Protein Alignment Aplp1 and Appl

DIOPT Version :9

• Sequence 1: NP_031493.2
• Gene: Aplp1 / 11803
• MGIID: 88046
• Length: 654
• Species: Mus musculus
• Sequence 2: NP_001245448.1
• Gene: Appl / 31002
• FlyBaseID: FBgn0000108
• Length: 890
• Species: Drosophila melanogaster
• Alignment Length: 891
• Identity: 204/891 - (22%)
• Similarity: 314/891 - (35%)
• Gaps: 280/891 - (31%)

Mouse    29 LLLRAQLAVGNLAVGSP--SAAEAPGSAQVAGLC--GRLTLHRDL-RTGRWEPDPQRSR---RCL 85
            ||||:...|  ||:|:.  .||......|:|.||  |::...:.| ..|||..|..:..   .||
  Fly     9 LLLRSLWVV--LAIGTAQVQAASPRWEPQIAVLCEAGQIYQPQYLSEEGRWVTDLSKKTTGPTCL 71

Mouse    86 LDPQRVLEYCRQMYPELHIARVEQAAQAIPMERWC--GGTRSGRCAHPHHEVVPFHCLPGEFVSE 148
            .|...:|:||::.||...|..:.:::....:..||  |...:.:|...|..:.||.|| |.|.|:
  Fly    72 RDKMDLLDYCKKAYPNRDITNIVESSHYQKIGGWCRQGALNAAKCKGSHRWIKPFRCL-GPFQSD 135

Mouse   149 ALLVPEGCRFLHQERMDQCESSTRRHQEAQEACSSQGLILHGSGMLLPCGSDRFRGVEYVCCPP- 212
            ||||||||.|.|.....:|....|.:|....||..:|:.:....||||||...|.|||:||||.
  Fly   136 ALLVPEGCLFDHIHNASRCWPFVRWNQTGAAACQERGMQMRSFAMLLPCGISVFSGVEFVCCPKH 200

Mouse   213 -------------PATP------------------------------------------------ 216
                         |..|
  Fly   201 FKTDEIHVKKTDLPVMPAAQINSANDELVMNDEDDSNDSNYSKDANEDDLDDEDDLMGDDEEDDM 265

Mouse   217 --NPSGMAAGDPSTRSWPLGGRAEGGEDEEEVESFPQPVDDYFVEPPQAEEEEEEEE-------- 271
              :.:..|.|.|:|.|  .|....|..|:...|.......|.:.|.....|.|.|.|
  Fly   266 VADEAATAGGSPNTGS--SGDSNSGSLDDINAEYDSGEEGDNYEEDGAGSESEAEVEASWDQSGG 328

Mouse   272 -------------ERAP--PPSSHTPVMVSRVTPTP----------------------------R 293
                         ..||  |.....||....||.||                            :
  Fly   329 AKVVSLKSDSSSPSSAPVAPAPEKAPVKSESVTSTPQLSASAAAFVAANSGNSGTGAGAPPSTAQ 393

Mouse   294 PTDGVDVYFGMPGEIGEHEGFLRAKMDLEERRMRQINEVMREWAMADSQSKNLPKAD-------R 351
            ||.  |.||.......||:.:..::..|||....::..||::|:..:.:.:::..||       :
  Fly   394 PTS--DPYFTHFDPHYEHQSYKVSQKRLEESHREKVTRVMKDWSDLEEKYQDMRLADPKAAQSFK 456

Mouse   352 QALNEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQGDPPQAERVLMA 416
            |.:...||:.:|.|||:.:.|:.:|...|..||:|.||.::|.|:..:..||...||.|..|...
  Fly   457 QRMTARFQTSVQALEEEGNAEKHQLAAMHQQRVLAHINQRKREAMTCYTQALTEQPPNAHHVEKC 521

Mouse   417 LRRYLRAEQKEQRHTLRHYQHVA-AVDP---EKAQQMRFQVQTHLQVIEERMNQSLGLLDQNPHL 477
            |::.|||..|::.|.|.||:|:. :..|   |.|...|.:....|..|:..:|||:.:|.:.|.|
  Fly   522 LQKLLRALHKDRAHALAHYRHLLNSGGPGGLEAAASERPRTLERLIDIDRAVNQSMTMLKRYPEL 586

