Protein Alignment mitfb and Mitf

DIOPT Version :9

• Sequence 1: NP_571922.2
• Gene: mitfb / 114833
• ZFINID: ZDB-GENE-010919-1
• Length: 500
• Species: Danio rerio
• Sequence 2: NP_001245436.1
• Gene: Mitf / 3885647
• FlyBaseID: FBgn0263112
• Length: 837
• Species: Drosophila melanogaster
• Alignment Length: 670
• Identity: 179/670 - (26%)
• Similarity: 266/670 - (39%)
• Gaps: 250/670 - (37%)

Zfish    47 PPLG----------SSRVLLRQQLMREQLQQQERREQQKRQISITHSPAINV------------- 88
            |||.          :||..|:.||.||| |||...:||  .:.....|.:::
  Fly   163 PPLSGGPTFKTATPTSRTQLKLQLQREQ-QQQMMIQQQ--TLDTAMDPKMHLLFGSGQGLMESEF 224

Zfish    89 -----SHPCGPPSAA-------------------------------QVPMEVLKVQTHLENPTKY 117
                 :..||..|::                               .||.:||:|.|.|||||:|
  Fly   225 IDSGSTSACGSGSSSLEQMSQLVQMDNLIDSSSGAKLKVPLQSIGVDVPPQVLQVSTVLENPTRY 289

Zfish   118 HIQQAQRQQVKAYLSTTLGGKQAVSLPCPSQASDHGGMPPGPGNSAPNSPMALLTLNPNCEKEMD 182
            |:.|.|:.||:.|||.:..         ||....|........|||....:.    |.:.:|.:.
  Fly   290 HVIQKQKNQVRQYLSESFK---------PSMWGSHTSEIKLANNSASTGNLQ----NSSLQKGIC 341

Zfish   183 DVID--DIIGLESSY-------SDDIMGLS--------------LDP------------------ 206
            |.::  :..|.:|:.       |||.|.:|              ::|
  Fly   342 DPLERTNRFGCDSAVSAKRIMPSDDAMPISPFGGSFVRCDDINPIEPTVLRPNSHGAGEPENAHR 406

Zfish   207 ----GLQMANTIPVSANLLDMYSNPGLPPPGISISNSCPANMTSVKREFSVTP-SPALMHM---M 263
                ||..||     ::|....|:.|       |.||...:.||...:.:..| ||::..:   :
  Fly   407 TAQLGLSKAN-----SSLSSTRSSSG-------IVNSIRISSTSSSLQSTSAPISPSVSSVATSV 459

Zfish   264 DKPSSC-----------------SKFESYQRPEGFPVEVEVRALAKERQKKDNHNLIERRRRFNI 311
            .:|...                 |:....|.|:.. .:.|:.||||:||||||||:|||||||||
  Fly   460 SEPDDIFDDILQNDSFNFDKNFNSELSIKQEPQNL-TDAEMNALAKDRQKKDNHNMIERRRRFNI 523

Zfish   312 NDRIKELGTLIPKSNDP------DMRWNKGTILKASVDYIRKLQREQHRTKELENRQKKLEHTNR 370
            ||||||||||:||.:|.      |:|.|||||||:|||||:.|:.|..|.::.|.||:::|..||
  Fly   524 NDRIKELGTLLPKGSDAFYEVVRDIRPNKGTILKSSVDYIKCLKHEVTRLRQNELRQRQVELQNR 588

Zfish   371 NLLLRIQELEIQARAHGLAMASSALCA-----------------SELAARAIKQEPL-------- 410
            .|:.||:|||:||::||:.::.:.|.:                 :..:.|::..:|:
  Fly   589 KLMSRIKELEMQAKSHGILLSENHLTSLSAPTQPYLKSFSLSPTASRSRRSLFDQPVEKKIQVID 653

Zfish   411 --------------LGDCSQ-----DMYTSTHAHPSCADMSRSSTLDLNNGTISFSDTHLSDTHA 456
                          :.||..     |...|:|:|...|..|.||. .||:|  ||...:.|:...
  Fly   654 GADGNMGMNQVDEFMEDCKYAVQGGDPMLSSHSHMQSAPQSPSSK-TLNSG--SFEPKNFSEADE 715

Zfish   457 AKL--------DD---------ILMDETLTS--GATN----------ESLISAASN--------- 483
            :..        ||         ..:...|||  |..|          .||..:|.|
  Fly   716 SLFRGKSSLASDDCCGINCSTSCYIQHQLTSREGHPNHSHHTGIREIHSLSDSAQNSEFSRLEHC 780

Zfish   484 -----ESTHKDSMNMEENQH 498
                 .|:|:....::|:||
  Fly   781 DDPLLSSSHRSLGTVDEDQH 800

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
mitfb	NP_571922.2	MITF_TFEB_C_3_N	53..185	CDD:292573	45/180 (25%)
		HLH	293..353	CDD:238036	46/65 (71%)
		DUF3847	347..>380	CDD:289710	13/32 (41%)
		DUF3371	381..496	CDD:288684	36/201 (18%)
Mitf	NP_001245436.1	MITF_TFEB_C_3_N	<272..>311	CDD:292573	21/47 (45%)
		HLH	505..570	CDD:238036	45/64 (70%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170593387
Domainoid	1	1.000	85	1.000	Domainoid score	I8110
eggNOG	1	0.900	-	-		E1_KOG1318
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	150	1.000	Inparanoid score	I4340
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D1211990at2759
OrthoFinder	1	1.000	-	-		FOG0000691
OrthoInspector	1	1.000	-	-		otm24979
orthoMCL	1	0.900	-	-		OOG6_109347
Panther	1	1.100	-	-	O	PTHR45776
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1708
SonicParanoid	1	1.000	-	-		X566
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	14	13.790