Protein Alignment CHRM1 and mAChR-B

DIOPT Version :10

• Sequence 1: NP_000729.2
• Gene: CHRM1 / 1128
• HGNCID: 1950
• Length: 460
• Species: Homo sapiens
• Sequence 2: NP_649764.1
• Gene: mAChR-B / 40955
• FlyBaseID: FBgn0037546
• Length: 1019
• Species: Drosophila melanogaster
• Alignment Length: 925
• Identity: 174/925 - (18%)
• Similarity: 258/925 - (27%)
• Gaps: 499/925 - (53%)

Human    10 SPNITVLAPGKGP---WQVAFIGITTGLLSLATVTGNLLVLISFKVNTELKTVNNYFLLSLACAD 71
            |.:..:|.|...|   ||...|.|...:..:.||.||:|||::|.|:..::..:|||:.|||..|
  Fly   115 SSDSEILGPVLPPFALWQTILIAICLAICIILTVGGNILVLLAFIVDRNIRQPSNYFIASLAATD 179

Human    72 LIIGTFSMNLYTTYLLMGHWALGTLACDLWLALDYVASNASVMNLLLISFDRYFSVTRPLSYRAK 136
            ::|||.||..||.|:|.|:|.||.:.|||||::||.....|...:|||:.|||.||.....||:.
  Fly   180 MLIGTVSMPFYTIYVLKGYWDLGPMLCDLWLSVDYTVCLVSQYTVLLITIDRYCSVKIAAKYRSW 244

Human   137 RTPRRAALMIGLAWLVSFVLWAPAILFWQYLVGERTVLAGQCYIQFLSQPIITFGTAMAAFYLPV 201
            ||..|...|:.:.|::..:|:..:|..|::..|:|.:|.|||.:|||..||......:..::..:
  Fly   245 RTRTRVIYMVTITWIIPALLFFISIFGWEHFTGKRDLLPGQCAVQFLKDPIFNTALIIGYYWTTL 309

Human   202 TVMCTLYWRIYR----------------------------------------------------- 213
            .|:..||..||:
  Fly   310 IVLFVLYAGIYKTAYDMQKRSEAKQRKMQSMVALSAGAMSGMAGHAAGIGVIEEKILKTKVELAG 374

Human   214 ------------------------------ETE-------------------------------- 216
                                          |.|
  Fly   375 DQTDLDSACTTVIKRLSGSGQANPLAVATEEVEKMTPEQRRASAAKIQEEAKKREAAVDAEKSER 439

Human   217 --------------NRARELAALQ---------------GSE-----TPGKG------------- 234
                          |:|::|...|               |::     |.|||
  Fly   440 SSSPAFDSDEESSVNQAQQLITQQKLNNMRKRSSIGLVFGAQAALLATRGKGNLQKSTTNSKSIE 504

Human   235 ----------------------------------------------------------------- 234
                                                                             
  Fly   505 AMHQYHHHQQHHHNQSPLQRAQSKEEMRSLHHHQNQQQPHHQHHQNQPNPPLDRPKRTSCSTLSQ 569

Human   235 ----------------------------------------------------------------- 234
                                                                             
  Fly   570 IAEHDRLVDLSAPPTLLNTSDPLSPVDLAPIEVPSDQVHQGLVQTILPPPDAFQCPTPLSDDYSD 634

Human   235 ---GGSSSSSERS--------------------------------QPGAEGSPET----PPGRCC 260
               |.||.:||.:                                ....:|.|..    ||.|
  Fly   635 RPFGNSSGNSELALTYDLMTNSELRYMDESSAMLASVTANSTTSPNDSVQGKPPVLPPPPPAR-- 697

Human   261 RCCRAP-----------------------------------------------RLLQAYSWKEE- 277
               |.|                                               |||.:|:..|:
  Fly   698 ---RNPPKSNLNQSLSQTPSQNQSPSQSLSLNLNPNHNQSQSQSIEEAVAIEKRLLVSYTGIEDF 759

Human   278 -EEEDEGSMESL-----------------TSSEGEEPGSEVVIKMPM----VDPEAQAPTKQ--- 317
             :...|..:|::                 .||..|....:.::..||    :.|:.:..|.|
  Fly   760 AKVRRESCIEAICLLDVPGKLAADCPHRRASSPMETSSKDAILYSPMPPMPLSPKVKTSTPQTGT 824

Human   318 --------PP----------RSSPNTVKRP-----------------------TKK--GRDRAGK 339
                    ||          .|..||.|.|                       |||  |.|.|.:
  Fly   825 PQSAAGVTPPVPLERIEERETSDSNTNKMPSTSGTTSSTGGVGGGGEQDGVGATKKNAGDDDANR 889

Human   340 GQKP--------------------RGKEQL-------AKRKTFSLVKEKKAARTLSAILLAFILT 377
            .:|.                    :.|:.|       .::|:.|..:.:||.||:|.||..|:..
  Fly   890 EEKSLAASRNSSKRAFIHSIGKHFKSKKALPLILGVGGRQKSKSENRARKAFRTISFILGCFVAC 954

Human   378 WTPYNIMVLVSTFCK--DCVPETLWELGYWLCYVNSTINPMCYALCNKAFRDTFRLLLLCRWDKR 440
            ||||:::.||..||:  .|:.|.|:...|:|||.||.:||.||||.|:.|:.||..:|
  Fly   955 WTPYHVLALVEGFCRHPPCINEHLYMFSYFLCYANSPMNPFCYALANQQFKKTFTRIL------- 1012

Human   441 RWRKIPKRPGSVHRT 455
                    .|.:|.|
  Fly  1013 --------KGDLHMT 1019

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
CHRM1	NP_000729.2	7tmA_mAChR_M1	26..429	CDD:341356	162/878 (18%)
		TM helix 1	27..53	CDD:341356	10/25 (40%)
		TM helix 2	60..86	CDD:341356	14/25 (56%)
		TM helix 3	98..128	CDD:341356	15/29 (52%)
		TM helix 4	140..163	CDD:341356	5/22 (23%)
		TM helix 5	185..214	CDD:341356	7/111 (6%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	225..256		13/232 (6%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	274..297		6/41 (15%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	310..351		18/113 (16%)
		TM helix 6	358..388	CDD:341356	13/29 (45%)
		TM helix 7	397..422	CDD:341356	14/24 (58%)
mAChR-B	NP_649764.1	7tmA_mAChR_GAR-2-like	147..>323	CDD:320429	71/175 (41%)
		TM helix 2	168..194	CDD:320429	14/25 (56%)
		TM helix 3	206..236	CDD:320429	15/29 (52%)
		TM helix 4	248..271	CDD:320429	5/22 (23%)
		TM helix 5	293..322	CDD:320429	7/28 (25%)
		7tm_GPCRs	<932..1008	CDD:475119	34/75 (45%)
		TM helix 6	941..963	CDD:410628	10/21 (48%)
		TM helix 7	976..1001	CDD:410628	14/24 (58%)