126 ------------------------PASPHDSLGHSGLPPSPAKIGNSTN---------------- 1150
                                    |.:|:||...|.:|.....:..:..
  Fly  1335 KAIMEHIAIITGASERSAADEVVLPLNPYDSYDLSSVPRRSQSVSAAAQRQSVKLKRRSLEKQRK 1399

Mouse  1151 --------------------PGPAL------ATNPQNAASRRTP--------------------- 1168
                                |..|:      ||:...|...::|
  Fly  1400 IDEDFSISNEIRKICDQIHAPFVAMEAMAVAATSASQAQPNQSPFLRRKVDPFTVQFDRFKRLSL 1464

Mouse  1169 -----------------------------NNP--------------------------GNPSNPG 1178
                                         :.|                          |..:.|.
  Fly  1465 IERVEEVPEEEKPISTLRIESEKMPRKFLHGPDQLRLDSLSLKSTNSYENLLIQKQKLGMATPPA 1529

Mouse  1179 PPKTPENSL--IVTNPSSTQPNSAKT----ARKPEHM----AVEIPPACPP-------------- 1219
            .|.||..||  .:..|:..|.:|.||    |...||.    |..|..|..|
  Fly  1530 VPATPPTSLKSSIEPPTLAQISSLKTTPPLAALTEHQQHFHATSIQAAPTPAHTHAHSQAHAHSM 1594

Mouse  1220 ------LNHTVVQVNKNAN----------------PDPLPKKEEEKKEEEEADPGEDGPKPMPPY 1262
                  :.|....::|.|:                ...|.....:|.|:.::...:...||
  Fly  1595 AGQRRRMEHPQSTLDKAASFQSARTESHSSGAADASSALALAMAQKTEQSQSTAPDATQKP---- 1655

Mouse  1263 SSMFILSTTNPLRRLCHYILNLRY----------------------------------------- 1286
             |.|...|..|...|..|:.:|..
  Fly  1656 -SAFTRLTEKPWHCLVSYVDDLTVGGRRNSQGAYNDPMTFPSYGATKAAKVPDDCFPQKCYDHFY 1719

Mouse  1287 ---------------------------------FEMCILMVIAMSSIALAAED-PVQPNAPRNNV 1317
                                             ||..:|::|..|||.|..|| .:..|.....|
  Fly  1720 FRCPWFMSCMDTQSAKHWTRVRTAVLTVVDTPAFEWFVLVLIFASSITLCFEDINLDKNKTLKRV 1784

Mouse  1318 LRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYFRDLWNILDFIVVSGALVAFAFTSSTRGNSKGK 1382
            |.:.::.|..:|..||::|.:.||.    ..||...|.|||||:|..::.:...       .:.:
  Fly  1785 LYWINFSFCLIFVVEMILKWLALGF----SKYFTSFWTILDFIIVFVSVFSLLI-------EENE 1838

Mouse  1383 DINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGK 1447
            ::..::|||.||.||||:.|.|...::.|.:.::.::.::||:|:|.::|..||:::.||.|.||
  Fly  1839 NLKVLRSLRTLRALRPLRAISRWQGMRIVVNALMYAIPSIFNVLLVCLVFWLIFSIMGVQFFGGK 1903

Mouse  1448 FFHCTDESKEF--ERDCRGKYLLYEKNEVKARDREWKKYEFHYDNVLWALLTLFTVSTGEGWPQV 1510
            ||.|.:|..|.  ..:...|:...|:|..      |...:..:|:|....|.|..|:|.|||.:|
  Fly  1904 FFKCVNEMGELLPITEVNDKWDCIEQNYT------WINSKITFDHVGMGYLALLQVATFEGWMEV 1962

Mouse  1511 LKHSVDATFENQGPSPGYRMEMSIFYVVYFVVF----PFFFVNIFVALIIITFQEQGDK----MM 1567
            :..:|||...:..|    :.|.:::..:|||:|    .||.:|:|:.:||..|.....|    ::
  Fly  1963 MADAVDARGVDLQP----QREANLYAYIYFVIFIVCGSFFTLNLFIGVIIDNFNMLKKKYEGGVL 2023

Mouse  1568 EEYSLEKNERACIDFAISAKPLTRHMPQN--KQSFQYRMWQFV---VSPPFEYTIMAMIALNTIV 1627
            |.:..|..:.    :..:.|.|.|..||.  |:...:.:..|.   .|..||..|..:|.||.:.
  Fly  2024 EMFLTESQKH----YYTAMKKLGRKKPQKVIKRPINHFLAMFYDLSNSRRFEIAIFVLIFLNMLT 2084

Mouse  1628 LMMKFYGASVAYENALRVFNIVFTSLFSLECVLKVMAFGILNYFRDAWNIFDFVTVLGSITDILV 1692
            :.::.|....|....|.|.|..||::|.||.::|::... .:||...||:|||:.||.||..||:
  Fly  2085 MGIEHYDQPHAVFFILEVSNAFFTTVFGLEAIVKIVGLR-YHYFTVPWNVFDFLLVLASIFGILM 2148

Mouse  1693 TE--FGNPNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAI 1755
            .:  ...|.:...|..:|:||..|:::|::....||.||:..|.|..||..:..|:.::.|||||
  Fly  2149 EDIMIDLPISPTLLRVVRVFRIGRILRLIKAAKGIRKLLFALVVSLPALFNIGALLGLITFIYAI 2213

