Protein Alignment CFTR and VMR1

DIOPT Version :10

• Sequence 1: NP_000483.3
• Gene: CFTR / 1080
• HGNCID: 1884
• Length: 1480
• Species: Homo sapiens
• Sequence 2: NP_011828.1
• Gene: VMR1 / 856350
• SGDID: S000001027
• Length: 1592
• Species: Saccharomyces cerevisiae
• Alignment Length: 1536
• Identity: 391/1536 - (25%)
• Similarity: 692/1536 - (45%)
• Gaps: 305/1536 - (19%)

Human     9 ASVVSKLFFSWTRPILRKGYRQRLELSDIYQIPSVDSADNLSEKLEREWDRELASKKNPKLINAL 73
            :|::|.:.:||....:.:..:..::|.||:.:...|.:..:.:...|         :| |.||.|
Yeast   272 SSILSCITWSWITNFIWEAQKNTIKLKDIWGLSMEDYSIFILKGFTR---------RN-KHINNL 326

Human    74 RRCFFWRFMFY----GIFLYLGEVTKAVQPLLLGRIIASYDPDNKEERSIAI----YLGIGLCLL 130
            ....|..|..|    .:::.:..:...:..:|:.|.:...|..|:....:.:    .:|:.:|.|
Yeast   327 TLALFESFKTYLLIGMLWVLVNSIVNLLPTILMKRFLEIVDNPNRSSSCMNLAWLYIIGMFICRL 391

Human   131 FIVRTLLL--HPAIFGLHHIGMQMRIAMFSLIYKKTLK-------LSSRVLDKIS--IGQLVSLL 184
                ||.:  ....|....|.:::|..:...||.|.|:       .:|...|.||  :|.:::|:
Yeast   392 ----TLAICNSQGQFVSDKICLRIRAILIGEIYAKGLRRRLFTSPKTSSDSDSISANLGTIINLI 452

Human   185 SNNLNKFDEGLALAHFVWI---APLQVALLMGLIWELLQASAFCGLGFLIVLALFQAGLGRMMMK 246
            |.:..|..|   ||:::::   |.:.:.:::||::..|..|||.|:..::|:......|..::.|
Yeast   453 SIDSFKVSE---LANYLYVTVQAVIMIIVVVGLLFNFLGVSAFAGISIILVMFPLNFLLANLLGK 514

Human   247 YRDQRAGKISERLVITSEMIENIQSVKAYCWEEAMEKMIENLRQTELKLTRKAAYVRYFNSSAFF 311
            ::.|......:|:...:|.::||:.||.:.||..:...|:::||.||:...|.:.|....|..:|
Yeast   515 FQKQTLKCTDQRISKLNECLQNIRIVKYFAWERNIINEIKSIRQKELRSLLKKSLVWSVTSFLWF 579

Human   312 FSGFFV--VFLSVLPYALIKGIILRKIFTTISFCIVLRMAVTRQFPWAVQTWYDSLGAINKIQDF 374
            .:...|  |..::..:...:.:.....|||:|...:|:..:. |....:.....|..::.:|.||
Yeast   580 VTPTLVTGVTFAICTFVQHEDLNAPLAFTTLSLFTLLKTPLD-QLSNMLSFINQSKVSLKRISDF 643

Human   375 LQKQEYKTLEYNLTT-----TEVVMENVTAFWEEGFGELFEKAKQNNNNRKTSNGDDSLFFSNFS 434
            |:..:  |.:||..|     .::..:|.|..|.|                   |..|   .:.|.
Yeast   644 LRMDD--TEKYNQLTISPDKNKIEFKNATLTWNE-------------------NDSD---MNAFK 684

Human   435 LLGTPVLKDINFKIERGQLLAVAGSTGAGKTSLLMVIMGELEPSEGKI-------KH-------- 484
            |.|      :|.|.:.|:|..:.||||:||::||:.::|||....|.|       ||
Yeast   685 LCG------LNIKFQIGKLNLILGSTGSGKSALLLGLLGELNLISGSIIVPSLEPKHDLIPDCEG 743

Human   485 -SGRISFCSQFSWIMPGTIKENIIFGVSYDEYRYRSVIKACQLEEDISKFAEKDNIVLGEGGITL 548
             :...::|||.:|::..|:|.||||...|:|.||..||.||.|:.|:......|...:||.||||
Yeast   744 LTNSFAYCSQSAWLLNDTVKNNIIFDNFYNEDRYNKVIDACGLKRDLEILPAGDLTEIGEKGITL 808

Human   549 SGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEKEIFESCVC-KLMANKTRILVTSKME-HLK 611
            ||||:.|||||||||..|...|||.....:|..|...|:|:|:. .||.|:|.||||..:. .|:
Yeast   809 SGGQKQRISLARAVYSSAKHVLLDDCLSAVDSHTAVWIYENCITGPLMKNRTCILVTHNVSLTLR 873

Human   612 KADKILILHEGSSYFYGTFSELQNLQPDFSSKLMGCDSFDQFSAERRNSILTETLHRFSLEGDAP 676
            .|...::|..|.....||.:|||       ||.:..:.:.|.|:  |:||..:..:|..    ||
Yeast   874 NAHFAIVLENGKVKNQGTITELQ-------SKGLFKEKYVQLSS--RDSINEKNANRLK----AP 925

Human   677 VSWTETKKQSFKQTGEFGEKRKNSILNPINSIRKFSIVQKTPLQMNGIEEDSDEPLERRLSLVPD 741
                                |||                                         |
Yeast   926 --------------------RKN-----------------------------------------D 929

