Protein Alignment CFTR and YBT1

DIOPT Version :10

• Sequence 1: NP_000483.3
• Gene: CFTR / 1080
• HGNCID: 1884
• Length: 1480
• Species: Homo sapiens
• Sequence 2: NP_013052.1
• Gene: YBT1 / 850678
• SGDID: S000003971
• Length: 1661
• Species: Saccharomyces cerevisiae
• Alignment Length: 1569
• Identity: 399/1569 - (25%)
• Similarity: 696/1569 - (44%)
• Gaps: 303/1569 - (19%)

Human     4 SPLEKASVVSKLFFSWTRPILRKGYRQRLELSDIYQIPSVDSADNLSEKLEREWDRELASKKNPK 68
            ||....|:...:.::|....:.|.::..:::.||:.:...|.:..:.:|.....|.::..|:
Yeast   279 SPEPVTSIAGFICWAWLDSFVWKAHKVSIKVKDIWGLMMQDYSFFVVKKFRYFVDHKVKRKR--- 340

Human    69 LINALRRCFFWR--FMFYGIFLYLGEVTKAVQPLLLGRIIASYDPDNKEERSIAIYLGIGLCLLF 131
             |.:|...||:.  .:....:.:||.|...:..:||.||:...:..:....::|.:.   :.::|
Yeast   341 -IFSLNLFFFFSNYLVLQCFWAFLGSVLSFIPTVLLKRILEYVEDQSSAPSNLAWFY---VTVMF 401

Human   132 IVRTLLL---HPAIFGLHHIGMQMRIAMFSLIYKKTL--KLSS-----------RVLDKISI--- 177
            :.|.|:.   ..|:|....:.::|:..:.|.||.|.|  |:|:           .:.|:.||
Yeast   402 VGRILVAICQAQALFFGRRVCIRMKSIIISEIYTKALRRKISTNKTKPSNEDPQEINDQKSINGD 466

Human   178 ---------GQLVSLLSNNLNKFDEGLALAHFVWIAPLQVALLMGLIWELLQASAFCGLGFLIVL 233
                     |.:::|::.:..|..|.....|....|.:...:.:.|::.||..:|.  :|.||::
Yeast   467 EESTSSANLGAIINLMAIDAFKVSEICGYLHSFLEAFVMTVVALALLYRLLGFAAI--VGVLIIV 529

Human   234 ALFQAGLGRMMMKY----RDQRAGKISERLVITSEMIENIQSVKAYCWEEAMEKMIENLRQTELK 294
            |:..  |...:.||    :.:.......|:...:|..:.|:.:|.:.|||..||.|..:|:.||.
Yeast   530 AMLP--LNYKLAKYIGDLQKKNLAVTDNRIQKLNEAFQAIRIIKYFSWEENFEKDINTIRENELS 592

Human   295 LTRKAAYVRYFNSSAFFFSGFFVVFLSVLPYALIKGIILRK--IFTTISFCIVLRMAVTR---QF 354
            |....:.|...:|..:|.:...|...|...|..::|.:|..  .||.:|...:||..:.|   ..
Yeast   593 LLLMRSIVWSISSFLWFVTPTIVTAASFAYYIYVQGEVLTTPVAFTALSLFTLLRDPLDRLSDML 657

Human   355 PWAVQTWYDSLGAINKIQDFLQKQEYKTLEYNLTTTE-----VVMENVTAFWEEGFGELFEKAKQ 414
            .:.||    |..:::::||||.:.:  |.:|:..|.:     ...||.|..|:           :
Yeast   658 SFVVQ----SKVSLDRVQDFLNEND--TKKYDQLTIDPNGNRFAFENSTISWD-----------K 705

