DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment App and Appl

DIOPT Version :9

Sequence 1:	NP_001185752.1	Gene:	App / 11820	MGIID:	88059	Length:	770	Species:	Mus musculus
Sequence 2:	NP_001245448.1	Gene:	Appl / 31002	FlyBaseID:	FBgn0000108	Length:	890	Species:	Drosophila melanogaster

Alignment Length:	951	Identity:	239/951 - (25%)
Similarity:	380/951 - (39%)	Gaps:	263/951 - (27%)

- Green bases have known domain annotations that are detailed below.

Mouse     7 LLLLAAWTVRALEVPTDGNAGLLA-----EPQIAMFC--GKLNMHMNV-QNGKWESDPSGT---K 60
            |||.:.|.|.|:     |.|.:.|     |||||:.|  |::.....: :.|:|.:|.|..   .
  Fly     9 LLLRSLWVVLAI-----GTAQVQAASPRWEPQIAVLCEAGQIYQPQYLSEEGRWVTDLSKKTTGP 68

Mouse    61 TCIGTKEGILQYCQEVYPELQITNVVEANQPVTIQNWCKRG---RKQCKTHTHIVIPYRCLVGEF 122
            ||:..|..:|.||::.||...|||:||::....|..||::|   ..:||.....:.|:||| |.|
  Fly    69 TCLRDKMDLLDYCKKAYPNRDITNIVESSHYQKIGGWCRQGALNAAKCKGSHRWIKPFRCL-GPF 132

Mouse   123 VSDALLVPDKCKFLHQERMDVCETHLHWHTVAKETCSEKSTNLHDYGMLLPCGIDKFRGVEFVCC 187
            .|||||||:.|.|.|......|...:.|:......|.|:...:..:.|||||||..|.|||||||
  Fly   133 QSDALLVPEGCLFDHIHNASRCWPFVRWNQTGAAACQERGMQMRSFAMLLPCGISVFSGVEFVCC 197

Mouse   188 PLAEESD---------------SVDSADAE-----EDDSDVWWGGADTDYADGGEDKVVEVAEEE 232
            |...::|               .::||:.|     ||||:      |::|:
  Fly   198 PKHFKTDEIHVKKTDLPVMPAAQINSANDELVMNDEDDSN------DSNYS-------------- 242

Mouse   233 EVADVEEEEADDDEDVEDGDEVEEEAEEPYEEATE-RTTSTATTTTTTTESVEEVVREVCS-EQA 295
              .|..|::.||::|:...||.::...:  |.||. .:.:|.::..:.:.|::::..|..| |:.
  Fly   243 --KDANEDDLDDEDDLMGDDEEDDMVAD--EAATAGGSPNTGSSGDSNSGSLDDINAEYDSGEEG 303

Mouse   296 ETGPCRAMISRWYFDVTEGKCVPFFYGGCGGNRNNFDTEEYCMAVCGSVSTQSLLKTTSE----P 356
            :.                       |...|....:....|......|.....||...:|.    |
  Fly   304 DN-----------------------YEEDGAGSESEAEVEASWDQSGGAKVVSLKSDSSSPSSAP 345

Mouse   357 LPQDPDKLPT---------------------------TAASTPDAV-----DKYLETPGDENEHA 389
            :...|:|.|.                           |.|..|.:.     |.|........||.
  Fly   346 VAPAPEKAPVKSESVTSTPQLSASAAAFVAANSGNSGTGAGAPPSTAQPTSDPYFTHFDPHYEHQ 410

Mouse   390 HFQKAKERLEAKHRERMSQVMREWEEAERQAKNLPKADKKA-------VIQHFQEKVESLEQEAA 447
            .::.:::|||..|||::::||::|.:.|.:.:::..||.||       :...||..|::||:|..
  Fly   411 SYKVSQKRLEESHREKVTRVMKDWSDLEEKYQDMRLADPKAAQSFKQRMTARFQTSVQALEEEGN 475

Mouse   448 NERQQLVETHMARVEAMLNDRRRLALENYITALQAVPPRPHHVFNMLKKYVRAEQKDRQHTLKHF 512
            .|:.||...|..||.|.:|.|:|.|:..|..||...||..|||...|:|.:||..|||.|.|.|:
  Fly   476 AEKHQLAAMHQQRVLAHINQRKREAMTCYTQALTEQPPNAHHVEKCLQKLLRALHKDRAHALAHY 540

Mouse   513 EH-VRMVDP---KKAAQIRSQVMTHLRVIYERMNQSLSLLYNVPAVAEEI-------------QD 560
            .| :....|   :.||..|.:.:..|..|...:|||:::|...|.::.:|             :|
  Fly   541 RHLLNSGGPGGLEAAASERPRTLERLIDIDRAVNQSMTMLKRYPELSAKIAQLMNDYILALRSKD 605