Human   742 SEQGEAILPRISVISTGPTLQARRRQSVLNLMTHSVNQGQNIHRKTTASTRKVSLAPQANLTELD 806
            |::.|.:...|                  |...:.||.||.|..:..::.   :::|       |
Yeast   930 SQKIEPVTENI------------------NFDANFVNDGQLIEEEEKSNG---AISP-------D 966

Human   807 IYSRRLSQETGLEISEEINEEDLKECFFDDMESIPAVTTWNTYLRYITVHKSLIFVLIWCLVIFL 871
            :|...|.                   ||...:::.|:     :..|||..           ::|:
Yeast   967 VYKWYLK-------------------FFGGFKALTAL-----FALYITAQ-----------ILFI 996

Human   872 AEVAASLVVLWL-------------LGNTPLQDKGNSTHSRNNSYAVIITSTSSYYV-FYIYVGV 922
            ::  :..:..|:             :...||:...:|:.:::|::         ||: .|..:|:
Yeast   997 SQ--SWWIRHWVNDTNVRINAPGFAMDTLPLKGMTDSSKNKHNAF---------YYLTVYFLIGI 1050

Human   923 ADTLLAMGFFRGLPLVHTLITVSKILHHKMLHSVLQAPMSTLNTLKAGGILNRFSKDIAILD-DL 986
            ...:|  |.|:.:....:.:..|:.:.:.:|..||.|.:...:....|.|:|||||||..:| :|
Yeast  1051 IQAML--GGFKTMMTFLSGMRASRKIFNNLLDLVLHAQIRFFDVTPVGRIMNRFSKDIEGVDQEL 1113

Human   987 LP---LTIFDFIQLLLIVIGAIAVVAVLQPYIFVATVPVIVAFIMLRAYFLQTSQQLKQLESEGR 1048
            :|   :|||..||...|:.    ::.|:.|......|.|.|.:..:..::|..|::||:|:|..:
Yeast  1114 IPYLEVTIFCLIQCASIIF----LITVITPRFLTVAVIVFVLYFFVGKWYLTASRELKRLDSITK 1174

Human  1049 SPIFTHLVTSLKGLWTLRAFGRQPYFETLFHKALNLHTAN-WFLYLS-TLRWFQMRIEMIFVIFF 1111
            ||||.|...:|.|:.|:||||.:..|  :......:...| .|.||| |::||..|::||.....
Yeast  1175 SPIFQHFSETLVGVCTIRAFGDERRF--ILENMNKIDQNNRAFFYLSVTVKWFSFRVDMIGAFIV 1237

Human  1112 IAV-TFISILTTGEGEGRVGIILTLAMNIMSTLQWAVNSSIDVDSLMRSVSRVFKFIDMPTEGKP 1175
            :|. :||.:.......|..||.||.|:.......|.|......:..|.||.|:.::..:..|.
Yeast  1238 LASGSFILLNIANIDSGLAGISLTYAILFTDGALWLVRLYSTFEMNMNSVERLKEYSSIEQEN-- 1300

Human  1176 TKSTKPYKNGQLSKVMIIENSHVKKDDIWPSGGQMTVKDLTAKYTEGGNAILENISFSISPGQRV 1240
                  |......:::::      .:..||..|::.:::|:.:|......::.|:||.:.|..::
Yeast  1301 ------YLGHDEGRILLL------NEPSWPKDGEIEIENLSLRYAPNLPPVIRNVSFKVDPQSKI 1353

Human  1241 GLLGRTGSGKSTLLSAFLRLLN-TEGEIQIDGVSWDSITLQQWRKAFGVIPQKVFIFSGTFRKNL 1304
            |::||||:||||:::|..|||. ..|.|:|||.....|.|...|::..:|||...:|:||.:.|:
Yeast  1354 GIVGRTGAGKSTIITALFRLLEPITGCIKIDGQDISKIDLVTLRRSITIIPQDPILFAGTIKSNV 1418

Human  1305 DPYEQWSDQEIWKVADEVGLRS------VI---EQFPG------KLDFVLVDGGCVLSHGHKQLM 1354
            |||:::.:::|:|...:|.|.|      |:   |:|..      .|...:.:||..||.|.:||:
Yeast  1419 DPYDEYDEKKIFKALSQVNLISSHEFEEVLNSEERFNSTHNKFLNLHTEIAEGGLNLSQGERQLL 1483

Human  1355 CLARSVLSKAKILLLDEPSAHLDPVTYQIIRRTLKQAFADCTVILCEHRIEAMLECQQFLVIEEN 1419
            .:|||:|.:.||:||||.::.:|..:..:|:..::..|...|::...||:.::::..:.:|::..
Yeast  1484 FIARSLLREPKIILLDEATSSIDYDSDHLIQGIIRSEFNKSTILTIAHRLRSVIDYDRIIVMDAG 1548

Human  1420 KVRQYDSIQKLL-NERSLFRQAISPSDRVKLFPH--RNSSK 1457
            :|::||...:|| :||.:|......|..::|...  :.|||
Yeast  1549 EVKEYDRPSELLKDERGIFYSMCRDSGGLELLKQIAKQSSK 1589

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
CFTR	NP_000483.3	CFTR_protein	1..1480	CDD:273530	391/1536 (25%)
		Disordered R region. /evidence=ECO:0000269|PubMed:10792060	654..831		24/176 (14%)
		Interaction with GORASP2. /evidence=ECO:0000269|PubMed:21884936	1386..1480		18/75 (24%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1452..1480		3/8 (38%)
		PDZ-binding. /evidence=ECO:0000269|PubMed:11707463, ECO:0000269|PubMed:16331976	1478..1480
VMR1	NP_011828.1	MRP_assoc_pro	212..1575	CDD:188098	386/1520 (25%)