Human   415 NNNNRKTSNGDDSLFFSNFSLLGTPVLKDINFKIERGQLLAVAGSTGAGKTSLLMVIMGELEPSE 479
            :|.:.|                    |||:|.:.:.|:|..|.|.||:|||||||.::||:....
Yeast   706 DNQDFK--------------------LKDLNIEFKTGKLNVVIGPTGSGKTSLLMALLGEMYLLN 750

Human   480 GKI----------------KHSGRISFCSQFSWIMPGTIKENIIFGVSYDEYRYRSVIKACQLEE 528
            ||:                ..:..|::|||.:|::..|:|.||:|...::|.||::|::||.|:.
Yeast   751 GKVVVPALEPRQELIVDANGTTNSIAYCSQAAWLLNDTVKNNILFNSPFNEARYKAVVEACGLKR 815

Human   529 DISKFAEKDNIVLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEKEIFESCVC- 592
            |.......|...:||.||||||||:.|:|||||:|.:|...|||.....:|..|...|:::|:.
Yeast   816 DFEILKAGDLTEIGEKGITLSGGQKQRVSLARALYSNARHVLLDDCLSAVDSHTASWIYDNCITG 880

Human   593 KLMANKTRILVTSKME-HLKKADKILILHEGSSYFYGTFSELQNLQPDFSSKLMGCDSFDQFSAE 656
            .||.::|.|||:..:. .|:.|:.:::|.:|
Yeast   881 PLMEDRTCILVSHNIALTLRNAELVVLLEDG---------------------------------- 911

Human   657 RRNSILTETLHRFSLEGDAPVSWTETKKQSFKQTGEFGEKR--KNSILNPINSIRKFSIVQKTPL 719
                       |...:|| |:        ...|.|.|||..  |:|||:..||....:....|.|
Yeast   912 -----------RVKDQGD-PI--------DMLQKGLFGEDELVKSSILSRANSSANLAAKSSTSL 956

Human   720 QMNGIEEDSDEPLERRLSLVPDSEQGEAILPRISVISTGPTLQARRRQSVLNLMTHSVNQGQNIH 784
            .                :|....||      ::||.:.....:|::.|..|.........|:.|.
Yeast   957 S----------------NLPAVKEQ------QVSVNNNSSHFEAKKLQKSLRTEAERTEDGKLIK 999

Human   785 RKTTASTRKVSLAPQANLTELDIYSRRLSQETGLEISEEINEEDLKECFFDDMESIPAV----TT 845
            .:|          .:..:..||:|...|....|.:|..          |...:..|..:    .:
Yeast  1000 EET----------KEEGVVGLDVYKWYLKIFGGWKIVS----------FLASLFLIAQLLYIGQS 1044

Human   846 WNTYLRYITVHKSLIFVL--IWCLVIFLAEVAASLVVLWLLGNTPL-----QDKGNSTHSRNNSY 903
            |  ::|....|..:..::  ....:.|:::.|:.|:. | .|::.:     :::.:|.|
Yeast  1045 W--WVRAWASHNVIAKIIPRAQRAIAFISKKASHLID-W-RGSSQISMASAENQPSSGH------ 1099

Human   904 AVIITSTSSYYVFYIYVGVADTLLAMG-----FFRGLPLVHTLITVSKILHHKMLHSVLQAPMST 963
                 ||..|.|.|:.:|.|..||..|     |..|       |..|:.:.:.:|:.||.:.:..
Yeast  1100 -----STMYYLVLYLIIGFAQALLGAGKTILNFVAG-------INASRKIFNMILNKVLHSKIRF 1152

Human   964 LNTLKAGGILNRFSKDIAILDDLLPLTIFDFIQLLLIVIGAIAVVAVLQPYIFVATVPVIVAFIM 1028
            .:....|.|:|||||||..:|..|...|......|:..:..:.::..:.|......:.|.:.:..
Yeast  1153 FDATPTGRIMNRFSKDIEAIDQELTPYIQGAFYSLIECLSTVILITFITPQFLSVAIVVSILYYF 1217