Mouse   561 EV----------------------------------------------------DELLQKEQNYS 573
            ::                                                    :|.|:.|....
  Fly   606 DIPGSSLGMSEEAEAGILDKYRVEIERKVAEKERLRLAEKQRKEQRAAEREKLREEKLRLEAKKV 670

Mouse   574 DDVLANMISE-----------------------PRISYGNDALM-----PSLTETKTTVELLPVN 610
            ||:|.:.::|                       |....|.||.:     |:|..||:
  Fly   671 DDMLKSQVAEQQSQPTQSSTQSQAQQQQQEKSLPGKELGPDAALVTAANPNLETTKS-------- 727

Mouse   611 GEFSLDDLQPWHPFGVDSVPANTENEVEPVDARPAADRGLTTRPGSGLTNIKTEEISEVKMDAEF 675
             |..|.|.:    :|..:|....:..:..|| ..|..|.:.. ..:.:.:.:.|...:..|..:.
  Fly   728 -EKDLSDTE----YGEATVSTKVQTVLPTVD-DDAVQRAVED-VAAAVAHQEAEPQVQHFMTHDL 785

Mouse   676 GH-DSGFEVRHQKLVFFAEDVGSNK---GAIIGLMVGGVVIATVIVITLVMLKKKQYTSIH-HGV 735
            || :|.|.:|.:    ||:...:.|   .....|...|:.:...:.:.:.:.|.:...|.| .|.
  Fly   786 GHRESSFSLRRE----FAQHAHAAKEGRNVYFTLSFAGIALMAAVFVGVAVAKWRTSRSPHAQGF 846

Mouse   736 VEVDAA----------VTPEERHLSKMQQNGYENPTYKFFE 766
            :|||..          :..||:.:..||.|||||||||:||
  Fly   847 IEVDQVGCQNVTTHHPIVREEKIVPNMQINGYENPTYKYFE 887

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
App	NP_001185752.1	A4_EXTRA	24..188	CDD:128326	67/177 (38%)
		GFLD subdomain. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01217	28..123		36/108 (33%)
		Heparin-binding. /evidence=ECO:0000250\|UniProtKB:P05067	96..110		5/16 (31%)
		CuBD subdomain. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01217	131..189		22/57 (39%)
		Zinc-binding. /evidence=ECO:0000250	181..188		6/6 (100%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	193..284		22/111 (20%)
		Kunitz_BPTI	294..341	CDD:333766	3/46 (7%)
		OX-2. /evidence=ECO:0000250\|UniProtKB:P05067	344..365		6/24 (25%)
		APP_E2	366..548	CDD:372388	66/224 (29%)
		Heparin-binding. /evidence=ECO:0000250	391..423		10/31 (32%)
		Heparin-binding. /evidence=ECO:0000250	491..522		12/34 (35%)
		Collagen-binding. /evidence=ECO:0000250\|UniProtKB:P05067	523..540		5/16 (31%)
		Beta-APP	677..713	CDD:367525	9/39 (23%)
		Interaction with PSEN1. /evidence=ECO:0000250\|UniProtKB:P05067	695..722		3/29 (10%)
		APP_amyloid	716..766	CDD:371108	20/60 (33%)
		Basolateral sorting signal	724..734		3/10 (30%)
		Interaction with G(o)-alpha. /evidence=ECO:0000250	732..751		7/29 (24%)
		Interaction with DAB2. /evidence=ECO:0000269\|PubMed:11247302	756..770		10/11 (91%)
		Required for the interaction with KIF5B and for anterograde transport in axons. /evidence=ECO:0000250\|UniProtKB:P05067	756..770		10/11 (91%)
		YENPXY motif, contains endocytosis signal. /evidence=ECO:0000250\|UniProtKB:P05067	757..762		4/4 (100%)
Appl	NP_001245448.1	A4_EXTRA	26..198	CDD:128326	65/172 (38%)
		APP_N	33..141	CDD:280358	42/108 (39%)
		APP_Cu_bd	143..198	CDD:289676	21/54 (39%)
		APP_E2	387..580	CDD:289677	64/192 (33%)
		GBP_C	<629..682	CDD:303769	7/52 (13%)
		APP_amyloid	834..887	CDD:287486	20/52 (38%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167844132
Domainoid	1	1.000	108	1.000	Domainoid score	I6427
eggNOG	1	0.900	-	-		E1_KOG4295
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG50020
OrthoDB	1	1.010	-	-		D405630at33208
OrthoFinder	1	1.000	-	-		FOG0001588
OrthoInspector	1	1.000	-	-		otm43278
orthoMCL	1	0.900	-	-		OOG6_104222
Panther	1	1.100	-	-	O	PTHR23103
Phylome	0	0.000	Not matched by this tool.
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2378
SonicParanoid	1	1.000	-	-		X996
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	13	12.840