Mouse   478 AQELRPQIQELLLA---------EHLGPSE----------------------------------- 498
            :.::...:.:.:||         ..||.||
  Fly   587 SAKIAQLMNDYILALRSKDDIPGSSLGMSEEAEAGILDKYRVEIERKVAEKERLRLAEKQRKEQR 651

Mouse   499 -------------LDA---------------SVPGSSSEDKGSLQPPESKD------DPPVTLPK 529
                         |:|               |.|..||....:.|..:.|.      .|...|..
  Fly   652 AAEREKLREEKLRLEAKKVDDMLKSQVAEQQSQPTQSSTQSQAQQQQQEKSLPGKELGPDAALVT 716

Mouse   530 GSTDQESSSSGREKLTPLEQYEQKVNASAPRGFPFHSSD-IQR-----------DELAP------ 576
            .:.....::...:.|:..|..|..|:.......|....| :||           .|..|
  Fly   717 AANPNLETTKSEKDLSDTEYGEATVSTKVQTVLPTVDDDAVQRAVEDVAAAVAHQEAEPQVQHFM 781

Mouse   577 --------SGTGVSRE------------------ALSGLLIMGAGGGSLIVLSLLLLRKKKPYGT 615
                    |...:.||                  :.:|:.:|.|     :.:.:.:.:.:.....
  Fly   782 THDLGHRESSFSLRREFAQHAHAAKEGRNVYFTLSFAGIALMAA-----VFVGVAVAKWRTSRSP 841

Mouse   616 ISHGVVEVDPM----------LTLEEQQLRELQRHGYENPTYRFLE 651
            .:.|.:|||.:          :..||:.:..:|.:|||||||::.|
  Fly   842 HAQGFIEVDQVGCQNVTTHHPIVREEKIVPNMQINGYENPTYKYFE 887

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Aplp1	NP_031493.2	A4_EXTRA	46..211	CDD:128326	61/172 (35%)
		GFLD subdomain. /evidence=ECO:0000255|PROSITE-ProRule:PRU01217	50..146		29/103 (28%)
		APP_N	55..154	CDD:280358	35/106 (33%)
		CuBD subdomain. /evidence=ECO:0000255|PROSITE-ProRule:PRU01217	154..212		24/57 (42%)
		APP_Cu_bd	156..211	CDD:289676	21/54 (39%)
		Copper-binding	158..178		5/19 (26%)
		Zinc-binding. /evidence=ECO:0000250	204..211		5/6 (83%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	214..297		27/183 (15%)
		APP_E2	289..471	CDD:289677	60/220 (27%)
		Heparin-binding. /evidence=ECO:0000250	314..346		6/31 (19%)
		Heparin-binding. /evidence=ECO:0000250	414..445		11/34 (32%)
		Collagen-binding. /evidence=ECO:0000250	446..463		4/16 (25%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	497..580		23/177 (13%)
		Basolateral sorting signal. /evidence=ECO:0000250	608..619		0/10 (0%)
		APP_amyloid	<612..651	CDD:287486	14/48 (29%)
		Interaction with DAB1	636..652		9/16 (56%)
		Interaction with DAB2. /evidence=ECO:0000269|PubMed:11247302	640..654		8/12 (67%)
		NPXY motif, contains endocytosis signal	644..647		2/2 (100%)
Appl	NP_001245448.1	A4_EXTRA	26..198	CDD:128326	61/172 (35%)
		APP_N	33..141	CDD:280358	35/108 (32%)
		APP_Cu_bd	143..198	CDD:289676	21/54 (39%)
		APP_E2	387..580	CDD:289677	57/194 (29%)
		GBP_C	<629..682	CDD:303769	2/52 (4%)
		APP_amyloid	834..887	CDD:287486	14/52 (27%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167844134
Domainoid	1	1.000	108	1.000	Domainoid score	I6427
eggNOG	1	0.900	-	-		E1_KOG4295
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	245	1.000	Inparanoid score	I3265
Isobase	1	0.950	-	0	Normalized mean entropy	S7440
OMA	1	1.010	-	-		QHG50020
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0001588
OrthoInspector	1	1.000	-	-		otm43278
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	LDO	PTHR23103
Phylome	0	0.000	Not matched by this tool.
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2378
SonicParanoid	1	1.000	-	-		X996
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	13	12.930