Human  1029 LRAYFLQTSQQLKQLESEGRSPIFTHLVTSLKGLWTLRAFGRQPYF--ETLFHKALNLHTANWFL 1091
            :..:::..|::||:.||..||||:.|...:|.|:.|:||||.:..|  |.| ||....:...::|
Yeast  1218 VGYFYMAGSRELKRFESISRSPIYQHFSETLVGVTTIRAFGDEGRFMQENL-HKIDENNKPFFYL 1281

Human  1092 YLSTLRWFQMRIEMI-FVIFFIAVTFISILTTGEGEGRVGIILTLAMNIMSTLQWAVNSSIDVDS 1155
            :::. ||...||:|| .::.|.|..||.........|..||.||.|::......|.|....:|:.
Yeast  1282 WVAN-RWLAFRIDMIGSLVIFGAGLFILFNINNLDSGMAGISLTYAISFTEGALWLVRLYSEVEM 1345

Human  1156 LMRSVSRVFKFIDMPTEGKPTKSTKPYKNGQLSKVMIIENSHVK-KDDIWPSGGQMTVKDLTAKY 1219
            .|.||.||.:::::..|        ||            |.|.: ....||..|::.|.||:.:|
Yeast  1346 NMNSVERVKEYMEIEQE--------PY------------NEHKEIPPPQWPQDGKIEVNDLSLRY 1390

Human  1220 TEGGNAILENISFSISPGQRVGLLGRTGSGKSTLLSAFLRLLNTE-GEIQIDGVSWDSITLQQWR 1283
            ......:::|:|||:....::|::||||:||||:::|..|.|..| |.|:||.:....:.||:.|
Yeast  1391 APNLPRVIKNVSFSVDAQSKIGIVGRTGAGKSTIITALFRFLEPETGHIKIDNIDISGVDLQRLR 1455

Human  1284 KAFGVIPQKVFIFSGTFRKNLDPYEQWSDQEIWKVADEVGLRSVIEQFPG--------------- 1333
            ::..:|||...:||||.:.|||||:::||::|::....|.|.|..:...|
Yeast  1456 RSITIIPQDPTLFSGTIKTNLDPYDEFSDRQIFEALKRVNLISEEQLQQGATRETSNEASSTNSE 1520

Human  1334 ------KLDFVLVDGGCVLSHGHKQLMCLARSVLSKAKILLLDEPSAHLDPVTYQIIRRTLKQAF 1392
                  .|...:.:||..||.|.:||||||||:|...||:||||.:|.:|..:...|:.|:::.|
Yeast  1521 NVNKFLDLSSEISEGGSNLSQGQRQLMCLARSLLRSPKIILLDEATASIDYSSDAKIQETIRKEF 1585

Human  1393 ADCTVILCEHRIEAMLECQQFLVIEENKVRQYD-SIQKLLNERSLFRQAISPSDRV--------K 1448
            ...|::...||:.::::..:.||::..:|::|| ....|||::|.|......|..:        |
Yeast  1586 QGSTILTIAHRLRSVIDYDKILVMDAGEVKEYDHPYSLLLNKQSAFYSMCEHSGELDILIELAKK 1650

Human  1449 LFPHRNSSK 1457
            .|..:.:||
Yeast  1651 AFVEKLNSK 1659

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
CFTR	NP_000483.3	CFTR_protein	1..1480	CDD:273530	399/1569 (25%)
		Disordered R region. /evidence=ECO:0000269|PubMed:10792060	654..831		34/178 (19%)
		Interaction with GORASP2. /evidence=ECO:0000269|PubMed:21884936	1386..1480		20/81 (25%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1452..1480		2/6 (33%)
		PDZ-binding. /evidence=ECO:0000269|PubMed:11707463, ECO:0000269|PubMed:16331976	1478..1480
YBT1	NP_013052.1	MRP_assoc_pro	<353..1639	CDD:188098	379/1469 (26%)