Mouse  1756 IGMQVFGNIGIDGEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKP-CD 1819
            :||.:|||:.:.|..:|.          .||:||.:::.||||..|...|::::.|.:...| ||
  Fly  2214 LGMSLFGNVKLQGALDDM----------VNFQTFGRSMQLLFRLMTSAGWNDVLESLMIQPPDCD 2268

Mouse  1820 K-NSGILTADCGNE-FAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSI-LGPHHLDEYVR 1881
            . ..|....:||:. .|..||.|||.:...:::|:::|:|::||....::..| :....|:.:..
  Fly  2269 PFIHGHTNGNCGHPLLAITYFTSFIIISYMIVINMYIAIILENFNQAHQEEEIGIVEDDLEMFYI 2333

Mouse  1882 VWAEYDPAACGRIHYKDMYSLLRVISPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMA 1946
            .|::|||.|...||:..:...:..:.||||:.|  |:.||   |:..:||::..|.:|....|.|
  Fly  2334 RWSKYDPHATQFIHFSQLSDFIASLDPPLGISK--PNNVA---LVSFNLPISKGNKIHCLDILHA 2393

Mouse  1947 LIRTALDIKIAKGGAD--------KQQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKI 2003
            |::..|      |..:        ::|||.:.:|:    :|  ::|.|:::    .||.:...:.
  Fly  2394 LVKHVL------GHVEETDNFKQLQEQMDVKFKKQ----FP--TRKELEIV----SSTRIWKRQE 2442

Mouse  2004 YAAMMIM----EYYRQSKAKKLQAMREEQNRTPLMFQRMEPPSPTQEGGPS--QNALPS-----T 2057
            .||..|.    ||.|:.:.|      |..|         ...|.||...|.  |:.|.:     .
  Fly  2443 KAAKTIQTGWKEYLRRKREK------ERSN---------SGDSATQTSSPGGWQSKLSALNFFHL 2492

Mouse  2058 QLDPGGGLMAHEGGMKESPSWVTQRAQEMFQKTGTW---------------------SPERG--- 2098
            |:...|...:.....::|..  ...|.::.:..|.|                     :.|.|
  Fly  2493 QVSRRGTACSSRASSRKSSR--ASDASDLSELAGPWLNLPLMLVSGADEVVKDIKQQNDELGKRG 2555

Mouse  2099 --------------------------------PPIDMPNSQPNSQSVEMREMGTDGYSDSEHYLP 2131
                                            |.:....:..|:.:.......|.|.:.|....|
  Fly  2556 SIFVEAPRASRRRSFYNFFLRHQDAVDDSLTSPSVHRKTAMNNTTNTTSNSASTSGTASSTATAP 2620

Mouse  2132 MEGQTRAASMPRLPAENQRRRG-----RPRGNDLSTISDTSPMKRSASVLGPKARRLD------- 2184
            ..|...||:.......:|...|     |.|.:  |.|....|::|..|  ...|.|::
  Fly  2621 ATGCGPAATSASDSDRHQAVGGGSAPSRKRAS--SFIRKKPPLERGLS--AQSALRVNKNAFVSE 2681

Mouse  2185 ----DYSLERVPPEENQRYH--QRRRDRG-------HRTSERSLGRYTDVD----------TGLG 2226
                :..:.|..||:....|  ..|.|..       ||.||    .|.:.|          .|.|
  Fly  2682 ASAPEVIVTRPSPEQQTHPHSLSLRPDNATLVHVLVHRESE----EYKEEDESSPSVSGNGNGFG 2742

Mouse  2227 TDLSM-----------TTQS-------------------------GDLPSKDRDQDRGRPKDRKH 2255
            ..|.|           ||.|                         ||..|...| |.|.|.|...
  Fly  2743 VGLDMLSKQPPPQIRITTGSVESSMDTCAMPTVQIMVDSPKDPPRGDFSSAPID-DVGAPIDVNV 2806

Mouse  2256 RPHHHHHHHHHHP------------------PAPDRDRYAQERPDTGRARAREQRWSRSPSEGRE 2302
            :.......:.::|                  ..|||.|..........:..|..|.|||.|....
  Fly  2807 QGDTSQVFYDYNPEKATDDQGNGQDETAQFESLPDRQRXPPVNTPLRDSEKRSSRGSRSSSSSTS 2871

Mouse  2303 --HTTHRQGSSSVSGSPAPSTSGTS 2325
              .:.....|||.:.:.|||...||
  Fly  2872 SIRSCSSSSSSSAASAAAPSARRTS 2896

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Cacna1a	XP_006530650.1	Ion_trans	188..462	CDD:366146	100/297 (34%)
		Ion_trans	579..817	CDD:366146	71/241 (29%)
		PHA03247	<895..1277	CDD:223021	100/700 (14%)
		Ion_trans	1285..1520	CDD:366146	75/311 (24%)
		Ion_trans	1610..1869	CDD:366146	87/263 (33%)
		GPHH	1878..1931	CDD:374878	16/52 (31%)
		Ca_chan_IQ	1942..2015	CDD:370105	19/84 (23%)
		PHA03307	2046..>2460	CDD:223039	76/433 (18%)
NaCP60E	NP_001261172.1	Ion_trans	152..371	CDD:278921	78/220 (35%)
		Ion_trans	705..902	CDD:278921	58/204 (28%)
		Ion_trans	1768..2019	CDD:278921	82/271 (30%)
		Na_channel_gate	2009..2063	CDD:240441	11/57 (19%)
		Ion_trans	2085..2320	CDD:278921	80/245 (33%)
		GPHH	2331..2386	CDD:293510	19/59 (32%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG2301
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R175
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	4	3.840